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Zainudin Bachok - One of the best experts on this subject based on the ideXlab platform.
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Variation in fatty acid composition of the bigeye snapper Lutjanus Lutjanus collected in coral reef habitats of the Malaysian South China Sea.
Journal of biological research (Thessalonike Greece), 2015Co-Authors: Takaomi Arai, Razikin Amalina, Zainudin BachokAbstract:Background In order to understand trophic ecology, habitat use and migration of coral reef fish, fatty acid composition and levels were examined in the bigeye snapper Lutjanus Lutjanus collected in the Malaysian South China Sea.
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Variation in fatty acid composition of the bigeye snapper Lutjanus Lutjanus collected in coral reef habitats of the Malaysian South China Sea
Journal of Biological Research-Thessaloniki, 2015Co-Authors: Takaomi Arai, Razikin Amalina, Zainudin BachokAbstract:Background In order to understand trophic ecology, habitat use and migration of coral reef fish, fatty acid composition and levels were examined in the bigeye snapper Lutjanus Lutjanus collected in the Malaysian South China Sea. Results Proportions of saturated fatty acids (SAFA) ranged from 55.0% to 66.5%, with the highest proportions in fatty acids, the second highest was monounsaturated fatty acids (MUFA) ranged from 30.7% to 40.2% while the proportion of polyunsaturated fatty acids (PUFA) was the lowest ranged from 2.8% to 4.8%. Palmitic acid (16:0) was the most common in SAFA, oleic acid (C18:1ω9c) was the dominant in MUFA and linolenic acid (C18:3n3) showed the highest value in PUFA. Fatty acid concentrations, especially in SAFA and MUFA, increased with fish growth, suggesting diet and habitat shifts during the fish life history. Most of the fish had more than 1 of EPA: DHA ratio, which suggested that diets of L. Lutjanus tended to be higher trophic organisms such as zooplankton and crustacean in coral ecosystem. Conclusions The diet shift revealed by the composition and levels of the fatty acid profile revealed potential pattern in the habitat use and migration scale in coral reef environment of L. Lutjanus .
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Fatty acid composition indicating diverse habitat use in coral reef fishes in the Malaysian South China Sea.
Biological Research, 2015Co-Authors: Takaomi Arai, Razikin Amalina, Zainudin BachokAbstract:Background In order to understand feeding ecology and habitat use of coral reef fish, fatty acid composition was examined in five coral reef fishes, Thalassoma lunare, Lutjanus Lutjanus, Abudefduf bengalensis, Scarus rivulatus and Scolopsis affinis collected in the Bidong Island of Malaysian South China Sea.
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Fatty acid composition indicating diverse habitat use in coral reef fishes in the Malaysian South China Sea
Biological Research, 2015Co-Authors: Takaomi Arai, Razikin Amalina, Zainudin BachokAbstract:Background In order to understand feeding ecology and habitat use of coral reef fish, fatty acid composition was examined in five coral reef fishes, Thalassoma lunare , Lutjanus Lutjanus , Abudefduf bengalensis , Scarus rivulatus and Scolopsis affinis collected in the Bidong Island of Malaysian South China Sea. Results Proportions of saturated fatty acids (SAFA) ranged 57.2% 74.2%, with the highest proportions in fatty acids, the second highest was monounsaturated fatty acids (MUFA) ranged from 21.4% to 39.0% and the proportion of polyunsaturated fatty acids (PUFA) was the lowest ranged from 2.8% to 14.1%. Each fatty acid composition differed among fishes, suggesting diverse feeding ecology, habitat use and migration during the fishes’ life history in the coral reef habitats. Conclusions Diets of the coral fish species might vary among species in spite of that each species are living sympatrically. Differences in fatty acid profiles might not just be considered with respect to the diets, but might be based on the habitat and migration.
Brian W. Bowen - One of the best experts on this subject based on the ideXlab platform.
