The Experts below are selected from a list of 2622 Experts worldwide ranked by ideXlab platform

Fei Ling - One of the best experts on this subject based on the ideXlab platform.

  • isolation of anti saprolegnia lignans from Magnolia Officinalis and sar evaluation of honokiol magnolol analogs
    Bioorganic & Medicinal Chemistry Letters, 2019
    Co-Authors: Yu-feng Shen, Gao-xue Wang, Fei Ling
    Abstract:

    Abstract To control the fish fungal pathogen Saprolegnia, the effects of the petroleum ether extracts of Magnolia Officinalis were evaluated by a rapeseed (Brassicanapus) microplate method in vitro. By loading on an open silica gel column and eluting with petroleum ether-ethyl acetate-methanol, honokiol (C18H18O2) and magnolol (C18H18O2) were isolated from Magnolia Officinalis. Saprolegnia parasitica growth was inhibited significantly when honokiol concentration was >8.0 mg/L, and magnolol concentration was >9.0 mg/L, with EC50 values of 4.38 and 4.92 mg/L, respectively. Six honokiol and magnolol derivatives were designed, synthesized and evaluated for their anti-Saprolegnia activity. According to the results, double bond and hydroxyl played an important role in inhibiting Saprolegnia. Mechanistically, through the scanning electron microscope observation, honokiol and magnolol could cause the Saprolegnia parasitica mycelium tegumental damage including intensive wrinkles and nodular structures. Moreover, compared to traditional drugs kresoxim-methyl (LC50 = 0.66 mg/L) and azoxystrobin (LC50 = 2.71 mg/L), honokiol and magnolol showed a lower detrimental effect on zebrafish, with the LC50 values of 6.00 and 7.28 mg/L at 48 h, respectively. Overall, honokiol and magnolol were promising lead compounds for the development of commercial drugs anti-Saprolegnia.

  • Isolation of anti-Saprolegnia lignans from Magnolia Officinalis and SAR evaluation of honokiol/magnolol analogs.
    Bioorganic & medicinal chemistry letters, 2018
    Co-Authors: Yu-feng Shen, Gao-xue Wang, Fei Ling
    Abstract:

    Abstract To control the fish fungal pathogen Saprolegnia, the effects of the petroleum ether extracts of Magnolia Officinalis were evaluated by a rapeseed (Brassicanapus) microplate method in vitro. By loading on an open silica gel column and eluting with petroleum ether-ethyl acetate-methanol, honokiol (C18H18O2) and magnolol (C18H18O2) were isolated from Magnolia Officinalis. Saprolegnia parasitica growth was inhibited significantly when honokiol concentration was >8.0 mg/L, and magnolol concentration was >9.0 mg/L, with EC50 values of 4.38 and 4.92 mg/L, respectively. Six honokiol and magnolol derivatives were designed, synthesized and evaluated for their anti-Saprolegnia activity. According to the results, double bond and hydroxyl played an important role in inhibiting Saprolegnia. Mechanistically, through the scanning electron microscope observation, honokiol and magnolol could cause the Saprolegnia parasitica mycelium tegumental damage including intensive wrinkles and nodular structures. Moreover, compared to traditional drugs kresoxim-methyl (LC50 = 0.66 mg/L) and azoxystrobin (LC50 = 2.71 mg/L), honokiol and magnolol showed a lower detrimental effect on zebrafish, with the LC50 values of 6.00 and 7.28 mg/L at 48 h, respectively. Overall, honokiol and magnolol were promising lead compounds for the development of commercial drugs anti-Saprolegnia.

Yu-feng Shen - One of the best experts on this subject based on the ideXlab platform.

  • isolation of anti saprolegnia lignans from Magnolia Officinalis and sar evaluation of honokiol magnolol analogs
    Bioorganic & Medicinal Chemistry Letters, 2019
    Co-Authors: Yu-feng Shen, Gao-xue Wang, Fei Ling
    Abstract:

