The Experts below are selected from a list of 16542 Experts worldwide ranked by ideXlab platform
Jo Shu Chang - One of the best experts on this subject based on the ideXlab platform.
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a highly efficient two stage cultivation strategy for Lutein production using heterotrophic culture of chlorella sorokiniana mb 1 m12
Bioresource Technology, 2018Co-Authors: Chun Yen Chen, Duu-jong Lee, Dillirani Nagarajan, Chien Hsiang Chang, Jo Shu ChangAbstract:Abstract A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce Lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal Lutein content (3.67 mg Lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the Lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, Lutein content and Lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high Lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic Lutein production in commercial scale.
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Lutein production from biomass marigold flowers versus microalgae
Bioresource Technology, 2015Co-Authors: Jian Hao Lin, Duu-jong Lee, Jo Shu ChangAbstract:Microalgae have faster growth rates and more free Lutein than marigold flowers, the current source of Lutein. However, no commercial Lutein production uses microalgae. This review compares Lutein content, cultivation, harvesting, cell disruption, and extraction stages of Lutein production using marigold flowers and those using microalgae as feedstock. The Lutein production rate of microalgae is 3-6 times higher than that of marigold flowers. To produce 1 kg of pure Lutein, marigolds need more land and water, but require less nutrients (N, P, K) and less energy than microalgae. Since Lutein is tightly bound in microalgae and microalgae are small, cell disruption and subsequent extraction stages consume a considerable amount of energy. Research and development of affordable Lutein production from microalgae are discussed.
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effects of nitrogen source availability and bioreactor operating strategies on Lutein production with scenedesmus obliquus fsp 3
Bioresource Technology, 2015Co-Authors: Youping Xie, Duu-jong Lee, Ming Chang Chan, Chun Yen Chen, Chiehchen Huang, Chen Chun Liu, Jo Shu ChangAbstract:In this study, the effects of the type and concentration of nitrogen sources on the cell growth and Lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest Lutein content (4.61 mg/g) and Lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the Lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and Lutein productivity (6.01 mg/L/day). This performance is better than most related studies.
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Lutein in specific marigold flowers and microalgae
Journal of the Taiwan Institute of Chemical Engineers, 2015Co-Authors: Jian Hao Lin, Duu-jong Lee, Jo Shu ChangAbstract:Abstract Lutein is currently produced from marigold flowers. Microalgae can grow fast with high fixation rate of CO2, which have been proposed an alternative Lutein source. This communication determined the contents of free Lutein and Lutein ester of two marigold flowers (Tagetes erecta and Tagetes patula) and two microalgae samples (Chlorella pyrenoidosa and Scenedesmus obliquus) using the same Lutein extraction and identification protocol. The tested T. erecta has low Lutein content. The T. patula has high content of Lutein ester. Conversely, the tested C. pyrenoidosa and S. obliquus exhibit high contents of free Lutein and chlorophyll and minimal quantity of Lutein ester. Microalgae can be used instead of marigold flowers for Lutein production if rapid production of free Lutein products is required.
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enhancing Lutein productivity of an indigenous microalga scenedesmus obliquus fsp 3 using light related strategies
Bioresource Technology, 2014Co-Authors: Ming Chang Chan, Duu-jong Lee, Chun Yen Chen, Chen Chun Liu, Wen Lung Lee, Jo Shu ChangAbstract:Lutein, one of the main photosynthetic pigments, is a promising natural product with both nutritional and pharmaceutical applications. In this study, light-related strategies were applied to enhance the cell growth and Lutein production of a Lutein-rich microalga Scenedesmus obliquus FSP-3. The results demonstrate that using white LED resulted in better Lutein production efficiency when compared to the other three monochromatic LEDs (red, blue, and green). The Lutein productivity of S. obliquus FSP-3 was further improved by adjusting the type of light source and light intensity. The optimal Lutein productivity of 4.08 mg/L/d was obtained when using a TL5 fluorescent lamp at a light intensity of 300 μmol/m(2)/s, and this performance is better than that reported in most related studies. Moreover, the time-course profile of Lutein accumulation in the microalga shows that the maximal Lutein content and productivity were obtained at the onset of nitrogen depletion.
Duu-jong Lee - One of the best experts on this subject based on the ideXlab platform.
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a highly efficient two stage cultivation strategy for Lutein production using heterotrophic culture of chlorella sorokiniana mb 1 m12
Bioresource Technology, 2018Co-Authors: Chun Yen Chen, Duu-jong Lee, Dillirani Nagarajan, Chien Hsiang Chang, Jo Shu ChangAbstract:Abstract A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce Lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal Lutein content (3.67 mg Lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the Lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, Lutein content and Lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high Lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic Lutein production in commercial scale.
