The Experts below are selected from a list of 16164 Experts worldwide ranked by ideXlab platform
Allan Stensballe - One of the best experts on this subject based on the ideXlab platform.
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induction of a regulatory phenotype in cd3 cd4 hla dr t cells after allogeneic mixed Lymphocyte Culture indications of both contact dependent and independent activation
International Journal of Molecular Sciences, 2017Co-Authors: Anne Louise Revenfeld, Rikke Baek, Malene Jorgensen, Kim Varming, Allan StensballeAbstract:Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed Lymphocyte Culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication.
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Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation
MDPI AG, 2017Co-Authors: Anne Louise Revenfeld, Rikke Baek, Kim Varming, Malene Møller Jørgensen, Allan StensballeAbstract:Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed Lymphocyte Culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication
Arndt Borkhardt - One of the best experts on this subject based on the ideXlab platform.
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cytokine release patterns in mixed Lymphocyte Culture mlc of t cells with dendritic cells dc generated from aml blasts contribute to predict anti leukaemic t cell reactions and patients response to immunotherapy
Cell Communication and Adhesion, 2015Co-Authors: Dorothea Fischbacher, Marion Merle, Anja Liepert, Christine Grabrucker, Tanja Kroell, Andreas Kremser, Julia Dreysig, Markus Freudenreich, Friedhelm R Schuster, Arndt BorkhardtAbstract:AbstractTo enlighten interactions between autologous, allogeneic or T-cells from patients after stem cell transplantation with leukaemia-derived-dendritic-cells containing dendritic cells or blast containing mononuclear cells (n = 21, respectively), we determined cytokine-concentrations (interleukin 2, 4, 6, 10, tumor-necrosis-factor-α, interferon-γ) in supernatants of mixed-Lymphocyte-Culture and in serum (n = 16) of 20 patients with acute myeloid leukaemia and three patients with myelodysplastic syndromes by cytometric-bead-assay. We correlated our data with lytic capabilities of stimulated T-cells in a fluorolysis-assay and clinical data:Dendritic-cell-/mononuclear-cell-stimulation of T-cells resulted in increased cytokine-levels in Culture-medium compared to serum. There were no significant differences between cytokine-patterns of cases with/without lytic T-cell-activity, response to immunotherapy (stem cell transplantation/donor-Lymphocyte-infusion) or graft-versus-host-disease. However, some predict...
Lothar Rink - One of the best experts on this subject based on the ideXlab platform.
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interferon gamma plays a key role in the human mixed Lymphocyte Culture
Bone Marrow Transplantation, 1996Co-Authors: Susanne G Danzer, C Acampo, Lothar RinkAbstract:Measurement of cytokines in the mixed Lymphocyte Culture (MLC) is thought to be a new and relevant parameter for bone marrow transplantation (BMT). Our experiments showed that IFN-gamma plays a central role in the cytokine network following alloantigenic recognition. IFN-gamma itself is induced by IL-2 since anti-IL-2 strongly reduced the secretion of IFN-gamma. As anti-IFN-gamma also diminished the response of IL-2 and sIL-2R, a feedback mechanism between these two cytokines is assumed. Addition of rIFN-gamma to the MLC augmented the release of sCD8 molecules, whereas sCD4 molecules were reduced, indicating that IFN-gamma led to T cell differentiation instead of IL-2 dependent proliferation. In the MLC, a feedback mechanism between TNF-alpha and IFN-gamma exists, since anti-TNF-gamma reduced the secretion of IFN-gamma and anti-IFN-gamma inhibited the release of TNF-alpha. Therefore, IFN-gamma plays a critical role in monocyte activation, T cell differentiation, and IL-2-induced cell growth. We conclude that measurement of IFN-gamma might be a new and more sensitive parameter for BMT than the established proliferation assay, since IFN-gamma directly quantifies T cell activation.
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cytokine interactions in human mixed Lymphocyte Culture
Transplantation, 1994Co-Authors: Susanne G Danzer, Holger Kirchner, Lothar RinkAbstract:Human PBMC of healthy blood donors were used to investigate the interaction of cytokines in human MLC. Two-way MLC was performed because irradiation did not influence the cytokine release. We found different kinetic patterns for IL-2, IFN-gamma, and sIL-2R. Production of IFN-gamma was dependent on IL-2 release because anti-IL-2 addition resulted in more than 90% reduced IFN-gamma levels. Treatment with rIL-2 altered IFN-gamma kinetics, but not the total amount of IFN-gamma. Addition of anti-IFN-gamma led to decreased production of IL-2 and sIL-2R. A down-regulation of IL-2 and sIL-2R could also be observed after treatment with rIFN-gamma. No production of IL-4 and IL-10 was detected in MLC (detection limit 5 pg/ml). This could not be explained by IFN-gamma antagonism because IL-4 and IL-10 were not detectable even after addition of anti-IFN-gamma. Testing the TH1-TH2 cell antagonism, the addition of rIL-4 and rIL-10 resulted in IFN-gamma suppression depending on the timing of exposition. Treatment with rIL-10 inhibited IL-2 and sIL-2R release. We found no production of IL-1 alpha and IL-1 beta in MLC, whereas IL-6 and TNF-alpha release could be detected. Surprisingly, release of IL-6 and TNF-alpha could be blocked completely by addition of anti-IFN-gamma. This suggests that the release of IL-6 and TNF-alpha in MLC is dependent on IFN-gamma produced by T cells. In summary, TH1 cytokines play a central role in MLC regulation, whereas TH2 cytokines appear to be of little importance.