    Abstract To control the fish fungal pathogen Saprolegnia, the effects of the petroleum ether extracts of Magnolia Officinalis were evaluated by a rapeseed (Brassicanapus) microplate method in vitro. By loading on an open silica gel column and eluting with petroleum ether-ethyl acetate-methanol, honokiol (C18H18O2) and magnolol (C18H18O2) were isolated from Magnolia Officinalis. Saprolegnia parasitica growth was inhibited significantly when honokiol concentration was >8.0 mg/L, and magnolol concentration was >9.0 mg/L, with EC50 values of 4.38 and 4.92 mg/L, respectively. Six honokiol and magnolol derivatives were designed, synthesized and evaluated for their anti-Saprolegnia activity. According to the results, double bond and hydroxyl played an important role in inhibiting Saprolegnia. Mechanistically, through the scanning electron microscope observation, honokiol and magnolol could cause the Saprolegnia parasitica mycelium tegumental damage including intensive wrinkles and nodular structures. Moreover, compared to traditional drugs kresoxim-methyl (LC50 = 0.66 mg/L) and azoxystrobin (LC50 = 2.71 mg/L), honokiol and magnolol showed a lower detrimental effect on zebrafish, with the LC50 values of 6.00 and 7.28 mg/L at 48 h, respectively. Overall, honokiol and magnolol were promising lead compounds for the development of commercial drugs anti-Saprolegnia.

  • Isolation of anti-Saprolegnia lignans from Magnolia Officinalis and SAR evaluation of honokiol/magnolol analogs.
    Bioorganic & medicinal chemistry letters, 2018
    Co-Authors: Yu-feng Shen, Gao-xue Wang, Fei Ling
    Abstract:

    Abstract To control the fish fungal pathogen Saprolegnia, the effects of the petroleum ether extracts of Magnolia Officinalis were evaluated by a rapeseed (Brassicanapus) microplate method in vitro. By loading on an open silica gel column and eluting with petroleum ether-ethyl acetate-methanol, honokiol (C18H18O2) and magnolol (C18H18O2) were isolated from Magnolia Officinalis. Saprolegnia parasitica growth was inhibited significantly when honokiol concentration was >8.0 mg/L, and magnolol concentration was >9.0 mg/L, with EC50 values of 4.38 and 4.92 mg/L, respectively. Six honokiol and magnolol derivatives were designed, synthesized and evaluated for their anti-Saprolegnia activity. According to the results, double bond and hydroxyl played an important role in inhibiting Saprolegnia. Mechanistically, through the scanning electron microscope observation, honokiol and magnolol could cause the Saprolegnia parasitica mycelium tegumental damage including intensive wrinkles and nodular structures. Moreover, compared to traditional drugs kresoxim-methyl (LC50 = 0.66 mg/L) and azoxystrobin (LC50 = 2.71 mg/L), honokiol and magnolol showed a lower detrimental effect on zebrafish, with the LC50 values of 6.00 and 7.28 mg/L at 48 h, respectively. Overall, honokiol and magnolol were promising lead compounds for the development of commercial drugs anti-Saprolegnia.

Gao-xue Wang - One of the best experts on this subject based on the ideXlab platform.

  • isolation of anti saprolegnia lignans from Magnolia Officinalis and sar evaluation of honokiol magnolol analogs
    Bioorganic & Medicinal Chemistry Letters, 2019
    Co-Authors: Yu-feng Shen, Gao-xue Wang, Fei Ling
    Abstract:

    Abstract To control the fish fungal pathogen Saprolegnia, the effects of the petroleum ether extracts of Magnolia Officinalis were evaluated by a rapeseed (Brassicanapus) microplate method in vitro. By loading on an open silica gel column and eluting with petroleum ether-ethyl acetate-methanol, honokiol (C18H18O2) and magnolol (C18H18O2) were isolated from Magnolia Officinalis. Saprolegnia parasitica growth was inhibited significantly when honokiol concentration was >8.0 mg/L, and magnolol concentration was >9.0 mg/L, with EC50 values of 4.38 and 4.92 mg/L, respectively. Six honokiol and magnolol derivatives were designed, synthesized and evaluated for their anti-Saprolegnia activity. According to the results, double bond and hydroxyl played an important role in inhibiting Saprolegnia. Mechanistically, through the scanning electron microscope observation, honokiol and magnolol could cause the Saprolegnia parasitica mycelium tegumental damage including intensive wrinkles and nodular structures. Moreover, compared to traditional drugs kresoxim-methyl (LC50 = 0.66 mg/L) and azoxystrobin (LC50 = 2.71 mg/L), honokiol and magnolol showed a lower detrimental effect on zebrafish, with the LC50 values of 6.00 and 7.28 mg/L at 48 h, respectively. Overall, honokiol and magnolol were promising lead compounds for the development of commercial drugs anti-Saprolegnia.

