The Experts below are selected from a list of 33 Experts worldwide ranked by ideXlab platform
Jean Mariani - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis of the purkinje cell population during extreme ageing in the cerebellum of the wistar Louvain Rat
Neurobiology of Aging, 1991Co-Authors: Andre Bakalian, Bruno Corman, Nicole Delhayebouchaud, Jean MarianiAbstract:Abstract The loss of neurons is viewed as one of several causes of the deterioRation of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degeneRative pathology of the animals studied. To reinvestigate this question we have quantified an easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy Rats. The number of Purkinje cells in the cerebellum was assessed in two populations of Rats: control (10 months) and old (42 months) Rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250×. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean±standard deviation): 12.8±1.16 mm (n=6) for the control Rats and 12.0±1.02 mm for the old animals (n=8) (Student's t-test, p=0.18). The corrected number of the Purkinje cells (mean±standard deviation) was 330, 350±35,448 cells (n=6) for the control animals and 299,019±50,223 (n=8) cells for the old Rats. The 9.5% reduction observed in aged animals was not statistically significant (p=0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain Rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
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Quantitative analysis of the Purkinje cell population during extreme ageing in the cerebellum of the Wistar/Louvain Rat.
Neurobiology of aging, 1991Co-Authors: Andre Bakalian, Bruno Corman, Nicole Delhaye-bouchaud, Jean MarianiAbstract:Abstract The loss of neurons is viewed as one of several causes of the deterioRation of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degeneRative pathology of the animals studied. To reinvestigate this question we have quantified an easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy Rats. The number of Purkinje cells in the cerebellum was assessed in two populations of Rats: control (10 months) and old (42 months) Rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250×. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean±standard deviation): 12.8±1.16 mm (n=6) for the control Rats and 12.0±1.02 mm for the old animals (n=8) (Student's t-test, p=0.18). The corrected number of the Purkinje cells (mean±standard deviation) was 330, 350±35,448 cells (n=6) for the control animals and 299,019±50,223 (n=8) cells for the old Rats. The 9.5% reduction observed in aged animals was not statistically significant (p=0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain Rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
Andre Bakalian - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis of the purkinje cell population during extreme ageing in the cerebellum of the wistar Louvain Rat
Neurobiology of Aging, 1991Co-Authors: Andre Bakalian, Bruno Corman, Nicole Delhayebouchaud, Jean MarianiAbstract:Abstract The loss of neurons is viewed as one of several causes of the deterioRation of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degeneRative pathology of the animals studied. To reinvestigate this question we have quantified an easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy Rats. The number of Purkinje cells in the cerebellum was assessed in two populations of Rats: control (10 months) and old (42 months) Rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250×. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean±standard deviation): 12.8±1.16 mm (n=6) for the control Rats and 12.0±1.02 mm for the old animals (n=8) (Student's t-test, p=0.18). The corrected number of the Purkinje cells (mean±standard deviation) was 330, 350±35,448 cells (n=6) for the control animals and 299,019±50,223 (n=8) cells for the old Rats. The 9.5% reduction observed in aged animals was not statistically significant (p=0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain Rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
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Quantitative analysis of the Purkinje cell population during extreme ageing in the cerebellum of the Wistar/Louvain Rat.
Neurobiology of aging, 1991Co-Authors: Andre Bakalian, Bruno Corman, Nicole Delhaye-bouchaud, Jean MarianiAbstract:Abstract The loss of neurons is viewed as one of several causes of the deterioRation of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degeneRative pathology of the animals studied. To reinvestigate this question we have quantified an easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy Rats. The number of Purkinje cells in the cerebellum was assessed in two populations of Rats: control (10 months) and old (42 months) Rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250×. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean±standard deviation): 12.8±1.16 mm (n=6) for the control Rats and 12.0±1.02 mm for the old animals (n=8) (Student's t-test, p=0.18). The corrected number of the Purkinje cells (mean±standard deviation) was 330, 350±35,448 cells (n=6) for the control animals and 299,019±50,223 (n=8) cells for the old Rats. The 9.5% reduction observed in aged animals was not statistically significant (p=0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain Rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
Bruno Corman - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis of the purkinje cell population during extreme ageing in the cerebellum of the wistar Louvain Rat
Neurobiology of Aging, 1991Co-Authors: Andre Bakalian, Bruno Corman, Nicole Delhayebouchaud, Jean MarianiAbstract:Abstract The loss of neurons is viewed as one of several causes of the deterioRation of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degeneRative pathology of the animals studied. To reinvestigate this question we have quantified an easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy Rats. The number of Purkinje cells in the cerebellum was assessed in two populations of Rats: control (10 months) and old (42 months) Rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250×. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean±standard deviation): 12.8±1.16 mm (n=6) for the control Rats and 12.0±1.02 mm for the old animals (n=8) (Student's t-test, p=0.18). The corrected number of the Purkinje cells (mean±standard deviation) was 330, 350±35,448 cells (n=6) for the control animals and 299,019±50,223 (n=8) cells for the old Rats. The 9.5% reduction observed in aged animals was not statistically significant (p=0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain Rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
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Quantitative analysis of the Purkinje cell population during extreme ageing in the cerebellum of the Wistar/Louvain Rat.
