The Experts below are selected from a list of 27480 Experts worldwide ranked by ideXlab platform
Arash Kheradvar - One of the best experts on this subject based on the ideXlab platform.
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Publisher Correction: Heart beat but not respiration is the Main Driving Force of the systemic venous return in the Fontan circulation.
Scientific Reports, 2019Co-Authors: Dominik Daniel Gabbert, Christopher Hart, Michael Jerosch-herold, Philip Wegner, Inga Voges, Ines Kristo, Abdullah A. L. Bulushi, Jens Scheewe, Mona Salehi Ravesh, Arash KheradvarAbstract:An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Heart beat but not respiration is the Main Driving Force of the systemic venous return in the Fontan circulation
Scientific Reports, 2019Co-Authors: Dominik Daniel Gabbert, Christopher Hart, Michael Jerosch-herold, Philip Wegner, Mona Salehi Ravesh, Inga Voges, Ines Kristo, Abdullah A. L. Bulushi, Jens Scheewe, Arash KheradvarAbstract:The Fontan procedure provides relief from cyanosis in patients with univentricular hearts. A major clinical unmet need is to understand whether the venous flow patterns of the Fontan circulation lead to the development of congestive hepatopathy and other life-threatening complications. Currently, there is no consensus on whether heart beat or respiration is the Main Driving Force of venous return and which one affects the periodic flow changes for the most (i. e., pulsatility). The present study, for the first time, quantified respiratory and cardiac components of the venous flow in the inferior vena cava (IVC) of 14 Fontan patients and 11 normal controls using a novel approach (“physio-matrix”). We found that in contrast to the normal controls, respiration in Fontan patients had a significant effect on venous flow pulsatility, and the ratio of respiration-dependent to the cardiac-dependent pulsatility was positively associated with the retrograde flow. Nevertheless, the Main Driving Force of net IVC flow was the heart beat and not respiration. The separate analysis of the effects of respiration and heart beat provides new insights into the abnormal venous return patterns that may be responsible for adverse effects on liver and bowel of the patients with Fontan circulation.
Nian Wang - One of the best experts on this subject based on the ideXlab platform.
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positive selection is the Main Driving Force for evolution of citrus canker causing xanthomonas
The ISME Journal, 2015Co-Authors: Yunzeng Zhang, Neha Jalan, Erica M Goss, João C Setubal, J B Jones, Xiaoling Deng, Xiaofeng Zhou, Nian WangAbstract:Understanding the evolutionary history and potential of bacterial pathogens is critical to prevent the emergence of new infectious bacterial diseases. Xanthomonas axonopodis subsp. citri (Xac) (synonym X. citri subsp. citri), which causes citrus canker, is one of the hardest-fought plant bacterial pathogens in US history. Here, we sequenced 21 Xac strains (14 XacA, 3 XacA* and 4 XacAw) with different host ranges from North America and Asia and conducted comparative genomic and evolutionary analyses. Our analyses suggest that acquisition of beneficial genes and loss of detrimental genes most likely allowed XacA to infect a broader range of hosts as compared with XacAw and XacA*. Recombination was found to have occurred frequently on the relative ancient branches, but rarely on the young branches of the clonal genealogy. The ratio of recombination/mutation ρ/θ was 0.0790±0.0005, implying that the Xac population was clonal in structure. Positive selection has affected 14% (395 out of 2822) of core genes of the citrus canker-causing Xanthomonas. The genes affected are enriched in ‘carbohydrate transport and metabolism’ and ‘DNA replication, recombination and repair’ genes (P<0.05). Many genes related to virulence, especially genes involved in the type III secretion system and effectors, are affected by positive selection, further highlighting the contribution of positive selection to the evolution of citrus canker-causing Xanthomonas. Our results suggest that both metabolism and virulence genes provide advantages to endow XacA with higher virulence and a wider host range. Our analysis advances our understanding of the genomic basis of specialization by positive selection in bacterial evolution.
