The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform
Eevariitta Savolainen - One of the best experts on this subject based on the ideXlab platform.
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the effect of Malotilate on type iii and type iv collagen laminin and fibronectin metabolism in dimethylnitrosamine induced liver fibrosis in the rat
Journal of Hepatology, 1996Co-Authors: Lasse Ryhanen, Frej Stenbäck, Leena Alakokko, Eevariitta SavolainenAbstract:Abstract Background/Aims: Dimethylnitrosamine-induced liver damage was used as an experimental model to study the effect of Malotilate on liver fibrosis. Methods: Deposition of type III and IV collagens, laminin and fibronectin were studied from liver section by immunohistochemical techniques using specific antibodies. Serum concentrations of aminoterminal propeptide of type III procollagen, and aminoterminal and carboxyterminal domains of type IV collagen were determined by radioimmunoassays from both Malotilate-treated and untreated animals with dimethylnitrosamine injury. Results: A significant elevation of all three serum parameters was observed after 3 weeks of hepatic injury in animals without Malotilate treatment, and a constant increase was noted in the amounts of hepatic type III and IV collagens, laminin and fibronectin. Malotilate prevented increases in serum markers of type III and IV collagen synthesis as well as accumulation of the collagens, laminin and fibronectin in the liver. Conclusions: The results suggest that serum marker determinations can be used to monitor changes in type III and IV collagen synthesis in the liver. The data indicate that Malotilate has a preventive effect in dimethylnitrosamine-induced experimental hepatic fibrosis.
Lasse Ryhanen - One of the best experts on this subject based on the ideXlab platform.
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the effect of Malotilate on type iii and type iv collagen laminin and fibronectin metabolism in dimethylnitrosamine induced liver fibrosis in the rat
Journal of Hepatology, 1996Co-Authors: Lasse Ryhanen, Frej Stenbäck, Leena Alakokko, Eevariitta SavolainenAbstract:Abstract Background/Aims: Dimethylnitrosamine-induced liver damage was used as an experimental model to study the effect of Malotilate on liver fibrosis. Methods: Deposition of type III and IV collagens, laminin and fibronectin were studied from liver section by immunohistochemical techniques using specific antibodies. Serum concentrations of aminoterminal propeptide of type III procollagen, and aminoterminal and carboxyterminal domains of type IV collagen were determined by radioimmunoassays from both Malotilate-treated and untreated animals with dimethylnitrosamine injury. Results: A significant elevation of all three serum parameters was observed after 3 weeks of hepatic injury in animals without Malotilate treatment, and a constant increase was noted in the amounts of hepatic type III and IV collagens, laminin and fibronectin. Malotilate prevented increases in serum markers of type III and IV collagen synthesis as well as accumulation of the collagens, laminin and fibronectin in the liver. Conclusions: The results suggest that serum marker determinations can be used to monitor changes in type III and IV collagen synthesis in the liver. The data indicate that Malotilate has a preventive effect in dimethylnitrosamine-induced experimental hepatic fibrosis.
Frej Stenbäck - One of the best experts on this subject based on the ideXlab platform.
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the effect of Malotilate on type iii and type iv collagen laminin and fibronectin metabolism in dimethylnitrosamine induced liver fibrosis in the rat
Journal of Hepatology, 1996Co-Authors: Lasse Ryhanen, Frej Stenbäck, Leena Alakokko, Eevariitta SavolainenAbstract:Abstract Background/Aims: Dimethylnitrosamine-induced liver damage was used as an experimental model to study the effect of Malotilate on liver fibrosis. Methods: Deposition of type III and IV collagens, laminin and fibronectin were studied from liver section by immunohistochemical techniques using specific antibodies. Serum concentrations of aminoterminal propeptide of type III procollagen, and aminoterminal and carboxyterminal domains of type IV collagen were determined by radioimmunoassays from both Malotilate-treated and untreated animals with dimethylnitrosamine injury. Results: A significant elevation of all three serum parameters was observed after 3 weeks of hepatic injury in animals without Malotilate treatment, and a constant increase was noted in the amounts of hepatic type III and IV collagens, laminin and fibronectin. Malotilate prevented increases in serum markers of type III and IV collagen synthesis as well as accumulation of the collagens, laminin and fibronectin in the liver. Conclusions: The results suggest that serum marker determinations can be used to monitor changes in type III and IV collagen synthesis in the liver. The data indicate that Malotilate has a preventive effect in dimethylnitrosamine-induced experimental hepatic fibrosis.
