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Hugo W Tilanus - One of the best experts on this subject based on the ideXlab platform.

  • Migration of allosensitizing donor myeloid dendritic cells into recipients after Liver transplantation
    Liver Transplantation, 2020
    Co-Authors: Brenda M. Bosma, Herold J Metselaar, Hugo W Tilanus, Jeroen H. Gerrits, Nicole M. Van Besouw, Shanta Mancham, Zwier M. A. Groothuismink, Patrick P. C. Boor, Luc J. W. Van Der Laan, Ernst J. Kuipers
    Abstract:

    It is thought, but there is no evidence, that myeloid dendritic cells (MDCs) of donor origin migrate into the recipient after clinical organ transplantation and sensitize the recipient's immune system by the direct presentation of donor allo-antigens. Here we show prominent MDC chimerism in the recipient's circulation early after clinical Liver transplantation (LTx) but not after renal transplantation (RTx). MDCs that detach from human Liver Grafts produce large amounts of pro-inflammatory [tumor necrosis factor alpha and interleukin 6 (IL-6)] and anti-inflammatory (IL-10) cytokines upon activation with various stimuli, express higher levels of toll-like receptor 4 than blood or splenic MDCs, and are sensitive to stimulation with a physiological concentration of lipopolysaccharide (LPS). Upon stimulation with LPS, MDCs detaching from Liver Grafts prime allogeneic T cell proliferation and production of interferon gamma but not of IL-10. Soluble factors secreted by Liver Graft MDCs amplify allogeneic T helper 1 responses. In conclusion, after clinical LTx, but not after RTx, prominent numbers of donor-derived MDCs migrate into the recipient's circulation. MDCs detaching from Liver Grafts produce pro-inflammatory and anti-inflammatory cytokines and are capable of stimulating allogeneic T helper 1 responses, and this suggests that MDC chimerism after clinical LTx may contribute to Liver Graft Rejection rather than acceptance. Liver Transpl 16:12–22, 2010. © 2009 AASLD.

  • Donor and recipient HLA/KIR genotypes do not predict Liver transplantation outcome
    Transplant International, 2011
    Co-Authors: Viviana Moroso, Herold J Metselaar, Hugo W Tilanus, Luc J. W. Van Der Laan, Arnold Van Der Meer, Geert Kazemier, Irma Joosten, Jaap Kwekkeboom
    Abstract:

    Whether or not Natural Killer (NK) cells affect the immune response to solid organ alloGrafts is still controversial. Main determinants of NK-cell activation are specific HLA/killer-cell immunoglobulin-like receptors (KIR) interactions that, in transplantation, may induce NK-cell alloreactivity. So far, in Liver transplantation (LTX) donor-versus-recipient alloreactivity has not been investigated; in addition, studies of predicted recipient-versus-donor NK-cell alloreactivity have led to contradicting results. We typed a cohort of LTX donors and recipients for HLA-C/Bw4 and KIRs. We estimated the effect of NK-cell alloreactivity, as predicted by classically used models, in the donor-versus-recipient direction. The results indicate that HLA/KIR mismatches in the donor-versus-recipient direction do not predict Graft Rejection nor Graft or patient survival, suggesting that donor-derived NK cells do not play a major role in LTX outcome. In addition, when considering predicted NK-cell alloreactivity in the reverse direction (recipient-versus-donor), we first confirmed that donor HLA-C genotype was not associated with acute Rejection, Graft or patient survival and secondly we found that none of the models describing NK-cell alloreactivity could predict LTX outcome. Overall our observations suggest that, in contrast to what is shown in haematopoietic stem cell transplantation, donor-derived NK cells may not contribute in preventing Liver Graft Rejection, and that recipient-versus-donor NK-cell alloreactivity does not predict LTX outcome.

