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Cortés Avendaño, Paola Marlene - One of the best experts on this subject based on the ideXlab platform.
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Caracterización funcional de ecotipos de Tarwi (Lupinus mutabilis sweet) para su inclusión en una bebida probiótica
'Baishideng Publishing Group Inc.', 2020Co-Authors: Cortés Avendaño, Paola MarleneAbstract:El objetivo del trabajo de investigación fue realizar la caracterización funcional de ecotipos de tarwi (Lupinus mutabilis Sweet) para su inclusión en una bebida probiótica. En la primera etapa se realizó la caracterización física y química de diez ecotipos de lupino sin desamargar y desamargados, eligiendo al mejor ecotipo para la elaboración de la bebida probiótica. En la segunda etapa, se optimizó la formulación de la bebida probiótica en función a la viabilidad de los probióticos y el pH. En la tercera etapa, se caracterizó y evalúo la estabilidad de la bebida probiótica óptima. En las semillas sin desamargar se identificaron 8 alcaloides, principalmente lupanina (2.5-5.2 g/100g) y esparteína (0.2-0.9g /100g), que constituyeron el 80% del total de alcaloides. En las semillas desamargadas se identificaron sólo lupanina (0.0012 g/100g) y esparteína (0.0014 g/100g). El contenido de isoflavonas y flavonas en las semillas sin desamargar fue 185.3 mg/100g y 40 mg/100g, respectivamente y en las semillas desamargadas 148 mg de genisteína /100 g y 17 mg de apigenina /100g, respectivamente. El ecotipo seleccionado para la elaboración de la bebida fue Altagracia por su contenido de proteínas, alcaloides, isoflavonas y flavonas, y el probiótico elegido fue Lactobacillus plantarum 299v, en función a la reducción de pH. La bebida probiótica óptima (8% de azúcar y 31.5% de lupino: agua, 2% de inulina, 0.8% de CMC, 1% de glucosa) fermentada durante 12 h con L. plantarum 299v mostró una tasa de crecimiento de 30.63% (13 x 108 UFC/ml) y buena aceptabilidad general (5.4 ± 2.4). Durante el almacenamiento, disminuyó la viabilidad del probiótico (57 x 107 UFC/ml), la viscosidad (534.4 cP) y el pH (3.85) y se incrementó de la acidez titulable. Las semillas de lupino, por sus características nutricionales, es una buena matriz vegetal para el crecimiento de las bacterias probióticas.The aim of the research work was to perform the functional characterization of tarwi (Lupinus mutabilis Sweet) ecotypes for inclusion in a probiotic drink. In the first stage, the physical and chemical characterization of ten lupine ecotypes was carried out without debittering and debittering. Then the ecotype was selected for the preparation of the probiotic drink. In the second stage, the formulation of the probiotic drink was optimized based on the viability of the probiotics and the pH. In the third stage, the optimal probiotic drink was characterized and stability evaluated. In the seeds without debittering, 8 alkaloids were identified, mainly Lupanine (2.5-5.2 g / 100 g) and sparteine (0.2-0.9 g / 100 g), which constituted 80% of the total alkaloids. Only Lupanine (0.0012 g / 100 g) and sparteine (0.0014 g / 100 g) were identified in the debittered seeds. The content of isoflavones and flavones were: in the seeds without debittering 185.3 mg / 100 g and 40 mg / 100 g, respectively and in the debittering seeds 148 mg of genistein / 100 g and 17 mg of apigenin / 100 g, respectively. The ecotype selected for the preparation of the drink was Altagracia due to its content of proteins, alkaloids, isoflavones and flavones, and the probiotic chosen was Lactobacillus plantarum 299v, based on the reduction in pH. The optimal probiotic drink (8% sugar and 31.5% lupine: water, 2% inulin, 0.8% CMC, 1% glucose) fermented for 12 h with Lactobacillus plantarum 299v showed a growth rate of 30.63% (13 x 108 CFU / ml) and good general acceptability (5.4 ± 2.4). During storage, the viability of the probiotic (57 x 107 CFU / ml), the viscosity (534.4 cP) and the pH (3.85) decreased and the titratable acidity increased. Lupine seeds, due to their nutritional characteristics, are a good plant matrix for the growth of probiotic bacteria
