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Juan M. Zapata - One of the best experts on this subject based on the ideXlab platform.
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Phylogeny of the TRAF/MATH Domain.
Advances in experimental medicine and biology, 2007Co-Authors: Juan M. Zapata, Vanesa Martínez-garcía, Sophie LefebvreAbstract:The TNF-receptor associated factor (TRAF) Domain (TD), also known as the meprin and TRAF-C homology (MATH) Domain is a fold of seven anti-parallel p-helices that participates in protein-protein interactions. This fold is broadly represented among eukaryotes, where it is found associated with a discrete set of protein-Domains. Virtually all protein families encompassing a TRAF/MATH Domain seem to be involved in the regulation of protein processing and ubiquitination, strongly suggesting a parallel evolution of the TRAF/MATH Domain and certain proteolysis pathways in eukaryotes. The restricted number of living organisms for which we have information of their genetic and protein make-up limits the scope and analysis of the MATH Domain in evolution. However, the available information allows us to get a glimpse on the origins, distribution and evolution of the TRAF/MATH Domain, which will be overviewed in this chapter.
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phylogeny of the traf MATH Domain
Advances in Experimental Medicine and Biology, 2007Co-Authors: Juan M. Zapata, Vanesa Martinezgarcia, Sophie LefebvreAbstract:The TNF-receptor associated factor (TRAF) Domain (TD), also known as the meprin and TRAF-C homology (MATH) Domain is a fold of seven anti-parallel p-helices that participates in protein-protein interactions. This fold is broadly represented among eukaryotes, where it is found associated with a discrete set of protein-Domains. Virtually all protein families encompassing a TRAF/MATH Domain seem to be involved in the regulation of protein processing and ubiquitination, strongly suggesting a parallel evolution of the TRAF/MATH Domain and certain proteolysis pathways in eukaryotes. The restricted number of living organisms for which we have information of their genetic and protein make-up limits the scope and analysis of the MATH Domain in evolution. However, the available information allows us to get a glimpse on the origins, distribution and evolution of the TRAF/MATH Domain, which will be overviewed in this chapter.
Sophie Lefebvre - One of the best experts on this subject based on the ideXlab platform.
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Phylogeny of the TRAF/MATH Domain.
Advances in experimental medicine and biology, 2007Co-Authors: Juan M. Zapata, Vanesa Martínez-garcía, Sophie LefebvreAbstract:The TNF-receptor associated factor (TRAF) Domain (TD), also known as the meprin and TRAF-C homology (MATH) Domain is a fold of seven anti-parallel p-helices that participates in protein-protein interactions. This fold is broadly represented among eukaryotes, where it is found associated with a discrete set of protein-Domains. Virtually all protein families encompassing a TRAF/MATH Domain seem to be involved in the regulation of protein processing and ubiquitination, strongly suggesting a parallel evolution of the TRAF/MATH Domain and certain proteolysis pathways in eukaryotes. The restricted number of living organisms for which we have information of their genetic and protein make-up limits the scope and analysis of the MATH Domain in evolution. However, the available information allows us to get a glimpse on the origins, distribution and evolution of the TRAF/MATH Domain, which will be overviewed in this chapter.
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phylogeny of the traf MATH Domain
Advances in Experimental Medicine and Biology, 2007Co-Authors: Juan M. Zapata, Vanesa Martinezgarcia, Sophie LefebvreAbstract:The TNF-receptor associated factor (TRAF) Domain (TD), also known as the meprin and TRAF-C homology (MATH) Domain is a fold of seven anti-parallel p-helices that participates in protein-protein interactions. This fold is broadly represented among eukaryotes, where it is found associated with a discrete set of protein-Domains. Virtually all protein families encompassing a TRAF/MATH Domain seem to be involved in the regulation of protein processing and ubiquitination, strongly suggesting a parallel evolution of the TRAF/MATH Domain and certain proteolysis pathways in eukaryotes. The restricted number of living organisms for which we have information of their genetic and protein make-up limits the scope and analysis of the MATH Domain in evolution. However, the available information allows us to get a glimpse on the origins, distribution and evolution of the TRAF/MATH Domain, which will be overviewed in this chapter.
Manuel Rubio - One of the best experts on this subject based on the ideXlab platform.