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Lutein production from biomass marigold flowers versus microalgae
Bioresource Technology, 2015Co-Authors: Jian Hao Lin, Duu-jong Lee, Jo Shu ChangAbstract:Microalgae have faster growth rates and more free Lutein than marigold flowers, the current source of Lutein. However, no commercial Lutein production uses microalgae. This review compares Lutein content, cultivation, harvesting, cell disruption, and extraction stages of Lutein production using marigold flowers and those using microalgae as feedstock. The Lutein production rate of microalgae is 3-6 times higher than that of marigold flowers. To produce 1 kg of pure Lutein, marigolds need more land and water, but require less nutrients (N, P, K) and less energy than microalgae. Since Lutein is tightly bound in microalgae and microalgae are small, cell disruption and subsequent extraction stages consume a considerable amount of energy. Research and development of affordable Lutein production from microalgae are discussed.
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effects of nitrogen source availability and bioreactor operating strategies on Lutein production with scenedesmus obliquus fsp 3
Bioresource Technology, 2015Co-Authors: Youping Xie, Duu-jong Lee, Ming Chang Chan, Chun Yen Chen, Chiehchen Huang, Chen Chun Liu, Jo Shu ChangAbstract:In this study, the effects of the type and concentration of nitrogen sources on the cell growth and Lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest Lutein content (4.61 mg/g) and Lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the Lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and Lutein productivity (6.01 mg/L/day). This performance is better than most related studies.
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Lutein in specific marigold flowers and microalgae
Journal of the Taiwan Institute of Chemical Engineers, 2015Co-Authors: Jian Hao Lin, Duu-jong Lee, Jo Shu ChangAbstract:Abstract Lutein is currently produced from marigold flowers. Microalgae can grow fast with high fixation rate of CO2, which have been proposed an alternative Lutein source. This communication determined the contents of free Lutein and Lutein ester of two marigold flowers (Tagetes erecta and Tagetes patula) and two microalgae samples (Chlorella pyrenoidosa and Scenedesmus obliquus) using the same Lutein extraction and identification protocol. The tested T. erecta has low Lutein content. The T. patula has high content of Lutein ester. Conversely, the tested C. pyrenoidosa and S. obliquus exhibit high contents of free Lutein and chlorophyll and minimal quantity of Lutein ester. Microalgae can be used instead of marigold flowers for Lutein production if rapid production of free Lutein products is required.
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enhancing Lutein productivity of an indigenous microalga scenedesmus obliquus fsp 3 using light related strategies
Bioresource Technology, 2014Co-Authors: Ming Chang Chan, Duu-jong Lee, Chun Yen Chen, Chen Chun Liu, Wen Lung Lee, Jo Shu ChangAbstract:Lutein, one of the main photosynthetic pigments, is a promising natural product with both nutritional and pharmaceutical applications. In this study, light-related strategies were applied to enhance the cell growth and Lutein production of a Lutein-rich microalga Scenedesmus obliquus FSP-3. The results demonstrate that using white LED resulted in better Lutein production efficiency when compared to the other three monochromatic LEDs (red, blue, and green). The Lutein productivity of S. obliquus FSP-3 was further improved by adjusting the type of light source and light intensity. The optimal Lutein productivity of 4.08 mg/L/d was obtained when using a TL5 fluorescent lamp at a light intensity of 300 μmol/m(2)/s, and this performance is better than that reported in most related studies. Moreover, the time-course profile of Lutein accumulation in the microalga shows that the maximal Lutein content and productivity were obtained at the onset of nitrogen depletion.
Elizabeth J Johnson - One of the best experts on this subject based on the ideXlab platform.
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Lutein and Brain Function
Foods (Basel Switzerland), 2015Co-Authors: John W. Erdman, Elizabeth J Johnson, Joshua W. Smith, Matthew J. Kuchan, Emily S. Mohn, Stanislav S. Rubakhin, Lin Wang, Jonathan V. Sweedler, Martha NeuringerAbstract:Lutein is one of the most prevalent carotenoids in nature and in the human diet. Together with zeaxanthin, it is highly concentrated as macular pigment in the foveal retina of primates, attenuating blue light exposure, providing protection from photo-oxidation and enhancing visual performance. Recently, interest in Lutein has expanded beyond the retina to its possible contributions to brain development and function. Only primates accumulate Lutein within the brain, but little is known about its distribution or physiological role. Our team has begun to utilize the rhesus macaque (Macaca mulatta) model to study the uptake and bio-localization of Lutein in the brain. Our overall goal has been to assess the association of Lutein localization with brain function. In this review, we will first cover the evolution of the non-human primate model for Lutein and brain studies, discuss prior association studies of Lutein with retina and brain function, and review approaches that can be used to localize brain Lutein. We also describe our approach to the biosynthesis of 13C-Lutein, which will allow investigation of Lutein flux, localization, metabolism and pharmacokinetics. Lastly, we describe potential future research opportunities.
