The Experts below are selected from a list of 33 Experts worldwide ranked by ideXlab platform

Joonhyung Lee - One of the best experts on this subject based on the ideXlab platform.

  • Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor
    MRS Online Proceedings Library, 2012
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

  • Sensitive and simultaneous detection of cardiac markers in human serum using surface acoustic wave immunosensor.
    Analytical chemistry, 2011
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration. We also investigated effects of increasing operating frequency o...

  • Real-time detection of airborne viruses on a Mass-Sensitive Device.
    Applied physics letters, 2008
    Co-Authors: Joonhyung Lee, Jaesung Jang, Dernir Akin, Cagri A. Savran, Rashid Bashir
    Abstract:

    We present real-time detection of airborne Vaccinia viruses using quartz crystal microbalance (QCM) in an integrated manner. Vaccinia viruses were aerosolized and neutralized using an electrospray aerosol generator, transported into the QCM chamber, and captured by a QCM crystal. The capture of the viruses on the QCM crystal resulted in frequency shifts proportional to the number of viruses. The capture rate varied linearly with the concentration of initial virus suspensions (8.5x10(8)-8.5x10(10) particlesml) at flow rates of 2.0 and 1.1 lmin. This work demonstrates the general potential of mass sensitive detection of nanoscale biological entities in air.

  • Ribozyme-mediated signal augmentation on a Mass-Sensitive biosensor.
    Journal of the American Chemical Society, 2006
    Co-Authors: Scott M. Knudsen, Joonhyung Lee, And Andrew D. Ellington, Cagri A. Savran
    Abstract:

    Mass-based detection methods such as the quartz crystal microbalance (QCM) offer an attractive option to label-based methods; however the sensitivity is generally lower by comparison. In particular, low-molecular-weight analytes can be difficult to detect based on mass addition alone. In this communication, we present the use of effector-dependent ribozymes (aptazymes) as reagents for augmenting small ligand detection on a Mass-Sensitive Device. Two distinct aptazymes were chosen:  an L1-ligase-based aptazyme (L1-Rev), which is activated by a small peptide (MW ≈ 2.4 kDa) from the HIV-1 Rev protein, and a hammerhead cleavase-based aptazyme (HH-theo3) activated by theophylline (MW = 180 Da). Aptazyme activity was observed in real time, and low-molecular-weight analyte detection has been successfully demonstrated with both aptazymes.

Soo Suk Lee - One of the best experts on this subject based on the ideXlab platform.

  • Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor
    MRS Online Proceedings Library, 2012
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

  • Sensitive and simultaneous detection of cardiac markers in human serum using surface acoustic wave immunosensor.
    Analytical chemistry, 2011
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration. We also investigated effects of increasing operating frequency o...

Preeda Prakrankamanant - One of the best experts on this subject based on the ideXlab platform.

  • Quartz crystal microbalance biosensors: prospects for point-of-care diagnostics.
    Journal of the Medical Association of Thailand Chotmaihet thangphaet, 2014
    Co-Authors: Preeda Prakrankamanant
    Abstract:

    Background: A QCM is a label-free and extremely Mass-Sensitive Device, which allows the detection of the binding event between trace medical analytes and bio-receptors on its surface. QCM, the most promising type of biosensors, has attracted much interest due to the inherent benefits over other transducers, including better sensitivity, ease-of-use, integration with compact analytical Devices, and economy, and also involving relatively simple technology in its production. Thus, they have great potential with regard to point-of-care (POC) testing for early detection of diseases. Material and Method: Retrievable articles that related to acoustic type sensing of Pubmed and Science direct database were included. Additionally, abstracts presented at Biosensor World Congress held between 2008 and 2012 were searching to identify relevant clinical trials. Results: All studies demonstrated the opportunity in the use of QCM as a novel diagnostic method. Several attempts have been made to construct integrated systems that show promising application for POC tests. Conclusion: This review represents another step to meet challenges, especially in the improved minimization and sensitivity of biosensors. As this work continues, new bioreceptor and biomarkers emerging from the could make it an ideal candidate for cheap POC diagnostic. Keywords: Quartz crystal microbalance, Biosensor, Point-of-care testing

  • Quartz crystal microbalance biosensors: prospects for point-of-care diagnostics.
    Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 2014
    Co-Authors: Preeda Prakrankamanant
    Abstract:

    A QCM is a label-free and extremely Mass-Sensitive Device, which allows the detection of the binding event between trace medical analytes and bio-receptors on its surface. QCM, the most promising type of biosensors, has attracted much interest due to the inherent benefits over other transducers, including better sensitivity, ease-of-use, integration with compact analytical Devices, and economy, and also involving relatively simple technology in its production. Thus, they have great potential with regard to point-of-care (POC) testing for early detection of diseases. Retrievable articles that related to acoustic type sensing of Pubmed and Science direct database were included. Additionally, abstracts presented at Biosensor World Congress held between 2008 and 2012 were searching to identify relevant clinical trials. All studies demonstrated the opportunity in the use of QCM as a novel diagnostic method. Several attempts have been made to construct integrated systems that show promising application for POC tests. This review represents another step to meet challenges, especially in the improved minimization and sensitivity of biosensors. As this work continues, new bioreceptor and biomarkers emerging from the could make it an ideal candidate for cheap POC diagnostic.

Yeolho Lee - One of the best experts on this subject based on the ideXlab platform.

  • Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor
    MRS Online Proceedings Library, 2012
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

  • Sensitive and simultaneous detection of cardiac markers in human serum using surface acoustic wave immunosensor.
    Analytical chemistry, 2011
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration. We also investigated effects of increasing operating frequency o...

Eun Chol Cho - One of the best experts on this subject based on the ideXlab platform.

  • Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor
    MRS Online Proceedings Library, 2012
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

  • Sensitive and simultaneous detection of cardiac markers in human serum using surface acoustic wave immunosensor.
    Analytical chemistry, 2011
    Co-Authors: Joonhyung Lee, Youn-suk Choi, Yeolho Lee, Hun Joo Lee, Jung Nam Lee, Sang Kyu Kim, Kyung Yeon Han, Eun Chol Cho, Jae Chan Park, Soo Suk Lee
    Abstract:

    We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a Mass-Sensitive Device. The sensor response due to gold staining varied as a function of cardiac marker concentration. We also investigated effects of increasing operating frequency o...