The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Ziwei Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates.
    Journal of Medical Microbiology, 2020
    Co-Authors: Ziwei Zhang, Ni Liang, Yun Luo, Yi-ming Fan
    Abstract:

    Introduction. Malassezia Folliculitis (MF) and pityriasis versicolor (PV) are common dermatoses caused by Malassezia species. Their molecular epidemiology, drug susceptibility and exoenzymes are rarely reported in China. Aim. To investigate the molecular epidemiology, drug susceptibility and enzymatic profile of Malassezia clinical isolates. Methodology. Malassezia strains were recovered from MF and PV patients and healthy subjects (HS) and identified by sequencing analysis. The minimum inhibitory concentrations (MICs) of nine antifungals (posaconazole, voriconazole, itraconazole, fluconazole, ketoconazole, miconazole, bifonazole, terbinafine and caspofungin) and tacrolimus, the interactions between three antifungals (itraconazole, ketoconazole and terbinafine) and tacrolimus, and the extracellular enzyme profile were evaluated using broth and checkerboard microdilution and the Api-Zym system, respectively. Results. Among 392 Malassezia isolates from 729 subjects (289 MF, 218 PV and 222 HS), Malassezia furfur and Malassezia globosa accounted for 67.86 and 18.88 %, respectively. M. furfur was the major species in MF and PV patients and HS. Among 60M. furfur and 50M. globosa strains, the MICs for itraconazole, posaconazole, voriconazole and ketoconazole were

  • overexpression of nlrp3 nlrc4 and aim2 inflammasomes and their priming associated molecules tlr2 tlr4 dectin 1 dectin 2 and nfκb in Malassezia Folliculitis
    Mycoses, 2018
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

  • Overexpression of NLRP3, NLRC4 and AIM2 inflammasomes and their priming‐associated molecules (TLR2, TLR4, Dectin‐1, Dectin‐2 and NFκB) in Malassezia Folliculitis
    Mycoses, 2017
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

Ni Liang - One of the best experts on this subject based on the ideXlab platform.

  • Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates.
    Journal of Medical Microbiology, 2020
    Co-Authors: Ziwei Zhang, Ni Liang, Yun Luo, Yi-ming Fan
    Abstract:

    Introduction. Malassezia Folliculitis (MF) and pityriasis versicolor (PV) are common dermatoses caused by Malassezia species. Their molecular epidemiology, drug susceptibility and exoenzymes are rarely reported in China. Aim. To investigate the molecular epidemiology, drug susceptibility and enzymatic profile of Malassezia clinical isolates. Methodology. Malassezia strains were recovered from MF and PV patients and healthy subjects (HS) and identified by sequencing analysis. The minimum inhibitory concentrations (MICs) of nine antifungals (posaconazole, voriconazole, itraconazole, fluconazole, ketoconazole, miconazole, bifonazole, terbinafine and caspofungin) and tacrolimus, the interactions between three antifungals (itraconazole, ketoconazole and terbinafine) and tacrolimus, and the extracellular enzyme profile were evaluated using broth and checkerboard microdilution and the Api-Zym system, respectively. Results. Among 392 Malassezia isolates from 729 subjects (289 MF, 218 PV and 222 HS), Malassezia furfur and Malassezia globosa accounted for 67.86 and 18.88 %, respectively. M. furfur was the major species in MF and PV patients and HS. Among 60M. furfur and 50M. globosa strains, the MICs for itraconazole, posaconazole, voriconazole and ketoconazole were

  • overexpression of nlrp3 nlrc4 and aim2 inflammasomes and their priming associated molecules tlr2 tlr4 dectin 1 dectin 2 and nfκb in Malassezia Folliculitis
    Mycoses, 2018
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

  • Overexpression of NLRP3, NLRC4 and AIM2 inflammasomes and their priming‐associated molecules (TLR2, TLR4, Dectin‐1, Dectin‐2 and NFκB) in Malassezia Folliculitis
    Mycoses, 2017
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

Yayun Wu - One of the best experts on this subject based on the ideXlab platform.

  • overexpression of nlrp3 nlrc4 and aim2 inflammasomes and their priming associated molecules tlr2 tlr4 dectin 1 dectin 2 and nfκb in Malassezia Folliculitis
    Mycoses, 2018
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

  • Overexpression of NLRP3, NLRC4 and AIM2 inflammasomes and their priming‐associated molecules (TLR2, TLR4, Dectin‐1, Dectin‐2 and NFκB) in Malassezia Folliculitis
    Mycoses, 2017
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

Yanping Yang - One of the best experts on this subject based on the ideXlab platform.

  • overexpression of nlrp3 nlrc4 and aim2 inflammasomes and their priming associated molecules tlr2 tlr4 dectin 1 dectin 2 and nfκb in Malassezia Folliculitis
    Mycoses, 2018
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

  • Overexpression of NLRP3, NLRC4 and AIM2 inflammasomes and their priming‐associated molecules (TLR2, TLR4, Dectin‐1, Dectin‐2 and NFκB) in Malassezia Folliculitis
    Mycoses, 2017
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

Wei Li - One of the best experts on this subject based on the ideXlab platform.

  • overexpression of nlrp3 nlrc4 and aim2 inflammasomes and their priming associated molecules tlr2 tlr4 dectin 1 dectin 2 and nfκb in Malassezia Folliculitis
    Mycoses, 2018
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.

  • Overexpression of NLRP3, NLRC4 and AIM2 inflammasomes and their priming‐associated molecules (TLR2, TLR4, Dectin‐1, Dectin‐2 and NFκB) in Malassezia Folliculitis
    Mycoses, 2017
    Co-Authors: Ni Liang, Yayun Wu, Yanping Yang, Wei Li, Ziwei Zhang
    Abstract:

    Background The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia Folliculitis (MF) remains unclear. Objectives To determine expression of four canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Methods Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1, and IL-1β mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Results Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1β, TLR2, TLR4, Dectin-1, Dectin-2, and NFκB expression was upregulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P 0.05). qRT-PCR showed that levels of NLRP3, Casp-1, and IL-1β mRNA were significantly increased (P 0.05). Conclusions Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF. This article is protected by copyright. All rights reserved.