The Experts below are selected from a list of 204 Experts worldwide ranked by ideXlab platform
Yukari Sotohira - One of the best experts on this subject based on the ideXlab platform.
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Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos
Journal of Zoo Biology, 2018Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Mitsuhiko Asakawa, Haruna Okui, Tadashi SanoAbstract:Kangaroo disease (Lumpy Jaw disease; LJD) is a disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with Lumpy Jaw disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with Lumpy Jaw disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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Stress assessment using hair cortisol of kangaroos affected by the Lumpy Jaw disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Chigusa Arai, Mitsuhiko Asakawa, Hideaki HayashiAbstract:The aim of this study was to objectively assess stress of kangaroos affected by Lumpy Jaw disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
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Plasma endotoxin activity in kangaroos with oral necrobacillosis (Lumpy Jaw disease) using an automated handheld testing system
The Journal of veterinary medical science, 2016Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Masakazu Tsuchiya, Toshio Shimamori, Kenji Tsukano, Haruka Sasaki, Yohko Suzuki, Ayano Sato, Hiroshi YokotaAbstract:The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with Lumpy Jaw disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS(TM)) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS(TM) correlated with those using KT (r(2)=0.963, P 0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods.
Mitsuhiko Asakawa - One of the best experts on this subject based on the ideXlab platform.
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Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos
Journal of Zoo Biology, 2018Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Mitsuhiko Asakawa, Haruna Okui, Tadashi SanoAbstract:Kangaroo disease (Lumpy Jaw disease; LJD) is a disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with Lumpy Jaw disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with Lumpy Jaw disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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Stress assessment using hair cortisol of kangaroos affected by the Lumpy Jaw disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Chigusa Arai, Mitsuhiko Asakawa, Hideaki HayashiAbstract:The aim of this study was to objectively assess stress of kangaroos affected by Lumpy Jaw disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
Kazuyuki Suzuki - One of the best experts on this subject based on the ideXlab platform.
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Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos
Journal of Zoo Biology, 2018Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Mitsuhiko Asakawa, Haruna Okui, Tadashi SanoAbstract:Kangaroo disease (Lumpy Jaw disease; LJD) is a disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with Lumpy Jaw disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with Lumpy Jaw disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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Stress assessment using hair cortisol of kangaroos affected by the Lumpy Jaw disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Chigusa Arai, Mitsuhiko Asakawa, Hideaki HayashiAbstract:The aim of this study was to objectively assess stress of kangaroos affected by Lumpy Jaw disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
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Plasma endotoxin activity in kangaroos with oral necrobacillosis (Lumpy Jaw disease) using an automated handheld testing system
The Journal of veterinary medical science, 2016Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Masakazu Tsuchiya, Toshio Shimamori, Kenji Tsukano, Haruka Sasaki, Yohko Suzuki, Ayano Sato, Hiroshi YokotaAbstract:The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with Lumpy Jaw disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS(TM)) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS(TM) correlated with those using KT (r(2)=0.963, P 0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods.
Gale B. Hill - One of the best experts on this subject based on the ideXlab platform.
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Eubacterium nodatum mimics Actinomyces in intrauterine device-associated infections and other settings within the female genital tract.
Obstetrics and gynecology, 1992Co-Authors: Gale B. HillAbstract:Eubacterium nodatum is an obligately anaerobic, gram-positive, branching rod that markedly resembles Actinomyces, particularly Actinomyces israelii, in its cellular and colonial characteristics. Its isolation from the female genital tract was examined for a study period in which use of intrauterine contraceptive devices (IUDs) was common, and additional characteristics of the organism were investigated. Fifteen genital isolates of E nodatum were all associated with the presence of a foreign body, usually an IUD (12 patients). Six of these 12 patients had presented with clinically severe pelvic inflammatory disease. The remaining six had signs and symptoms related to IUD use and/or had a report of probable Actinomyces (five patients) by a Papanicolaou smear, demonstrating that E nodatum can be mistaken for Actinomyces in a Papanicolaou-stained smear. The three other patients had different types of foreign bodies. The frequency of isolation from cultures associated with IUD use during the study period was five (6.4%) of 78 for Actinomyces versus the 12 (15.4%) of 78 for E nodatum. In vitro-prepared E nodatum was not demonstrated to cross-react with A israelii or A naeslundii antisera. Both E nodatum and A israelii were shown to adhere in vitro to an inanimate object, indicating their propensities to colonize a foreign body. The present data, with the previous reports of isolation of E nodatum from cases of Lumpy Jaw and severe periodontitis, suggest that it is an opportunistic pathogen very much like A israelii.
C Regamey - One of the best experts on this subject based on the ideXlab platform.
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disseminated infection due to actinomyces meyeri case report and review
Clinical Infectious Diseases, 1996Co-Authors: C Apotheloz, C RegameyAbstract:Actinomyces meyeri is rarely isolated in cases of actinomycosis. We present a case of disseminated actinomycosis due to A. meyeri; the patient had an abscess of the lung, osteomyelitis of the tibia, and multiple skin abscesses. Cure was achieved with surgical debridement and administration of intravenous penicillin, followed by oral penicillin, for 1 year. A concomitant gram-negative bacillus, Actinobacillus actinomycetemcomitans, was also isolated. Review of the literature revealed only 26 well-documented cases of infection with A. meyeri. Male adults are mainly affected, and alcoholism is frequently the underlying condition in these patients. Associated bacteria were isolated in two thirds of these cases. In contrast to other species of Actinomyces, A. meyeri often causes pulmonary infection and shows a tendency for hematogenous dissemination. Even though multiple organs are involved, the outcome for these patients is excellent when penicillin is administered for several months and surgical procedures are performed when necessary. The term actinomycete, which is of Greek origin, means ray fungus; however, actinomycosis is due to a gram-positive anaerobic bacterium. Bollinger [1] described the microorgan ism in 1877 as the causative agent of Lumpy Jaw in cattle and named it Actinomyces bovis [1]. In 1891, Wolff and Israel [2] grew a similar microorganism from a pulmonary abscess and named it Actinomyces israelii [2]. Although most cases of hu man actinomycosis are due to A. israelii, less common agents are Actinomyces naeslundii, Actinomyces viscosus, Actinomy ces odontolyticus, Actinomycesmeyeri, and Propionibacterium propionicum. A. meyeri was first isolated from a lung abscess in 1911 by Meyer, who described it as a Streptothrix [3]. The microorgan ism was then reclassified as Actinobacterium meyeri in 1938 [4]; it was finally classified in the order Actinomycetales in 1977 [5]. This rare actinomycete has been recovered from a minority of patients, and the spectrum of infections it causes is not well known, although it shares all the microbiological characteristics of the organisms in this order. We describe a case of disseminated actinomycosis due to A. meyeri.