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exploratory metabolomic analyses reveal compounds correlated with Lutein concentration in frontal cortex hippocampus and occipital cortex of human infant brain
PLOS ONE, 2015Co-Authors: Jacqueline C Liebleinboff, Elizabeth J Johnson, Adam D Kennedy, Chronsi Lai, Matthew J. KuchanAbstract:Lutein is a dietary carotenoid well known for its role as an antioxidant in the macula, and recent reports implicate a role for Lutein in cognitive function. Lutein is the dominant carotenoid in both pediatric and geriatric brain tissue. In addition, cognitive function in older adults correlated with macular and postmortem brain Lutein concentrations. Furthermore, Lutein was found to preferentially accumulate in the infant brain in comparison to other carotenoids that are predominant in diet. While Lutein is consistently related to cognitive function, the mechanisms by which Lutein may influence cognition are not clear. In an effort to identify potential mechanisms through which Lutein might influence neurodevelopment, an exploratory study relating metabolite signatures and Lutein was completed. Post-mortem metabolomic analyses were performed on human infant brain tissues in three regions important for learning and memory: the frontal cortex, hippocampus, and occipital cortex. Metabolomic profiles were compared to Lutein concentration, and correlations were identified and reported here. A total of 1276 correlations were carried out across all brain regions. Of 427 metabolites analyzed, 257 were metabolites of known identity. Unidentified metabolite correlations (510) were excluded. In addition, moderate correlations with xenobiotic relationships (2) or those driven by single outliers (3) were excluded from further study. Lutein concentrations correlated with lipid pathway metabolites, energy pathway metabolites, brain osmolytes, amino acid neurotransmitters, and the antioxidant homocarnosine. These correlations were often brain region-specific. Revealing relationships between Lutein and metabolic pathways may help identify potential candidates on which to complete further analyses and may shed light on important roles of Lutein in the human brain during development.
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Role of Lutein and zeaxanthin in visual and cognitive function throughout the lifespan.
Nutrition reviews, 2014Co-Authors: Elizabeth J JohnsonAbstract:The relationship between Lutein and zeaxanthin and visual and cognitive health throughout the lifespan is compelling. There is a variety of evidence to support a role for Lutein and zeaxanthin in vision. Lutein's role in cognition has only recently been considered. Lutein and its isomer, zeaxanthin, are taken up selectively into eye tissue. Lutein is the predominant carotenoid in human brain tissue. Lutein and zeaxanthin in neural tissue may have biological effects that include antioxidation, anti-inflammation, and structural actions. In addition, Lutein and zeaxanthin may be protective against eye disease because they absorb damaging blue light that enters the eye. In pediatric brains, the relative contribution of Lutein to the total carotenoids is twice that found in adults, accounting for more than half the concentration of total carotenoids. The greater proportion of Lutein in the pediatric brain suggests a need for Lutein during neural development as well. In adults, higher Lutein status is related to better cognitive performance, and Lutein supplementation improves cognition. The evidence to date warrants further investigation into the role of Lutein and zeaxanthin in visual and cognitive health throughout the lifespan.