  • Isolation of anti-Saprolegnia lignans from Magnolia Officinalis and SAR evaluation of honokiol/magnolol analogs.
    Bioorganic & medicinal chemistry letters, 2018
    Co-Authors: Yu-feng Shen, Gao-xue Wang, Fei Ling
    Abstract:

    Abstract To control the fish fungal pathogen Saprolegnia, the effects of the petroleum ether extracts of Magnolia Officinalis were evaluated by a rapeseed (Brassicanapus) microplate method in vitro. By loading on an open silica gel column and eluting with petroleum ether-ethyl acetate-methanol, honokiol (C18H18O2) and magnolol (C18H18O2) were isolated from Magnolia Officinalis. Saprolegnia parasitica growth was inhibited significantly when honokiol concentration was >8.0 mg/L, and magnolol concentration was >9.0 mg/L, with EC50 values of 4.38 and 4.92 mg/L, respectively. Six honokiol and magnolol derivatives were designed, synthesized and evaluated for their anti-Saprolegnia activity. According to the results, double bond and hydroxyl played an important role in inhibiting Saprolegnia. Mechanistically, through the scanning electron microscope observation, honokiol and magnolol could cause the Saprolegnia parasitica mycelium tegumental damage including intensive wrinkles and nodular structures. Moreover, compared to traditional drugs kresoxim-methyl (LC50 = 0.66 mg/L) and azoxystrobin (LC50 = 2.71 mg/L), honokiol and magnolol showed a lower detrimental effect on zebrafish, with the LC50 values of 6.00 and 7.28 mg/L at 48 h, respectively. Overall, honokiol and magnolol were promising lead compounds for the development of commercial drugs anti-Saprolegnia.

Jin Tae Hong - One of the best experts on this subject based on the ideXlab platform.

  • A Comparison between Extract Products of Magnolia Officinalis on Memory Impairment and Amyloidogenesis in a Transgenic Mouse Model of Alzheimer’s Disease
    Biomolecules & therapeutics, 2012
    Co-Authors: Young-jung Lee, Dong-young Choi, Sang-bae Han, Young-hee Kim, Ki Ho Kim, Yeon Hee Seong, Jin Tae Hong
    Abstract:

    The components of Magnolia Officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities. These efficacies have been sold many products as nutritional supplement extracted from bark of Magnolia Officinalis. Thus, to assess and compare neuroprotective effect in the nutritional supplement (Magnolia ExtractTM, Health Freedom Nutrition LLC, USA) and our ethanol extract of Magnolia Officinalis (BioLand LTD, Korea), we investigated memorial improving and anti-Alzheimer’s disease effects of extract products of Magnolia Officinalis in a transgenic AD mice model. Oral pretreatment of two extract products of Magnolia Officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented Aβ accumulation in the brain of Tg2576 mice. In addition, extract products of Magnolia Officinalis also decreased expression of β-site APP cleaving enzyme 1 (BACE1), amyloid precursor protein (APP) and its product, C99. Although both two extract products of Magnolia Officinalis could show preventive effect of memorial dysfunction and Aβ accumulation, our ethanol extract of Magnolia Officinalis (BioLand LTD, Korea) could be more effective than Magnolia ExtractTM (Health Freedom Nutrition LLC, USA). Therefore, our results showed that extract products of Magnolia Officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating β-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

  • a comparison between extract products of Magnolia Officinalis on memory impairment and amyloidogenesis in a transgenic mouse model of alzheimer s disease
    Biomolecules & Therapeutics, 2012
    Co-Authors: Young-jung Lee, Dong-young Choi, Sang-bae Han, Young-hee Kim, Ki Ho Kim, Yeon Hee Seong, Jin Tae Hong
    Abstract:

    The components of Magnolia Officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities. These efficacies have been sold many products as nutritional supplement extracted from bark of Magnolia Officinalis. Thus, to assess and compare neuroprotective effect in the nutritional supplement (Magnolia ExtractTM, Health Freedom Nutrition LLC, USA) and our ethanol extract of Magnolia Officinalis (BioLand LTD, Korea), we investigated memorial improving and anti-Alzheimer’s disease effects of extract products of Magnolia Officinalis in a transgenic AD mice model. Oral pretreatment of two extract products of Magnolia Officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented Aβ accumulation in the brain of Tg2576 mice. In addition, extract products of Magnolia Officinalis also decreased expression of β-site APP cleaving enzyme 1 (BACE1), amyloid precursor protein (APP) and its product, C99. Although both two extract products of Magnolia Officinalis could show preventive effect of memorial dysfunction and Aβ accumulation, our ethanol extract of Magnolia Officinalis (BioLand LTD, Korea) could be more effective than Magnolia ExtractTM (Health Freedom Nutrition LLC, USA). Therefore, our results showed that extract products of Magnolia Officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating β-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

