The Experts below are selected from a list of 91236 Experts worldwide ranked by ideXlab platform
Stephen M Lane - One of the best experts on this subject based on the ideXlab platform.
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image reconstruction for structured illumination microscopy with Low Signal Level
Optics Express, 2014Co-Authors: Paul J Mcmillan, Zachary J Smith, Jeniffer Atkins, Paul C Goodwin, Sebastian Wachsmannhogiu, Stephen M LaneAbstract:We report a new image processing technique for the structured illumination microscopy designed to work with Low Signals, with the goal of reducing photobleaching and phototoxicity of the sample. Using a pre-filtering process to estimate experimental parameters and total variation as a constraint to reconstruct, we obtain two orders of magnitude of exposure reduction while maintaining the resolution improvement and image quality compared to a standard structured illumination microscopy. The algorithm is validated on both fixed and live cell data with results confirming that we can image more than 15x more time points compared to the standard technique.
S E Pollack - One of the best experts on this subject based on the ideXlab platform.
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demonstration of the zero crossing phasemeter with a lisa test bed interferometer
Classical and Quantum Gravity, 2006Co-Authors: S E Pollack, R T StebbinsAbstract:The laser interferometer space antenna (LISA) is being designed to detect and study in detail gravitational waves from sources throughout the Universe such as massive black hole binaries. The conceptual formulation of the LISA space-borne gravitational wave detector is now well developed. The interferometric measurements between the sciencecraft remain one of the most important technological and scientific design areas for the mission. Our work has concentrated on developing the interferometric technologies to create a LISA-like optical Signal and to measure the phase of that Signal using commercially available instruments. One of the most important goals of this research is to demonstrate the LISA phase timing and phase reconstruction for a LISA-like fringe Signal, in the case of a high fringe rate and a Low Signal Level. We present current results of a test-bed interferometer designed to produce an optical LISA-like fringe Signal previously discussed in Jennrich O, Stebbins R T, Bender P L and Pollack S (2001 Class. Quantum Grav. 18 4159–64) and Pollack S E, Jennrich O, Stebbins R T and Bender P (2003 Class. Quantum Grav. 20 S291–00).
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demonstration of the zero crossing phasemeter with a lisa test bed interferometer
arXiv: General Relativity and Quantum Cosmology, 2006Co-Authors: S E Pollack, R T StebbinsAbstract:The Laser Interferometer Space Antenna (LISA) is being designed to detect and study in detail gravitational waves from sources throughout the Universe such as massive black hole binaries. The conceptual formulation of the LISA space-borne gravitational wave detector is now well developed. The interferometric measurements between the sciencecraft remain one of the most important technological and scientific design areas for the mission. Our work has concentrated on developing the interferometric technologies to create a LISA-like optical Signal and to measure the phase of that Signal using commercially available instruments. One of the most important goals of this research is to demonstrate the LISA phase timing and phase reconstruction for a LISA-like fringe Signal, in the case of a high fringe rate and a Low Signal Level. We present current results of a test-bed interferometer designed to produce an optical LISA-like fringe Signal previously discussed in the literature.
Paul J Mcmillan - One of the best experts on this subject based on the ideXlab platform.
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image reconstruction for structured illumination microscopy with Low Signal Level
Optics Express, 2014Co-Authors: Paul J Mcmillan, Zachary J Smith, Jeniffer Atkins, Paul C Goodwin, Sebastian Wachsmannhogiu, Stephen M LaneAbstract:We report a new image processing technique for the structured illumination microscopy designed to work with Low Signals, with the goal of reducing photobleaching and phototoxicity of the sample. Using a pre-filtering process to estimate experimental parameters and total variation as a constraint to reconstruct, we obtain two orders of magnitude of exposure reduction while maintaining the resolution improvement and image quality compared to a standard structured illumination microscopy. The algorithm is validated on both fixed and live cell data with results confirming that we can image more than 15x more time points compared to the standard technique.
Carmen Bartic - One of the best experts on this subject based on the ideXlab platform.
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Second-order optimized regularized structured illumination microscopy (sorSIM) for high-quality and rapid super resolution image reconstruction with Low Signal Level
Optics express, 2020Co-Authors: Stijn Jooken, Olivier Deschaume, Fei Liu, Shouyu Wang, Carmen BarticAbstract:Structured illumination microscopy (SIM) is a widely used super resolution imaging technique that can down-modulate a sample’s high-frequency information into objective recordable frequencies to enhance the resolution beLow the diffraction limit. However, classical SIM image reconstruction methods often generate poor results under Low illumination conditions, which are required for reducing photobleaching and phototoxicity in cell imaging experiments. Although denoising methods or auxiliary items improved SIM image reconstruction in Low Signal Level situations, they still suffer from decreased reconstruction quality and significant background artifacts, inevitably limiting their practical applications. In order to improve the reconstruction quality, second-order optimized regularized SIM (sorSIM) is designed specifically for image reconstruction in Low Signal Level situations. In sorSIM, a second-order regularization term is introduced to suppress noise effect, and the penalty factor in this term is selected to optimize the resolution enhancement and noise resistance. Compared to classical SIM image reconstruction algorithms as well as to those previously used in Low illumination cases, the proposed sorSIM provides images with enhanced resolution and fewer background artifacts. Therefore, sorSIM can be a potential tool for high-quality and rapid super resolution imaging, especially for Low Signal images.
R T Stebbins - One of the best experts on this subject based on the ideXlab platform.
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demonstration of the zero crossing phasemeter with a lisa test bed interferometer
Classical and Quantum Gravity, 2006Co-Authors: S E Pollack, R T StebbinsAbstract:The laser interferometer space antenna (LISA) is being designed to detect and study in detail gravitational waves from sources throughout the Universe such as massive black hole binaries. The conceptual formulation of the LISA space-borne gravitational wave detector is now well developed. The interferometric measurements between the sciencecraft remain one of the most important technological and scientific design areas for the mission. Our work has concentrated on developing the interferometric technologies to create a LISA-like optical Signal and to measure the phase of that Signal using commercially available instruments. One of the most important goals of this research is to demonstrate the LISA phase timing and phase reconstruction for a LISA-like fringe Signal, in the case of a high fringe rate and a Low Signal Level. We present current results of a test-bed interferometer designed to produce an optical LISA-like fringe Signal previously discussed in Jennrich O, Stebbins R T, Bender P L and Pollack S (2001 Class. Quantum Grav. 18 4159–64) and Pollack S E, Jennrich O, Stebbins R T and Bender P (2003 Class. Quantum Grav. 20 S291–00).
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demonstration of the zero crossing phasemeter with a lisa test bed interferometer
arXiv: General Relativity and Quantum Cosmology, 2006Co-Authors: S E Pollack, R T StebbinsAbstract:The Laser Interferometer Space Antenna (LISA) is being designed to detect and study in detail gravitational waves from sources throughout the Universe such as massive black hole binaries. The conceptual formulation of the LISA space-borne gravitational wave detector is now well developed. The interferometric measurements between the sciencecraft remain one of the most important technological and scientific design areas for the mission. Our work has concentrated on developing the interferometric technologies to create a LISA-like optical Signal and to measure the phase of that Signal using commercially available instruments. One of the most important goals of this research is to demonstrate the LISA phase timing and phase reconstruction for a LISA-like fringe Signal, in the case of a high fringe rate and a Low Signal Level. We present current results of a test-bed interferometer designed to produce an optical LISA-like fringe Signal previously discussed in the literature.
