The Experts below are selected from a list of 234129 Experts worldwide ranked by ideXlab platform

Isabel Medina - One of the best experts on this subject based on the ideXlab platform.

  • modulation of the Liver Protein carbonylome by the combined effect of marine omega 3 pufas and grape polyphenols supplementation in rats fed an obesogenic high fat and high sucrose diet
    Marine Drugs, 2019
    Co-Authors: Lucia Mendez, Josep Lluis Torres, Silvia Muñoz, María Rosa Nogués, Sara Ramosromero, Bernat Mirallesperez, Isabel Medina
    Abstract:

    Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially Proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified Proteins from plasma and Liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of Protein carbonyls, visualization of FTSC-labelled Protein on 1-DE or 2-DE gels, and Protein identification by MS/MS was used for the Protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total Protein carbonylation induced by HFHS diet in both plasma and Liver. The analysis of carbonylated Protein targets, also referred to as the ‘carbonylome’, revealed an individual response of Liver Proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the Liver Protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of Proteins induced by the consumption of HFHS diets.

  • targeting hepatic Protein carbonylation and oxidative stress occurring on diet induced metabolic diseases through the supplementation with fish oils
    Marine Drugs, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Marta Romeu, María Rosa Nogués, Sara Ramosromero, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver.

  • Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils
    MDPI AG, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Sara Ramos-romero, Marta Romeu, María Rosa Nogués, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver

Lucia Mendez - One of the best experts on this subject based on the ideXlab platform.

  • modulation of the Liver Protein carbonylome by the combined effect of marine omega 3 pufas and grape polyphenols supplementation in rats fed an obesogenic high fat and high sucrose diet
    Marine Drugs, 2019
    Co-Authors: Lucia Mendez, Josep Lluis Torres, Silvia Muñoz, María Rosa Nogués, Sara Ramosromero, Bernat Mirallesperez, Isabel Medina
    Abstract:

    Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially Proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified Proteins from plasma and Liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of Protein carbonyls, visualization of FTSC-labelled Protein on 1-DE or 2-DE gels, and Protein identification by MS/MS was used for the Protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total Protein carbonylation induced by HFHS diet in both plasma and Liver. The analysis of carbonylated Protein targets, also referred to as the ‘carbonylome’, revealed an individual response of Liver Proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the Liver Protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of Proteins induced by the consumption of HFHS diets.

  • targeting hepatic Protein carbonylation and oxidative stress occurring on diet induced metabolic diseases through the supplementation with fish oils
    Marine Drugs, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Marta Romeu, María Rosa Nogués, Sara Ramosromero, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver.

  • Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils
    MDPI AG, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Sara Ramos-romero, Marta Romeu, María Rosa Nogués, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver

Silvia Muñoz - One of the best experts on this subject based on the ideXlab platform.

  • modulation of the Liver Protein carbonylome by the combined effect of marine omega 3 pufas and grape polyphenols supplementation in rats fed an obesogenic high fat and high sucrose diet
    Marine Drugs, 2019
    Co-Authors: Lucia Mendez, Josep Lluis Torres, Silvia Muñoz, María Rosa Nogués, Sara Ramosromero, Bernat Mirallesperez, Isabel Medina
    Abstract:

    Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially Proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified Proteins from plasma and Liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of Protein carbonyls, visualization of FTSC-labelled Protein on 1-DE or 2-DE gels, and Protein identification by MS/MS was used for the Protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total Protein carbonylation induced by HFHS diet in both plasma and Liver. The analysis of carbonylated Protein targets, also referred to as the ‘carbonylome’, revealed an individual response of Liver Proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the Liver Protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of Proteins induced by the consumption of HFHS diets.

  • targeting hepatic Protein carbonylation and oxidative stress occurring on diet induced metabolic diseases through the supplementation with fish oils
    Marine Drugs, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Marta Romeu, María Rosa Nogués, Sara Ramosromero, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver.

  • Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils
    MDPI AG, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Sara Ramos-romero, Marta Romeu, María Rosa Nogués, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver

Sara Ramosromero - One of the best experts on this subject based on the ideXlab platform.

  • modulation of the Liver Protein carbonylome by the combined effect of marine omega 3 pufas and grape polyphenols supplementation in rats fed an obesogenic high fat and high sucrose diet
    Marine Drugs, 2019
    Co-Authors: Lucia Mendez, Josep Lluis Torres, Silvia Muñoz, María Rosa Nogués, Sara Ramosromero, Bernat Mirallesperez, Isabel Medina
    Abstract:

    Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially Proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified Proteins from plasma and Liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of Protein carbonyls, visualization of FTSC-labelled Protein on 1-DE or 2-DE gels, and Protein identification by MS/MS was used for the Protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total Protein carbonylation induced by HFHS diet in both plasma and Liver. The analysis of carbonylated Protein targets, also referred to as the ‘carbonylome’, revealed an individual response of Liver Proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the Liver Protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of Proteins induced by the consumption of HFHS diets.

  • targeting hepatic Protein carbonylation and oxidative stress occurring on diet induced metabolic diseases through the supplementation with fish oils
    Marine Drugs, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Marta Romeu, María Rosa Nogués, Sara Ramosromero, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver.

Josep Lluis Torres - One of the best experts on this subject based on the ideXlab platform.

  • modulation of the Liver Protein carbonylome by the combined effect of marine omega 3 pufas and grape polyphenols supplementation in rats fed an obesogenic high fat and high sucrose diet
    Marine Drugs, 2019
    Co-Authors: Lucia Mendez, Josep Lluis Torres, Silvia Muñoz, María Rosa Nogués, Sara Ramosromero, Bernat Mirallesperez, Isabel Medina
    Abstract:

    Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially Proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified Proteins from plasma and Liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of Protein carbonyls, visualization of FTSC-labelled Protein on 1-DE or 2-DE gels, and Protein identification by MS/MS was used for the Protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total Protein carbonylation induced by HFHS diet in both plasma and Liver. The analysis of carbonylated Protein targets, also referred to as the ‘carbonylome’, revealed an individual response of Liver Proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the Liver Protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of Proteins induced by the consumption of HFHS diets.

  • targeting hepatic Protein carbonylation and oxidative stress occurring on diet induced metabolic diseases through the supplementation with fish oils
    Marine Drugs, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Marta Romeu, María Rosa Nogués, Sara Ramosromero, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver.

  • Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils
    MDPI AG, 2018
    Co-Authors: Silvia Muñoz, Lucia Mendez, Josep Lluis Torres, Gabriel Dasilva, Sara Ramos-romero, Marta Romeu, María Rosa Nogués, Isabel Medina
    Abstract:

    The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate Liver Protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated Proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased Liver Protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty Liver Proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the Liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in Liver