Leena Alakokko - One of the best experts on this subject based on the ideXlab platform.
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the effect of Malotilate on type iii and type iv collagen laminin and fibronectin metabolism in dimethylnitrosamine induced liver fibrosis in the rat
Journal of Hepatology, 1996Co-Authors: Lasse Ryhanen, Frej Stenbäck, Leena Alakokko, Eevariitta SavolainenAbstract:Abstract Background/Aims: Dimethylnitrosamine-induced liver damage was used as an experimental model to study the effect of Malotilate on liver fibrosis. Methods: Deposition of type III and IV collagens, laminin and fibronectin were studied from liver section by immunohistochemical techniques using specific antibodies. Serum concentrations of aminoterminal propeptide of type III procollagen, and aminoterminal and carboxyterminal domains of type IV collagen were determined by radioimmunoassays from both Malotilate-treated and untreated animals with dimethylnitrosamine injury. Results: A significant elevation of all three serum parameters was observed after 3 weeks of hepatic injury in animals without Malotilate treatment, and a constant increase was noted in the amounts of hepatic type III and IV collagens, laminin and fibronectin. Malotilate prevented increases in serum markers of type III and IV collagen synthesis as well as accumulation of the collagens, laminin and fibronectin in the liver. Conclusions: The results suggest that serum marker determinations can be used to monitor changes in type III and IV collagen synthesis in the liver. The data indicate that Malotilate has a preventive effect in dimethylnitrosamine-induced experimental hepatic fibrosis.
Jan Mojzis - One of the best experts on this subject based on the ideXlab platform.
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effect of Malotilate on ethanol induced gastric mucosal damage in capsaicin pretreated rats
Physiological Research, 1999Co-Authors: L Mirossay, A Kohut, Jan MojzisAbstract:We studied the role of afferent sensory neurons in Malotilate-mediated gastric mucosal protection. Intact and capsaicin sensory-denervated rats were used in the experiments. Gross gastric mucosal injury was assessed and evaluated as a main criterion of the gastroprotective effect of the tested substances. Besides Malotilate, methyl-prostaglandin E2 was applied alone or in combination with Malotilate to compare the effects and the mechanism of action of both substances. The results revealed that both Malotilate as well as methyl-prostaglandin E2 exerted a significant protective action on 96% ethanol-induced gastric mucosal damage. However, there were no significant differences between intact and capsaicin-denervated rats. Only the use of 50% ethanol as a milder mucosal irritating agent resulted in significant differences in both groups of animals. We propose that Malotilate (like methyl-prostaglandin E2) has a gastroprotective effect on ethanol-induced gastric mucosal injury. This effect is partly dependent on the sensory nervous system and the combination of both above substances has an additive effect.
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gastroprotective effect of Malotilate in indomethacin and ethanol induced gastric mucosal damage
Physiological Research, 1996Co-Authors: L Mirossay, Jan Mojzis, Z Sallingova, J Bodnar, M Benický, A Boor, A KohutAbstract:Malotilate as a synthetic substance shares comparable hepatoprotective properties with various flavonoids. The gastroprotective effect of some flavonoids prompted us to ascertain the similar effectiveness of Malotilate. The possible gastroprotectivity was examined in gastric mucosal damage in rats induced by indomethacin (20 mg.kg-1) or ethanol (96%). Oral pretreatment with Malotilate (25, 50, 100, 200 and 400 mg.kg-1) reduced the extent of lesions induced by both indomethacin and ethanol. Histological analyses also revealed a mitigating effect on the severity of gastric mucosal lesions. Similar results were obtained in the group of rats pretreated with 5 mg.kg-1 indomethacin followed by oral administration of 96% ethanol. This finding suggests that the effect of Malotilate on rat gastric mucosa is independent of endogenous prostaglandin production.