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gene expression analysis of plum pox virus sharka susceptibility resistance in apricot prunus armeniaca l
PLOS ONE, 2015Co-Authors: Manuel Rubio, Anarosa Ballester, Pedro Manuel Olivares, Manuel Castro De Moura, F Dicenta, Pedro MartinezgomezAbstract:RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV infection in leaves from two full-sib apricot genotypes, “Rojo Pasion” and “Z506-7”, resistant and susceptible to PPV, respectively. Transcriptomic analyses revealed the existence of more than 2,000 genes related to the pathogen response and resistance to PPV in apricot. These results showed that the response to infection by the virus in the susceptible genotype is associated with an induction of genes involved in pathogen resistance such as the allene oxide synthase, S-adenosylmethionine synthetase 2 and the major MLP-like protein 423. Over-expression of the Dicer protein 2a may indicate the suppression of a gene silencing mechanism of the plant by PPV HCPro and P1 PPV proteins. On the other hand, there were 164 genes involved in resistance mechanisms that have been identified in apricot, 49 of which are located in the PPVres region (scaffold 1 positions from 8,050,804 to 8,244,925), which is responsible for PPV resistance in apricot. Among these genes in apricot there are several MATH Domain-containing genes, although other genes inside (Pleiotropic drug resistance 9 gene) or outside (CAP, Cysteine-rich secretory proteins, Antigen 5 and Pathogenesis-related 1 protein; and LEA, Late embryogenesis abundant protein) PPVres region could also be involved in the resistance.
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Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot (Prunus armeniaca L.).
PloS one, 2015Co-Authors: Manuel Rubio, Anarosa Ballester, Pedro Manuel Olivares, Manuel Castro De Moura, F Dicenta, Pedro Martínez-gómezAbstract:RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV infection in leaves from two full-sib apricot genotypes, “Rojo Pasion” and “Z506-7”, resistant and susceptible to PPV, respectively. Transcriptomic analyses revealed the existence of more than 2,000 genes related to the pathogen response and resistance to PPV in apricot. These results showed that the response to infection by the virus in the susceptible genotype is associated with an induction of genes involved in pathogen resistance such as the allene oxide synthase, S-adenosylmethionine synthetase 2 and the major MLP-like protein 423. Over-expression of the Dicer protein 2a may indicate the suppression of a gene silencing mechanism of the plant by PPV HCPro and P1 PPV proteins. On the other hand, there were 164 genes involved in resistance mechanisms that have been identified in apricot, 49 of which are located in the PPVres region (scaffold 1 positions from 8,050,804 to 8,244,925), which is responsible for PPV resistance in apricot. Among these genes in apricot there are several MATH Domain-containing genes, although other genes inside (Pleiotropic drug resistance 9 gene) or outside (CAP, Cysteine-rich secretory proteins, Antigen 5 and Pathogenesis-related 1 protein; and LEA, Late embryogenesis abundant protein) PPVres region could also be involved in the resistance.
Shaoping Weng - One of the best experts on this subject based on the ideXlab platform.
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litopenaeus vannamei tumor necrosis factor receptor associated factor 6 traf6 responds to vibrio alginolyticus and white spot syndrome virus wssv infection and activates antimicrobial peptide genes
Developmental and Comparative Immunology, 2011Co-Authors: Peihui Wang, Dinghui Wan, Xiexiong Deng, Shaoping WengAbstract:Abstract Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) is a key signaling adaptor protein not only for the TNFR superfamily but also for the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. To investigate TRAF6 function in invertebrate innate immune responses, Litopenaeus vannamei TRAF6 (LvTRAF6) was identified and characterized. The full-length cDNA of LvTRAF6 is 2823 bp long, with an open reading frame (ORF) encoding a putative protein of 594 amino acids, including a RING-type Zinc finger, two TRAF-type Zinc fingers, a coiled–coil region, and a meprin and TRAF homology (MATH) Domain. The overall amino acid sequence identity between LvTRAF6 and other known TRAF6s is 22.2–33.3%. Dual luciferase reporter assays in Drosophila S2 cells revealed that LvTRAF6 could activate the promoters of antimicrobial peptide genes (AMPs), including Drosophila Attacin A and Drosomycin, and shrimp Penaeidins. Real-time quantitative PCR (qPCR) indicated that LvTRAF6 was constitutively expressed in various tissues of L. vannamei. After Vibrio alginolyticus and white spot syndrome virus (WSSV) challenge, LvTRAF6 was down-regulated, though with different expression patterns in the intestine compared to other tissues. After WSSV challenge, LvTRAF6 was up-regulated 2.7- and 2.3-fold over the control at 3 h in gills and hepatopancreas, respectively. These results indicated that LvTRAF6 may play a crucial role in antibacterial and antiviral responses via regulation of AMP gene expression.