  • protective effect of the ethanol extract of Magnolia Officinalis and 4 o methylhonokiol on scopolamine induced memory impairment and the inhibition of acetylcholinesterase activity
    Journal of Natural Medicines, 2009
    Co-Authors: Yong Kyung Lee, Ki Ho Kim, Dong Yeon Yuk, Tae Il Kim, Young Heui Kim, Kyoung Tae Kim, Beom Jun Lee, Sangyoon Nam, Jin Tae Hong
    Abstract:

    Magnolol, honokiol, and obovatol are well-known bioactive constituents of the bark of Magnolia Officinalis and have been used as traditional Chinese medicines for the treatment of neurosis, anxiety, and stroke. We recently isolated novel active compound (named 4-O-methylhonokiol) from the ethanol extract of Magnolia Officinalis. The present study aimed to test two different doses of ethanol extracts of Magnolia Officinalis (5 and 10 mg/kg/mouse, p.o., 1 week) and 4-O-methylhonokiol (0.75 and 1.5 mg/kg/mouse, p.o., 1 week) administered for 7 days on memory impairment induced by scopolamine (1 mg/kg body weight i.p.) in mice. Memory and learning were evaluated using the Morris water maze and the step-down avoidance test. Both the ethanol extract of Magnolia Officinalis and 4-O-methylhonokiol prevented memory impairment induced by scopolamine in a dose-dependent manner. The ethanol extract of Magnolia Officinalis and 4-O-methylhonokiol also dose-dependently attenuated the scopolamine-induced increase of acetylcholinesterase (AChE) activity in the cortex and hippocampus of mice, and inhibited AChE activity in vitro with IC50 (12 nM). This study, therefore, suggests that the ethanol extract of Magnolia Officinalis and its major ingredient, 4-O-methylhonokiol, may be useful for the prevention of the development or progression of AD.

Hong-bing Yang - One of the best experts on this subject based on the ideXlab platform.

  • The relationship between the quality and the leaf shape of Magnolia Officinalis produced in Hubei enshi
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2012
    Co-Authors: Hong-bing Yang, Lei Shi
    Abstract:

    OBJECTIVE To reveal the relationship between the quality and the leaf shape of Magnolia Officinalis produced in Hubei Enshi. METHODS Determined the content of magnolol and honokiol by HPLC with methanol-water (78:22) and the detective wavelength was 294 nm. RESULTS The content of magnolol and honokiol in the leaf of Magnolia Officinalis was usually higher if the leaf apex was convex and the leaves were short. In certain years, the content of magnolol and honokiol in Cortex Magnoliae Officinalis was positively correlated with the bark thickness. CONCLUSION The leaf shape of Magnolia Officinalis produced in Hubei Enshi is directly related to the phenolic content of leaf itself. But it has nothing to do with the content of magnolol and honokiol in Cortex Magnoliae Officinalis.

  • Construction on ISSR fingerprint of Magnolia Officinalis from Enshi in Hubei province
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2009
    Co-Authors: Hong-bing Yang, Chaolin Liao, Ya-hua Zhan, Guang-hong Cui, Ai-juan Shao, Xian-ming Lin
    Abstract:

    OBJECTIVE To establish the ISSR fingerprint of the cortex of Magnolia Officinalis produced from Enshi. METHODS Cultivar and habit identification was based on Inter-simple sequence repeats (ISSR). RESULTS Two ISSR primes could be used to construct the ISSR fingerprint of M. Officinalis from Enshi. CONCLUSION The ISSR marker is an effective method for the cultivar and habit identification of the cortex of M. officinralis.

  • Phenols in seedling cortex of Magnolia Officinalis from Enshi
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2008
    Co-Authors: Hong-bing Yang, Chaolin Liao, Ya-hua Zhan, Lei Shi, Xin-ming Lin, Xiao-qing Lan
    Abstract:

    OBJECTIVE: To study the phenols in Cortex of Magnolia Officinalis of biernial seedings produced in Enshi, Hubei. METHODS: The content of magnolol and honokiol in cortexes were determined by HPLC. The chromatograms of 10 samples seedling cortexes were recorded and compared. RESULTS: The content of magnolol and honokiol in Cortex of Magnolia Officinalis of the seedlings from Enshi was higher than other samples. There were ten characteristic absorption bands in the HPLC chromatograms, which differed from the cortex of adult trees. CONCLUSION: The results can be used to identify the quality of the seedlings for the breeding.