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C.a.j.m. Jakobs - One of the best experts on this subject based on the ideXlab platform.

  • Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography.
    Journal of lipid research, 1992
    Co-Authors: H. J. Ten Brink, F. Stellaard, C. M. M. Van Den Heuvel, Rob Kok, D. S. M. Schor, R. J. A. Wanders, C.a.j.m. Jakobs
    Abstract:

    A sensitive and selective stable isotope dilution method was developed for the accurate quantitation of pris- tanic acid and phytanic acid using electron capture nega- tive ion Mass Fragmentography on pentafluorobenzyl derivatives. This technique allows detection of 1 pg of each compound and was applied to plasma from healthy controls and patients suffering from various peroxisomal disorders. The age-dependency of phytanic and pristanic acid levels in plasma from healthy controls was demonstrated. The invol- vement of peroxisomes in the fhxidation of pristanic acid was concluded from its accumulation in plasma from patients with peroxisomal deficiencies. Pristanic acid/ phytanic acid ratios were markedly increased in bifunction- al protein and/or SoxoacylCoA thiolase deficiency, indicat- ing their role in the (differential) diagnosis of disorders of peroxisomal fhxidation. - ten Brink, H.J., F. Stellaani, C.M.M. van den Hewel, RM. Ibk, D.S.M. Mor, RJA. Wandem, and C. Jakobs. Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography. J. Lipid Res. 1992. 33: 41-47.

Patrick Man Pan Yuen - One of the best experts on this subject based on the ideXlab platform.

  • Routine analysis of plasma busulfan by gas chromatography–Mass Fragmentography
    Clinical chemistry, 1998
    Co-Authors: Wai-kai Lai, Chi Pui Pang, Lap-kay Law, Raymond S. M. Wong, Patrick Man Pan Yuen
    Abstract:

    Busulfan (BU) is a widely used alkylating agent for antineoplastic therapy and marrow ablation in preparation for bone marrow transplantation (BMT). High-dose BU often leads to successful preparation and low relapse but is associated with veno-occlusive disease of liver. We established a protocol to determine postdosage plasma BU concentrations by gas chromatography–Mass Fragmentography in an attempt to relate clinical outcome to plasma BU concentrations. We used nonisotopic pusulfan as the internal standard. After extraction into ethyl acetate, BU and pusulfan were iodinated into 1,4-diiodobutane and 1,5-diiodopentane, respectively. Gas chromatography–Mass spectrometry (GC–MS) analysis was carried out on an Hewlett–Packard (HP) 5890II gas chromatograph with a 30-m 100% methyl silicon narrow bore, fused-silica capillary column interfaced with an HP 5970A Mass spectrometer. Helium was the carrier gas. The sample molecules were identified by total ion monitoring and quantified by selective ion monitoring of m/z 183 and 197. The calibration curve was linear to 4 mg/L. The limit of quantification was 0.04 mg/L, and the analytical recovery was ∼97%. The within-day and between-day imprecision (CV) was

  • routine analysis of plasma busulfan by gas chromatography Mass Fragmentography
    Clinical Chemistry, 1998
    Co-Authors: Wai-kai Lai, Chi Pui Pang, Lap-kay Law, Raymond S. M. Wong, Patrick Man Pan Yuen
    Abstract:

    Busulfan (BU) is a widely used alkylating agent for antineoplastic therapy and marrow ablation in preparation for bone marrow transplantation (BMT). High-dose BU often leads to successful preparation and low relapse but is associated with veno-occlusive disease of liver. We established a protocol to determine postdosage plasma BU concentrations by gas chromatography–Mass Fragmentography in an attempt to relate clinical outcome to plasma BU concentrations. We used nonisotopic pusulfan as the internal standard. After extraction into ethyl acetate, BU and pusulfan were iodinated into 1,4-diiodobutane and 1,5-diiodopentane, respectively. Gas chromatography–Mass spectrometry (GC–MS) analysis was carried out on an Hewlett–Packard (HP) 5890II gas chromatograph with a 30-m 100% methyl silicon narrow bore, fused-silica capillary column interfaced with an HP 5970A Mass spectrometer. Helium was the carrier gas. The sample molecules were identified by total ion monitoring and quantified by selective ion monitoring of m/z 183 and 197. The calibration curve was linear to 4 mg/L. The limit of quantification was 0.04 mg/L, and the analytical recovery was ∼97%. The within-day and between-day imprecision (CV) was