  • cytokine gene polymorphisms and acute Liver Graft Rejection a meta analysis
    Liver Transplantation, 2005
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus
    Abstract:

    In the field of Liver transplantation, 7 reports have been published investigating the association between polymorphisms in cytokine genes and the occurrence of acute Rejection in Liver Graft recipients. However, most of the individual studies lack the statistical power to detect a small-to-moderate effect of cytokine gene polymorphisms on the acute Rejection rate. To overcome this problem, we performed a quantitative meta-analysis of 7 gene-association studies that were comparable with regard to definition of acute Rejection and the type of immunosuppression used. In the overall analysis, the interleukin (IL)-10 polymorphism at position −1082 was identified as a genetic risk factor for acute Liver Graft Rejection; Liver transplant recipients carrying the IL-10 −1082.A allele displayed a lower Rejection rate (common odds ratio [OR], .6; 95% confidence interval [CI], .4-.9). For the tumor necrosis factor (TNF)-A −308 polymorphism, a common OR could not be calculated due to significant heterogeneity of ORs between the studies (mean OR, 1.4; 95% CI, .8-2.6). No associations were found between acute Liver Graft Rejection and single nucleotide polymorphisms in the IL-6 (position −174) and transforming growth factor (TGF)-β1 (positions +869 and +915) genes. In conclusion, results from this meta-analysis suggest a role for the IL-10 −1082 polymorphism in human Liver Graft Rejection. (Liver Transpl 2005;11:19–26.)

  • Cytokine gene polymorphisms and acute Liver Graft Rejection: A meta‐analysis
    Liver Transplantation, 2004
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus
    Abstract:

    In the field of Liver transplantation, 7 reports have been published investigating the association between polymorphisms in cytokine genes and the occurrence of acute Rejection in Liver Graft recipients. However, most of the individual studies lack the statistical power to detect a small-to-moderate effect of cytokine gene polymorphisms on the acute Rejection rate. To overcome this problem, we performed a quantitative meta-analysis of 7 gene-association studies that were comparable with regard to definition of acute Rejection and the type of immunosuppression used. In the overall analysis, the interleukin (IL)-10 polymorphism at position −1082 was identified as a genetic risk factor for acute Liver Graft Rejection; Liver transplant recipients carrying the IL-10 −1082.A allele displayed a lower Rejection rate (common odds ratio [OR], .6; 95% confidence interval [CI], .4-.9). For the tumor necrosis factor (TNF)-A −308 polymorphism, a common OR could not be calculated due to significant heterogeneity of ORs between the studies (mean OR, 1.4; 95% CI, .8-2.6). No associations were found between acute Liver Graft Rejection and single nucleotide polymorphisms in the IL-6 (position −174) and transforming growth factor (TGF)-β1 (positions +869 and +915) genes. In conclusion, results from this meta-analysis suggest a role for the IL-10 −1082 polymorphism in human Liver Graft Rejection. (Liver Transpl 2005;11:19–26.)

  • Early differentiation between Rejection and infection in Liver transplant patients by serum and biliary cytokine patterns.
    Transplantation, 2003
    Co-Authors: Michiel C. Warl Eacute, Herold J Metselaar, Pieter E Zondervan, Sjoerd De Rave, Jaap Kwekkeboom, Jan N M Ijzermans, Inge C. Gyssens, Hugo W Tilanus
    Abstract:

    Background. Differentiation between acute Liver Graft Rejection and infection remains a clinical challenge during the early posttransplantation period. Although cytokines play a pivotal role in mediating alloGraft Rejection, previous studies demonstrate that most cytokines are not specific for Liver Graft Rejection or infections. However, other studies suggest that adhesion molecules and cytokines in bile reflect the immunologic activity within the Liver more closely. Therefore, we postulated that by combining cytokine patterns in serum and bile, early recognition of acute Liver Graft Rejection and differentiation from infectious complications can be improved. Methods. We performed a prospective study in 45 patients who were monitored daily for clinical events and cytokine patterns in serum and bile during the first month after Liver transplantation. Results. Soluble intercellular adhesion molecule-1 (sICAM-1) in serum and interleukin-8 in bile were specifically increased at the onset of acute Rejection (P

Michiel C Warle - One of the best experts on this subject based on the ideXlab platform.

  • cytokine gene polymorphisms and acute Liver Graft Rejection a meta analysis
    Liver Transplantation, 2005
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus
    Abstract:

    In the field of Liver transplantation, 7 reports have been published investigating the association between polymorphisms in cytokine genes and the occurrence of acute Rejection in Liver Graft recipients. However, most of the individual studies lack the statistical power to detect a small-to-moderate effect of cytokine gene polymorphisms on the acute Rejection rate. To overcome this problem, we performed a quantitative meta-analysis of 7 gene-association studies that were comparable with regard to definition of acute Rejection and the type of immunosuppression used. In the overall analysis, the interleukin (IL)-10 polymorphism at position −1082 was identified as a genetic risk factor for acute Liver Graft Rejection; Liver transplant recipients carrying the IL-10 −1082.A allele displayed a lower Rejection rate (common odds ratio [OR], .6; 95% confidence interval [CI], .4-.9). For the tumor necrosis factor (TNF)-A −308 polymorphism, a common OR could not be calculated due to significant heterogeneity of ORs between the studies (mean OR, 1.4; 95% CI, .8-2.6). No associations were found between acute Liver Graft Rejection and single nucleotide polymorphisms in the IL-6 (position −174) and transforming growth factor (TGF)-β1 (positions +869 and +915) genes. In conclusion, results from this meta-analysis suggest a role for the IL-10 −1082 polymorphism in human Liver Graft Rejection. (Liver Transpl 2005;11:19–26.)

  • Cytokine gene polymorphisms and acute Liver Graft Rejection: A meta‐analysis
    Liver Transplantation, 2004
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus
    Abstract:

    In the field of Liver transplantation, 7 reports have been published investigating the association between polymorphisms in cytokine genes and the occurrence of acute Rejection in Liver Graft recipients. However, most of the individual studies lack the statistical power to detect a small-to-moderate effect of cytokine gene polymorphisms on the acute Rejection rate. To overcome this problem, we performed a quantitative meta-analysis of 7 gene-association studies that were comparable with regard to definition of acute Rejection and the type of immunosuppression used. In the overall analysis, the interleukin (IL)-10 polymorphism at position −1082 was identified as a genetic risk factor for acute Liver Graft Rejection; Liver transplant recipients carrying the IL-10 −1082.A allele displayed a lower Rejection rate (common odds ratio [OR], .6; 95% confidence interval [CI], .4-.9). For the tumor necrosis factor (TNF)-A −308 polymorphism, a common OR could not be calculated due to significant heterogeneity of ORs between the studies (mean OR, 1.4; 95% CI, .8-2.6). No associations were found between acute Liver Graft Rejection and single nucleotide polymorphisms in the IL-6 (position −174) and transforming growth factor (TGF)-β1 (positions +869 and +915) genes. In conclusion, results from this meta-analysis suggest a role for the IL-10 −1082 polymorphism in human Liver Graft Rejection. (Liver Transpl 2005;11:19–26.)

  • cytokine gene polymorphisms and acute human Liver Graft Rejection
    Liver Transplantation, 2002
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus, Ayar Farhan, Chris Perrey, Pieter E Zondervan, Sjoerd De Rave, Jaap Kwekkeboom, Jan N M Ijzermans, Vera Pravica
    Abstract:

    Interindividual differences exist in the capacity to produce cytokines. It has been reported that levels of in vitro cytokine production measured after stimulated cell culture are associated with polymorphisms in cytokine genes. Moreover, a correlation between heart, kidney, Liver, and lung Graft Rejection or survival with cytokine gene polymorphisms has been described. In the present study, we analyzed the association of gene polymorphisms in T helper subtype 1 (TH1-), TH2-, and regulatory-type cytokines with human Liver alloGraft Rejection. Patients who received a primary Liver Graft from 1992 onward and were seen at the transplant outpatient clinic since then were included on this study (n = 89). Patients were HLA typed routinely. Biopsy-proven acute Rejection occurred in 41 of 89 patients. After informed consent, blood was collected and DNA was obtained. Using amplification-refractory mutation system polymerase chain reaction, the following cytokine gene polymorphisms were determined: IL-2+166, IL-2-330, IL-15+13689, IL-15-80, TNF-A-308, TNFd3, IFN-G+874 (TH1-type cytokines), IL-4+33, IL-4-590, IL-6-174, IL-10-592, IL-10-819, IL-10-1082, IL-13+2043, IL-13-1055 (TH2 type cytokines), TGF-B1+869, and TGF-B1+915 (regulatory-type cytokines). Univariate analysis showed that polymorphisms of IL-10-1082, TGF-B1+869, and HLA-DR6 were significantly related to Liver Graft Rejection. Multiple logistic regression analysis was used to assess which variables remained significantly predictive of acute Rejection. Multivariate analysis showed that TGF-B1+869 and HLA-DR6 were independently associated with the occurrence of acute Rejection. These findings suggest a role for the regulatory-type cytokine transforming growth factor-β1 in human Liver Graft Rejection.

