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Dale L. Greiner - One of the best experts on this subject based on the ideXlab platform.
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Printed in the United States of America Rat Stem-Cell Factor Induces Splenocytes Capable of Regenerating the Thymus
2013Co-Authors: Eugene S. Medlock, Russell T T. Migita, Lisa D. Trebasky, Jerry M. Housmanfl, Gary S. Elliott, Wayne R. Hendren, Randolph B. Deprince, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that 164 recombinant rat SCF (rrSCF administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis
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rat stem Cell factor induces splenocytes capable of regenerating the thymus
Developmental Immunology, 1992Co-Authors: Eugene Medlock, Russell T T. Migita, Lisa D. Trebasky, Wayne R. Hendren, Randolph B. Deprince, Jerry M Housman, Gary Elliott, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that recombinant rat SCF (rrSCF164 administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis.
Tetsuo Tsukamoto - One of the best experts on this subject based on the ideXlab platform.
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Data_Sheet_1_HIV Impacts CD34+ Progenitors Involved in T-Cell Differentiation During Coculture With Mouse Stromal OP9-DL1 Cells.PDF
2019Co-Authors: Tetsuo TsukamotoAbstract:HIV-1 causes the loss of CD4+ T Cells via depletion or impairment of their production. The latter involves infection of thymocytes, but the involvement of hematopoietic CD34+ Cells remains unclear even though HIV-positive patients frequently manifest myelosuppression. In order to have a closer look at the impact of HIV-1 on T-lineage differentiation, this study utilized the OP9-DL1 coculture system, which supports in vitro T-lineage differentiation of human hematopoietic stem/Progenitor Cells. In the newly developed in vitro OP9-DL1/HIV-1 model, cord-derived CD34+ Cells were infected with CXCR4-tropic HIV-1NL4−3 and cocultured. The HIV-infected cocultures exhibited reduced CD4+ T-Cell growth at weeks 3–5 post infection compared to autologous uninfected cocultures. Further assays and analyses revealed that CD34+CD7+CXCR4+ Cells can be quickly depleted as early as 1 week after infection of the subset, and this was accompanied by the emergence of rare CD34+CD7+CD4+ Cells. A subsequent theoretical model analysis suggested potential influence of HIV-1 on the differentiation rate or death rate of Lymphoid Progenitor Cells. These results indicate that CXCR4-tropic HIV-1 strains may impact the dynamics of CD34+CD7+ Lymphoid Progenitor Cell pools, presumably leading to impaired T-Cell production potential.
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HIV Impacts CD34+ Progenitors Involved in T-Cell Differentiation During Coculture With Mouse Stromal OP9-DL1 Cells
'Frontiers Media SA', 2019Co-Authors: Tetsuo TsukamotoAbstract:HIV-1 causes the loss of CD4+ T Cells via depletion or impairment of their production. The latter involves infection of thymocytes, but the involvement of hematopoietic CD34+ Cells remains unclear even though HIV-positive patients frequently manifest myelosuppression. In order to have a closer look at the impact of HIV-1 on T-lineage differentiation, this study utilized the OP9-DL1 coculture system, which supports in vitro T-lineage differentiation of human hematopoietic stem/Progenitor Cells. In the newly developed in vitro OP9-DL1/HIV-1 model, cord-derived CD34+ Cells were infected with CXCR4-tropic HIV-1NL4−3 and cocultured. The HIV-infected cocultures exhibited reduced CD4+ T-Cell growth at weeks 3–5 post infection compared to autologous uninfected cocultures. Further assays and analyses revealed that CD34+CD7+CXCR4+ Cells can be quickly depleted as early as 1 week after infection of the subset, and this was accompanied by the emergence of rare CD34+CD7+CD4+ Cells. A subsequent theoretical model analysis suggested potential influence of HIV-1 on the differentiation rate or death rate of Lymphoid Progenitor Cells. These results indicate that CXCR4-tropic HIV-1 strains may impact the dynamics of CD34+CD7+ Lymphoid Progenitor Cell pools, presumably leading to impaired T-Cell production potential
Eugene Medlock - One of the best experts on this subject based on the ideXlab platform.
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rat stem Cell factor induces splenocytes capable of regenerating the thymus
Developmental Immunology, 1992Co-Authors: Eugene Medlock, Russell T T. Migita, Lisa D. Trebasky, Wayne R. Hendren, Randolph B. Deprince, Jerry M Housman, Gary Elliott, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that recombinant rat SCF (rrSCF164 administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis.
Russell T T. Migita - One of the best experts on this subject based on the ideXlab platform.
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Printed in the United States of America Rat Stem-Cell Factor Induces Splenocytes Capable of Regenerating the Thymus
2013Co-Authors: Eugene S. Medlock, Russell T T. Migita, Lisa D. Trebasky, Jerry M. Housmanfl, Gary S. Elliott, Wayne R. Hendren, Randolph B. Deprince, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that 164 recombinant rat SCF (rrSCF administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis
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rat stem Cell factor induces splenocytes capable of regenerating the thymus
Developmental Immunology, 1992Co-Authors: Eugene Medlock, Russell T T. Migita, Lisa D. Trebasky, Wayne R. Hendren, Randolph B. Deprince, Jerry M Housman, Gary Elliott, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that recombinant rat SCF (rrSCF164 administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis.
Randolph B. Deprince - One of the best experts on this subject based on the ideXlab platform.
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Printed in the United States of America Rat Stem-Cell Factor Induces Splenocytes Capable of Regenerating the Thymus
2013Co-Authors: Eugene S. Medlock, Russell T T. Migita, Lisa D. Trebasky, Jerry M. Housmanfl, Gary S. Elliott, Wayne R. Hendren, Randolph B. Deprince, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that 164 recombinant rat SCF (rrSCF administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis
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rat stem Cell factor induces splenocytes capable of regenerating the thymus
Developmental Immunology, 1992Co-Authors: Eugene Medlock, Russell T T. Migita, Lisa D. Trebasky, Wayne R. Hendren, Randolph B. Deprince, Jerry M Housman, Gary Elliott, Dale L. GreinerAbstract:Cytokine regulation of prethymic T-Lymphoid Progenitor-Cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and Progenitor-Cell development. Here we use a recently identified hemopoietic growth factor, stem-Cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte Progenitor-Cell activity. We show that recombinant rat SCF (rrSCF164 administered to weanling rats selectively induces an increase in thymocyte Progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating Cells and may influence, directly or indirectly, early prethymic stages of T-Cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis.
