The Experts below are selected from a list of 3837 Experts worldwide ranked by ideXlab platform
Jeffrey L Spees - One of the best experts on this subject based on the ideXlab platform.
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transplantation of nonhematopoietic adult bone marrow stem progenitor Cells isolated by p75 nerve growth factor receptor into the penis rescues erectile function in a rat model of cavernous nerve injury
The Journal of Urology, 2010Co-Authors: Muammer Kendirci, Landon Trost, Benjamin Bakondi, Mandolin J Whitney, Wayne J G Hellstrom, Jeffrey L SpeesAbstract:Purpose: Radical prostatectomy for prostate cancer frequently results in erectile dysfunction and decreased quality of life. We investigated the effects of transplanting nonhematopoietic adult bone marrow stem/progenitor Cells (multipotent stromal Cells) into the corpus cavernosum in a rat model of bilateral cavernous nerve crush injury.Materials and Methods: Multipotent stromal Cells were isolated from the bone marrow of transgenic green fluorescent protein rats by plastic adherence (rat multipotent stromal Cells) or magnetic activated Cell Sorting using antibodies against p75 low affinity nerve growth factor receptor (p75 derived multipotent stromal Cells). Bilateral cavernous nerve crush injury was induced in adult male Sprague-Dawley® rats. Immediately after injury 8 rats each were injected intracavernously with phosphate buffered saline (vehicle control), fibroblasts (Cell control), rat multipotent stromal Cells (Cell treatment) or p75 derived multipotent stromal Cells (Cell treatment). Another 8 rat...
Vibhav Gautam - One of the best experts on this subject based on the ideXlab platform.
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Recent technological advancements in stem Cell research for targeted therapeutics
Drug Delivery and Translational Research, 2020Co-Authors: Anurag Kumar Singh, Santosh Kumar Singh, Bhaskar Gaurishankar, Swapnil C. Kamble, Pradeep Mishra, Deepak Kotiya, Suvakanta Barik, Neelam Atri, Vibhav GautamAbstract:Stem Cells have characteristic features of self-renewal, pluripotency and differentiation, which are responsible for replenishment of tissue or organ. Stem Cells are potentiated as therapeutic tool in drug targeting and regenerative medicine—from curing various neurological diseases and malignancies to congenital diseases. These technological advancements have established stem Cells as future of medicine. However, due to ethico-social limitations, the use of embryonic stem Cells (ESCs) has been avoided, while physiological availability of adult stem Cells (ASCs) and induced pluripotent stem Cells (iPSCs) has gained appropriate preference. These iPSCs are very much similar to ESCs in terms of their self-renewal and pluripotency. Here, we have summarized the technologies that have established stem Cells isolation, their molecular marker and factors responsible for their maintenance. Different Cellular (transcription factors, regulatory proteins, miRNA like miRNA-296, miRNA-145, etc.) and extraCellular components transcend stem Cell fate. Their identification and characterization involve development and efficient utilization of tools like magnetic activated Cell Sorting (MACS) and fluorescence activated Cell Sorting (FACS). Some of the technologies have been patented and spin-off’s based on them have been commercialized. In conclusion, we present the future scope and possibilities that stem Cell technologies behold for us. Graphical abstract Pictorial representation of therapeutic approaches for disease treatment using stem Cell technology. Disease-specific adult stem Cells are isolated along with niche Cells by utilizing tools like FACS/MACS/LCM, etc. Thereafter, Cells are reprogrammed through introduction of Yamanaka factors (Oct3/4, Sox2, c-myc, Klf4) to make induced pluripotent stem Cell (iPSCs). The disease-specific iPSCs undergo genetic modification after delivery of therapeutic gene through retroviral vehicle. The genetically modified Cells are introduced back in person with disease for therapeutic effects. FACS, fluorescence activated Cell Sorting; MACS, Magnetic-Activated Cell Sorting; LCM, laser capture microdissection; Oct3/4, octamer-binding transcription factor 3/4; Sox2, sex determining region Y-box 2; Klf4, Kruppel-like factor 4.
Anurag Kumar Singh - One of the best experts on this subject based on the ideXlab platform.