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Caracterización funcional de ecotipos de Tarwi (Lupinus mutabilis sweet) para su inclusión en una bebida probiótica
'Baishideng Publishing Group Inc.', 2020Co-Authors: Cortés Avendaño, Paola MarleneAbstract:Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Ciencia de AlimentosEl objetivo del trabajo de investigación fue realizar la caracterización funcional de ecotipos de tarwi (Lupinus mutabilis Sweet) para su inclusión en una bebida probiótica. En la primera etapa se realizó la caracterización física y química de diez ecotipos de lupino sin desamargar y desamargados, eligiendo al mejor ecotipo para la elaboración de la bebida probiótica. En la segunda etapa, se optimizó la formulación de la bebida probiótica en función a la viabilidad de los probióticos y el pH. En la tercera etapa, se caracterizó y evalúo la estabilidad de la bebida probiótica óptima. En las semillas sin desamargar se identificaron 8 alcaloides, principalmente lupanina (2.5-5.2 g/100g) y esparteína (0.2-0.9g /100g), que constituyeron el 80% del total de alcaloides. En las semillas desamargadas se identificaron sólo lupanina (0.0012 g/100g) y esparteína (0.0014 g/100g). El contenido de isoflavonas y flavonas en las semillas sin desamargar fue 185.3 mg/100g y 40 mg/100g, respectivamente y en las semillas desamargadas 148 mg de genisteína /100 g y 17 mg de apigenina /100g, respectivamente. El ecotipo seleccionado para la elaboración de la bebida fue Altagracia por su contenido de proteínas, alcaloides, isoflavonas y flavonas, y el probiótico elegido fue Lactobacillus plantarum 299v, en función a la reducción de pH. La bebida probiótica óptima (8% de azúcar y 31.5% de lupino: agua, 2% de inulina, 0.8% de CMC, 1% de glucosa) fermentada durante 12 h con L. plantarum 299v mostró una tasa de crecimiento de 30.63% (13 x 108 UFC/ml) y buena aceptabilidad general (5.4 ± 2.4). Durante el almacenamiento, disminuyó la viabilidad del probiótico (57 x 107 UFC/ml), la viscosidad (534.4 cP) y el pH (3.85) y se incrementó de la acidez titulable. Las semillas de lupino, por sus características nutricionales, es una buena matriz vegetal para el crecimiento de las bacterias probióticas.The aim of the research work was to perform the functional characterization of tarwi (Lupinus mutabilis Sweet) ecotypes for inclusion in a probiotic drink. In the first stage, the physical and chemical characterization of ten lupine ecotypes was carried out without debittering and debittering. Then the ecotype was selected for the preparation of the probiotic drink. In the second stage, the formulation of the probiotic drink was optimized based on the viability of the probiotics and the pH. In the third stage, the optimal probiotic drink was characterized and stability evaluated. In the seeds without debittering, 8 alkaloids were identified, mainly Lupanine (2.5-5.2 g / 100 g) and sparteine (0.2-0.9 g / 100 g), which constituted 80% of the total alkaloids. Only Lupanine (0.0012 g / 100 g) and sparteine (0.0014 g / 100 g) were identified in the debittered seeds. The content of isoflavones and flavones were: in the seeds without debittering 185.3 mg / 100 g and 40 mg / 100 g, respectively and in the debittering seeds 148 mg of genistein / 100 g and 17 mg of apigenin / 100 g, respectively. The ecotype selected for the preparation of the drink was Altagracia due to its content of proteins, alkaloids, isoflavones and flavones, and the probiotic chosen was Lactobacillus plantarum 299v, based on the reduction in pH. The optimal probiotic drink (8% sugar and 31.5% lupine: water, 2% inulin, 0.8% CMC, 1% glucose) fermented for 12 h with Lactobacillus plantarum 299v showed a growth rate of 30.63% (13 x 108 CFU / ml) and good general acceptability (5.4 ± 2.4). During storage, the viability of the probiotic (57 x 107 CFU / ml), the viscosity (534.4 cP) and the pH (3.85) decreased and the titratable acidity increased. Lupine seeds, due to their nutritional characteristics, are a good plant matrix for the growth of probiotic bacteria
Edison Serrano - One of the best experts on this subject based on the ideXlab platform.
