The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Rozina S Ali - One of the best experts on this subject based on the ideXlab platform.
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expression of the peptide antibiotics human β defensin 1 and human β defensin 2 in normal human skin
Journal of Investigative Dermatology, 2001Co-Authors: Amy Falconer, Mohammed Ikram, Clare E Bissett, R Cerio, Anthony G Quinn, Rozina S AliAbstract:Normal human skin is remarkably resistant to infection from the large numbers of microorganisms that routinely colonize its surface. In addition to the role of skin as a mechanical barrier, it has long been recognized that skin and other epithelia can produce a range of anti-microbial chemicals that play an important part in eliminating potential cutaneous pathogens. Anti-microbial peptides are an important evolutionarily conserved innate host defense mechanism in many organisms. Human β defensin-1 and -2 are cysteine-rich, cationic, low molecular weight anti-microbial peptides that have recently been shown to be expressed in epithelial tissues. In this study, we describe the characterization of human β defensin-1 and -2 mRNA and peptide expression in normal human skin. Using reverse transcription–polymerase chain reaction we demonstrate that human β defensin-1 is consistently expressed in skin samples from various body sites. Human β defensin-2 demonstrates expression that is more variable and is more readily detectable in facial skin and foreskin compared with skin from abdomen and breast. In situ hybridization localizes the human β defensin-1 and -2 transcripts to keratinocytes within interfollicular skin. Using specific antibodies, we have shown that human β defensin-1 and -2 peptides are localized to the Malpighian Layer of the epidermis and/or stratum corneum and that there are interindividual and site-specific differences in intensity of immunostaining and the pattern of peptide localization. The localization of human β defensins to the outer Layer of the skin is consistent with the hypothesis that human β defensins play an essential part in cutaneous innate immunity.
Amy Falconer - One of the best experts on this subject based on the ideXlab platform.
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expression of the peptide antibiotics human β defensin 1 and human β defensin 2 in normal human skin
Journal of Investigative Dermatology, 2001Co-Authors: Amy Falconer, Mohammed Ikram, Clare E Bissett, R Cerio, Anthony G Quinn, Rozina S AliAbstract:Normal human skin is remarkably resistant to infection from the large numbers of microorganisms that routinely colonize its surface. In addition to the role of skin as a mechanical barrier, it has long been recognized that skin and other epithelia can produce a range of anti-microbial chemicals that play an important part in eliminating potential cutaneous pathogens. Anti-microbial peptides are an important evolutionarily conserved innate host defense mechanism in many organisms. Human β defensin-1 and -2 are cysteine-rich, cationic, low molecular weight anti-microbial peptides that have recently been shown to be expressed in epithelial tissues. In this study, we describe the characterization of human β defensin-1 and -2 mRNA and peptide expression in normal human skin. Using reverse transcription–polymerase chain reaction we demonstrate that human β defensin-1 is consistently expressed in skin samples from various body sites. Human β defensin-2 demonstrates expression that is more variable and is more readily detectable in facial skin and foreskin compared with skin from abdomen and breast. In situ hybridization localizes the human β defensin-1 and -2 transcripts to keratinocytes within interfollicular skin. Using specific antibodies, we have shown that human β defensin-1 and -2 peptides are localized to the Malpighian Layer of the epidermis and/or stratum corneum and that there are interindividual and site-specific differences in intensity of immunostaining and the pattern of peptide localization. The localization of human β defensins to the outer Layer of the skin is consistent with the hypothesis that human β defensins play an essential part in cutaneous innate immunity.
Anthony G Quinn - One of the best experts on this subject based on the ideXlab platform.
