The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Adam J Engler - One of the best experts on this subject based on the ideXlab platform.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Recent work has proposed that both protein tethering to the extracellular Matrix and Matrix Porosity can regulate stem cell differentiation. It is now shown that differentiation is driven by Matrix stiffness independently of tethering and Porosity.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Stem cells regulate their fate by binding to, and contracting against, the extracellular Matrix. Recently, it has been proposed that in addition to Matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and Matrix Porosity, also regulates stem cell differentiation. By modulating substrate Porosity without altering stiffness in polyacrylamide gels, we show that varying substrate Porosity did not significantly change protein tethering, substrate deformations, or the osteogenic and adipogenic differentiation of human adipose-derived stromal cells and marrow-derived mesenchymal stromal cells. Varying protein-substrate linker density up to 50-fold changed tethering, but did not affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations. Differentiation was also unaffected by the absence of protein tethering. Our findings imply that the stiffness of planar matrices regulates stem cell differentiation independently of protein tethering and Porosity.
Yu Suk Choi - One of the best experts on this subject based on the ideXlab platform.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Recent work has proposed that both protein tethering to the extracellular Matrix and Matrix Porosity can regulate stem cell differentiation. It is now shown that differentiation is driven by Matrix stiffness independently of tethering and Porosity.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Stem cells regulate their fate by binding to, and contracting against, the extracellular Matrix. Recently, it has been proposed that in addition to Matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and Matrix Porosity, also regulates stem cell differentiation. By modulating substrate Porosity without altering stiffness in polyacrylamide gels, we show that varying substrate Porosity did not significantly change protein tethering, substrate deformations, or the osteogenic and adipogenic differentiation of human adipose-derived stromal cells and marrow-derived mesenchymal stromal cells. Varying protein-substrate linker density up to 50-fold changed tethering, but did not affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations. Differentiation was also unaffected by the absence of protein tethering. Our findings imply that the stiffness of planar matrices regulates stem cell differentiation independently of protein tethering and Porosity.
Ludovic G Vincent - One of the best experts on this subject based on the ideXlab platform.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Recent work has proposed that both protein tethering to the extracellular Matrix and Matrix Porosity can regulate stem cell differentiation. It is now shown that differentiation is driven by Matrix stiffness independently of tethering and Porosity.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Stem cells regulate their fate by binding to, and contracting against, the extracellular Matrix. Recently, it has been proposed that in addition to Matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and Matrix Porosity, also regulates stem cell differentiation. By modulating substrate Porosity without altering stiffness in polyacrylamide gels, we show that varying substrate Porosity did not significantly change protein tethering, substrate deformations, or the osteogenic and adipogenic differentiation of human adipose-derived stromal cells and marrow-derived mesenchymal stromal cells. Varying protein-substrate linker density up to 50-fold changed tethering, but did not affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations. Differentiation was also unaffected by the absence of protein tethering. Our findings imply that the stiffness of planar matrices regulates stem cell differentiation independently of protein tethering and Porosity.
Hermes Taylorweiner - One of the best experts on this subject based on the ideXlab platform.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Recent work has proposed that both protein tethering to the extracellular Matrix and Matrix Porosity can regulate stem cell differentiation. It is now shown that differentiation is driven by Matrix stiffness independently of tethering and Porosity.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Stem cells regulate their fate by binding to, and contracting against, the extracellular Matrix. Recently, it has been proposed that in addition to Matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and Matrix Porosity, also regulates stem cell differentiation. By modulating substrate Porosity without altering stiffness in polyacrylamide gels, we show that varying substrate Porosity did not significantly change protein tethering, substrate deformations, or the osteogenic and adipogenic differentiation of human adipose-derived stromal cells and marrow-derived mesenchymal stromal cells. Varying protein-substrate linker density up to 50-fold changed tethering, but did not affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations. Differentiation was also unaffected by the absence of protein tethering. Our findings imply that the stiffness of planar matrices regulates stem cell differentiation independently of protein tethering and Porosity.
Shaochen Chen - One of the best experts on this subject based on the ideXlab platform.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Recent work has proposed that both protein tethering to the extracellular Matrix and Matrix Porosity can regulate stem cell differentiation. It is now shown that differentiation is driven by Matrix stiffness independently of tethering and Porosity.
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interplay of Matrix stiffness and protein tethering in stem cell differentiation
Nature Materials, 2014Co-Authors: Ludovic G Vincent, Alexander Fuhrmann, Yu Suk Choi, Kolin C Hribar, Hermes Taylorweiner, Shaochen Chen, Adam J EnglerAbstract:Stem cells regulate their fate by binding to, and contracting against, the extracellular Matrix. Recently, it has been proposed that in addition to Matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and Matrix Porosity, also regulates stem cell differentiation. By modulating substrate Porosity without altering stiffness in polyacrylamide gels, we show that varying substrate Porosity did not significantly change protein tethering, substrate deformations, or the osteogenic and adipogenic differentiation of human adipose-derived stromal cells and marrow-derived mesenchymal stromal cells. Varying protein-substrate linker density up to 50-fold changed tethering, but did not affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations. Differentiation was also unaffected by the absence of protein tethering. Our findings imply that the stiffness of planar matrices regulates stem cell differentiation independently of protein tethering and Porosity.