Qiwei Qin - One of the best experts on this subject based on the ideXlab platform.
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TRAF6 is a critical factor in fish immune response to virus infection.
Fish & shellfish immunology, 2016Co-Authors: Jingguang Wei, Shaoqing Zang, Qiaojun Zheng, Xiuli Chen, Qiwei QinAbstract:Tumor necrosis factor receptor-associated factor 6 (TRAF6) is one of the key adaptor molecule in Toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. In our previous study, the molecular characteristics of EtTRAF6 (TRAF6 from Epinephelus tauvina), the tissue distributions, expression patterns after challenging with bacterial and viral pathogens were investigated. Here we identified EtTRAF6 as an important regulator of virus-triggered signaling pathway. Overexpression of EtTRAF6-ORF and truncated forms of EtTRAF6, including EtTRAF6-C (delete the MATH Domain), EtTRAF6-N (delete the RING Domain) and EtTRAF6-MATH, inhibited IFN-β activity strongly in grouper spleen (GS) cells. Overexpression of EtTRAF6 repressed virus-induced production of type I IFNs. When EtTRAF6 cotransfected with EcIRF3 or EcIRF7, EtTRAF6 inhibited IRF-induced activation of IFN-β. Over-expressed EtTRAF6 inhibited the transcription of SGIV genes significantly in GS cells. Although TRAF6 has a role in apoptosis regulation, it is not known if EtTRAF6 has any role in apoptosis regulation. Strikingly, when over-expressed in fathead minnow (FHM) cells, EtTRAF6 protected them from cell death induced by SGIV. Therefore, these results suggest that TRAF6 may play a critical role in their response to SGIV infection, through regulation of a cell death pathway that is common to fish and humans.
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isolation and characterization of tumor necrosis factor receptor associated factor 6 traf6 from grouper epinephelus tauvina
Fish & Shellfish Immunology, 2014Co-Authors: Jingguang Wei, Minglan Guo, Pin Gao, Yang Yan, Qiwei QinAbstract:Tumor necrosis factor receptor-associated factor 6 (TRAF6) is one of the key adapter molecules in Toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. In the present study, a TRAF6 (named as Et-TRAF6) was identified from the marine fish grouper, Epinephelus tauvina by RACE PCR. The full-length cDNA of Et-TRAF6 comprised 1949 bp with a 1713 bp open reading frame (ORF) that encodes a putative protein of 570 amino acids. Similar to most TRAF6s, Et-TRAF6 includes one N-terminal RING Domain (78aa-116aa), two zinc fingers of TRAF-type (159aa-210aa and 212aa-269aa), one coiled-coil region (370aa-394aa), and one conserved C-terminal meprin and TRAF homology (MATH) Domain (401aa-526aa). Quantitative real-time PCR analysis revealed that Et-TRAF6 mRNA is expressed in all tested tissues, with the predominant expression in the stomach and intestine. The expression of Et-TRAF6 was up-regulated in the liver after challenge with Lipoteichoic acid (LTA), Peptidoglycan (PGN), Zymosan, polyinosine polycytidylic acid [Poly(I:C)] and Polydeoxyadenylic acid -Polythymidylic acid sodium salt [Poly(dA:dT)]. The expression of Et-TRAF6 was also up-regulated in the liver after infection with Vibrio alginolyticus, Singapore grouper iridovirus (SGIV) and grouper nervous necrosis virus (GNNV). Recombinant Et-TRAF6 (rEt-TRAF6) was expressed in Escherichia BL21 (DE3) and purified for mouse anti-Et-TRAF6 serum preparation. Intracellular localization revealed that Et-TRAF6 is distributed in both cytoplasm and nucleus, and predominantly in the cytoplasm. These results together indicated that Et-TRAF6 might be involved in immune responses toward bacterial and virus challenges. (C) 2014 Elsevier Ltd. All rights reserved.