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expression of the peptide antibiotics human β defensin 1 and human β defensin 2 in normal human skin
Journal of Investigative Dermatology, 2001Co-Authors: Amy Falconer, Mohammed Ikram, Clare E Bissett, R Cerio, Anthony G Quinn, Rozina S AliAbstract:Normal human skin is remarkably resistant to infection from the large numbers of microorganisms that routinely colonize its surface. In addition to the role of skin as a mechanical barrier, it has long been recognized that skin and other epithelia can produce a range of anti-microbial chemicals that play an important part in eliminating potential cutaneous pathogens. Anti-microbial peptides are an important evolutionarily conserved innate host defense mechanism in many organisms. Human β defensin-1 and -2 are cysteine-rich, cationic, low molecular weight anti-microbial peptides that have recently been shown to be expressed in epithelial tissues. In this study, we describe the characterization of human β defensin-1 and -2 mRNA and peptide expression in normal human skin. Using reverse transcription–polymerase chain reaction we demonstrate that human β defensin-1 is consistently expressed in skin samples from various body sites. Human β defensin-2 demonstrates expression that is more variable and is more readily detectable in facial skin and foreskin compared with skin from abdomen and breast. In situ hybridization localizes the human β defensin-1 and -2 transcripts to keratinocytes within interfollicular skin. Using specific antibodies, we have shown that human β defensin-1 and -2 peptides are localized to the Malpighian Layer of the epidermis and/or stratum corneum and that there are interindividual and site-specific differences in intensity of immunostaining and the pattern of peptide localization. The localization of human β defensins to the outer Layer of the skin is consistent with the hypothesis that human β defensins play an essential part in cutaneous innate immunity.
R Cerio - One of the best experts on this subject based on the ideXlab platform.
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expression of the peptide antibiotics human β defensin 1 and human β defensin 2 in normal human skin
Journal of Investigative Dermatology, 2001Co-Authors: Amy Falconer, Mohammed Ikram, Clare E Bissett, R Cerio, Anthony G Quinn, Rozina S AliAbstract:Normal human skin is remarkably resistant to infection from the large numbers of microorganisms that routinely colonize its surface. In addition to the role of skin as a mechanical barrier, it has long been recognized that skin and other epithelia can produce a range of anti-microbial chemicals that play an important part in eliminating potential cutaneous pathogens. Anti-microbial peptides are an important evolutionarily conserved innate host defense mechanism in many organisms. Human β defensin-1 and -2 are cysteine-rich, cationic, low molecular weight anti-microbial peptides that have recently been shown to be expressed in epithelial tissues. In this study, we describe the characterization of human β defensin-1 and -2 mRNA and peptide expression in normal human skin. Using reverse transcription–polymerase chain reaction we demonstrate that human β defensin-1 is consistently expressed in skin samples from various body sites. Human β defensin-2 demonstrates expression that is more variable and is more readily detectable in facial skin and foreskin compared with skin from abdomen and breast. In situ hybridization localizes the human β defensin-1 and -2 transcripts to keratinocytes within interfollicular skin. Using specific antibodies, we have shown that human β defensin-1 and -2 peptides are localized to the Malpighian Layer of the epidermis and/or stratum corneum and that there are interindividual and site-specific differences in intensity of immunostaining and the pattern of peptide localization. The localization of human β defensins to the outer Layer of the skin is consistent with the hypothesis that human β defensins play an essential part in cutaneous innate immunity.
Mohammed Ikram - One of the best experts on this subject based on the ideXlab platform.
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expression of the peptide antibiotics human β defensin 1 and human β defensin 2 in normal human skin
Journal of Investigative Dermatology, 2001Co-Authors: Amy Falconer, Mohammed Ikram, Clare E Bissett, R Cerio, Anthony G Quinn, Rozina S AliAbstract:Normal human skin is remarkably resistant to infection from the large numbers of microorganisms that routinely colonize its surface. In addition to the role of skin as a mechanical barrier, it has long been recognized that skin and other epithelia can produce a range of anti-microbial chemicals that play an important part in eliminating potential cutaneous pathogens. Anti-microbial peptides are an important evolutionarily conserved innate host defense mechanism in many organisms. Human β defensin-1 and -2 are cysteine-rich, cationic, low molecular weight anti-microbial peptides that have recently been shown to be expressed in epithelial tissues. In this study, we describe the characterization of human β defensin-1 and -2 mRNA and peptide expression in normal human skin. Using reverse transcription–polymerase chain reaction we demonstrate that human β defensin-1 is consistently expressed in skin samples from various body sites. Human β defensin-2 demonstrates expression that is more variable and is more readily detectable in facial skin and foreskin compared with skin from abdomen and breast. In situ hybridization localizes the human β defensin-1 and -2 transcripts to keratinocytes within interfollicular skin. Using specific antibodies, we have shown that human β defensin-1 and -2 peptides are localized to the Malpighian Layer of the epidermis and/or stratum corneum and that there are interindividual and site-specific differences in intensity of immunostaining and the pattern of peptide localization. The localization of human β defensins to the outer Layer of the skin is consistent with the hypothesis that human β defensins play an essential part in cutaneous innate immunity.