The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
A. Miglio - One of the best experts on this subject based on the ideXlab platform.
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haematological and biochemical reference intervals in adult Maine Coon Cat blood donors
Journal of Feline Medicine and Surgery, 2015Co-Authors: E. Spada, M.t. Antognoni, D. Proverbio, V. Mangili, E. Ferro, A. MiglioAbstract:ObjectivesThe objectives of this study were to derive Maine Coon haematological and biochemical reference intervals (RIs) from adult healthy blood donors, to validate (or reject) the use of published RIs for the general feline population in this breed, and to evaluate the effects of age, sex and weight on the haematological and biochemical results.MethodsHaematological and biochemical data were retrieved retrospectively from a database of 81 healthy adult Maine Coon Cat blood donors and were analysed to generate normal RIs. RIs were determined and compared with established non-breed-specific feline RIs according to the Clinical and Laboratory Standards Institute guidelines and the American Society of Veterinary Clinical Pathology guidelines using Reference Value-Advisor (version 2.1) software.ResultsThe age of the Cats ranged from 1–8 years (mean 4.4 years), 42 were female and 39 were male, and weights ranged from 4.9–8.5 kg (mean 6.7 kg). New Maine Coon RIs were proposed for red blood cell count, mean co...
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Haematological and biochemical reference intervals in adult Maine Coon Cat blood donors
'SAGE Publications', 2015Co-Authors: E. Spada, M.t. Antognoni, D. Proverbio, V. Mangili, E. Ferro, A. MiglioAbstract:Objectives The objectives of this study were to derive Maine Coon haematological and biochemical reference intervals (RIs) from adult healthy blood donors, to validate (or reject) the use of published RIs for the general feline population in this breed, and to evaluate the effects of age, sex and weight on the haematological and biochemical results. Methods Haematological and biochemical data were retrieved retrospectively from a database of 81 healthy adult Maine Coon Cat blood donors and were analysed to generate normal RIs. RIs were determined and compared with established non-breed-specific feline RIs according to the Clinical and Laboratory Standards Institute guidelines and the American Society of Veterinary Clinical Pathology guidelines using Reference Value-Advisor (version 2.1) software. Results The age of the Cats ranged from 1\u20138 years (mean 4.4 years), 42 were female and 39 were male, and weights ranged from 4.9\u20138.5 kg (mean 6.7 kg). New Maine Coon RIs were proposed for red blood cell count, mean corpuscular volume, mean corpuscular haemoglobin concentration, reticulocyte count and percentage. Haematocrit was higher in male Cats (mean HCT 42.9% vs 41% in females; P = 0.001) and in heavier Cats (P = 0.003; slope 1.0, regression equation HCT = 35.1 + 1.0 7 weight). New biochemical RIs were proposed for urea, aspartate aminotransferase, \u3b3-glutamyl transpeptidase (GGT), alkaline phosphatase, total protein and albumin in Maine Coons. Females had higher GGT (median GGT value in females 4.0 vs 3.0 in males; P = 0.011) and albumin values (mean albumin value 3.3 in females vs 3.1 in males; P = 0.013). Conclusions and relevance Currently published RIs for some haematological and biochemical parameters are not appropriate for use in adult Maine Coon Cats. A breed-specific variation could be a plausible explanation for the new haematological and serum biochemical analytes proposed in this study. Breed-specific RIs for Maine Coon Cats will help prevent misinterpretation of laboratory results in diagnosis and in the selection of ideal blood donors
E. Spada - One of the best experts on this subject based on the ideXlab platform.
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haematological and biochemical reference intervals in adult Maine Coon Cat blood donors
Journal of Feline Medicine and Surgery, 2015Co-Authors: E. Spada, M.t. Antognoni, D. Proverbio, V. Mangili, E. Ferro, A. MiglioAbstract:ObjectivesThe objectives of this study were to derive Maine Coon haematological and biochemical reference intervals (RIs) from adult healthy blood donors, to validate (or reject) the use of published RIs for the general feline population in this breed, and to evaluate the effects of age, sex and weight on the haematological and biochemical results.MethodsHaematological and biochemical data were retrieved retrospectively from a database of 81 healthy adult Maine Coon Cat blood donors and were analysed to generate normal RIs. RIs were determined and compared with established non-breed-specific feline RIs according to the Clinical and Laboratory Standards Institute guidelines and the American Society of Veterinary Clinical Pathology guidelines using Reference Value-Advisor (version 2.1) software.ResultsThe age of the Cats ranged from 1–8 years (mean 4.4 years), 42 were female and 39 were male, and weights ranged from 4.9–8.5 kg (mean 6.7 kg). New Maine Coon RIs were proposed for red blood cell count, mean co...