H. J. Ten Brink - One of the best experts on this subject based on the ideXlab platform.

  • Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography.
    Journal of lipid research, 1992
    Co-Authors: H. J. Ten Brink, F. Stellaard, C. M. M. Van Den Heuvel, Rob Kok, D. S. M. Schor, R. J. A. Wanders, C.a.j.m. Jakobs
    Abstract:

    A sensitive and selective stable isotope dilution method was developed for the accurate quantitation of pris- tanic acid and phytanic acid using electron capture nega- tive ion Mass Fragmentography on pentafluorobenzyl derivatives. This technique allows detection of 1 pg of each compound and was applied to plasma from healthy controls and patients suffering from various peroxisomal disorders. The age-dependency of phytanic and pristanic acid levels in plasma from healthy controls was demonstrated. The invol- vement of peroxisomes in the fhxidation of pristanic acid was concluded from its accumulation in plasma from patients with peroxisomal deficiencies. Pristanic acid/ phytanic acid ratios were markedly increased in bifunction- al protein and/or SoxoacylCoA thiolase deficiency, indicat- ing their role in the (differential) diagnosis of disorders of peroxisomal fhxidation. - ten Brink, H.J., F. Stellaani, C.M.M. van den Hewel, RM. Ibk, D.S.M. Mor, RJA. Wandem, and C. Jakobs. Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography. J. Lipid Res. 1992. 33: 41-47.

F. Stellaard - One of the best experts on this subject based on the ideXlab platform.

  • Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography.
    Journal of lipid research, 1992
    Co-Authors: H. J. Ten Brink, F. Stellaard, C. M. M. Van Den Heuvel, Rob Kok, D. S. M. Schor, R. J. A. Wanders, C.a.j.m. Jakobs
    Abstract:

    A sensitive and selective stable isotope dilution method was developed for the accurate quantitation of pris- tanic acid and phytanic acid using electron capture nega- tive ion Mass Fragmentography on pentafluorobenzyl derivatives. This technique allows detection of 1 pg of each compound and was applied to plasma from healthy controls and patients suffering from various peroxisomal disorders. The age-dependency of phytanic and pristanic acid levels in plasma from healthy controls was demonstrated. The invol- vement of peroxisomes in the fhxidation of pristanic acid was concluded from its accumulation in plasma from patients with peroxisomal deficiencies. Pristanic acid/ phytanic acid ratios were markedly increased in bifunction- al protein and/or SoxoacylCoA thiolase deficiency, indicat- ing their role in the (differential) diagnosis of disorders of peroxisomal fhxidation. - ten Brink, H.J., F. Stellaani, C.M.M. van den Hewel, RM. Ibk, D.S.M. Mor, RJA. Wandem, and C. Jakobs. Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography. J. Lipid Res. 1992. 33: 41-47.

C. M. M. Van Den Heuvel - One of the best experts on this subject based on the ideXlab platform.

  • Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography.
    Journal of lipid research, 1992
    Co-Authors: H. J. Ten Brink, F. Stellaard, C. M. M. Van Den Heuvel, Rob Kok, D. S. M. Schor, R. J. A. Wanders, C.a.j.m. Jakobs
    Abstract:

    A sensitive and selective stable isotope dilution method was developed for the accurate quantitation of pris- tanic acid and phytanic acid using electron capture nega- tive ion Mass Fragmentography on pentafluorobenzyl derivatives. This technique allows detection of 1 pg of each compound and was applied to plasma from healthy controls and patients suffering from various peroxisomal disorders. The age-dependency of phytanic and pristanic acid levels in plasma from healthy controls was demonstrated. The invol- vement of peroxisomes in the fhxidation of pristanic acid was concluded from its accumulation in plasma from patients with peroxisomal deficiencies. Pristanic acid/ phytanic acid ratios were markedly increased in bifunction- al protein and/or SoxoacylCoA thiolase deficiency, indicat- ing their role in the (differential) diagnosis of disorders of peroxisomal fhxidation. - ten Brink, H.J., F. Stellaani, C.M.M. van den Hewel, RM. Ibk, D.S.M. Mor, RJA. Wandem, and C. Jakobs. Pristanic acid and phytanic acid in plasma from patients with peroxisomal disorders: stable isotope dilution analysis with electron capture negative ion Mass Fragmentography. J. Lipid Res. 1992. 33: 41-47.