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responses in rainbow trout oncorhynchus mykiss to increasing dietary doses of lupinine the main quinolizidine alkaloid found in yellow lupins lupinus luteus
Aquaculture, 2011Co-Authors: Edison Serrano, Trond Storebakken, Michael H Penn, Margareth Overland, Jon Ovrum Hansen, Liv Torunn MydlandAbstract:Abstract This experiment investigated the effect of increasing dietary doses of lupinine, the main quinolizidine alkaloid in Lupinus luteus , on feed intake, growth performance, tissue histology and nutritional composition of rainbow trout ( Oncorhynchus mykiss ). Duplicate groups of rainbow trout (initial body weight of 330 g) were fed extruded fish meal based diets containing 0, 50, 75, 100, 250, 500, 1000 and 5000 mg lupinine kg −1 for 60 days. Feed intake and growth were reduced in response to dietary lupinine, best fit by quadratic regression. Based on these results, the practical tolerance level of lupinine, with regard to growth and feed intake, was ≤ 100 mg kg −1 feed. Carcass composition did not vary among treatments. Despite a depletion of glycogen and lipid stores in the hepatocytes, lupinine did not induce any morphological changes in spleen, kidney, heart or intestinal tissues. These results indicate that the lupinine possesses a strong anti-palatability effect, but does not appear to pose short-term health risks for rainbow trout.
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responses in rainbow trout oncorhynchus mykiss to increasing dietary dose of lupinine alkaloid
Lupins for health and wealth. Proceedings of the 12th International Lupin Conference Fremantle Western Australia 14-18 September 2008, 2008Co-Authors: Edison Serrano, Trond Storebakken, Michael H Penn, Jon Ovrum Hansen, Thor Landsverk, Liv Torunn MydlandAbstract:Yellow lupin ( Lupinus luteus ) is a promising source of protein in feeds for carnivorous fish. However, the high content of alkaloids may limit its potential for use. Lupinine is the main quinolizidine alkaloid in several varieties of Lupinus luteus . It has been reported to be highly toxic for bacteria and invertebrates, however no information is available about the allelopathic effects of lupinine on vertebrates. This study investigated the effect of increasing dietary doses of lupinine on feed intake, growth performance, tissue histology and nutritional composition of rainbow trout. Duplicate groups of rainbow trout (initial body weight of 0.3 kg) were fed extruded fish meal based diets containing 0, 50, 75, 100, 250, 500, 1000 and 5000 mg lupinine/kg for 60 days. Results from this study show that feed intake and growth were linearly reduced in response to dietary lupinine. Based on results obtained by additional analysis of variance, the practical tolerance levels of lupinine with regard to growth and feed intake was up to 100 mg/kg feed. Carcass composition did not vary among treatment. Besides a depletion of glycogen and lipid stores in the hepatocytes, the dietary inclusion of lupinine did not cause any morphological changes in kidney, heart or intestinal tissue. These results indicate that the lupinine alkaloid possesses a strong anti-palatability effect, but does not pose an adverse short-term risk to the health of rainbow trout.
Kazuki Saito - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and partial molecular characterization of bitter and sweet forms of Lupinus angustifolius, an experimental model for study of molecular regulation of quinolizidine alkaloid biosynthesis.
Chemical & pharmaceutical bulletin, 2000Co-Authors: Masami Yokota Hirai, Hedeyuki Suzuki, Mami Yamazaki, Kazuki SaitoAbstract:The bitter and sweet forms of a plant species differing with alkaloid contents may provide a model system for investigation of alkaloid biosynthesis at a molecular level. The pattern and concentration of quinolizidine alkaloids were determined by capillary GC-MS in bitter and sweet plants of Lupinus angustifolius. Bitter plant contained Lupanine, 13alpha-hydroxyLupanine, angustifoline, alpha-isoLupanine, tetrahydrorhombifoline, and ester-derivatives of 13alpha-hydroxyLupanine. In contrast, no alkaloid was detected in sweet plant. The enzymatic activity of acyltransferase for formation of 13alpha-tigloyloxyLupanine was similar or even higher in the cell-free extracts of sweet plant than that in bitter plant. These results suggest that the biosynthetic step(s) of ring closure forming the initial cyclic alkaloid, Lupanine, from cadaverine is presumably blocked in sweet plant, and that the later steps for modification of the cyclized alkaloids are not altered. We hypothesized that the gene(s) encoding enzyme(s) for ring-closure step might be repressed in sweet plant, and that the expression might take place only in bitter plant. To isolate the genes specifically expressed in bitter plant, cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was carried out. However, no bitter-specific gene was isolated, suggesting that alkaloid biosynthesis in sweet plant may be down-regulated at a post-transcriptional level.