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biogeographical barriers: perspectives from two widespread Indo-Pacific snappers (Lutjanus kasmira and Lutjanus fulvus)
2010Co-Authors: Michelle R. Gaither, Robert J. Toonen, D. Ross Robertson, Serge Planes, Brian W. BowenAbstract:Aim In the Indo-Pacific, the mass of islands of the Indonesian archipelago constitute a major biogeographical barrier (the Indo-Pacific Barrier, IPB) separating the Pacific and Indian oceans. Evidence for other, more localized barriers include high rates of endemism at the Marquesas and other isolated peripheral islands in the Pacific. Here we use mitochondrial-sequence comparisons to evaluate the efficacy of biogeographical barriers on populations of the snappers Lutjanus kasmira and Lutjanus fulvus across their natural ranges. Location Pacific and Indian oceans. Methods Mitochondrial cytochrome b sequence data were obtained from 370 individuals of L. kasmira and 203 individuals of L. fulvus collected from across each species’ range. Allele frequency data for two nuclear introns were collected from L. kasmira. Phylogenetic and population-level analyses were used to determine patterns of population structure in these species and to identify barriers to dispersal. Results Lutjanus kasmira lacks genetic structure across the IPB and throughout 12,000 km of its central Indo-Pacific range. In contrast, L. fulvus demonstrates high levels of population structure at all geographical scales. In both species, highly significant population structure results primarily from the phylogenetic distinctiveness of their Marquesas Islands populations (L. kasmira, d = 0.50‐ 0.53%; L. fulvus, d = 0.87‐1.50%). Coalescence analyses of the L. kasmira data indicate that populations at opposite ends of its range (western Indian Ocean and the Marquesas) are the oldest. Coalescence analyses for L. fulvus are less robust but also indicate colonization from the Indian to the Pacific Ocean. Main conclusions The IPB does not act as a biogeographical barrier to L. kasmira, and, in L. fulvus, its effects are no stronger than isolating mechanisms elsewhere. Both species demonstrate a strong genetic break at the Marquesas. Population divergence and high endemism in that archipelago may be a product of geographical isolation enhanced by oceanographic currents that limit gene flow to and from those islands, and adaptation to unusual ecological conditions. Lutjanus kasmira shows evidence of Pleistocene population expansion throughout the Indo-central Pacific that originated in the western Indian Ocean rather than the Marquesas, further demonstrating a strong barrier at the latter location.
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Genetic evaluation of marine biogeographical barriers: perspectives from two widespread Indo-Pacific snappers (Lutjanus kasmira and Lutjanus fulvus)
Journal of Biogeography, 2009Co-Authors: Michelle R. Gaither, Robert J. Toonen, D. Ross Robertson, Serge Planes, Brian W. BowenAbstract:Aim In the Indo-Pacific, the mass of islands of the Indonesian archipelago constitute a major biogeographical barrier (the Indo-Pacific Barrier, IPB) separating the Pacific and Indian oceans. Evidence for other, more localized barriers include high rates of endemism at the Marquesas and other isolated peripheral islands in the Pacific. Here we use mitochondrial-sequence comparisons to evaluate the efficacy of biogeographical barriers on populations of the snappers Lutjanus kasmira and Lutjanus fulvus across their natural ranges. Location Pacific and Indian oceans. Methods Mitochondrial cytochrome b sequence data were obtained from 370 individuals of L. kasmira and 203 individuals of L. fulvus collected from across each species' range. Allele frequency data for two nuclear introns were collected from L. kasmira. Phylogenetic and population-level analyses were used to determine patterns of population structure in these species and to identify barriers to dispersal. Results Lutjanus kasmira lacks genetic structure across the IPB and throughout 12,000 km of its central Indo-Pacific range. In contrast, L. fulvus demonstrates high levels of population structure at all geographical scales. In both species, highly significant population structure results primarily from the phylogenetic distinctiveness of their Marquesas Islands populations (L. kasmira, d = 0.50-0.53%; L. fulvus, d = 0.87-1.50%). Coalescence analyses of the L. kasmira data indicate that populations at opposite ends of its range (western Indian Ocean and the Marquesas) are the oldest. Coalescence analyses for L. fulvus are less robust but also indicate colonization from the Indian to the Pacific Ocean. Main conclusions The IPB does not act as a biogeographical barrier to L. kasmira, and, in L. fulvus, its effects are no stronger than isolating mechanisms elsewhere. Both species demonstrate a strong genetic break at the Marquesas. Population divergence and high endemism in that archipelago may be a product of geographical isolation enhanced by oceanographic currents that limit gene flow to and from those islands, and adaptation to unusual ecological conditions. Lutjanus kasmira shows evidence of Pleistocene population expansion throughout the Indo-central Pacific that originated in the western Indian Ocean rather than the Marquesas, further demonstrating a strong barrier at the latter location.
