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James M Slavicek - One of the best experts on this subject based on the ideXlab platform.
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A comparison of the adaptations of strains of Lymantria dispar multiple nucleopolyhedrovirus to hosts from spatially isolated populations
Journal of invertebrate pathology, 2017Co-Authors: V V Martemyanov, Olga A Baturina, James M Slavicek, Marsel R. Kabilov, Sergey V. Pavlushin, John D. Podgwaite, I.a. Belousova, Alexandr V. IlyinykhAbstract:The adaptation of pathogens to either their hosts or to environmental conditions is the focus of many current ecological studies. In this work we compared the ability of six spatially-distant Lymantria dispar (gypsy moth) multiple nucleopolyhedrovirus (LdMNPV) strains (three from eastern North America and three from central Asia) to induce acute infection in gypsy moth larvae. We also sequenced the complete genome of one Asian (LdMNPV-27/0) and one North American (LdMNPV-45/0) strain which were used for bioassay. We found that all of the North American virus strains, with the exception of one, demonstrated higher potency than the Asian virus strains, either in North American (Lymantria dispar) larvae or, in Asian (Lymantria dispar asiatica) larvae. Complete genome sequencing revealed two gene deletions in the LdMNPV-27/0 strain: the virus enhancin factor gene (vef-1) and the baculovirus repeated orf gene (bro-p). These deletions were not seen in the LdMNPV-45/0 strain nor in other American strains available in archiving systems. We also found deletions of the bro-e and bro-o genes in LdMNPV-45/0 strain but not in the LdMNPV-27/0 strain. The phylogenetic inference with an alignment of the 37 core gene nucleotide sequences revealed the close relationship of the LdMNPV-45/0 strain with other American strains accessed in GenBank (Ab-a624 and 5-6) while the LdMNPV-27/0 strain was clustered together with the LdMNPV-3054 strain (isolated in Spain) instead of predicted clustering with LdMNPV- 3029 (isolated in Asia). Our study demonstrated that first, different LdMNPV isolates from the same metapopulations of L. dispar exhibit little or no difference in the degree of virulence towards host larvae and second, that locality of host population is not an important driver of LdMNPV virulence. Virulence of LdMNPV is determined only by viral genetics. The genetic differences between North American and Central Asian virus strains are discussed.
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pathogenesis of Lymantria dispar multiple nucleopolyhedrovirus in l dispar and mechanisms of developmental resistance
Journal of General Virology, 2010Co-Authors: Jim Mcneil, Diana Coxfoster, James M Slavicek, Matthew Gardner, Suzanne M. Thiem, Kelli HooverAbstract:Lymantria dispar has a long historical association with the baculovirus Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV), which is one of the primary population regulators of L. dispar in the field. However, host larvae exhibit strong developmental resistance to fatal infection by LdMNPV; the LD50 in newly moulted fourth instars is 18-fold lower than in the middle of the instar (48–72 h post-moult). Using a recombinant of LdMNPV expressing lacZ, we examined the key steps of pathogenesis in the host to explore mechanisms of developmental resistance. At the midgut level, we observed reduced primary midgut infections in mid-fourth instars, indicating increased sloughing of infected cells. Additional barriers were observed as the virus escaped the midgut. Mid-fourth instars had higher numbers of melanized foci of infection associated with the midgut, apoptotic tracheal epidermal cells and haemocytes, and reduced numbers of infected haemocytes later in infection. Our results show that the co-evolutionary relationship between L. dispar and LdMNPV has resulted in both midgut-based and systemic antiviral defences and that these defences are age-dependent within the instar. This age-related susceptibility may contribute to how the virus is maintained in nature and could influence management of L. dispar by using the virus.