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isolation and enzymatic synthesis of an ester alkaloid 3β hydroxy 13α tigloyloxyLupanine from cytisus scoparius
Phytochemistry, 1994Co-Authors: Kazuki Saito, Yoshiaki Yamashita, Hideyuki Suzuki, Isamu MurakoshiAbstract:Abstract A new ester alkaloid, (−)-3β-hydroxy-13α-tigloyloxyLupanine was isolated from the seedlings of Cytisus scoparius. Six known alkaloids, (−)-3β,13α-dihydroxyLupanine, (+)-13α-tigloyloxyLupanine, (+)-13α-hydroxy-Lupanine, (+)-Lupanine, (−)-sparteine and (−)-17-oxosparteine were also identified by co-TLC, co-HPLC and GCMS with authentic samples. The structure of the new alkaloid was determined by spectroscopic methods. The new acyltransferase activity, tigloyl-CoA: 3β,13α-dihydroxyLupanine 13-O-tigloyltransferase (DHLTase) [EC 2.3.1.-], for the formation of this alkaloid was detected in the cell-free extracts of 15-day-old seedlings of C. scoparius.
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biogenetic implication of lupin alkaloid biosynthesis in bitter and sweet forms of lupinus luteus and l albus
Phytochemistry, 1993Co-Authors: Kazuki Saito, Hideyuki Suzuki, Youichi Koike, Isamu MurakoshiAbstract:Abstract The concentrations of lupin alkaloids, l -lysine and cadaverine were determined by a capillary GC-MS and an amino acid analyser in bitter and sweet plants of Lupinus luteus and L. albus. The contents of total crude alkaloids were ca four-fold higher in bitter plants than those in sweet plants; by contrast, the alkaloid patterns were similar in bitter and sweet plants. However, the cellular concentrations of l -lysine and cadaverine, a precursor amino acid and a decarboxylated intermediate for biosynthesis of the alkaloids, did not differ markedly between bitter and sweet plants. The enzymatic activities of acyltransferases for formation of (-)-p-coumaroyllupinine and (+)- 13α-tigloyloxyLupanine were also the same in the cell-free extracts of bitter and sweet plants. These results suggest that the biosynthetic steps of ring closure forming initial cyclic alkaloids such as (-)-lupinine and (+)-Lupanine from cadaverine are blocked in sweet plants, but the steps prior to cadaverine and the later steps for modification of the cyclized alkaloids are not altere
Isamu Murakoshi - One of the best experts on this subject based on the ideXlab platform.
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isolation and enzymatic synthesis of an ester alkaloid 3β hydroxy 13α tigloyloxyLupanine from cytisus scoparius
Phytochemistry, 1994Co-Authors: Kazuki Saito, Yoshiaki Yamashita, Hideyuki Suzuki, Isamu MurakoshiAbstract:Abstract A new ester alkaloid, (−)-3β-hydroxy-13α-tigloyloxyLupanine was isolated from the seedlings of Cytisus scoparius. Six known alkaloids, (−)-3β,13α-dihydroxyLupanine, (+)-13α-tigloyloxyLupanine, (+)-13α-hydroxy-Lupanine, (+)-Lupanine, (−)-sparteine and (−)-17-oxosparteine were also identified by co-TLC, co-HPLC and GCMS with authentic samples. The structure of the new alkaloid was determined by spectroscopic methods. The new acyltransferase activity, tigloyl-CoA: 3β,13α-dihydroxyLupanine 13-O-tigloyltransferase (DHLTase) [EC 2.3.1.-], for the formation of this alkaloid was detected in the cell-free extracts of 15-day-old seedlings of C. scoparius.
