The Experts below are selected from a list of 760062 Experts worldwide ranked by ideXlab platform
Myung Ho Jeong - One of the best experts on this subject based on the ideXlab platform.
-
thromboresistant and endothelialization effects of dopamine mediated heparin coating on a stent Material Surface
Journal of Materials Science: Materials in Medicine, 2012Co-Authors: Inkyu Park, Dae Sung Park, Myung Ho JeongAbstract:Heparinization of Surfaces has proven a successful strategy to prevent thrombus formation. Inspired by the composition of adhesive proteins in mussels, the authors used dopamine to immobilize heparin on a stent Surface. This study aimed to assess the thromboresistant and endothelialization effects of dopamine-mediated heparin (HPM) coating on a stent Material Surface. The HPM was synthesized by bonding dopamine and heparin chemically. Cobalt–chromium (Co–Cr) alloy disks were first placed in the HPM solution and applied to Surface stability then underwent thromboresistant tests and human umbilical vein endothelial cells (HUVEC) cytotoxicity assays. The results showed not only thromboresistant activity and a stable state of heparin on the Surfaces after investigation with toluidine blue and thrombin activation assay but also proliferation of HUVEC in vitro. Studies on animals showed that the HPM-coated stent has no obvious inflammation response and increasing of restenosis rate compared to the bare metal stent (BMS) indicating good biocompatibility as well as safety in its in vivo application. Moreover, improving the endothelial cell (EC) proliferation resulted in a higher strut-covering rate (i.e., endothelialization) with shuttle-shaped EC in the HPM-coated stent group compared to that of the BMS group. These results suggest that this facile coating approach could significantly promote endothelialization and offer greater safety than the BMS for its much improved thromboresistant property. Moreover, it may offer a platform for conjugating secondary drugs such as anti-proliferative drugs.
-
thromboresistant and endothelialization effects of dopamine mediated heparin coating on a stent Material Surface
Journal of Materials Science: Materials in Medicine, 2012Co-Authors: Inkyu Park, Dae Sung Park, Myung Ho JeongAbstract:Heparinization of Surfaces has proven a successful strategy to prevent thrombus formation. Inspired by the composition of adhesive proteins in mussels, the authors used dopamine to immobilize heparin on a stent Surface. This study aimed to assess the thromboresistant and endothelialization effects of dopamine-mediated heparin (HPM) coating on a stent Material Surface. The HPM was synthesized by bonding dopamine and heparin chemically. Cobalt–chromium (Co–Cr) alloy disks were first placed in the HPM solution and applied to Surface stability then underwent thromboresistant tests and human umbilical vein endothelial cells (HUVEC) cytotoxicity assays. The results showed not only thromboresistant activity and a stable state of heparin on the Surfaces after investigation with toluidine blue and thrombin activation assay but also proliferation of HUVEC in vitro. Studies on animals showed that the HPM-coated stent has no obvious inflammation response and increasing of restenosis rate compared to the bare metal stent (BMS) indicating good biocompatibility as well as safety in its in vivo application. Moreover, improving the endothelial cell (EC) proliferation resulted in a higher strut-covering rate (i.e., endothelialization) with shuttle-shaped EC in the HPM-coated stent group compared to that of the BMS group. These results suggest that this facile coating approach could significantly promote endothelialization and offer greater safety than the BMS for its much improved thromboresistant property. Moreover, it may offer a platform for conjugating secondary drugs such as anti-proliferative drugs.
Aj Harvie - One of the best experts on this subject based on the ideXlab platform.
-
screening and characterisation of cdte cds quantum dot binding peptides for Material Surface functionalisation
RSC Advances, 2020Co-Authors: Thanawat Suwatthanarak, Kevin Critchley, Stephen D. Evans, Aj Harvie, Masayoshi Tanaka, Taisuke Minamide, Abiral Tamang, Mina OkochiAbstract:Quantum dots (QDs) are promising nanoMaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle Surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD Surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD Surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD Surface functionalisation in biological applications.
-
Screening and characterisation of CdTe/CdS quantum dot-binding peptides for Material Surface functionalisation
'Royal Society of Chemistry (RSC)', 2020Co-Authors: Suwatthanarak T, Tanaka M, Minamide T, Aj Harvie, Tamang A, Critchley K, Sd Evans, Okochi MAbstract:Quantum dots (QDs) are promising nanoMaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle Surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD Surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD Surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD Surface functionalisation in biological applications
Mina Okochi - One of the best experts on this subject based on the ideXlab platform.