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n glycan structures of human transferrin produced by Lymantria dispar gypsy moth cells using the ldmnpv expression system
Glycobiology, 2003Co-Authors: One Choi, Noboru Tomiya, James M Slavicek, Michael J. BetenbaughAbstract:N-glycan structures of recombinant human serum transferrin (hTf) expressed by Lymantria dispar (gypsy moth) 652Y cells were determined. The gene encoding hTf was incorporated into a Lymantria dispar nucleopolyhedrovirus (LdMNPV) under the control of the polyhedrin promoter. This virus was then used to infect Ld652Y cells, and the recombinant protein was harvested at 120 h postinfection. N-glycans were released from the purified recombinant human serum transferrin and derivatized with 2-aminopyridine; the glycan structures were analyzed by a two-dimensional HPLC and MALDI-TOF MS. Structures of 11 glycans (88.8% of total N-glycans) were elucidated. The glycan analysis revealed that the most abundant glycans were Man1‐3(Fuca6)GlcNAc2 (75.5%) and GlcNAcMan3(Fuca6)GlcNAc2 (7.4%). There was only ~6% of high-mannose type glycans identified. Nearly half (49.8%) of the total N-glycans contained a(1,6)-fucosylation on the Asn-linked GlcNAc residue. However a(1,3)fucosylation on the same GlcNAc, often found in N-glycans produced by other insects and insect cells, was not detected. Inclusion of fetal bovine serum in culture media had little effect on the N-glycan structures of the recombinant human serum transferrin obtained.
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Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus
Virus Research, 1997Co-Authors: Christopher I. Riegel, James M SlavicekAbstract:Abstract The life cycle of the Lymantria dispar nuclear polyhedrosis virus (Ld M NPV) was characterized through analysis of budded virus (BV) release, the temporal formation of polyhedra, the temporal transcription pattern of representative early, late, and hyper-expressed late genes, and the onset of DNA replication in the Ld652Y cell line. Transcripts from the Ld M NPV immediate early gene G22 were detected 4 h post infection (h p.i.). The late and hyper-expressed late p39 capsid and polyhedrin genes were initially transcribed at approximately 20 and 24 h p.i., respectively. Viral DNA replication initiated at approximately 18–20 h p.i. Budded virus was released from infected cells between 24 and 36 h p.i., and polyhedra were first detected at approximately 48 h p.i.
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CHARACTERIZATION OF THE Lymantria dispar NUCLEOPOLYHEDROVIRUS 25K FP GENE
Journal of General Virology, 1996Co-Authors: D S Bischoff, James M SlavicekAbstract:The Lymantria dispar nucleopolyhedrovirus (LdMNPV) gene encoding the 25K FP protein has been cloned and sequenced. The 25K FP gene codes for a 217 amino acid protein with a predicted molecular mass of 24870 Da. Expression of the 25K FP protein in a rabbit reticulocyte system generated a 27 kDa protein, in close agreement with the molecular mass predicted from the nucleotide sequence. The gene is located between 40.3 and 40.8 map units on the viral genome. It is respect to the circular map at late times during the infection cycle from a consensus baculovirus late promoter. The LdMNPV and Autographa californica nucleopolyhedrovirus (AcMNPV) 25K FP proteins exhibit 52% amino acid identity with several regions showing greater than 75% identity. Homologues to the AcMNPV orf59 and orf60 were also identified upstream (with respect to the genome) of the 25K FP gene in LdMNPV and exhibit 52% and 45% amino acid identity, respectively.
V V Martemyanov - One of the best experts on this subject based on the ideXlab platform.
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A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in Lymantria dispar L.
Insects, 2020Co-Authors: Yuriy B. Akhanaev, Irina A. Belousova, Darya A. Lebedeva, Sergey V. Pavlushin, V V MartemyanovAbstract:Baculoviruses can persist in insect host organisms after infection and may be vertically transmitted to the next generation, in which they may be reactivated. The goal of the present study was to compare the efficiency of the vertical transmission of high- and low-virulence strains and the subsequent reactivation of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) in the offspring of Lymantria dispar L. adults who survived after viral infection. As a result of parental infection, the fecundity of survived females, pupae weight, and fertility were significantly different compared to the untreated insects. However, differences in these parameters between high- and low-virulence strains were not observed. The prevalence of virus strains in the offspring measured by quantitative polymerase chain reaction also did not differ. When the larvae reached the fourth instar, they were starved to activate the vertically transmitted virus. The frequency of virus activation in the experiment was not dependent on the virulence of the virus strains. These results are helpful for understanding the strategy of virus survival in nature and for the selection of the most effective strains with transgenerational effects in the years following pest treatment.
