The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
G. Oehler - One of the best experts on this subject based on the ideXlab platform.
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Isolation ofChlamydia trachomatis from the Lower Digestive Tract
Infection, 1993Co-Authors: Bernhard Zöllner, R. Laufs, H. J. Stellbrink, H. Koch, L. Iske, H. -h. Feucht, G. OehlerAbstract:Chlamydia trachomatis was isolated from ten of 188 biopsies (5.3%) obtained from different parts of the Lower Digestive Tract. Patients (mean age 37.0 years) presented with ulcerative proctitis, Crohn's disease, mild colitis or ulcerative colitis. Seven rectal biopsies, two biopsies from the sigmoid flexure and one caecal biopsy were positive for chlamydial isolation whereas all biopsies taken from the colon ascendens, transversum or descendens and from the terminal ileum were negative. We conclude that isolation of C. trachomatis is most effective from rectal and sigmoidal biopsies and is a rare event from other sites of the Lower Digestive Tract. Chlamydia trachomatis wurde aus zehn von 188 Biopsaten (5,3%) isoliert, die aus verschiedenen Regionen des unteren Verdauungstraktes entnommen wurden. Die Diagnosen der Patienten (Durchschnittsalter 37,0 Jahre) waren: ulzerative Proktitis, Morbus Crohn, milde Colitis oder Colitis ulcerosa. Die Chlamydien-Isolierung war positiv bei sieben rektalen Biopsaten, zwei Biopsaten aus dem Sigma und einem Biopsat aus dem Zoekum, während alle Biopsate aus dem Colon ascendens, transversum oder descendens und dem terminalen Ileum negativ waren. Wir schließen daraus, daß die Isolierung von C. trachomatis am effektivsten aus Rektum und Sigma ist und ein seltenes Ereignis aus anderen Regionen des unteren Verdauungstraktes darstellt.
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Isolation of Chlamydia trachomatis from the Lower Digestive Tract
Infection, 1993Co-Authors: Bernhard Zöllner, R. Laufs, H. J. Stellbrink, H. Koch, L. Iske, G. OehlerAbstract:Chlamydia trachomatis was isolated from ten of 188 biopsies (5.3%) obtained from different parts of the Lower Digestive Tract. Patients (mean age 37.0 years) presented with ulcerative proctitis, Crohn's disease, mild colitis or ulcerative colitis. Seven rectal biopsies, two biopsies from the sigmoid flexure and one caecal biopsy were positive for chlamydial isolation whereas all biopsies taken from the colon ascendens, transversum or descendens and from the terminal ileum were negative. We conclude that isolation ofC. trachomatis is most effective from rectal and sigmoidal biopsies and is a rare event from other sites of the Lower Digestive Tract.
Bernhard Zöllner - One of the best experts on this subject based on the ideXlab platform.
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Isolation ofChlamydia trachomatis from the Lower Digestive Tract
Infection, 1993Co-Authors: Bernhard Zöllner, R. Laufs, H. J. Stellbrink, H. Koch, L. Iske, H. -h. Feucht, G. OehlerAbstract:Chlamydia trachomatis was isolated from ten of 188 biopsies (5.3%) obtained from different parts of the Lower Digestive Tract. Patients (mean age 37.0 years) presented with ulcerative proctitis, Crohn's disease, mild colitis or ulcerative colitis. Seven rectal biopsies, two biopsies from the sigmoid flexure and one caecal biopsy were positive for chlamydial isolation whereas all biopsies taken from the colon ascendens, transversum or descendens and from the terminal ileum were negative. We conclude that isolation of C. trachomatis is most effective from rectal and sigmoidal biopsies and is a rare event from other sites of the Lower Digestive Tract. Chlamydia trachomatis wurde aus zehn von 188 Biopsaten (5,3%) isoliert, die aus verschiedenen Regionen des unteren Verdauungstraktes entnommen wurden. Die Diagnosen der Patienten (Durchschnittsalter 37,0 Jahre) waren: ulzerative Proktitis, Morbus Crohn, milde Colitis oder Colitis ulcerosa. Die Chlamydien-Isolierung war positiv bei sieben rektalen Biopsaten, zwei Biopsaten aus dem Sigma und einem Biopsat aus dem Zoekum, während alle Biopsate aus dem Colon ascendens, transversum oder descendens und dem terminalen Ileum negativ waren. Wir schließen daraus, daß die Isolierung von C. trachomatis am effektivsten aus Rektum und Sigma ist und ein seltenes Ereignis aus anderen Regionen des unteren Verdauungstraktes darstellt.