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Positive selection is the Main Driving Force for evolution of citrus canker-causing Xanthomonas
The ISME Journal, 2015Co-Authors: Yunzeng Zhang, Neha Jalan, Erica M Goss, João C Setubal, J B Jones, Xiaoling Deng, Xiaofeng Zhou, Nian WangAbstract:Understanding the evolutionary history and potential of bacterial pathogens is critical to prevent the emergence of new infectious bacterial diseases. Xanthomonas axonopodis subsp. citri (Xac) (synonym X. citri subsp. citri), which causes citrus canker, is one of the hardest-fought plant bacterial pathogens in US history. Here, we sequenced 21 Xac strains (14 XacA, 3 XacA* and 4 XacAw) with different host ranges from North America and Asia and conducted comparative genomic and evolutionary analyses. Our analyses suggest that acquisition of beneficial genes and loss of detrimental genes most likely allowed XacA to infect a broader range of hosts as compared with XacAw and XacA*. Recombination was found to have occurred frequently on the relative ancient branches, but rarely on the young branches of the clonal genealogy. The ratio of recombination/mutation ρ/θ was 0.0790±0.0005, implying that the Xac population was clonal in structure. Positive selection has affected 14% (395 out of 2822) of core genes of the citrus canker-causing Xanthomonas. The genes affected are enriched in ‘carbohydrate transport and metabolism’ and ‘DNA replication, recombination and repair’ genes (P
Jeanmarc Zanotti - One of the best experts on this subject based on the ideXlab platform.
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further evidence that interfacial water is the Main Driving Force of protein dynamics a neutron scattering study on perdeuterated c phycocyanin
Physical Chemistry Chemical Physics, 2012Co-Authors: Sophie Combet, Jeanmarc ZanottiAbstract:The fundamental role of hydration water (also called interfacial water) is widely recognized in protein flexibility, especially in the existence of the so-called protein “dynamical transition” at around 220 K. In the present study, we take advantage of perdeuterated C-phycocyanin (CPC) and elastic incoherent neutron scattering (EINS) to distinguish between protein dynamics and interfacial water dynamics. Powders of hydrogenated (hCPC) and perdeuterated (dCPC) CPC protein have been hydrated, respectively, with D2O or H2O and measured by EINS to separately probe protein dynamics (hCPC/D2O) and water dynamics (dCPC/H2O) at different time- and length-scales. We find that “fast” (<20 ps) local mean-square displacements (MSD) of both protein and interfacial water coincide all along the temperature range, with the same dynamical transition temperature at ∼220 K. On higher resolution (<400 ps), two different types of motions can be separated: (i) localized motions with the same amplitude for CPC and hydration water and two transitions at ∼170 and ∼240 K for both; (ii) large scale fluctuations exhibiting for both water molecules and CPC protein a single transition at ∼240 K, with a significantly higher amplitude for the interfacial water than for CPC. Moreover, by comparing these motions with bulk water MSD measured under the same conditions, we show no coupling between bulk water dynamics and protein dynamics all along the temperature range. These results show that interfacial water is the Main “Driving Force” governing both local and large scale motions in proteins.
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Further evidence that interfacial water is the Main “Driving Force” of protein dynamics: a neutron scattering study on perdeuterated C-phycocyanin
Physical Chemistry Chemical Physics, 2012Co-Authors: Sophie Combet, Jeanmarc ZanottiAbstract:The fundamental role of hydration water (also called interfacial water) is widely recognized in protein flexibility, especially in the existence of the so-called protein “dynamical transition” at around 220 K. In the present study, we take advantage of perdeuterated C-phycocyanin (CPC) and elastic incoherent neutron scattering (EINS) to distinguish between protein dynamics and interfacial water dynamics. Powders of hydrogenated (hCPC) and perdeuterated (dCPC) CPC protein have been hydrated, respectively, with D2O or H2O and measured by EINS to separately probe protein dynamics (hCPC/D2O) and water dynamics (dCPC/H2O) at different time- and length-scales. We find that “fast” (
Dominik Daniel Gabbert - One of the best experts on this subject based on the ideXlab platform.
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Publisher Correction: Heart beat but not respiration is the Main Driving Force of the systemic venous return in the Fontan circulation.