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A comparison of the adaptations of strains of Lymantria dispar multiple nucleopolyhedrovirus to hosts from spatially isolated populations
Journal of invertebrate pathology, 2017Co-Authors: V V Martemyanov, Olga A Baturina, James M Slavicek, Marsel R. Kabilov, Sergey V. Pavlushin, John D. Podgwaite, I.a. Belousova, Alexandr V. IlyinykhAbstract:The adaptation of pathogens to either their hosts or to environmental conditions is the focus of many current ecological studies. In this work we compared the ability of six spatially-distant Lymantria dispar (gypsy moth) multiple nucleopolyhedrovirus (LdMNPV) strains (three from eastern North America and three from central Asia) to induce acute infection in gypsy moth larvae. We also sequenced the complete genome of one Asian (LdMNPV-27/0) and one North American (LdMNPV-45/0) strain which were used for bioassay. We found that all of the North American virus strains, with the exception of one, demonstrated higher potency than the Asian virus strains, either in North American (Lymantria dispar) larvae or, in Asian (Lymantria dispar asiatica) larvae. Complete genome sequencing revealed two gene deletions in the LdMNPV-27/0 strain: the virus enhancin factor gene (vef-1) and the baculovirus repeated orf gene (bro-p). These deletions were not seen in the LdMNPV-45/0 strain nor in other American strains available in archiving systems. We also found deletions of the bro-e and bro-o genes in LdMNPV-45/0 strain but not in the LdMNPV-27/0 strain. The phylogenetic inference with an alignment of the 37 core gene nucleotide sequences revealed the close relationship of the LdMNPV-45/0 strain with other American strains accessed in GenBank (Ab-a624 and 5-6) while the LdMNPV-27/0 strain was clustered together with the LdMNPV-3054 strain (isolated in Spain) instead of predicted clustering with LdMNPV- 3029 (isolated in Asia). Our study demonstrated that first, different LdMNPV isolates from the same metapopulations of L. dispar exhibit little or no difference in the degree of virulence towards host larvae and second, that locality of host population is not an important driver of LdMNPV virulence. Virulence of LdMNPV is determined only by viral genetics. The genetic differences between North American and Central Asian virus strains are discussed.
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Complete Genome Sequence of a Western Siberian Lymantria dispar Multiple Nucleopolyhedrovirus Isolate
Genome Announcements, 2015Co-Authors: Marsel R. Kabilov, Alexander Bondar, Olga A Baturina, V V Martemyanov, Aleksey E. Tupikin, Irina A. Belousova, Alexandr V. IlyinykhAbstract:ABSTRACT A novel strain of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV-27) was isolated from dead larvae of a Western Siberian (WS) population of gypsy moths (Lymantria dispar L.). We report the complete genome sequence of this strain, comprising 164,108 bp and double-stranded circular DNA encoding 162 predicted open reading frames.
George F. Rohrmann - One of the best experts on this subject based on the ideXlab platform.
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Nucleotide sequence of the polyhedron envelope protein gene region of the Lymantria dispar nuclear polyhedrosis virus.
Journal of General Virology, 1992Co-Authors: Rebecca M. Bjornson, George F. RohrmannAbstract:A 6.4 kb region from the Lymantria dispar multicapsid nuclear polyhedrosis virus (LdMNPV) genome was sequenced and found to contain open reading frames (ORFs) homologous to the polyhedron envelope (PE) protein coding sequence, and the C-terminal half of ORF 1, which is a gene located upstream of the PE protein gene in other baculoviruses. The proteins predicted from the LdMNPV genes encoding the PE protein, and ORF 1 demonstrated 27 and 34% amino acid sequence identity, respectively, with the corresponding genes in the Autographa californica multicapsid nuclear polyhedrosis virus.
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Nucleotide sequence of the p39-capsid gene region of the Lymantria dispar nuclear polyhedrosis virus.
Journal of General Virology, 1992Co-Authors: Rebecca M. Bjornson, George F. RohrmannAbstract:A 1.85 kb region, containing an open reading frame (ORF) homologous to the baculovirus p39-capsid gene, was sequenced from the Lymantria dispar multicapsid nuclear polyhedrosis virus (LdMNPV) genome. Analysis of the p39-capsid gene demonstrated that it was 39% and 47% identical in amino acid sequence with the homologous genes in the Autographa californica and Orgyia pseudotsugata MNPVs, respectively. Two late promoter elements located upstream of the p39 gene in the LdMNPV genome are conserved with two other baculoviruses, whereas an ORF located downstream is not conserved.
