The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform

Yong-dae Kwon - One of the best experts on this subject based on the ideXlab platform.

  • Bisphosphonates hinder osteoblastic/osteoclastic differentiation in the Maxillary Sinus Mucosa-derived stem cells
    Clinical Oral Investigations, 2018
    Co-Authors: Jun Zhang, Jaesuh Park, Yong-dae Kwon
    Abstract:

    Objectives Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human Maxillary Sinus Mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved. Materials and methods The effects of ZOL and ALN were assessed for osteoblast differentiation by alkaline phosphatase (ALP) activity, alizarin red staining, and RT-PCR for genes encoding Runx2 and osterix. Receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclast differentiation in bone marrow macrophages (BMMs) was also examined. Results ZOL and ALN both suppressed osteoblastic differentiation, as evidenced by their effects on ALP activity, mineralization nodule formation, and the mRNA expression levels of osteoblastic transcript factors. The RANKL/osteoprotegerin ratio in HMSMCs was increased by ALN, whereas ZOL had the opposite effect. Conditioned medium obtained from ALN-treated HMSMCs stimulated osteoclast formation and upregulated NFATc1 expression, whereas conditioned medium from ZOL-treated cells did not. ALN was more cytotoxic and stimulated apoptosis more strongly than ZOL. BPs decreased the protein levels of the non-canonical Wnt signaling protein Wnt5a and calmodulin-dependent kinase II. Moreover, recombinant human Wnt5a reversed the effects of BPs on osteoblastic and osteoclastic differentiation. Conclusion This study is the first demonstration that BPs exert negative effects on osteoblastic and osteoclastic processes via the non-canonical Wnt pathway in HMSMSCs. Clinical relevance It suggests that patients taking BPs during the period of Maxillary Sinus lifting and amentation should be given special attention.

  • bisphosphonates hinder osteoblastic osteoclastic differentiation in the Maxillary Sinus Mucosa derived stem cells
    Clinical Oral Investigations, 2018
    Co-Authors: Jun Zhang, Jaesuh Park, Yong-dae Kwon
    Abstract:

    Objectives Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human Maxillary Sinus Mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved.

Jun Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Bisphosphonates hinder osteoblastic/osteoclastic differentiation in the Maxillary Sinus Mucosa-derived stem cells
    Clinical Oral Investigations, 2018
    Co-Authors: Jun Zhang, Jaesuh Park, Yong-dae Kwon
    Abstract:

    Objectives Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human Maxillary Sinus Mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved. Materials and methods The effects of ZOL and ALN were assessed for osteoblast differentiation by alkaline phosphatase (ALP) activity, alizarin red staining, and RT-PCR for genes encoding Runx2 and osterix. Receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclast differentiation in bone marrow macrophages (BMMs) was also examined. Results ZOL and ALN both suppressed osteoblastic differentiation, as evidenced by their effects on ALP activity, mineralization nodule formation, and the mRNA expression levels of osteoblastic transcript factors. The RANKL/osteoprotegerin ratio in HMSMCs was increased by ALN, whereas ZOL had the opposite effect. Conditioned medium obtained from ALN-treated HMSMCs stimulated osteoclast formation and upregulated NFATc1 expression, whereas conditioned medium from ZOL-treated cells did not. ALN was more cytotoxic and stimulated apoptosis more strongly than ZOL. BPs decreased the protein levels of the non-canonical Wnt signaling protein Wnt5a and calmodulin-dependent kinase II. Moreover, recombinant human Wnt5a reversed the effects of BPs on osteoblastic and osteoclastic differentiation. Conclusion This study is the first demonstration that BPs exert negative effects on osteoblastic and osteoclastic processes via the non-canonical Wnt pathway in HMSMSCs. Clinical relevance It suggests that patients taking BPs during the period of Maxillary Sinus lifting and amentation should be given special attention.

  • bisphosphonates hinder osteoblastic osteoclastic differentiation in the Maxillary Sinus Mucosa derived stem cells
    Clinical Oral Investigations, 2018
    Co-Authors: Jun Zhang, Jaesuh Park, Yong-dae Kwon
    Abstract:

    Objectives Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human Maxillary Sinus Mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved.

Jaesuh Park - One of the best experts on this subject based on the ideXlab platform.