Scientific Reports, 2019Co-Authors: Dominik Daniel Gabbert, Christopher Hart, Michael Jerosch-herold, Philip Wegner, Inga Voges, Ines Kristo, Abdullah A. L. Bulushi, Jens Scheewe, Mona Salehi Ravesh, Arash KheradvarAbstract:An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Heart beat but not respiration is the Main Driving Force of the systemic venous return in the Fontan circulation
Scientific Reports, 2019Co-Authors: Dominik Daniel Gabbert, Christopher Hart, Michael Jerosch-herold, Philip Wegner, Mona Salehi Ravesh, Inga Voges, Ines Kristo, Abdullah A. L. Bulushi, Jens Scheewe, Arash KheradvarAbstract:The Fontan procedure provides relief from cyanosis in patients with univentricular hearts. A major clinical unmet need is to understand whether the venous flow patterns of the Fontan circulation lead to the development of congestive hepatopathy and other life-threatening complications. Currently, there is no consensus on whether heart beat or respiration is the Main Driving Force of venous return and which one affects the periodic flow changes for the most (i. e., pulsatility). The present study, for the first time, quantified respiratory and cardiac components of the venous flow in the inferior vena cava (IVC) of 14 Fontan patients and 11 normal controls using a novel approach (“physio-matrix”). We found that in contrast to the normal controls, respiration in Fontan patients had a significant effect on venous flow pulsatility, and the ratio of respiration-dependent to the cardiac-dependent pulsatility was positively associated with the retrograde flow. Nevertheless, the Main Driving Force of net IVC flow was the heart beat and not respiration. The separate analysis of the effects of respiration and heart beat provides new insights into the abnormal venous return patterns that may be responsible for adverse effects on liver and bowel of the patients with Fontan circulation.
Yunzeng Zhang - One of the best experts on this subject based on the ideXlab platform.
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positive selection is the Main Driving Force for evolution of citrus canker causing xanthomonas
The ISME Journal, 2015Co-Authors: Yunzeng Zhang, Neha Jalan, Erica M Goss, João C Setubal, J B Jones, Xiaoling Deng, Xiaofeng Zhou, Nian WangAbstract:Understanding the evolutionary history and potential of bacterial pathogens is critical to prevent the emergence of new infectious bacterial diseases. Xanthomonas axonopodis subsp. citri (Xac) (synonym X. citri subsp. citri), which causes citrus canker, is one of the hardest-fought plant bacterial pathogens in US history. Here, we sequenced 21 Xac strains (14 XacA, 3 XacA* and 4 XacAw) with different host ranges from North America and Asia and conducted comparative genomic and evolutionary analyses. Our analyses suggest that acquisition of beneficial genes and loss of detrimental genes most likely allowed XacA to infect a broader range of hosts as compared with XacAw and XacA*. Recombination was found to have occurred frequently on the relative ancient branches, but rarely on the young branches of the clonal genealogy. The ratio of recombination/mutation ρ/θ was 0.0790±0.0005, implying that the Xac population was clonal in structure. Positive selection has affected 14% (395 out of 2822) of core genes of the citrus canker-causing Xanthomonas. The genes affected are enriched in ‘carbohydrate transport and metabolism’ and ‘DNA replication, recombination and repair’ genes (P<0.05). Many genes related to virulence, especially genes involved in the type III secretion system and effectors, are affected by positive selection, further highlighting the contribution of positive selection to the evolution of citrus canker-causing Xanthomonas. Our results suggest that both metabolism and virulence genes provide advantages to endow XacA with higher virulence and a wider host range. Our analysis advances our understanding of the genomic basis of specialization by positive selection in bacterial evolution.
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Positive selection is the Main Driving Force for evolution of citrus canker-causing Xanthomonas
The ISME Journal, 2015Co-Authors: Yunzeng Zhang, Neha Jalan, Erica M Goss, João C Setubal, J B Jones, Xiaoling Deng, Xiaofeng Zhou, Nian WangAbstract:Understanding the evolutionary history and potential of bacterial pathogens is critical to prevent the emergence of new infectious bacterial diseases. Xanthomonas axonopodis subsp. citri (Xac) (synonym X. citri subsp. citri), which causes citrus canker, is one of the hardest-fought plant bacterial pathogens in US history. Here, we sequenced 21 Xac strains (14 XacA, 3 XacA* and 4 XacAw) with different host ranges from North America and Asia and conducted comparative genomic and evolutionary analyses. Our analyses suggest that acquisition of beneficial genes and loss of detrimental genes most likely allowed XacA to infect a broader range of hosts as compared with XacAw and XacA*. Recombination was found to have occurred frequently on the relative ancient branches, but rarely on the young branches of the clonal genealogy. The ratio of recombination/mutation ρ/θ was 0.0790±0.0005, implying that the Xac population was clonal in structure. Positive selection has affected 14% (395 out of 2822) of core genes of the citrus canker-causing Xanthomonas. The genes affected are enriched in ‘carbohydrate transport and metabolism’ and ‘DNA replication, recombination and repair’ genes (P
