The Experts below are selected from a list of 1086 Experts worldwide ranked by ideXlab platform
Albo S - One of the best experts on this subject based on the ideXlab platform.
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Rat Liver Lysosome membranes are enriched in alpha-tocopherol.
1992Co-Authors: Albo SAbstract:The subcellular distribution of alpha-tocopherol has been studied in rat Liver. Lysosomal membranes were found to be considerably enriched in alpha-tocopherol with 6300 pmol/mg membrane protein, whereas mitochondrial membranes and microsomes contained 530 and 200 pmol/mg membrane protein, respectively. The 37-fold higher specific content of alpha-tocopherol in lysosomal membranes relative to homogenate indicates that Lysosomes could be a target of cellular pathology in vitamin E deficiency states.
Xiaqing Wang - One of the best experts on this subject based on the ideXlab platform.
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Effect of cholera toxin on rat Liver Lysosome acidification.
2000Co-Authors: Rebecca W. Van Dyke, Loretha L. Ervin, Marianne R. Lewis, Xiaqing WangAbstract:We have shown that cholera toxin and cAMP greatly increase both acidification rates of Liver endosomes and the Liver and endosome content of fluid-phase endocytosis probes. In this study Lysosomes were purified from control and cholera toxin-treated Livers that were pulsed with fluorescein conjugated dextran and chased overnight. Cholera toxin-treated Livers weighed less, contained less protein and exhibited higher contents of lysosomal marker enzymes, consistent with the catabolic effects of this agent. By contrast to its effects on endosomes, cholera toxin had no consistent or significant effect on Lysosome acidification rates, steady-state internal pH or potassium content, proton leak rates or fluorescein-dextran content. We conclude that cholera toxin and cAMP predominantly alter earlier steps of endocytosis but may also increase transfer of probes from Lysosomes to bile.
Rebecca W. Van Dyke - One of the best experts on this subject based on the ideXlab platform.
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Effect of cholera toxin on rat Liver Lysosome acidification.
2000Co-Authors: Rebecca W. Van Dyke, Loretha L. Ervin, Marianne R. Lewis, Xiaqing WangAbstract:We have shown that cholera toxin and cAMP greatly increase both acidification rates of Liver endosomes and the Liver and endosome content of fluid-phase endocytosis probes. In this study Lysosomes were purified from control and cholera toxin-treated Livers that were pulsed with fluorescein conjugated dextran and chased overnight. Cholera toxin-treated Livers weighed less, contained less protein and exhibited higher contents of lysosomal marker enzymes, consistent with the catabolic effects of this agent. By contrast to its effects on endosomes, cholera toxin had no consistent or significant effect on Lysosome acidification rates, steady-state internal pH or potassium content, proton leak rates or fluorescein-dextran content. We conclude that cholera toxin and cAMP predominantly alter earlier steps of endocytosis but may also increase transfer of probes from Lysosomes to bile.
Loretha L. Ervin - One of the best experts on this subject based on the ideXlab platform.
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Effect of cholera toxin on rat Liver Lysosome acidification.
2000Co-Authors: Rebecca W. Van Dyke, Loretha L. Ervin, Marianne R. Lewis, Xiaqing WangAbstract:We have shown that cholera toxin and cAMP greatly increase both acidification rates of Liver endosomes and the Liver and endosome content of fluid-phase endocytosis probes. In this study Lysosomes were purified from control and cholera toxin-treated Livers that were pulsed with fluorescein conjugated dextran and chased overnight. Cholera toxin-treated Livers weighed less, contained less protein and exhibited higher contents of lysosomal marker enzymes, consistent with the catabolic effects of this agent. By contrast to its effects on endosomes, cholera toxin had no consistent or significant effect on Lysosome acidification rates, steady-state internal pH or potassium content, proton leak rates or fluorescein-dextran content. We conclude that cholera toxin and cAMP predominantly alter earlier steps of endocytosis but may also increase transfer of probes from Lysosomes to bile.
Marianne R. Lewis - One of the best experts on this subject based on the ideXlab platform.
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Effect of cholera toxin on rat Liver Lysosome acidification.
2000Co-Authors: Rebecca W. Van Dyke, Loretha L. Ervin, Marianne R. Lewis, Xiaqing WangAbstract:We have shown that cholera toxin and cAMP greatly increase both acidification rates of Liver endosomes and the Liver and endosome content of fluid-phase endocytosis probes. In this study Lysosomes were purified from control and cholera toxin-treated Livers that were pulsed with fluorescein conjugated dextran and chased overnight. Cholera toxin-treated Livers weighed less, contained less protein and exhibited higher contents of lysosomal marker enzymes, consistent with the catabolic effects of this agent. By contrast to its effects on endosomes, cholera toxin had no consistent or significant effect on Lysosome acidification rates, steady-state internal pH or potassium content, proton leak rates or fluorescein-dextran content. We conclude that cholera toxin and cAMP predominantly alter earlier steps of endocytosis but may also increase transfer of probes from Lysosomes to bile.