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biogenetic implication of lupin alkaloid biosynthesis in bitter and sweet forms of lupinus luteus and l albus
Phytochemistry, 1993Co-Authors: Kazuki Saito, Hideyuki Suzuki, Youichi Koike, Isamu MurakoshiAbstract:Abstract The concentrations of lupin alkaloids, l -lysine and cadaverine were determined by a capillary GC-MS and an amino acid analyser in bitter and sweet plants of Lupinus luteus and L. albus. The contents of total crude alkaloids were ca four-fold higher in bitter plants than those in sweet plants; by contrast, the alkaloid patterns were similar in bitter and sweet plants. However, the cellular concentrations of l -lysine and cadaverine, a precursor amino acid and a decarboxylated intermediate for biosynthesis of the alkaloids, did not differ markedly between bitter and sweet plants. The enzymatic activities of acyltransferases for formation of (-)-p-coumaroyllupinine and (+)- 13α-tigloyloxyLupanine were also the same in the cell-free extracts of bitter and sweet plants. These results suggest that the biosynthetic steps of ring closure forming initial cyclic alkaloids such as (-)-lupinine and (+)-Lupanine from cadaverine are blocked in sweet plants, but the steps prior to cadaverine and the later steps for modification of the cyclized alkaloids are not altere
Liv Torunn Mydland - One of the best experts on this subject based on the ideXlab platform.
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responses in rainbow trout oncorhynchus mykiss to increasing dietary doses of lupinine the main quinolizidine alkaloid found in yellow lupins lupinus luteus
Aquaculture, 2011Co-Authors: Edison Serrano, Trond Storebakken, Michael H Penn, Margareth Overland, Jon Ovrum Hansen, Liv Torunn MydlandAbstract:Abstract This experiment investigated the effect of increasing dietary doses of lupinine, the main quinolizidine alkaloid in Lupinus luteus , on feed intake, growth performance, tissue histology and nutritional composition of rainbow trout ( Oncorhynchus mykiss ). Duplicate groups of rainbow trout (initial body weight of 330 g) were fed extruded fish meal based diets containing 0, 50, 75, 100, 250, 500, 1000 and 5000 mg lupinine kg −1 for 60 days. Feed intake and growth were reduced in response to dietary lupinine, best fit by quadratic regression. Based on these results, the practical tolerance level of lupinine, with regard to growth and feed intake, was ≤ 100 mg kg −1 feed. Carcass composition did not vary among treatments. Despite a depletion of glycogen and lipid stores in the hepatocytes, lupinine did not induce any morphological changes in spleen, kidney, heart or intestinal tissues. These results indicate that the lupinine possesses a strong anti-palatability effect, but does not appear to pose short-term health risks for rainbow trout.
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responses in rainbow trout oncorhynchus mykiss to increasing dietary dose of lupinine alkaloid
Lupins for health and wealth. Proceedings of the 12th International Lupin Conference Fremantle Western Australia 14-18 September 2008, 2008Co-Authors: Edison Serrano, Trond Storebakken, Michael H Penn, Jon Ovrum Hansen, Thor Landsverk, Liv Torunn MydlandAbstract:Yellow lupin ( Lupinus luteus ) is a promising source of protein in feeds for carnivorous fish. However, the high content of alkaloids may limit its potential for use. Lupinine is the main quinolizidine alkaloid in several varieties of Lupinus luteus . It has been reported to be highly toxic for bacteria and invertebrates, however no information is available about the allelopathic effects of lupinine on vertebrates. This study investigated the effect of increasing dietary doses of lupinine on feed intake, growth performance, tissue histology and nutritional composition of rainbow trout. Duplicate groups of rainbow trout (initial body weight of 0.3 kg) were fed extruded fish meal based diets containing 0, 50, 75, 100, 250, 500, 1000 and 5000 mg lupinine/kg for 60 days. Results from this study show that feed intake and growth were linearly reduced in response to dietary lupinine. Based on results obtained by additional analysis of variance, the practical tolerance levels of lupinine with regard to growth and feed intake was up to 100 mg/kg feed. Carcass composition did not vary among treatment. Besides a depletion of glycogen and lipid stores in the hepatocytes, the dietary inclusion of lupinine did not cause any morphological changes in kidney, heart or intestinal tissue. These results indicate that the lupinine alkaloid possesses a strong anti-palatability effect, but does not pose an adverse short-term risk to the health of rainbow trout.