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Recent technological advancements in stem Cell research for targeted therapeutics
Drug Delivery and Translational Research, 2020Co-Authors: Anurag Kumar Singh, Santosh Kumar Singh, Bhaskar Gaurishankar, Swapnil C. Kamble, Pradeep Mishra, Deepak Kotiya, Suvakanta Barik, Neelam Atri, Vibhav GautamAbstract:Stem Cells have characteristic features of self-renewal, pluripotency and differentiation, which are responsible for replenishment of tissue or organ. Stem Cells are potentiated as therapeutic tool in drug targeting and regenerative medicine—from curing various neurological diseases and malignancies to congenital diseases. These technological advancements have established stem Cells as future of medicine. However, due to ethico-social limitations, the use of embryonic stem Cells (ESCs) has been avoided, while physiological availability of adult stem Cells (ASCs) and induced pluripotent stem Cells (iPSCs) has gained appropriate preference. These iPSCs are very much similar to ESCs in terms of their self-renewal and pluripotency. Here, we have summarized the technologies that have established stem Cells isolation, their molecular marker and factors responsible for their maintenance. Different Cellular (transcription factors, regulatory proteins, miRNA like miRNA-296, miRNA-145, etc.) and extraCellular components transcend stem Cell fate. Their identification and characterization involve development and efficient utilization of tools like magnetic activated Cell Sorting (MACS) and fluorescence activated Cell Sorting (FACS). Some of the technologies have been patented and spin-off’s based on them have been commercialized. In conclusion, we present the future scope and possibilities that stem Cell technologies behold for us. Graphical abstract Pictorial representation of therapeutic approaches for disease treatment using stem Cell technology. Disease-specific adult stem Cells are isolated along with niche Cells by utilizing tools like FACS/MACS/LCM, etc. Thereafter, Cells are reprogrammed through introduction of Yamanaka factors (Oct3/4, Sox2, c-myc, Klf4) to make induced pluripotent stem Cell (iPSCs). The disease-specific iPSCs undergo genetic modification after delivery of therapeutic gene through retroviral vehicle. The genetically modified Cells are introduced back in person with disease for therapeutic effects. FACS, fluorescence activated Cell Sorting; MACS, Magnetic-Activated Cell Sorting; LCM, laser capture microdissection; Oct3/4, octamer-binding transcription factor 3/4; Sox2, sex determining region Y-box 2; Klf4, Kruppel-like factor 4.
Christof Dörfer - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^− Cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, gingival margin-derived stem/progenitor Cells isolated via STRO-1/magnetic activated Cell Sorting (MACS) showed remarkable periodontal regenerative potential in vivo . As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor Cell characteristics of sorted STRO-1-positive (MACS^+) and STRO-1-negative (MACS^−) Cell populations from the human free gingival margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS^+ and MACS^− Cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both Cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS^+ and MACS^− Cell fractions showed plastic adherence. MACS^+ Cells, in contrast to MACS^− Cells, showed all of the predefined mesenchymal stem/progenitor Cell characteristics and a significantly higher number of CFUs ( P
Deepak Kotiya - One of the best experts on this subject based on the ideXlab platform.
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Recent technological advancements in stem Cell research for targeted therapeutics
Drug Delivery and Translational Research, 2020Co-Authors: Anurag Kumar Singh, Santosh Kumar Singh, Bhaskar Gaurishankar, Swapnil C. Kamble, Pradeep Mishra, Deepak Kotiya, Suvakanta Barik, Neelam Atri, Vibhav GautamAbstract:Stem Cells have characteristic features of self-renewal, pluripotency and differentiation, which are responsible for replenishment of tissue or organ. Stem Cells are potentiated as therapeutic tool in drug targeting and regenerative medicine—from curing various neurological diseases and malignancies to congenital diseases. These technological advancements have established stem Cells as future of medicine. However, due to ethico-social limitations, the use of embryonic stem Cells (ESCs) has been avoided, while physiological availability of adult stem Cells (ASCs) and induced pluripotent stem Cells (iPSCs) has gained appropriate preference. These iPSCs are very much similar to ESCs in terms of their self-renewal and pluripotency. Here, we have summarized the technologies that have established stem Cells isolation, their molecular marker and factors responsible for their maintenance. Different Cellular (transcription factors, regulatory proteins, miRNA like miRNA-296, miRNA-145, etc.) and extraCellular components transcend stem Cell fate. Their identification and characterization involve development and efficient utilization of tools like magnetic activated Cell Sorting (MACS) and fluorescence activated Cell Sorting (FACS). Some of the technologies have been patented and spin-off’s based on them have been commercialized. In conclusion, we present the future scope and possibilities that stem Cell technologies behold for us. Graphical abstract Pictorial representation of therapeutic approaches for disease treatment using stem Cell technology. Disease-specific adult stem Cells are isolated along with niche Cells by utilizing tools like FACS/MACS/LCM, etc. Thereafter, Cells are reprogrammed through introduction of Yamanaka factors (Oct3/4, Sox2, c-myc, Klf4) to make induced pluripotent stem Cell (iPSCs). The disease-specific iPSCs undergo genetic modification after delivery of therapeutic gene through retroviral vehicle. The genetically modified Cells are introduced back in person with disease for therapeutic effects. FACS, fluorescence activated Cell Sorting; MACS, Magnetic-Activated Cell Sorting; LCM, laser capture microdissection; Oct3/4, octamer-binding transcription factor 3/4; Sox2, sex determining region Y-box 2; Klf4, Kruppel-like factor 4.