Liangmin Huang - One of the best experts on this subject based on the ideXlab platform.
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species identification in salted products of red snappers by semi nested pcr rflp based on the mitochondrial 12s rrna gene sequence
Food Control, 2006Co-Authors: Junbin Zhang, Hui Huang, Zeping Cai, Liangmin HuangAbstract:A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.
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Population genetic structure of crimson snapper Lutjanus erythropterus in East Asia, revealed by analysis of the mitochondrial control region
ICES Journal of Marine Science, 2006Co-Authors: Junbin Zhang, Zeping Cai, Liangmin HuangAbstract:The population genetic structure of the crimson snapper Lutjanus erythropterus in East Asia was examined with a 427-bp hypervariable portion of the mtDNA control region. A total of 262 samples were collected and 75 haplotypes were obtained. Neutrality tests (Tajima's and Fu's) suggested that Lutjanus erythropterus in East Asia had experienced a bottleneck followed by population expansion since the late Pleistocene. Despite the low phylogeographic structures in mtDNA haplotypes, a hierarchical examination of populations in 11 localities from four geographical regions using analysis of molecular variance (AMOVA) indicated significant genetic differentiation among regions (Phi(CT) = 0.08564, p < 0.01). Limited gene flow between the eastern region (including a locality in the western Pacific Ocean and two localities in the East Sea) and three geographic regions of the South China Sea largely contributed to the genetic subdivision. However, comparisons among three geographic regions of the South China Sea showed little to no genetic difference. Populations of Lutjanus erythropterus in East Asia are inferred to be divided into two major groups: an eastern group, including populations of the western Pacific Ocean and the East Sea, and a South China Sea group, consisting of populations from northern Malaysia to South China. The results suggest that fishery management should reflect the genetic differentiation and diversity in East Asia. (c) 2006 International Council for the Exploration of the Sea. Published by Elsevier Ltd. All rights reserved.
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Larval identification of Lutjanus Bloch in Nansha coral reefs by AFLP molecular method
Journal of Experimental Marine Biology and Ecology, 2004Co-Authors: Junbin Zhang, Liangmin Huang, Heqiang HuoAbstract:Abstract Species of Lutjanus Bloch are highly valued fish in some fisheries of the world and some species are cultured in the Southeast Asia especially in South China. Wild larvae are still the major source of mariculture in South China because artificial breeding techniques for most Lutjanus species have not yet been available. The Nansha coral reefs (also called Spratly Archipelago) water area, which is located in the South China Sea, is the main habitat and spawn area for Lutjanus in China. Larval identification of Lutjanus is important for relative ecological studies and mariculture, but larvae of many closely related species, such as those of the genus Lutjanus , are different to be distinguished morphologically. In the present study, a PCR-based fingerprinting technique called amplified fragment length polymorphism AFLP was used in the characterization and identification of 11 Lutjanus species captured in Nansha coral reefs. Optimal AFLP patterns were obtained with primer combination of E+AGC/M+CAA selective nucleotides. There were in total 132 AFLP loci in all specimens, and AFLP markers of each species varied from 44 to 69, but only 7 markers were fixed in all specimens. Meanwhile, high levels of intraspecific homogeneity were observed. All 11 species of Lutjanus were successfully identified by the comparative analyses of AFLP patterns. Moreover, neighbour-joining and UPGMA analyses of AFLP data were compared with current morphological taxonomic systems.
Darlina Naim - One of the best experts on this subject based on the ideXlab platform.