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A possible role for Lutein and zeaxanthin in cognitive function in the elderly
The American journal of clinical nutrition, 2012Co-Authors: Elizabeth J JohnsonAbstract:Epidemiologic studies suggest that dietary Lutein and zeaxanthin may be of benefit in maintaining cognitive health. Among the carotenoids, Lutein and zeaxanthin are the only two that cross the blood-retina barrier to form macular pigment (MP) in the eye. They also preferentially accumulate in the human brain. Lutein and zeaxanthin in macula from nonhuman primates were found to be significantly correlated with their concentrations in matched brain tissue. Therefore, MP can be used as a biomarker of Lutein and zeaxanthin in primate brain tissue. This is of interest given that a significant correlation was found between MP density and global cognitive function in healthy older adults. An examination of a relation between cognition and Lutein and zeaxanthin concentrations in the brain tissue of decedents from a population-based study in centenarians found that zeaxanthin concentrations in brain tissue were significantly related to antemortem measures of global cognitive function, memory retention, verbal fluency, and dementia severity after adjustment for age, sex, education, hypertension, and diabetes. In univariate analyses, Lutein was related to recall and verbal fluency, but the strength of the associations was attenuated with adjustment for covariates. However, Lutein concentrations in the brain were significantly lower in individuals with mild cognitive impairment than in those with normal cognitive function. Last, in a 4-mo, double-blinded, placebo-controlled trial in older women that involved Lutein supplementation (12 mg/d), alone or in combination with DHA (800 mg/d), verbal fluency scores improved significantly in the DHA, Lutein, and combined-treatment groups. Memory scores and rate of learning improved significantly in the combined-treatment group, who also showed a trend toward more efficient learning. When all of these observations are taken into consideration, the idea that Lutein and zeaxanthin can influence cognitive function in older adults warrants further study.
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Lutein and zeaxanthin supplementation reduces photooxidative damage and modulates the expression of inflammation related genes in retinal pigment epithelial cells
Free Radical Biology and Medicine, 2012Co-Authors: Qingning Bian, Elizabeth J Johnson, Guangwen Tang, Shasha Gao, Jilin Zhou, Jian Qin, Allen Taylor, Janet R Sparrow, Dennis GierhartAbstract:Oxidative damage and inflammation are related to the pathogenesis of age-related macular degeneration (AMD). Epidemiologic studies suggest that insufficient dietary Lutein and zeaxanthin intake or lower serum zeaxanthin levels are associated with increased risk for AMD. The objective of this work is to test the protective effects of Lutein and zeaxanthin against photooxidative damage to retinal pigment epithelial cells (RPE) and oxidation-induced changes in expression of inflammation-related genes. To mimic lipofuscin-mediated photooxidation in vivo, we used ARPE-19 cells that accumulated A2E, a lipofuscin fluorophore and photosensitizer, as a model system to investigate the effects of Lutein and zeaxanthin supplementation. The data show that supplementation with Lutein or zeaxanthin in the medium resulted in accumulation of Lutein or zeaxanthin in the RPE cells. The concentrations of Lutein and zeaxanthin in the cells were 2- to 14-fold of that detected in the medium, indicating that ARPE-19 cells actively take up Lutein or zeaxanthin. As compared with untreated cells, exposure of A2E-containing RPE to blue light resulted in a 40-60% decrease in proteasome activity, a 50-80% decrease in expression of CFH and MCP-1, and an~20-fold increase in expression of IL-8. The photooxidation-induced changes in expression of MCP-1, IL-8, and CFH were similar to those caused by chemical inhibition of the proteasome, suggesting that inactivation of the proteasome is involved in the photooxidation-induced alteration in expression of these inflammation-related genes. Incubation of the A2E-containing RPE with Lutein or zeaxanthin prior to blue light exposure significantly attenuated the photooxidation-induced inactivation of the proteasome and photooxidation-induced changes in expression of MCP-1, IL-8, and CFH. Together, these data indicate that Lutein or zeaxanthin modulates inflammatory responses in cultured RPE in response to photooxidation. Protecting the proteasome from oxidative inactivation appears to be one of the mechanisms by which Lutein and zeaxanthin modulate the inflammatory response. Similar mechanisms may explain salutary effects of Lutein and zeaxanthin in reducing the risk for AMD.
Ming Chang Chan - One of the best experts on this subject based on the ideXlab platform.
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effects of nitrogen source availability and bioreactor operating strategies on Lutein production with scenedesmus obliquus fsp 3
Bioresource Technology, 2015Co-Authors: Youping Xie, Duu-jong Lee, Ming Chang Chan, Chun Yen Chen, Chiehchen Huang, Chen Chun Liu, Jo Shu ChangAbstract:In this study, the effects of the type and concentration of nitrogen sources on the cell growth and Lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest Lutein content (4.61 mg/g) and Lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the Lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and Lutein productivity (6.01 mg/L/day). This performance is better than most related studies.