-
screening and characterisation of cdte cds quantum dot binding peptides for Material Surface functionalisation
RSC Advances, 2020Co-Authors: Thanawat Suwatthanarak, Kevin Critchley, Stephen D. Evans, Aj Harvie, Masayoshi Tanaka, Taisuke Minamide, Abiral Tamang, Mina OkochiAbstract:Quantum dots (QDs) are promising nanoMaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle Surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD Surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD Surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD Surface functionalisation in biological applications.
Inkyu Park - One of the best experts on this subject based on the ideXlab platform.
-
thromboresistant and endothelialization effects of dopamine mediated heparin coating on a stent Material Surface
Journal of Materials Science: Materials in Medicine, 2012Co-Authors: Inkyu Park, Dae Sung Park, Myung Ho JeongAbstract:Heparinization of Surfaces has proven a successful strategy to prevent thrombus formation. Inspired by the composition of adhesive proteins in mussels, the authors used dopamine to immobilize heparin on a stent Surface. This study aimed to assess the thromboresistant and endothelialization effects of dopamine-mediated heparin (HPM) coating on a stent Material Surface. The HPM was synthesized by bonding dopamine and heparin chemically. Cobalt–chromium (Co–Cr) alloy disks were first placed in the HPM solution and applied to Surface stability then underwent thromboresistant tests and human umbilical vein endothelial cells (HUVEC) cytotoxicity assays. The results showed not only thromboresistant activity and a stable state of heparin on the Surfaces after investigation with toluidine blue and thrombin activation assay but also proliferation of HUVEC in vitro. Studies on animals showed that the HPM-coated stent has no obvious inflammation response and increasing of restenosis rate compared to the bare metal stent (BMS) indicating good biocompatibility as well as safety in its in vivo application. Moreover, improving the endothelial cell (EC) proliferation resulted in a higher strut-covering rate (i.e., endothelialization) with shuttle-shaped EC in the HPM-coated stent group compared to that of the BMS group. These results suggest that this facile coating approach could significantly promote endothelialization and offer greater safety than the BMS for its much improved thromboresistant property. Moreover, it may offer a platform for conjugating secondary drugs such as anti-proliferative drugs.
-
thromboresistant and endothelialization effects of dopamine mediated heparin coating on a stent Material Surface
Journal of Materials Science: Materials in Medicine, 2012Co-Authors: Inkyu Park, Dae Sung Park, Myung Ho JeongAbstract:Heparinization of Surfaces has proven a successful strategy to prevent thrombus formation. Inspired by the composition of adhesive proteins in mussels, the authors used dopamine to immobilize heparin on a stent Surface. This study aimed to assess the thromboresistant and endothelialization effects of dopamine-mediated heparin (HPM) coating on a stent Material Surface. The HPM was synthesized by bonding dopamine and heparin chemically. Cobalt–chromium (Co–Cr) alloy disks were first placed in the HPM solution and applied to Surface stability then underwent thromboresistant tests and human umbilical vein endothelial cells (HUVEC) cytotoxicity assays. The results showed not only thromboresistant activity and a stable state of heparin on the Surfaces after investigation with toluidine blue and thrombin activation assay but also proliferation of HUVEC in vitro. Studies on animals showed that the HPM-coated stent has no obvious inflammation response and increasing of restenosis rate compared to the bare metal stent (BMS) indicating good biocompatibility as well as safety in its in vivo application. Moreover, improving the endothelial cell (EC) proliferation resulted in a higher strut-covering rate (i.e., endothelialization) with shuttle-shaped EC in the HPM-coated stent group compared to that of the BMS group. These results suggest that this facile coating approach could significantly promote endothelialization and offer greater safety than the BMS for its much improved thromboresistant property. Moreover, it may offer a platform for conjugating secondary drugs such as anti-proliferative drugs.
Thanawat Suwatthanarak - One of the best experts on this subject based on the ideXlab platform.
-
screening and characterisation of cdte cds quantum dot binding peptides for Material Surface functionalisation
RSC Advances, 2020Co-Authors: Thanawat Suwatthanarak, Kevin Critchley, Stephen D. Evans, Aj Harvie, Masayoshi Tanaka, Taisuke Minamide, Abiral Tamang, Mina OkochiAbstract:Quantum dots (QDs) are promising nanoMaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle Surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD Surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD Surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD Surface functionalisation in biological applications.
