The Experts below are selected from a list of 17370 Experts worldwide ranked by ideXlab platform
Xiaojun Zhang - One of the best experts on this subject based on the ideXlab platform.
-
immunoaffinity chromatography purification and ultrahigh performance liquid chromatography tandem mass spectrometry determination of tetrodotoxin in Marine Organisms
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Xiaojun Zhang, Ying Wang, Tao Jiang, Jian WangAbstract:A highly selective and sensitive method was developed for the determination of tetrodotoxin (TTX) in Marine Organisms by immunoaffinity chromatography (IAC) purification coupled with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). An IAC column was prepared and used to cleanup the extracted samples. The operating conditions of the IAC column were optimized, and the capacity of new IAC column was found to be 1106 ng mL–1, which was sufficient for TTX determination. The MS/MS conditions and UPLC mobile phase were also studied to optimize the operation conditions. Fortified Marine Organism samples at levels of 0.3–5.0 ng g–1 were utilized, and the average recoveries were 86.5–103.6% with intra- and inter-day relative standard deviations less than 7.22 and 9.88%, respectively. The limits of detection and quantification were 0.1 and 0.3 ng g–1, respectively. The method was later successfully applied for the determination of TTX in 100 Marine Organism samples collected from lo...
-
immunoaffinity chromatography purification and ultrahigh performance liquid chromatography tandem mass spectrometry determination of tetrodotoxin in Marine Organisms
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Xiaojun Zhang, Ying Wang, Tao Jiang, Zhongyong Yan, Jing Wang, Xiumei Sun, Yuanming GuoAbstract:A highly selective and sensitive method was developed for the determination of tetrodotoxin (TTX) in Marine Organisms by immunoaffinity chromatography (IAC) purification coupled with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). An IAC column was prepared and used to cleanup the extracted samples. The operating conditions of the IAC column were optimized, and the capacity of new IAC column was found to be 1106 ng mL(-1), which was sufficient for TTX determination. The MS/MS conditions and UPLC mobile phase were also studied to optimize the operation conditions. Fortified Marine Organism samples at levels of 0.3-5.0 ng g(-1) were utilized, and the average recoveries were 86.5-103.6% with intra- and inter-day relative standard deviations less than 7.22 and 9.88%, respectively. The limits of detection and quantification were 0.1 and 0.3 ng g(-1), respectively. The method was later successfully applied for the determination of TTX in 100 Marine Organism samples collected from local markets.
Jian Wang - One of the best experts on this subject based on the ideXlab platform.
-
immunoaffinity chromatography purification and ultrahigh performance liquid chromatography tandem mass spectrometry determination of tetrodotoxin in Marine Organisms
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Xiaojun Zhang, Ying Wang, Tao Jiang, Jian WangAbstract:A highly selective and sensitive method was developed for the determination of tetrodotoxin (TTX) in Marine Organisms by immunoaffinity chromatography (IAC) purification coupled with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). An IAC column was prepared and used to cleanup the extracted samples. The operating conditions of the IAC column were optimized, and the capacity of new IAC column was found to be 1106 ng mL–1, which was sufficient for TTX determination. The MS/MS conditions and UPLC mobile phase were also studied to optimize the operation conditions. Fortified Marine Organism samples at levels of 0.3–5.0 ng g–1 were utilized, and the average recoveries were 86.5–103.6% with intra- and inter-day relative standard deviations less than 7.22 and 9.88%, respectively. The limits of detection and quantification were 0.1 and 0.3 ng g–1, respectively. The method was later successfully applied for the determination of TTX in 100 Marine Organism samples collected from lo...
Yuanming Guo - One of the best experts on this subject based on the ideXlab platform.
-
immunoaffinity chromatography purification and ultrahigh performance liquid chromatography tandem mass spectrometry determination of tetrodotoxin in Marine Organisms
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Xiaojun Zhang, Ying Wang, Tao Jiang, Zhongyong Yan, Jing Wang, Xiumei Sun, Yuanming GuoAbstract:A highly selective and sensitive method was developed for the determination of tetrodotoxin (TTX) in Marine Organisms by immunoaffinity chromatography (IAC) purification coupled with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). An IAC column was prepared and used to cleanup the extracted samples. The operating conditions of the IAC column were optimized, and the capacity of new IAC column was found to be 1106 ng mL(-1), which was sufficient for TTX determination. The MS/MS conditions and UPLC mobile phase were also studied to optimize the operation conditions. Fortified Marine Organism samples at levels of 0.3-5.0 ng g(-1) were utilized, and the average recoveries were 86.5-103.6% with intra- and inter-day relative standard deviations less than 7.22 and 9.88%, respectively. The limits of detection and quantification were 0.1 and 0.3 ng g(-1), respectively. The method was later successfully applied for the determination of TTX in 100 Marine Organism samples collected from local markets.