Raymonde Busch - One of the best experts on this subject based on the ideXlab platform.

  • diagnosing acute Liver Graft Rejection experimental application of an implantable telemetric impedance device in native and transplanted porcine Livers
    Biosensors and Bioelectronics, 2001
    Co-Authors: J Harms, Armin Schneider, Markus Baumgartner, J Henke, Raymonde Busch
    Abstract:

    Background and aims: Diagnosis of acute Rejection is a complex and persistent problem in Liver transplantation. Focused on the use of proprietary impedance technology a porcine Liver model was designed to provide immediate information for differentiation of normal and rejecting tissue by an implantable telemetric device. Methods: Electrical impedance was analysed by electrodes implanted in vitro and in vivo in the Liver of pigs, where impedance is derived from measurements of voltage transients produced in response to programmed current pulses. Consequent electric recordings in porcine Livers after transplantation and after mere laparotomy were evaluated in relation to biochemical parameters and histological results of Liver biopsies. Results: Acute Rejection was correctly predicted in all cases and excluded in the remaining 32 biopsy related impedance recordings (p<0.004). Impedance measurements not only correlated with the diagnosis from Liver biopsy specimen (r=0.84, p<0.0001) but also exemplified the severity of acute Rejection. Conclusion: Impedance analysis reveals evident physiologic relation of acute Liver Graft Rejection and electrical organ properties. Electrodes implanted in transplanted porcine Livers allow running less invasive monitoring and thus early detection of Rejection. The technology may have broad value in providing an immediate diagnosis of acute Rejection, reducing unnecessary patient anxiety and eliminating the significant expenses associated with multiple referrals, expensive sample handling and tissue analysis.

  • Diagnosing acute Liver Graft Rejection: experimental application of an implantable telemetric impedance device in native and transplanted porcine Livers
    Biosensors and Bioelectronics, 2001
    Co-Authors: J Harms, Armin Schneider, Markus Baumgartner, J Henke, Raymonde Busch
    Abstract:

    Background and aims: Diagnosis of acute Rejection is a complex and persistent problem in Liver transplantation. Focused on the use of proprietary impedance technology a porcine Liver model was designed to provide immediate information for differentiation of normal and rejecting tissue by an implantable telemetric device. Methods: Electrical impedance was analysed by electrodes implanted in vitro and in vivo in the Liver of pigs, where impedance is derived from measurements of voltage transients produced in response to programmed current pulses. Consequent electric recordings in porcine Livers after transplantation and after mere laparotomy were evaluated in relation to biochemical parameters and histological results of Liver biopsies. Results: Acute Rejection was correctly predicted in all cases and excluded in the remaining 32 biopsy related impedance recordings (p

Herold J Metselaar - One of the best experts on this subject based on the ideXlab platform.

  • Migration of allosensitizing donor myeloid dendritic cells into recipients after Liver transplantation
    Liver Transplantation, 2020
    Co-Authors: Brenda M. Bosma, Herold J Metselaar, Hugo W Tilanus, Jeroen H. Gerrits, Nicole M. Van Besouw, Shanta Mancham, Zwier M. A. Groothuismink, Patrick P. C. Boor, Luc J. W. Van Der Laan, Ernst J. Kuipers
    Abstract:

    It is thought, but there is no evidence, that myeloid dendritic cells (MDCs) of donor origin migrate into the recipient after clinical organ transplantation and sensitize the recipient's immune system by the direct presentation of donor allo-antigens. Here we show prominent MDC chimerism in the recipient's circulation early after clinical Liver transplantation (LTx) but not after renal transplantation (RTx). MDCs that detach from human Liver Grafts produce large amounts of pro-inflammatory [tumor necrosis factor alpha and interleukin 6 (IL-6)] and anti-inflammatory (IL-10) cytokines upon activation with various stimuli, express higher levels of toll-like receptor 4 than blood or splenic MDCs, and are sensitive to stimulation with a physiological concentration of lipopolysaccharide (LPS). Upon stimulation with LPS, MDCs detaching from Liver Grafts prime allogeneic T cell proliferation and production of interferon gamma but not of IL-10. Soluble factors secreted by Liver Graft MDCs amplify allogeneic T helper 1 responses. In conclusion, after clinical LTx, but not after RTx, prominent numbers of donor-derived MDCs migrate into the recipient's circulation. MDCs detaching from Liver Grafts produce pro-inflammatory and anti-inflammatory cytokines and are capable of stimulating allogeneic T helper 1 responses, and this suggests that MDC chimerism after clinical LTx may contribute to Liver Graft Rejection rather than acceptance. Liver Transpl 16:12–22, 2010. © 2009 AASLD.