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DNA barcoding of Malaysian commercial snapper reveals an unrecognized species of the yellow-lined Lutjanus (Pisces:Lutjanidae)
PLOS ONE, 2018Co-Authors: Adibah Abu Bakar, Eleanor A. S. Adamson, Lia Halim Juliana, Siti Azizah Nor Mohd, Darlina NaimAbstract:Management of wild fisheries resources requires accurate knowledge on which species are being routinely exploited, but it can be hard to identify fishes to species level, especially in speciose fish groups where colour patterns vary with age. Snappers of the genus Lutjanus represent one such group, where fishes can be hard to identify and as a result fisheries statistics fail to capture species-level taxonomic information. This study employs traditional morphological and DNA barcoding approaches to identify adult and juvenile Lutjanus species harvested in Malaysian waters. Our results reveal a suite of species that differs markedly from those that have previously been considered important in the Malaysian wild-capture fishery and show that official fisheries statistics do not relate to exploitation at the species level. Furthermore, DNA barcoding uncovered two divergent groups of bigeye snapper ('Lutjanus Lutjanus') distributed on either side of the Malay Peninsula, displaying a biogeographical pattern similar to distributions observed for many co-occurring reef-distributed fish groups. One of these bigeye snapper groups almost certainly represents an unrecognized species in need of taxonomic description. The study demonstrates the utility of DNA barcoding in uncovering overlooked diversity and for assessing species catch composition in a complicated but economically important taxonomic group.
Junbin Zhang - One of the best experts on this subject based on the ideXlab platform.
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species identification in salted products of red snappers by semi nested pcr rflp based on the mitochondrial 12s rrna gene sequence
Food Control, 2006Co-Authors: Junbin Zhang, Hui Huang, Zeping Cai, Liangmin HuangAbstract:A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.
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Population genetic structure of crimson snapper Lutjanus erythropterus in East Asia, revealed by analysis of the mitochondrial control region
ICES Journal of Marine Science, 2006Co-Authors: Junbin Zhang, Zeping Cai, Liangmin HuangAbstract:The population genetic structure of the crimson snapper Lutjanus erythropterus in East Asia was examined with a 427-bp hypervariable portion of the mtDNA control region. A total of 262 samples were collected and 75 haplotypes were obtained. Neutrality tests (Tajima's and Fu's) suggested that Lutjanus erythropterus in East Asia had experienced a bottleneck followed by population expansion since the late Pleistocene. Despite the low phylogeographic structures in mtDNA haplotypes, a hierarchical examination of populations in 11 localities from four geographical regions using analysis of molecular variance (AMOVA) indicated significant genetic differentiation among regions (Phi(CT) = 0.08564, p < 0.01). Limited gene flow between the eastern region (including a locality in the western Pacific Ocean and two localities in the East Sea) and three geographic regions of the South China Sea largely contributed to the genetic subdivision. However, comparisons among three geographic regions of the South China Sea showed little to no genetic difference. Populations of Lutjanus erythropterus in East Asia are inferred to be divided into two major groups: an eastern group, including populations of the western Pacific Ocean and the East Sea, and a South China Sea group, consisting of populations from northern Malaysia to South China. The results suggest that fishery management should reflect the genetic differentiation and diversity in East Asia. (c) 2006 International Council for the Exploration of the Sea. Published by Elsevier Ltd. All rights reserved.
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Larval identification of Lutjanus Bloch in Nansha coral reefs by AFLP molecular method
Journal of Experimental Marine Biology and Ecology, 2004Co-Authors: Junbin Zhang, Liangmin Huang, Heqiang HuoAbstract:Abstract Species of Lutjanus Bloch are highly valued fish in some fisheries of the world and some species are cultured in the Southeast Asia especially in South China. Wild larvae are still the major source of mariculture in South China because artificial breeding techniques for most Lutjanus species have not yet been available. The Nansha coral reefs (also called Spratly Archipelago) water area, which is located in the South China Sea, is the main habitat and spawn area for Lutjanus in China. Larval identification of Lutjanus is important for relative ecological studies and mariculture, but larvae of many closely related species, such as those of the genus Lutjanus , are different to be distinguished morphologically. In the present study, a PCR-based fingerprinting technique called amplified fragment length polymorphism AFLP was used in the characterization and identification of 11 Lutjanus species captured in Nansha coral reefs. Optimal AFLP patterns were obtained with primer combination of E+AGC/M+CAA selective nucleotides. There were in total 132 AFLP loci in all specimens, and AFLP markers of each species varied from 44 to 69, but only 7 markers were fixed in all specimens. Meanwhile, high levels of intraspecific homogeneity were observed. All 11 species of Lutjanus were successfully identified by the comparative analyses of AFLP patterns. Moreover, neighbour-joining and UPGMA analyses of AFLP data were compared with current morphological taxonomic systems.