Anne Louise Revenfeld - One of the best experts on this subject based on the ideXlab platform.
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induction of a regulatory phenotype in cd3 cd4 hla dr t cells after allogeneic mixed Lymphocyte Culture indications of both contact dependent and independent activation
International Journal of Molecular Sciences, 2017Co-Authors: Anne Louise Revenfeld, Rikke Baek, Malene Jorgensen, Kim Varming, Allan StensballeAbstract:Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed Lymphocyte Culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication.
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Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation
MDPI AG, 2017Co-Authors: Anne Louise Revenfeld, Rikke Baek, Kim Varming, Malene Møller Jørgensen, Allan StensballeAbstract:Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed Lymphocyte Culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication
Susanne G Danzer - One of the best experts on this subject based on the ideXlab platform.
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Dialysis
2016Co-Authors: Susanne G Danzer, L RinkAbstract:Reverse transcription polymerase chain reaction for cytokines in the mixed Lymphocyte Culture. Is there a role in renal transplantation
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interferon gamma plays a key role in the human mixed Lymphocyte Culture
Bone Marrow Transplantation, 1996Co-Authors: Susanne G Danzer, C Acampo, Lothar RinkAbstract:Measurement of cytokines in the mixed Lymphocyte Culture (MLC) is thought to be a new and relevant parameter for bone marrow transplantation (BMT). Our experiments showed that IFN-gamma plays a central role in the cytokine network following alloantigenic recognition. IFN-gamma itself is induced by IL-2 since anti-IL-2 strongly reduced the secretion of IFN-gamma. As anti-IFN-gamma also diminished the response of IL-2 and sIL-2R, a feedback mechanism between these two cytokines is assumed. Addition of rIFN-gamma to the MLC augmented the release of sCD8 molecules, whereas sCD4 molecules were reduced, indicating that IFN-gamma led to T cell differentiation instead of IL-2 dependent proliferation. In the MLC, a feedback mechanism between TNF-alpha and IFN-gamma exists, since anti-TNF-gamma reduced the secretion of IFN-gamma and anti-IFN-gamma inhibited the release of TNF-alpha. Therefore, IFN-gamma plays a critical role in monocyte activation, T cell differentiation, and IL-2-induced cell growth. We conclude that measurement of IFN-gamma might be a new and more sensitive parameter for BMT than the established proliferation assay, since IFN-gamma directly quantifies T cell activation.
-
cytokine interactions in human mixed Lymphocyte Culture
Transplantation, 1994Co-Authors: Susanne G Danzer, Holger Kirchner, Lothar RinkAbstract:Human PBMC of healthy blood donors were used to investigate the interaction of cytokines in human MLC. Two-way MLC was performed because irradiation did not influence the cytokine release. We found different kinetic patterns for IL-2, IFN-gamma, and sIL-2R. Production of IFN-gamma was dependent on IL-2 release because anti-IL-2 addition resulted in more than 90% reduced IFN-gamma levels. Treatment with rIL-2 altered IFN-gamma kinetics, but not the total amount of IFN-gamma. Addition of anti-IFN-gamma led to decreased production of IL-2 and sIL-2R. A down-regulation of IL-2 and sIL-2R could also be observed after treatment with rIFN-gamma. No production of IL-4 and IL-10 was detected in MLC (detection limit 5 pg/ml). This could not be explained by IFN-gamma antagonism because IL-4 and IL-10 were not detectable even after addition of anti-IFN-gamma. Testing the TH1-TH2 cell antagonism, the addition of rIL-4 and rIL-10 resulted in IFN-gamma suppression depending on the timing of exposition. Treatment with rIL-10 inhibited IL-2 and sIL-2R release. We found no production of IL-1 alpha and IL-1 beta in MLC, whereas IL-6 and TNF-alpha release could be detected. Surprisingly, release of IL-6 and TNF-alpha could be blocked completely by addition of anti-IFN-gamma. This suggests that the release of IL-6 and TNF-alpha in MLC is dependent on IFN-gamma produced by T cells. In summary, TH1 cytokines play a central role in MLC regulation, whereas TH2 cytokines appear to be of little importance.