  • Donor and recipient HLA/KIR genotypes do not predict Liver transplantation outcome
    Transplant International, 2011
    Co-Authors: Viviana Moroso, Herold J Metselaar, Hugo W Tilanus, Luc J. W. Van Der Laan, Arnold Van Der Meer, Geert Kazemier, Irma Joosten, Jaap Kwekkeboom
    Abstract:

    Whether or not Natural Killer (NK) cells affect the immune response to solid organ alloGrafts is still controversial. Main determinants of NK-cell activation are specific HLA/killer-cell immunoglobulin-like receptors (KIR) interactions that, in transplantation, may induce NK-cell alloreactivity. So far, in Liver transplantation (LTX) donor-versus-recipient alloreactivity has not been investigated; in addition, studies of predicted recipient-versus-donor NK-cell alloreactivity have led to contradicting results. We typed a cohort of LTX donors and recipients for HLA-C/Bw4 and KIRs. We estimated the effect of NK-cell alloreactivity, as predicted by classically used models, in the donor-versus-recipient direction. The results indicate that HLA/KIR mismatches in the donor-versus-recipient direction do not predict Graft Rejection nor Graft or patient survival, suggesting that donor-derived NK cells do not play a major role in LTX outcome. In addition, when considering predicted NK-cell alloreactivity in the reverse direction (recipient-versus-donor), we first confirmed that donor HLA-C genotype was not associated with acute Rejection, Graft or patient survival and secondly we found that none of the models describing NK-cell alloreactivity could predict LTX outcome. Overall our observations suggest that, in contrast to what is shown in haematopoietic stem cell transplantation, donor-derived NK cells may not contribute in preventing Liver Graft Rejection, and that recipient-versus-donor NK-cell alloreactivity does not predict LTX outcome.

  • cytokine gene polymorphisms and acute Liver Graft Rejection a meta analysis
    Liver Transplantation, 2005
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus
    Abstract:

    In the field of Liver transplantation, 7 reports have been published investigating the association between polymorphisms in cytokine genes and the occurrence of acute Rejection in Liver Graft recipients. However, most of the individual studies lack the statistical power to detect a small-to-moderate effect of cytokine gene polymorphisms on the acute Rejection rate. To overcome this problem, we performed a quantitative meta-analysis of 7 gene-association studies that were comparable with regard to definition of acute Rejection and the type of immunosuppression used. In the overall analysis, the interleukin (IL)-10 polymorphism at position −1082 was identified as a genetic risk factor for acute Liver Graft Rejection; Liver transplant recipients carrying the IL-10 −1082.A allele displayed a lower Rejection rate (common odds ratio [OR], .6; 95% confidence interval [CI], .4-.9). For the tumor necrosis factor (TNF)-A −308 polymorphism, a common OR could not be calculated due to significant heterogeneity of ORs between the studies (mean OR, 1.4; 95% CI, .8-2.6). No associations were found between acute Liver Graft Rejection and single nucleotide polymorphisms in the IL-6 (position −174) and transforming growth factor (TGF)-β1 (positions +869 and +915) genes. In conclusion, results from this meta-analysis suggest a role for the IL-10 −1082 polymorphism in human Liver Graft Rejection. (Liver Transpl 2005;11:19–26.)

  • Cytokine gene polymorphisms and acute Liver Graft Rejection: A meta‐analysis
    Liver Transplantation, 2004
    Co-Authors: Michiel C Warle, Herold J Metselaar, Hugo W Tilanus
    Abstract:

    In the field of Liver transplantation, 7 reports have been published investigating the association between polymorphisms in cytokine genes and the occurrence of acute Rejection in Liver Graft recipients. However, most of the individual studies lack the statistical power to detect a small-to-moderate effect of cytokine gene polymorphisms on the acute Rejection rate. To overcome this problem, we performed a quantitative meta-analysis of 7 gene-association studies that were comparable with regard to definition of acute Rejection and the type of immunosuppression used. In the overall analysis, the interleukin (IL)-10 polymorphism at position −1082 was identified as a genetic risk factor for acute Liver Graft Rejection; Liver transplant recipients carrying the IL-10 −1082.A allele displayed a lower Rejection rate (common odds ratio [OR], .6; 95% confidence interval [CI], .4-.9). For the tumor necrosis factor (TNF)-A −308 polymorphism, a common OR could not be calculated due to significant heterogeneity of ORs between the studies (mean OR, 1.4; 95% CI, .8-2.6). No associations were found between acute Liver Graft Rejection and single nucleotide polymorphisms in the IL-6 (position −174) and transforming growth factor (TGF)-β1 (positions +869 and +915) genes. In conclusion, results from this meta-analysis suggest a role for the IL-10 −1082 polymorphism in human Liver Graft Rejection. (Liver Transpl 2005;11:19–26.)

  • Early differentiation between Rejection and infection in Liver transplant patients by serum and biliary cytokine patterns.
    Transplantation, 2003
    Co-Authors: Michiel C. Warl Eacute, Herold J Metselaar, Pieter E Zondervan, Sjoerd De Rave, Jaap Kwekkeboom, Jan N M Ijzermans, Inge C. Gyssens, Hugo W Tilanus
    Abstract:

    Background. Differentiation between acute Liver Graft Rejection and infection remains a clinical challenge during the early posttransplantation period. Although cytokines play a pivotal role in mediating alloGraft Rejection, previous studies demonstrate that most cytokines are not specific for Liver Graft Rejection or infections. However, other studies suggest that adhesion molecules and cytokines in bile reflect the immunologic activity within the Liver more closely. Therefore, we postulated that by combining cytokine patterns in serum and bile, early recognition of acute Liver Graft Rejection and differentiation from infectious complications can be improved. Methods. We performed a prospective study in 45 patients who were monitored daily for clinical events and cytokine patterns in serum and bile during the first month after Liver transplantation. Results. Soluble intercellular adhesion molecule-1 (sICAM-1) in serum and interleukin-8 in bile were specifically increased at the onset of acute Rejection (P

R Williams - One of the best experts on this subject based on the ideXlab platform.

  • quantitative assessment of serum beta 2 microglobulin in Liver transplant recipients and relationship to Liver Graft Rejection
    European Journal of Gastroenterology & Hepatology, 1995
    Co-Authors: Marco Vivarelli, Heather M Smith, Nikolai V Naoumov, R Williams
    Abstract:

    Objective : To investigate the usefulness of serum β 2 -microglobulin determination in the diagnosis of acute Liver alloGraft Rejection. Design : Prospective study. Setting : Liver transplant unit. Patients : Twenty consecutive patients who underwent Liver transplantation because of a non-virus-related end-stage Liver disease. Methods : Serum samples were collected before the transplant, at days 7, 30 and 90 and whenever a clinical complication developed after Liver transplantation. β 2 -Microglobulin was quantified using a new quantitative automated microparticle enzyme immunoassay. Results : Serum β 2 -microglobulin levels increased significantly (P<0.05) during Rejection episodes and correlated with the degree of hepatocyte injury as assessed using serum aspartate aminotransferase levels. Increased β 2 -microglobulin levels were also found in surgical or infectious post-transplant complications. A significant difference in β 2 -microglobulin values was recorded between patients with Rejection and only those with bacterial sepsis. Conclusion : Although highly sensitive in recognizing damage to the Graft, determination of β 2 -microglobulin was not sufficiently specific to differentiate between Rejection and other post-transplantation complications.

  • Quantitative assessment of serum beta-2-microglobulin in Liver transplant recipients and relationship to Liver Graft Rejection.
    European Journal of Gastroenterology & Hepatology, 1995
    Co-Authors: Marco Vivarelli, Heather M Smith, Nikolai V Naoumov, R Williams
    Abstract:

    Objective : To investigate the usefulness of serum β 2 -microglobulin determination in the diagnosis of acute Liver alloGraft Rejection. Design : Prospective study. Setting : Liver transplant unit. Patients : Twenty consecutive patients who underwent Liver transplantation because of a non-virus-related end-stage Liver disease. Methods : Serum samples were collected before the transplant, at days 7, 30 and 90 and whenever a clinical complication developed after Liver transplantation. β 2 -Microglobulin was quantified using a new quantitative automated microparticle enzyme immunoassay. Results : Serum β 2 -microglobulin levels increased significantly (P