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enhancing Lutein productivity of an indigenous microalga scenedesmus obliquus fsp 3 using light related strategies
Bioresource Technology, 2014Co-Authors: Ming Chang Chan, Duu-jong Lee, Chun Yen Chen, Chen Chun Liu, Wen Lung Lee, Jo Shu ChangAbstract:Lutein, one of the main photosynthetic pigments, is a promising natural product with both nutritional and pharmaceutical applications. In this study, light-related strategies were applied to enhance the cell growth and Lutein production of a Lutein-rich microalga Scenedesmus obliquus FSP-3. The results demonstrate that using white LED resulted in better Lutein production efficiency when compared to the other three monochromatic LEDs (red, blue, and green). The Lutein productivity of S. obliquus FSP-3 was further improved by adjusting the type of light source and light intensity. The optimal Lutein productivity of 4.08 mg/L/d was obtained when using a TL5 fluorescent lamp at a light intensity of 300 μmol/m(2)/s, and this performance is better than that reported in most related studies. Moreover, the time-course profile of Lutein accumulation in the microalga shows that the maximal Lutein content and productivity were obtained at the onset of nitrogen depletion.
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characterization extraction and purification of Lutein produced by an indigenous microalga scenedesmus obliquus cnw n
Biochemical Engineering Journal, 2013Co-Authors: Ming Chang Chan, Duu-jong Lee, Chun Yen Chen, Chiehchen Huang, Jo Shu ChangAbstract:Abstract This study aimed to improve the commercial viability of microalgae-based Lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high Lutein content of over 0.25%. Effective Lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24 h. Diethyl ether exhibited the best Lutein extraction efficiency. Storage of the Lutein extract at low temperature (4 or −20 °C) with antioxidant addition (around 0.01% BHT) can maintain 90% Lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of Lutein extracts under the exposure of light. The protocol developed in this work allows efficient Lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of Lutein and its commercial value.
Chun Yen Chen - One of the best experts on this subject based on the ideXlab platform.
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a highly efficient two stage cultivation strategy for Lutein production using heterotrophic culture of chlorella sorokiniana mb 1 m12
Bioresource Technology, 2018Co-Authors: Chun Yen Chen, Duu-jong Lee, Dillirani Nagarajan, Chien Hsiang Chang, Jo Shu ChangAbstract:Abstract A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce Lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal Lutein content (3.67 mg Lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the Lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, Lutein content and Lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high Lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic Lutein production in commercial scale.
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effects of nitrogen source availability and bioreactor operating strategies on Lutein production with scenedesmus obliquus fsp 3
Bioresource Technology, 2015Co-Authors: Youping Xie, Duu-jong Lee, Ming Chang Chan, Chun Yen Chen, Chiehchen Huang, Chen Chun Liu, Jo Shu ChangAbstract:In this study, the effects of the type and concentration of nitrogen sources on the cell growth and Lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest Lutein content (4.61 mg/g) and Lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the Lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and Lutein productivity (6.01 mg/L/day). This performance is better than most related studies.
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enhancing Lutein productivity of an indigenous microalga scenedesmus obliquus fsp 3 using light related strategies
Bioresource Technology, 2014Co-Authors: Ming Chang Chan, Duu-jong Lee, Chun Yen Chen, Chen Chun Liu, Wen Lung Lee, Jo Shu ChangAbstract:Lutein, one of the main photosynthetic pigments, is a promising natural product with both nutritional and pharmaceutical applications. In this study, light-related strategies were applied to enhance the cell growth and Lutein production of a Lutein-rich microalga Scenedesmus obliquus FSP-3. The results demonstrate that using white LED resulted in better Lutein production efficiency when compared to the other three monochromatic LEDs (red, blue, and green). The Lutein productivity of S. obliquus FSP-3 was further improved by adjusting the type of light source and light intensity. The optimal Lutein productivity of 4.08 mg/L/d was obtained when using a TL5 fluorescent lamp at a light intensity of 300 μmol/m(2)/s, and this performance is better than that reported in most related studies. Moreover, the time-course profile of Lutein accumulation in the microalga shows that the maximal Lutein content and productivity were obtained at the onset of nitrogen depletion.
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characterization extraction and purification of Lutein produced by an indigenous microalga scenedesmus obliquus cnw n
Biochemical Engineering Journal, 2013Co-Authors: Ming Chang Chan, Duu-jong Lee, Chun Yen Chen, Chiehchen Huang, Jo Shu ChangAbstract:Abstract This study aimed to improve the commercial viability of microalgae-based Lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high Lutein content of over 0.25%. Effective Lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24 h. Diethyl ether exhibited the best Lutein extraction efficiency. Storage of the Lutein extract at low temperature (4 or −20 °C) with antioxidant addition (around 0.01% BHT) can maintain 90% Lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of Lutein extracts under the exposure of light. The protocol developed in this work allows efficient Lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of Lutein and its commercial value.