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Haematological and biochemical reference intervals in adult Maine Coon Cat blood donors
'SAGE Publications', 2015Co-Authors: E. Spada, M.t. Antognoni, D. Proverbio, V. Mangili, E. Ferro, A. MiglioAbstract:Objectives The objectives of this study were to derive Maine Coon haematological and biochemical reference intervals (RIs) from adult healthy blood donors, to validate (or reject) the use of published RIs for the general feline population in this breed, and to evaluate the effects of age, sex and weight on the haematological and biochemical results. Methods Haematological and biochemical data were retrieved retrospectively from a database of 81 healthy adult Maine Coon Cat blood donors and were analysed to generate normal RIs. RIs were determined and compared with established non-breed-specific feline RIs according to the Clinical and Laboratory Standards Institute guidelines and the American Society of Veterinary Clinical Pathology guidelines using Reference Value-Advisor (version 2.1) software. Results The age of the Cats ranged from 1\u20138 years (mean 4.4 years), 42 were female and 39 were male, and weights ranged from 4.9\u20138.5 kg (mean 6.7 kg). New Maine Coon RIs were proposed for red blood cell count, mean corpuscular volume, mean corpuscular haemoglobin concentration, reticulocyte count and percentage. Haematocrit was higher in male Cats (mean HCT 42.9% vs 41% in females; P = 0.001) and in heavier Cats (P = 0.003; slope 1.0, regression equation HCT = 35.1 + 1.0 7 weight). New biochemical RIs were proposed for urea, aspartate aminotransferase, \u3b3-glutamyl transpeptidase (GGT), alkaline phosphatase, total protein and albumin in Maine Coons. Females had higher GGT (median GGT value in females 4.0 vs 3.0 in males; P = 0.011) and albumin values (mean albumin value 3.3 in females vs 3.1 in males; P = 0.013). Conclusions and relevance Currently published RIs for some haematological and biochemical parameters are not appropriate for use in adult Maine Coon Cats. A breed-specific variation could be a plausible explanation for the new haematological and serum biochemical analytes proposed in this study. Breed-specific RIs for Maine Coon Cats will help prevent misinterpretation of laboratory results in diagnosis and in the selection of ideal blood donors
Kathryn M. Meurs - One of the best experts on this subject based on the ideXlab platform.
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identifiCation of a novel missense mutation in the fibroblast growth factor 5 gene associated with longhair in the Maine Coon Cat
Human Genetics, 2021Co-Authors: Griffin D Shaffer, Kathryn M. Meurs, Blake C Ballif, Lisa G Shaffer, Helen FloressmithAbstract:Hair length can be a highly variable trait within the Felis Catus species, varying between and within different Cat breeds. Previous research has demonstrated this variability is due to recessive mutations within the fibroblast growth factor 5 (FGF5) gene. Following a genetic screen, four longhaired Maine Coons were identified that had only one copy of a known FGF5 mutation. We performed DNA sequencing on samples from two of these Maine Coons and identified a missense mutation in FGF5 c.577G > A p.Ala193Thr. Genetic screening via restriction digest was then performed on samples from the other two Maine Coons and an additional 273 Cats of various breeds. This screening found that only the two additional Maine Coons were heterozygous for the novel variant. Furthermore, the novel variant was not identified after in silico analysis of 68 whole genome Cat sequences from various breeds, demonstrating that this novel mutation is most likely a breed-specific variant for the Maine Coon, contributing to the longhair phenotype in about 3% of these Cats.
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a cardiac myosin binding protein c mutation in the Maine Coon Cat with familial hypertrophic cardiomyopathy
Human Molecular Genetics, 2005Co-Authors: Kathryn M. Meurs, Marcia J. Munro, Judith A. Kittleson, Jeffrey A. Towbin, Ximena Sanchez, Ryan M David, Neil E Bowles, Peter J Reiser, Keith Dryburgh, Kristin A MacdonaldAbstract:Hypertrophic cardiomyopathy (HCM) is one of the most common causes of sudden cardiac death in young adults and is a familial disease in at least 60% of cases. Causative mutations have been identified in several sarcomeric genes, including the myosin binding protein C (MYBPC3) gene. Although numerous causative mutations have been identified, the pathogenetic process is still poorly understood. A large animal model of familial HCM In the Cat has been identified and may be used for additional study. As the first spontaneous large animal model of this familial disease, feline familial HCM provides a valuable model for investigators to evaluate pathophysiologic processes and therapeutic (pharmacologic or genetic) manipulations. The MYBPC3 gene was chosen as a candidate gene in this model after identifying a reduction in the protein in myocardium from affected Cats in comparison to control Cats (P< 0.001). DNA sequencing was performed and sequence alterations were evaluated for evidence that they changed the amino acid produced, that the amino acid was conserved and that the protein structure was altered. We identified a single base pair change (G to C) in the feline MYBPC3 gene in affected Cats that computationally alters the protein conformation of this gene and results in sarcomeric disorganization. We have identified a causative mutation in the feline MYBPC3 gene that results in the development of familial HCM. This is the first report of a spontaneous mutation causing HCM In a non-human species. It should provide a valuable model for evaluating pathophysiologic processes and therapeutic manipulations.
G D Shelton - One of the best experts on this subject based on the ideXlab platform.