Neurobiology of aging, 1991Co-Authors: Andre Bakalian, Bruno Corman, Nicole Delhaye-bouchaud, Jean MarianiAbstract:Abstract The loss of neurons is viewed as one of several causes of the deterioRation of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degeneRative pathology of the animals studied. To reinvestigate this question we have quantified an easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy Rats. The number of Purkinje cells in the cerebellum was assessed in two populations of Rats: control (10 months) and old (42 months) Rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250×. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean±standard deviation): 12.8±1.16 mm (n=6) for the control Rats and 12.0±1.02 mm for the old animals (n=8) (Student's t-test, p=0.18). The corrected number of the Purkinje cells (mean±standard deviation) was 330, 350±35,448 cells (n=6) for the control animals and 299,019±50,223 (n=8) cells for the old Rats. The 9.5% reduction observed in aged animals was not statistically significant (p=0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain Rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
George Klein - One of the best experts on this subject based on the ideXlab platform.
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Three exceptional IgH/myc-translocation-carrying Rat immunocytomas have breakpoints 50 to 80 kb 5' of c-myc
International journal of cancer, 1994Co-Authors: Håkan Axelson, Chinmay Kumar Panda, George KleinAbstract:The spontaneously arising immunocytoma of the Louvain Rat (RIC) carries a consistent chromosomal translocation between chromosomes 6 and 7. This translocation juxtaposes immunoglobulin heavy chain and c-myc sequences. In an earlier study on 14 RIC tumors, we found that the translocation breakpoint is located within 1.5 kb immediately upstream of c-myc in 10 of the tumors. Here we describe 3 exceptional tumors that had no rearrangement within 20 kb 5' of c-myc. Using pulsed-field gel electrophoresis we show that the translocation breakpoints in these tumors are located 50-80 kb 5' of c-myc and that c-myc rearranges to the 3' end of the IgH cluster.
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Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying Rat immunocytomas.
Genes chromosomes & cancer, 1991Co-Authors: Håkan Axelson, Hervé Bazin, Warren S. Pear, Chinmay Kumar Panda, George Klein, Janos SümegiAbstract:We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain Rat (RIC) leads to the juxtaposition of myc to the IgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run-on analysis showed transcriptional attenuation in the normal Rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon 1, as found by others in Burkitt's lymphoma. We also show that the transcriptional initiation of myc mRNA is changed in the RICs. In an established line of Rat fibroblasts (Rat-2), the more distal myc promoter (P2) is the preferred site of initiation. In RIC, however, only 30% of transcripts were initiated from P2. We found that 40% of the transcripts were initiated from P1 and 30% from a novel promoter, designated P1a, located between P1 and P2.
Håkan Axelson - One of the best experts on this subject based on the ideXlab platform.
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Three exceptional IgH/myc-translocation-carrying Rat immunocytomas have breakpoints 50 to 80 kb 5' of c-myc
International journal of cancer, 1994Co-Authors: Håkan Axelson, Chinmay Kumar Panda, George KleinAbstract:The spontaneously arising immunocytoma of the Louvain Rat (RIC) carries a consistent chromosomal translocation between chromosomes 6 and 7. This translocation juxtaposes immunoglobulin heavy chain and c-myc sequences. In an earlier study on 14 RIC tumors, we found that the translocation breakpoint is located within 1.5 kb immediately upstream of c-myc in 10 of the tumors. Here we describe 3 exceptional tumors that had no rearrangement within 20 kb 5' of c-myc. Using pulsed-field gel electrophoresis we show that the translocation breakpoints in these tumors are located 50-80 kb 5' of c-myc and that c-myc rearranges to the 3' end of the IgH cluster.
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Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying Rat immunocytomas.
Genes chromosomes & cancer, 1991Co-Authors: Håkan Axelson, Hervé Bazin, Warren S. Pear, Chinmay Kumar Panda, George Klein, Janos SümegiAbstract:We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain Rat (RIC) leads to the juxtaposition of myc to the IgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run-on analysis showed transcriptional attenuation in the normal Rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon 1, as found by others in Burkitt's lymphoma. We also show that the transcriptional initiation of myc mRNA is changed in the RICs. In an established line of Rat fibroblasts (Rat-2), the more distal myc promoter (P2) is the preferred site of initiation. In RIC, however, only 30% of transcripts were initiated from P2. We found that 40% of the transcripts were initiated from P1 and 30% from a novel promoter, designated P1a, located between P1 and P2.