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Isolation of Chlamydia trachomatis from the Lower Digestive Tract
Infection, 1993Co-Authors: Bernhard Zöllner, R. Laufs, H. J. Stellbrink, H. Koch, L. Iske, G. OehlerAbstract:Chlamydia trachomatis was isolated from ten of 188 biopsies (5.3%) obtained from different parts of the Lower Digestive Tract. Patients (mean age 37.0 years) presented with ulcerative proctitis, Crohn's disease, mild colitis or ulcerative colitis. Seven rectal biopsies, two biopsies from the sigmoid flexure and one caecal biopsy were positive for chlamydial isolation whereas all biopsies taken from the colon ascendens, transversum or descendens and from the terminal ileum were negative. We conclude that isolation ofC. trachomatis is most effective from rectal and sigmoidal biopsies and is a rare event from other sites of the Lower Digestive Tract.
James P. Chambers - One of the best experts on this subject based on the ideXlab platform.
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Effects of Bacterial Microflora of the Lower Digestive Tract of Free-Range Waterfowl on Influenza Virus Activation
Applied and environmental microbiology, 2011Co-Authors: Marcus D. King, Bernard P. Arulanandam, Blanca Lupiani, M. Neal Guentzel, Adria A. Bodour, Vinayak Brahmakshatriya, James P. ChambersAbstract:Proteolytic cleavage activation of influenza virus hemagglutinin (HA0) is required for cell entry via receptor-mediated endocytosis. Despite numerous studies describing bacterial protease-mediated influenza A viral activation in mammals, very little is known about the role of intestinal bacterial flora of birds in hemagglutinin cleavage/activation. Therefore, the cloaca of wild waterfowl was examined for (i) representative bacterial types and (ii) their ability to cleave in a "trypsin-like" manner the precursor viral hemagglutinin molecule (HA0). Using radiolabeled HA0, bacterial secretion-mediated trypsin-like conversion of HA0 to HA1 and HA2 peptide products was observed to various degrees in 42 of 44 bacterial isolates suggestive of influenza virus activation in the cloaca of wild waterfowl. However, treatment of uncleaved virus with all bacterial isolates gave rise to substantially reduced emergent virus progeny compared with what was expected. Examination of two isolates exhibiting pronounced trypsin-like conversion of HA0 to HA1 and HA2 peptide products and low infectivity revealed lipase activity to be present. Because influenza virus possesses a complex lipid envelope, the presence of lipid hydrolase activity could in part account for the observed less-than-expected level of viable progeny. A thorough characterization of respective isolate protease HA0 hydrolysis products as well as other resident activities (i.e., lipase) is ongoing such that the role of these respective contributors in virus activation/inactivation can be firmly established.
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Proteolytic bacteria in the Lower Digestive Tract of poultry may affect avian influenza virus pathogenicity
Poultry science, 2009Co-Authors: M. D. King, M. N. Guentzel, Bernard P. Arulanandam, Blanca Lupiani, James P. ChambersAbstract:Proteolytic cleavage of hemagglutinin is required for cell entry by receptor-mediated endocytosis and plays a key role in pathogenicity of the influenza virus. Despite several studies describing relationships between bacterial proteases and influenza A viral activation in mammals, very little is known about the role of the normal bacterial flora of birds on hemagglutinin activation. We examined the indigenous intestinal microflora of 100 mixed-sex, 27-d-old Ross chickens from a commercial poultry facility for protease-secreting bacteria. Protease-secreting bacteria were isolated from 82 of 100 chickens with 50 birds exhibiting 2 or more protease-secreting bacterial species. A total of 20 protease-secreting bacterial species were identified: 17 gram-positive cocci, 2 gram-positive rods, and 1 gram-negative rod. Enterococcus faecalis, Enterococcus gallinarum, and Proteus mirabilis were the most frequently observed protease-secreting bacterial species. The presence of proteolytic bacteria in the intestinal Tract of poultry in this study suggests the possibility of yet-to-be-described role(s) in cleavage of hemagglutinin that may alter the pathogenicity of avian influenza viruses.
