The Experts below are selected from a list of 7242 Experts worldwide ranked by ideXlab platform
Phillip I Tarr - One of the best experts on this subject based on the ideXlab platform.
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shiga toxin antigen detection should not replace sorbitol MacConkey Agar screening of stool specimens
Journal of Clinical Microbiology, 2004Co-Authors: Eileen J Klein, Jennifer R Stapp, Marguerite A Neill, John M Besser, Michael T Osterholm, Phillip I Tarr, Richard B Thomson, Patrick J Gavin, Lance R PetersonAbstract:The interesting paper by Gavin et al., describing their experience using an enzyme immunoassay (EIA) to identify stools containing Shiga toxin (Stx)-producing Escherichia coli (STEC) (3), adds to the growing literature that non-O157:H7 STEC are overlooked pathogens. However, as physicians, clinical and public health microbiologists, and disease control epidemiologists, we are concerned by the authors' use of an EIA to screen stools and, only if this test is positive, to then seek E. coli O157:H7 with sorbitol MacConkey Agar culture (SMAC).
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comparison of escherichia coli o157 h7 antigen detection in stool and broth cultures to that in sorbitol MacConkey Agar stool cultures
Journal of Clinical Microbiology, 2000Co-Authors: Jennifer R Stapp, Eileen J Klein, Srdjan Jelacic, Yoolee Yea, Marc Fischer, Carla R Clausen, Xuan Qin, David L Swerdlow, Phillip I TarrAbstract:We evaluated the Meridian IC-STAT direct fecal and broth culture antigen detection methods with samples from children infected with Escherichia coli O157:H7 and correlated the antigen detection results with the culture results. Stools of 16 children who had recently had stool cultures positive for this pathogen (population A) and 102 children with diarrhea of unknown cause (population B) were tested with the IC-STAT device (direct testing). Fecal broth cultures were also tested with this device (broth testing). The results were correlated to a standard of the combined yield from direct culture of stools on sorbitol-MacConkey (SMAC) Agar and culture of broth on SMAC Agar. Eleven (69%) of the population A stool specimens yielded E. coli O157:H7 when plated directly on SMAC Agar. Two more specimens yielded this pathogen when the broth culture was similarly plated. Of these 13 stool specimens, 8 and 13 were positive by direct and broth testing (respective sensitivities, 62 and 100%). Compared to the sensitivity of a simultaneously performed SMAC Agar culture, the sensitivity of direct testing was 73%. Three (3%) of the population B stool specimens contained E. coli O157:H7 on SMAC Agar culture; one and three of these stool specimens were positive by direct and broth testing, respectively. The direct and broth IC-STAT tests were 100% specific with samples from children from population B. Direct IC-STAT testing of stools is rapid, easily performed, and specific but is insufficiently sensitive to exclude the possibility of infection with E. coli O157:H7. Performing the IC-STAT test with a broth culture increases its sensitivity. However, attempts to recover E. coli O157:H7 by culture should not be abandoned but, rather, should be increased when the IC-STAT test result is positive.
Eileen J Klein - One of the best experts on this subject based on the ideXlab platform.
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shiga toxin antigen detection should not replace sorbitol MacConkey Agar screening of stool specimens
Journal of Clinical Microbiology, 2004Co-Authors: Eileen J Klein, Jennifer R Stapp, Marguerite A Neill, John M Besser, Michael T Osterholm, Phillip I Tarr, Richard B Thomson, Patrick J Gavin, Lance R PetersonAbstract:The interesting paper by Gavin et al., describing their experience using an enzyme immunoassay (EIA) to identify stools containing Shiga toxin (Stx)-producing Escherichia coli (STEC) (3), adds to the growing literature that non-O157:H7 STEC are overlooked pathogens. However, as physicians, clinical and public health microbiologists, and disease control epidemiologists, we are concerned by the authors' use of an EIA to screen stools and, only if this test is positive, to then seek E. coli O157:H7 with sorbitol MacConkey Agar culture (SMAC).
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comparison of escherichia coli o157 h7 antigen detection in stool and broth cultures to that in sorbitol MacConkey Agar stool cultures
Journal of Clinical Microbiology, 2000Co-Authors: Jennifer R Stapp, Eileen J Klein, Srdjan Jelacic, Yoolee Yea, Marc Fischer, Carla R Clausen, Xuan Qin, David L Swerdlow, Phillip I TarrAbstract:We evaluated the Meridian IC-STAT direct fecal and broth culture antigen detection methods with samples from children infected with Escherichia coli O157:H7 and correlated the antigen detection results with the culture results. Stools of 16 children who had recently had stool cultures positive for this pathogen (population A) and 102 children with diarrhea of unknown cause (population B) were tested with the IC-STAT device (direct testing). Fecal broth cultures were also tested with this device (broth testing). The results were correlated to a standard of the combined yield from direct culture of stools on sorbitol-MacConkey (SMAC) Agar and culture of broth on SMAC Agar. Eleven (69%) of the population A stool specimens yielded E. coli O157:H7 when plated directly on SMAC Agar. Two more specimens yielded this pathogen when the broth culture was similarly plated. Of these 13 stool specimens, 8 and 13 were positive by direct and broth testing (respective sensitivities, 62 and 100%). Compared to the sensitivity of a simultaneously performed SMAC Agar culture, the sensitivity of direct testing was 73%. Three (3%) of the population B stool specimens contained E. coli O157:H7 on SMAC Agar culture; one and three of these stool specimens were positive by direct and broth testing, respectively. The direct and broth IC-STAT tests were 100% specific with samples from children from population B. Direct IC-STAT testing of stools is rapid, easily performed, and specific but is insufficiently sensitive to exclude the possibility of infection with E. coli O157:H7. Performing the IC-STAT test with a broth culture increases its sensitivity. However, attempts to recover E. coli O157:H7 by culture should not be abandoned but, rather, should be increased when the IC-STAT test result is positive.