Alexandr V. Ilyinykh - One of the best experts on this subject based on the ideXlab platform.
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A comparison of the adaptations of strains of Lymantria dispar multiple nucleopolyhedrovirus to hosts from spatially isolated populations
Journal of invertebrate pathology, 2017Co-Authors: V V Martemyanov, Olga A Baturina, James M Slavicek, Marsel R. Kabilov, Sergey V. Pavlushin, John D. Podgwaite, I.a. Belousova, Alexandr V. IlyinykhAbstract:The adaptation of pathogens to either their hosts or to environmental conditions is the focus of many current ecological studies. In this work we compared the ability of six spatially-distant Lymantria dispar (gypsy moth) multiple nucleopolyhedrovirus (LdMNPV) strains (three from eastern North America and three from central Asia) to induce acute infection in gypsy moth larvae. We also sequenced the complete genome of one Asian (LdMNPV-27/0) and one North American (LdMNPV-45/0) strain which were used for bioassay. We found that all of the North American virus strains, with the exception of one, demonstrated higher potency than the Asian virus strains, either in North American (Lymantria dispar) larvae or, in Asian (Lymantria dispar asiatica) larvae. Complete genome sequencing revealed two gene deletions in the LdMNPV-27/0 strain: the virus enhancin factor gene (vef-1) and the baculovirus repeated orf gene (bro-p). These deletions were not seen in the LdMNPV-45/0 strain nor in other American strains available in archiving systems. We also found deletions of the bro-e and bro-o genes in LdMNPV-45/0 strain but not in the LdMNPV-27/0 strain. The phylogenetic inference with an alignment of the 37 core gene nucleotide sequences revealed the close relationship of the LdMNPV-45/0 strain with other American strains accessed in GenBank (Ab-a624 and 5-6) while the LdMNPV-27/0 strain was clustered together with the LdMNPV-3054 strain (isolated in Spain) instead of predicted clustering with LdMNPV- 3029 (isolated in Asia). Our study demonstrated that first, different LdMNPV isolates from the same metapopulations of L. dispar exhibit little or no difference in the degree of virulence towards host larvae and second, that locality of host population is not an important driver of LdMNPV virulence. Virulence of LdMNPV is determined only by viral genetics. The genetic differences between North American and Central Asian virus strains are discussed.
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Complete Genome Sequence of a Western Siberian Lymantria dispar Multiple Nucleopolyhedrovirus Isolate
Genome Announcements, 2015Co-Authors: Marsel R. Kabilov, Alexander Bondar, Olga A Baturina, V V Martemyanov, Aleksey E. Tupikin, Irina A. Belousova, Alexandr V. IlyinykhAbstract:ABSTRACT A novel strain of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV-27) was isolated from dead larvae of a Western Siberian (WS) population of gypsy moths (Lymantria dispar L.). We report the complete genome sequence of this strain, comprising 164,108 bp and double-stranded circular DNA encoding 162 predicted open reading frames.
Martin Shapiro - One of the best experts on this subject based on the ideXlab platform.
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eicosanoid biosynthesis inhibitors increase the susceptibility of Lymantria dispar to nucleopolyhedrovirus ldmnpv
Journal of Invertebrate Pathology, 2007Co-Authors: David Stanley, Martin ShapiroAbstract:Eighteen pharmaceutical inhibitors of eicosanoid biosynthesis were tested for their effects on gypsy moth, Lymantria dispar and its susceptibility to the nucleopoly-hedrovirus LdMNPV. None of the inhibitors tested had any detrimental effects upon larval growth and development. Treatment with nine inhibitor/NPV combinations (e.g., bromophenacylbromide, clotrimazole, dexamethasone, esculetin, flufenamic acid, indomethacin, nimesulide, sulindac, tolfenamic acid) resulted in 3.5- to 6.6-fold reductions in LC50s. Larvae treated with several other COX inhibitors did not yield significant LC50 reductions. We infer that eicosanoids act in insect defense responses to viral infection. Eicosanoids may act at three levels of insect immune reactions to viral infection, organismal (febrile response), cellular (hemocytic microaggregation, nodulation and plasmatocytes spreading reactions) and intracellular level (mechanisms responsible for insect permissiveness to viral replication).
