The Experts below are selected from a list of 681 Experts worldwide ranked by ideXlab platform
P K J P D Wanasundara - One of the best experts on this subject based on the ideXlab platform.
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interference of phenolic compounds in brassica napus brassica rapa and sinapis alba seed extracts with the Lowry Protein Assay
Food Chemistry, 2007Co-Authors: N Lindeboom, P K J P D WanasundaraAbstract:The Protein content of aqueous extracts of Brassica napus, Brassica rapa and Sinapis alba meal was determined by the Lowry and Kjeldahl nitrogen Assays. Phenolic compounds interfered with the Lowry method to different extents based on the lines studied as well as the extraction procedure used. Three ways to correct for this interference were studied; acid precipitation of the Protein before analysis, analyzing in the presence and absence of copper and the binding of free phenolics using non-ionic, porous polystyrene (Amberlite XAD-4). Analysis in presence and absence of copper, and using the difference in absorption at 660 nm between these analyses, proved to be the best way to correct for phenolic interference in the Lowry Assay. Extractability of Cruciferae seed phenolics may be pH dependant thus the contribution of phenolics to the Lowry Protein Assay varies with the pH used for extraction.
N Lindeboom - One of the best experts on this subject based on the ideXlab platform.
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interference of phenolic compounds in brassica napus brassica rapa and sinapis alba seed extracts with the Lowry Protein Assay
Food Chemistry, 2007Co-Authors: N Lindeboom, P K J P D WanasundaraAbstract:The Protein content of aqueous extracts of Brassica napus, Brassica rapa and Sinapis alba meal was determined by the Lowry and Kjeldahl nitrogen Assays. Phenolic compounds interfered with the Lowry method to different extents based on the lines studied as well as the extraction procedure used. Three ways to correct for this interference were studied; acid precipitation of the Protein before analysis, analyzing in the presence and absence of copper and the binding of free phenolics using non-ionic, porous polystyrene (Amberlite XAD-4). Analysis in presence and absence of copper, and using the difference in absorption at 660 nm between these analyses, proved to be the best way to correct for phenolic interference in the Lowry Assay. Extractability of Cruciferae seed phenolics may be pH dependant thus the contribution of phenolics to the Lowry Protein Assay varies with the pH used for extraction.
Rui Hai Liu - One of the best experts on this subject based on the ideXlab platform.
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a modified methylene blue Assay for accurate cell counting
Journal of Functional Foods, 2009Co-Authors: Dana L Felice, Jie Sun, Rui Hai LiuAbstract:Abstract Cell counting is a common technique in cellular and molecular biology research applications, such as cell culture maintenance, cell plating, cell growth and cell doubling time determinations, as well as cell proliferation and cytotoxicity measurements. Many commonly employed cell counting methods exhibit limitations that influence resulting accuracy or versatility. For example, the trypan blue method typically underestimates cell numbers in culture, and the Lowry Protein Assay can be influenced by cell cycle. An urgent need exists for a method of cell counting that is both accurate and versatile. This work intended to explore an adaptation of the methylene blue Assay to overcome the existing limitations of the procedure, enabling application to a broader range of cell densities and various cell culture plates. This new methylene blue Assay was found to be more efficient, accurate and sensitive. A linear relationship ( r 2 > 0.99) was established between cell number and absorbance at 570 nm wavelength when the new methylene blue Assay was applied to three cell lines (HepG2, Caco-2, and MCF-7) plated in a broad range of cell densities (5 × 10 4 to 2.5 × 10 6 ) in four different types of culture plates (6-, 12-, 24-, and 96-well plates). Growth curves were determined using both the trypan blue and methylene blue methods. At each time point in the HepG2 growth curve, the cell count obtained using the trypan blue Assay was statistically significantly lower than that obtained using the methylene blue Assay ( p p
Dana L Felice - One of the best experts on this subject based on the ideXlab platform.
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a modified methylene blue Assay for accurate cell counting
Journal of Functional Foods, 2009Co-Authors: Dana L Felice, Jie Sun, Rui Hai LiuAbstract:Abstract Cell counting is a common technique in cellular and molecular biology research applications, such as cell culture maintenance, cell plating, cell growth and cell doubling time determinations, as well as cell proliferation and cytotoxicity measurements. Many commonly employed cell counting methods exhibit limitations that influence resulting accuracy or versatility. For example, the trypan blue method typically underestimates cell numbers in culture, and the Lowry Protein Assay can be influenced by cell cycle. An urgent need exists for a method of cell counting that is both accurate and versatile. This work intended to explore an adaptation of the methylene blue Assay to overcome the existing limitations of the procedure, enabling application to a broader range of cell densities and various cell culture plates. This new methylene blue Assay was found to be more efficient, accurate and sensitive. A linear relationship ( r 2 > 0.99) was established between cell number and absorbance at 570 nm wavelength when the new methylene blue Assay was applied to three cell lines (HepG2, Caco-2, and MCF-7) plated in a broad range of cell densities (5 × 10 4 to 2.5 × 10 6 ) in four different types of culture plates (6-, 12-, 24-, and 96-well plates). Growth curves were determined using both the trypan blue and methylene blue methods. At each time point in the HepG2 growth curve, the cell count obtained using the trypan blue Assay was statistically significantly lower than that obtained using the methylene blue Assay ( p p
Jie Sun - One of the best experts on this subject based on the ideXlab platform.
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a modified methylene blue Assay for accurate cell counting
Journal of Functional Foods, 2009Co-Authors: Dana L Felice, Jie Sun, Rui Hai LiuAbstract:Abstract Cell counting is a common technique in cellular and molecular biology research applications, such as cell culture maintenance, cell plating, cell growth and cell doubling time determinations, as well as cell proliferation and cytotoxicity measurements. Many commonly employed cell counting methods exhibit limitations that influence resulting accuracy or versatility. For example, the trypan blue method typically underestimates cell numbers in culture, and the Lowry Protein Assay can be influenced by cell cycle. An urgent need exists for a method of cell counting that is both accurate and versatile. This work intended to explore an adaptation of the methylene blue Assay to overcome the existing limitations of the procedure, enabling application to a broader range of cell densities and various cell culture plates. This new methylene blue Assay was found to be more efficient, accurate and sensitive. A linear relationship ( r 2 > 0.99) was established between cell number and absorbance at 570 nm wavelength when the new methylene blue Assay was applied to three cell lines (HepG2, Caco-2, and MCF-7) plated in a broad range of cell densities (5 × 10 4 to 2.5 × 10 6 ) in four different types of culture plates (6-, 12-, 24-, and 96-well plates). Growth curves were determined using both the trypan blue and methylene blue methods. At each time point in the HepG2 growth curve, the cell count obtained using the trypan blue Assay was statistically significantly lower than that obtained using the methylene blue Assay ( p p