A.b.m.m. Van Der Putten - One of the best experts on this subject based on the ideXlab platform.
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The annual yield of diagnostic endoscopy of the Lower Digestive Tract
European journal of internal medicine, 2005Co-Authors: R. J. L. F. Loffeld, A.b.m.m. Van Der PuttenAbstract:AbsTract Background Endoscopy of the colon and rectum has become a standard diagnostic procedure. No data are presented in the literature on the annual yield or on morbidity patterns. A large, cross-sectional, single-center study was done in order to detect the annual yield of endoscopy of the colon and rectum. Methods All consecutive endoscopies performed over an 11-year period were included. All files from a random year were taken in order to collect data on the indication for the endoscopy. A standardized endoscopy report was used. Results Over the 11-year period, 11,550 consecutive endoscopies of the Lower Digestive Tract were performed. Seven hundred and fourteen procedures were excluded because they were done as a direct follow-up after the index procedure. The majority of endoscopies were scheduled as colonoscopy. The most common endoscopic diagnoses made each year remained constant in number. Cancer was diagnosed in 4–6% of cases, inflammation in 9–15%, polyps in 9–16%, and diverticular disease in 21–37%. The percentage of women undergoing the procedure each year ranged from 54% to 59%, that of men from 41% to 46%. Conclusion From this study it can be concluded that the annual yield of endoscopy of the Lower Digestive Tract remains rather constant. No major changes in morbidity are noted.
Liu Hongbiao - One of the best experts on this subject based on the ideXlab platform.
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Clinical value of ~(99m)Tc-RBC imaging in patients with Lower Digestive Tract bleeding and analysis of influencing factors
Zhejiang Medical Journal, 2008Co-Authors: Liu HongbiaoAbstract:Objective To investigate the clinical value of 99mTc-RBC imaging in patients with Lower Digestive Tract bleeding and its influencing factors. Methods 99mTc-RBC imaging was performed in 68 patients with Lower Digestive Tract bleeding. The examine results were analyzed with of sex, age, chronic medical history, stability of blood dynamics, the number of melaena or enterorrhagia episodes in 4 hours before imaging, former bleeding history of gastrointestinal Tract, and therapeutic intervention for bleeding. The results of imaging were also compared with the those of surgery, DSA or colonoscopy. Results There were 30 patients with positive result of 99mTc-RBC imaging with a positive rate of 44.1%. Logistic regression analysis showed that the number of melaena or enterorrhagia episodes and therapeutic intervention for bleeding were significantly different between patients with positive and negative results. The bleeding site was confirmed in 18 of 23 patients with a accurate rate of 78.3%. Conclusion 99mTc-RBC imaging is the effective method to localize Lower Digestive Tract bleeding, and the current episode of bleeding during imaging is the major influencing factor on the positive rate of imaging.
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clinical value of 99m tc rbc imaging in patients with Lower Digestive Tract bleeding and analysis of influencing factors
Zhejiang Medical Journal, 2008Co-Authors: Liu HongbiaoAbstract:Objective To investigate the clinical value of 99mTc-RBC imaging in patients with Lower Digestive Tract bleeding and its influencing factors. Methods 99mTc-RBC imaging was performed in 68 patients with Lower Digestive Tract bleeding. The examine results were analyzed with of sex, age, chronic medical history, stability of blood dynamics, the number of melaena or enterorrhagia episodes in 4 hours before imaging, former bleeding history of gastrointestinal Tract, and therapeutic intervention for bleeding. The results of imaging were also compared with the those of surgery, DSA or colonoscopy. Results There were 30 patients with positive result of 99mTc-RBC imaging with a positive rate of 44.1%. Logistic regression analysis showed that the number of melaena or enterorrhagia episodes and therapeutic intervention for bleeding were significantly different between patients with positive and negative results. The bleeding site was confirmed in 18 of 23 patients with a accurate rate of 78.3%. Conclusion 99mTc-RBC imaging is the effective method to localize Lower Digestive Tract bleeding, and the current episode of bleeding during imaging is the major influencing factor on the positive rate of imaging.