Thanos Dailianis - One of the best experts on this subject based on the ideXlab platform.
-
a de novo transcriptome assembly for the bath sponge spongia officinalis adjusting for microsymbionts
BMC Research Notes, 2019Co-Authors: Tereza Manousaki, Vasiliki Koutsouveli, Jacques Lagnel, Spyridon Kollias, Costas S Tsigenopoulos, Christos Arvanitidis, Antonios Magoulas, C Dounas, Thanos DailianisAbstract:We report a transcriptome acquisition for the bath sponge Spongia officinalis, a non-model Marine Organism that hosts rich symbiotic microbial communities. To this end, a pipeline was developed to efficiently separate between bacterial expressed genes from those of eukaryotic origin. The transcriptome was produced to support the assessment of gene expression and, thus, the response of the sponge, to elevated temperatures, replicating conditions currently occurring in its native habitat. We describe the assembled transcriptome along with the bioinformatic pipeline used to discriminate between signals of metazoan and prokaryotic origin. The pipeline involves standard read pre-processing steps and incorporates extra analyses to identify and filter prokaryotic reads out of the analysis. The proposed pipeline can be followed to overcome the technical RNASeq problems characteristic for symbiont-rich metazoan Organisms with low or non-existent tissue differentiation, such as sponges and cnidarians. At the same time, it can be valuable towards the development of approaches for parallel transcriptomic studies of symbiotic communities and the host.
-
a de novo transcriptome assembly for the bath sponge spongia officinalis adjusting for microsymbionts
BMC Research Notes, 2019Co-Authors: Tereza Manousaki, Vasiliki Koutsouveli, Jacques Lagnel, Spyridon Kollias, Costas S Tsigenopoulos, Christos Arvanitidis, Antonios Magoulas, C Dounas, Thanos DailianisAbstract:OBJECTIVES We report a transcriptome acquisition for the bath sponge Spongia officinalis, a non-model Marine Organism that hosts rich symbiotic microbial communities. To this end, a pipeline was developed to efficiently separate between bacterial expressed genes from those of eukaryotic origin. The transcriptome was produced to support the assessment of gene expression and, thus, the response of the sponge, to elevated temperatures, replicating conditions currently occurring in its native habitat. DATA DESCRIPTION We describe the assembled transcriptome along with the bioinformatic pipeline used to discriminate between signals of metazoan and prokaryotic origin. The pipeline involves standard read pre-processing steps and incorporates extra analyses to identify and filter prokaryotic reads out of the analysis. The proposed pipeline can be followed to overcome the technical RNASeq problems characteristic for symbiont-rich metazoan Organisms with low or non-existent tissue differentiation, such as sponges and cnidarians. At the same time, it can be valuable towards the development of approaches for parallel transcriptomic studies of symbiotic communities and the host.
Joan O Grimalt - One of the best experts on this subject based on the ideXlab platform.
-
long chain n alkyl diols hydroxy ketones and sterols in a Marine eustigmatophyte nannochloropsis gaditana and in brachionus plicatilis feeding on the algae
Organic Geochemistry, 2003Co-Authors: Laurence Mejanelle, Angels Sanchezgargallo, Ilhem Bentaleb, Joan O GrimaltAbstract:Base-hydrolyzed sterols and long-chain alcohols of a Marine eustigmatophyte, Nannochloropsis gaditana have been studied. Sterol composition of N. gaditana is similar to that of other member of this genus, with predominance of cholest-5-en-3β-ol and 24-ethylcholest-5-en-3β-ol. The C29:2 and C30:1 unsaturated aliphatic alcohols were predominant. Saturated and monounsaturated C28 to C36n-alkyl diols occurred as a mixture of positional isomers with ω16 predominating among the C28 alkyl diols, ω18 among C30, C32, C34 and C36 alkyl diols and ω17 for the odd chain alkyl diols. C28–C32 hydroxy ketones were identified for the first time in a Marine Organism, with a distribution parallelling the alkyl diol mixtures and suggesting a biosynthetic relation between both alcohol classes. N. gaditana was fed to Brachionus plicatilis and the distribution of alkyl diol positional isomers remained the same in the algae and the rotifer fecal pellets. However, digestion resulted in an increase in C30 diols relative to C32 diols and in an enrichment of unsaturated long-chain alcohols relative to alkyl diols.