Jennifer R Stapp - One of the best experts on this subject based on the ideXlab platform.
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shiga toxin antigen detection should not replace sorbitol MacConkey Agar screening of stool specimens
Journal of Clinical Microbiology, 2004Co-Authors: Eileen J Klein, Jennifer R Stapp, Marguerite A Neill, John M Besser, Michael T Osterholm, Phillip I Tarr, Richard B Thomson, Patrick J Gavin, Lance R PetersonAbstract:The interesting paper by Gavin et al., describing their experience using an enzyme immunoassay (EIA) to identify stools containing Shiga toxin (Stx)-producing Escherichia coli (STEC) (3), adds to the growing literature that non-O157:H7 STEC are overlooked pathogens. However, as physicians, clinical and public health microbiologists, and disease control epidemiologists, we are concerned by the authors' use of an EIA to screen stools and, only if this test is positive, to then seek E. coli O157:H7 with sorbitol MacConkey Agar culture (SMAC).
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comparison of escherichia coli o157 h7 antigen detection in stool and broth cultures to that in sorbitol MacConkey Agar stool cultures
Journal of Clinical Microbiology, 2000Co-Authors: Jennifer R Stapp, Eileen J Klein, Srdjan Jelacic, Yoolee Yea, Marc Fischer, Carla R Clausen, Xuan Qin, David L Swerdlow, Phillip I TarrAbstract:We evaluated the Meridian IC-STAT direct fecal and broth culture antigen detection methods with samples from children infected with Escherichia coli O157:H7 and correlated the antigen detection results with the culture results. Stools of 16 children who had recently had stool cultures positive for this pathogen (population A) and 102 children with diarrhea of unknown cause (population B) were tested with the IC-STAT device (direct testing). Fecal broth cultures were also tested with this device (broth testing). The results were correlated to a standard of the combined yield from direct culture of stools on sorbitol-MacConkey (SMAC) Agar and culture of broth on SMAC Agar. Eleven (69%) of the population A stool specimens yielded E. coli O157:H7 when plated directly on SMAC Agar. Two more specimens yielded this pathogen when the broth culture was similarly plated. Of these 13 stool specimens, 8 and 13 were positive by direct and broth testing (respective sensitivities, 62 and 100%). Compared to the sensitivity of a simultaneously performed SMAC Agar culture, the sensitivity of direct testing was 73%. Three (3%) of the population B stool specimens contained E. coli O157:H7 on SMAC Agar culture; one and three of these stool specimens were positive by direct and broth testing, respectively. The direct and broth IC-STAT tests were 100% specific with samples from children from population B. Direct IC-STAT testing of stools is rapid, easily performed, and specific but is insufficiently sensitive to exclude the possibility of infection with E. coli O157:H7. Performing the IC-STAT test with a broth culture increases its sensitivity. However, attempts to recover E. coli O157:H7 by culture should not be abandoned but, rather, should be increased when the IC-STAT test result is positive.
Lance R Peterson - One of the best experts on this subject based on the ideXlab platform.
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shiga toxin antigen detection should not replace sorbitol MacConkey Agar screening of stool specimens
Journal of Clinical Microbiology, 2004Co-Authors: Eileen J Klein, Jennifer R Stapp, Marguerite A Neill, John M Besser, Michael T Osterholm, Phillip I Tarr, Richard B Thomson, Patrick J Gavin, Lance R PetersonAbstract:The interesting paper by Gavin et al., describing their experience using an enzyme immunoassay (EIA) to identify stools containing Shiga toxin (Stx)-producing Escherichia coli (STEC) (3), adds to the growing literature that non-O157:H7 STEC are overlooked pathogens. However, as physicians, clinical and public health microbiologists, and disease control epidemiologists, we are concerned by the authors' use of an EIA to screen stools and, only if this test is positive, to then seek E. coli O157:H7 with sorbitol MacConkey Agar culture (SMAC).
Mark D Sobsey - One of the best experts on this subject based on the ideXlab platform.
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development of a l rhamnose and d arabitol supplemented MacConkey Agar to identify pathogenic yersinia enterocolitica among environmental yersinias in swine production wastes
Journal of Microbiological Methods, 2004Co-Authors: M W Shehee, Mark D SobseyAbstract:Supplemented MacConkey Agar with l-rhamnose and d-arabitol distinguishes pathogenic from environmental strains of Yersinia enterocolitica recovered from swine production wastes. This medium has a sensitivity of 100% and a specificity of 97.4%.