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laminin alpha2 deficiency associated muscular dystrophy in a Maine Coon Cat
Journal of Small Animal Practice, 2003Co-Authors: Luc Poncelet, A Resibois, Eva Engvall, G D SheltonAbstract:A European case of laminin alpha2 deficiency-associated muscular dystrophy in a 12-month-old, female Maine Coon pedigree Cat is reported. The history and eventual clinical presentation of this Cat differed from those of two Cats reported in the USA. In this case, the myopathy was characterised by progressively worsening weakness, muscle atrophy and joint contracture. Tendon reflexes were diminished, and motor nerve conduction velocities were slowed. Muscle biopsy demonstrated a dystrophic phenotype with endomysial fibrosis. Occasional thinly myelinated nerve fibres were present within a peripheral nerve specimen. Poorly myelinated fibres were also found at the root level on necropsy specimens. Immunohistochemical staining revealed the absence of laminin alpha2. The Cat's family history did not indiCate genetic transmission of the disease.
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laminin α2 deficiency associated muscular dystrophy in a Maine Coon Cat
Journal of Small Animal Practice, 2003Co-Authors: Luc Poncelet, A Resibois, Eva Engvall, G D SheltonAbstract:A European case of laminin alpha2 deficiency-associated muscular dystrophy in a 12-month-old, female Maine Coon pedigree Cat is reported. The history and eventual clinical presentation of this Cat differed from those of two Cats reported in the USA. In this case, the myopathy was characterised by progressively worsening weakness, muscle atrophy and joint contracture. Tendon reflexes were diminished, and motor nerve conduction velocities were slowed. Muscle biopsy demonstrated a dystrophic phenotype with endomysial fibrosis. Occasional thinly myelinated nerve fibres were present within a peripheral nerve specimen. Poorly myelinated fibres were also found at the root level on necropsy specimens. Immunohistochemical staining revealed the absence of laminin alpha2. The Cat's family history did not indiCate genetic transmission of the disease.
D. Proverbio - One of the best experts on this subject based on the ideXlab platform.
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haematological and biochemical reference intervals in adult Maine Coon Cat blood donors
Journal of Feline Medicine and Surgery, 2015Co-Authors: E. Spada, M.t. Antognoni, D. Proverbio, V. Mangili, E. Ferro, A. MiglioAbstract:ObjectivesThe objectives of this study were to derive Maine Coon haematological and biochemical reference intervals (RIs) from adult healthy blood donors, to validate (or reject) the use of published RIs for the general feline population in this breed, and to evaluate the effects of age, sex and weight on the haematological and biochemical results.MethodsHaematological and biochemical data were retrieved retrospectively from a database of 81 healthy adult Maine Coon Cat blood donors and were analysed to generate normal RIs. RIs were determined and compared with established non-breed-specific feline RIs according to the Clinical and Laboratory Standards Institute guidelines and the American Society of Veterinary Clinical Pathology guidelines using Reference Value-Advisor (version 2.1) software.ResultsThe age of the Cats ranged from 1–8 years (mean 4.4 years), 42 were female and 39 were male, and weights ranged from 4.9–8.5 kg (mean 6.7 kg). New Maine Coon RIs were proposed for red blood cell count, mean co...
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Haematological and biochemical reference intervals in adult Maine Coon Cat blood donors
'SAGE Publications', 2015Co-Authors: E. Spada, M.t. Antognoni, D. Proverbio, V. Mangili, E. Ferro, A. MiglioAbstract:Objectives The objectives of this study were to derive Maine Coon haematological and biochemical reference intervals (RIs) from adult healthy blood donors, to validate (or reject) the use of published RIs for the general feline population in this breed, and to evaluate the effects of age, sex and weight on the haematological and biochemical results. Methods Haematological and biochemical data were retrieved retrospectively from a database of 81 healthy adult Maine Coon Cat blood donors and were analysed to generate normal RIs. RIs were determined and compared with established non-breed-specific feline RIs according to the Clinical and Laboratory Standards Institute guidelines and the American Society of Veterinary Clinical Pathology guidelines using Reference Value-Advisor (version 2.1) software. Results The age of the Cats ranged from 1\u20138 years (mean 4.4 years), 42 were female and 39 were male, and weights ranged from 4.9\u20138.5 kg (mean 6.7 kg). New Maine Coon RIs were proposed for red blood cell count, mean corpuscular volume, mean corpuscular haemoglobin concentration, reticulocyte count and percentage. Haematocrit was higher in male Cats (mean HCT 42.9% vs 41% in females; P = 0.001) and in heavier Cats (P = 0.003; slope 1.0, regression equation HCT = 35.1 + 1.0 7 weight). New biochemical RIs were proposed for urea, aspartate aminotransferase, \u3b3-glutamyl transpeptidase (GGT), alkaline phosphatase, total protein and albumin in Maine Coons. Females had higher GGT (median GGT value in females 4.0 vs 3.0 in males; P = 0.011) and albumin values (mean albumin value 3.3 in females vs 3.1 in males; P = 0.013). Conclusions and relevance Currently published RIs for some haematological and biochemical parameters are not appropriate for use in adult Maine Coon Cats. A breed-specific variation could be a plausible explanation for the new haematological and serum biochemical analytes proposed in this study. Breed-specific RIs for Maine Coon Cats will help prevent misinterpretation of laboratory results in diagnosis and in the selection of ideal blood donors
