The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform
Kousaku Murata - One of the best experts on this subject based on the ideXlab platform.
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special cell surface structure and novel Macromolecule Transport depolymerization system of sphingomonas sp a1
Journal of Industrial Microbiology & Biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Hyejin Yoon, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
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Special cell surface structure, and novel Macromolecule Transport/depolymerization system of Sphingomonas sp A1.
Journal of industrial microbiology & biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, H-j Yoon, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
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Pit structure on bacterial cell surface.
Biochemical and Biophysical Research Communications, 1996Co-Authors: Tomohiro Hisano, Wataru Hashimoto, Nobuko Kimura, Kousaku MurataAbstract:Abstract The yellow-pigmented bacterium isolated from a ditch was a Gram negative rod with a G+C content of 63 mol%, and was classified in the genus Sphingomonas. Electron microscopy revealed that the bacterial cell surface was covered with many large plaits. When grown in a medium containing a polysaccharide as an essential nutrient, a pit of 0.02∼0.1 μm in diameter was formed on the cell surface, and a thin section showed the rearrangement of the plaits and the presence of a region where the cell membrane sinks into the cytosol. The dependence of the pit formation on the presence of Macromolecule may predict the existence of a direct uptake mechanism for Macromolecules through a mouth-like pit, possibly in endocytosis fashion. The confirmation of the pit structure is the first such finding in the history of microbiology and may provide a new insight into the cell morphology and biochemistry of Macromolecule Transport in microbial cell system.
Mauro Ferrari - One of the best experts on this subject based on the ideXlab platform.
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tumor vascular permeabilization using localized mild hyperthermia to improve Macromolecule Transport
Nanomedicine: Nanotechnology Biology and Medicine, 2014Co-Authors: Dickson K Kirui, Eugene J Koay, Vittorio Cristini, Haifa Shen, Mauro FerrariAbstract:Abstract The abnormal tumor vasculature presents a major challenge to the adequate delivery of chemotherapeutics, often limiting efficacy. We developed a nanoparticle-based technique to deliver localized mild hyperthermia (MHT) used to transiently alter tumor vascular Transport properties and enhance Transport of Macromolecules into tumor interstitium. The strategy involved administering and localizing accumulation of stealth gold nanorods (GNRs, 103μg of GNRs/g of tumor), and irradiating tumor with a low-photon laser flux (1W/cm 2 ) to generate MHT. The treatment increased vascular permeability within 24h after treatment, allowing enhanced Transport of Macromolecules up to 54nm in size. A mathematical model is used to describe changes in tumor mass Transport properties where the rate of macromolecular exchange between interstitial and vascular region (R) and maximum dye enhancement (Y max ) of 23-nm dextran dye is analytically solved. During enhanced permeability, R increased by 200% while Y max increased by 30% relative to untreated group in pancreatic CAPAN-1 tumors. MHT treatment also enhanced Transport of larger dextran dye (54nm) as assessed by intravital microscopy, without causing occlusive cellular damage. Enhanced vascular Transport was prolonged for up to 24h after treatment, but reversible with Transport parameters returning to basal levels after 36h. This study indicates that localized mild hyperthermia treatment opens a transient time-window with which to enable and augment Macromolecule Transport and potentially improve therapeutic efficacy. From the Clinical Editor In this study, local intra-tumor mild hyperthermia is induced using a nanoparticle-based approach utilizing stealth gold nanorods and irradiating the tumor with low-photon laser flux, resulting in locally increased vascular permeability enabling enhanced delivery of therapeutics, including Macromolecules up to 54nm in size. Similar approaches would be very helpful in addressing treatment-resistant malignancies in clinical practice.
Keiko Momma - One of the best experts on this subject based on the ideXlab platform.
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special cell surface structure and novel Macromolecule Transport depolymerization system of sphingomonas sp a1
Journal of Industrial Microbiology & Biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Hyejin Yoon, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
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Special cell surface structure, and novel Macromolecule Transport/depolymerization system of Sphingomonas sp A1.
Journal of industrial microbiology & biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, H-j Yoon, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
Wataru Hashimoto - One of the best experts on this subject based on the ideXlab platform.
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special cell surface structure and novel Macromolecule Transport depolymerization system of sphingomonas sp a1
Journal of Industrial Microbiology & Biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Hyejin Yoon, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
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Special cell surface structure, and novel Macromolecule Transport/depolymerization system of Sphingomonas sp A1.
Journal of industrial microbiology & biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, H-j Yoon, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
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Pit structure on bacterial cell surface.
Biochemical and Biophysical Research Communications, 1996Co-Authors: Tomohiro Hisano, Wataru Hashimoto, Nobuko Kimura, Kousaku MurataAbstract:Abstract The yellow-pigmented bacterium isolated from a ditch was a Gram negative rod with a G+C content of 63 mol%, and was classified in the genus Sphingomonas. Electron microscopy revealed that the bacterial cell surface was covered with many large plaits. When grown in a medium containing a polysaccharide as an essential nutrient, a pit of 0.02∼0.1 μm in diameter was formed on the cell surface, and a thin section showed the rearrangement of the plaits and the presence of a region where the cell membrane sinks into the cytosol. The dependence of the pit formation on the presence of Macromolecule may predict the existence of a direct uptake mechanism for Macromolecules through a mouth-like pit, possibly in endocytosis fashion. The confirmation of the pit structure is the first such finding in the history of microbiology and may provide a new insight into the cell morphology and biochemistry of Macromolecule Transport in microbial cell system.
Yumiko Mishima - One of the best experts on this subject based on the ideXlab platform.
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special cell surface structure and novel Macromolecule Transport depolymerization system of sphingomonas sp a1
Journal of Industrial Microbiology & Biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Hyejin Yoon, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
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Special cell surface structure, and novel Macromolecule Transport/depolymerization system of Sphingomonas sp A1.
Journal of industrial microbiology & biotechnology, 1999Co-Authors: Keiko Momma, Wataru Hashimoto, Osamu Miyake, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, H-j Yoon, Kousaku MurataAbstract:A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and Macromolecule-specific ABC Transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for Macromolecules.