  • Bisphosphonates hinder osteoblastic/osteoclastic differentiation in the Maxillary Sinus Mucosa-derived stem cells
    Clinical Oral Investigations, 2018
    Co-Authors: Jun Zhang, Jaesuh Park, Yong-dae Kwon
    Abstract:

    Objectives Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human Maxillary Sinus Mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved. Materials and methods The effects of ZOL and ALN were assessed for osteoblast differentiation by alkaline phosphatase (ALP) activity, alizarin red staining, and RT-PCR for genes encoding Runx2 and osterix. Receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclast differentiation in bone marrow macrophages (BMMs) was also examined. Results ZOL and ALN both suppressed osteoblastic differentiation, as evidenced by their effects on ALP activity, mineralization nodule formation, and the mRNA expression levels of osteoblastic transcript factors. The RANKL/osteoprotegerin ratio in HMSMCs was increased by ALN, whereas ZOL had the opposite effect. Conditioned medium obtained from ALN-treated HMSMCs stimulated osteoclast formation and upregulated NFATc1 expression, whereas conditioned medium from ZOL-treated cells did not. ALN was more cytotoxic and stimulated apoptosis more strongly than ZOL. BPs decreased the protein levels of the non-canonical Wnt signaling protein Wnt5a and calmodulin-dependent kinase II. Moreover, recombinant human Wnt5a reversed the effects of BPs on osteoblastic and osteoclastic differentiation. Conclusion This study is the first demonstration that BPs exert negative effects on osteoblastic and osteoclastic processes via the non-canonical Wnt pathway in HMSMSCs. Clinical relevance It suggests that patients taking BPs during the period of Maxillary Sinus lifting and amentation should be given special attention.

  • bisphosphonates hinder osteoblastic osteoclastic differentiation in the Maxillary Sinus Mucosa derived stem cells
    Clinical Oral Investigations, 2018
    Co-Authors: Jun Zhang, Jaesuh Park, Yong-dae Kwon
    Abstract:

    Objectives Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human Maxillary Sinus Mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved.

Yanfeng Li - One of the best experts on this subject based on the ideXlab platform.

  • ex vivo comparative study on three Sinus lift tools for transcrestal detaching Maxillary Sinus Mucosa
    Bioengineered bugs, 2017
    Co-Authors: Yanfeng Li, Pin Hu, Xinming Dong, Chunhao Yang
    Abstract:

    ABSTRACTThe objective of this study was to comparatively evaluate 3 different Sinus lift tools, namely umbrella-shaped Sinus lift curette YSL-04, our recently designed probe-improved Sinus lift curettes, and our newly invented elevator 014, using our previous developed goat ex vivo models for direct visualizing the effectiveness of detaching Sinus Mucosa in real time. Goat ex vivo models for direct visualizing the effectiveness of detaching Sinus Mucosa in real time were generated according to our previously developed protocol. The effectiveness for each tool was evaluated through the length of Sinus Mucosa detached in mesial and distal directions or buccal and palatal directions, and the space volume created by detaching Maxillary Sinus Mucosa in mesial, distal, buccal and palatal directions. The results showed that all 3 Sinus lift tools could transcrestally detach the Maxillary Sinus Mucosa and create extra space under the elevated Sinus floor on the goat ex vivo Sinus models. Moreover, our newly inven...

  • feasibility of shape memory ni ti alloy wire containing tube elevators for transcrestal detaching Maxillary Sinus Mucosa ex vivo study
    Cellular Physiology and Biochemistry, 2016
    Co-Authors: Yanfeng Li, Fuli Wang, Pin Hu, Chunhao Yang, Xiangmin Gu
    Abstract:

    Background: Osteotome Sinus floor elevation is a less invasive approach to augment an insufficient alveolar bone at the posterior maxilla for dental implantation. However, this approach has some limitations due to the lack of Sinus lift tools available for clinical use and the small transcrestal access to the Maxillary Sinus floor. We recently invented shape-memory Ni/Ti alloy wire containing tube elevators for transcrestal detaching Maxillary Sinus Mucosa, and developed goat ex vivo models for direct visualizing the effectiveness of detaching Sinus Mucosa in real time during transcrestal Maxillary Sinus floor elevation. Methods: We evaluated our invented elevators, namely elevator 012 and elevator 014, for their effectiveness for transcrestal detaching Maxillary Sinus Mucosa using the goat ex vivo models. We measured the length of Sinus Mucosa detached in mesial and distal directions or buccal and palatal directions, and the space volume created by detaching Maxillary Sinus Mucosa in mesial, distal, buccal and palatal directions using the invented elevators. Results: Elevator 012 had a shape-memory Ni/Ti alloy wire with a diameter of 0.012 inch, while elevator 014 had its shape-memory Ni/Ti alloy wire with a diameter of 0.014 inch. Elevator 012 could detach the goat Maxillary Sinus Mucosa in the mesial or distal direction for 12.1±4.3 mm, while in the buccal or palatal direction for 12.5±6.7 mm. The elevator 014 could detach the goat Maxillary Sinus Mucosa for 23.0±4.9 mm in the mesial or distal direction, and for 19.0±8.1 mm in the buccal or palatal direction. An average space volume of 1.7936±0.2079 ml was created after detaching the goat maxillay Sinus Mucosa in both mesial/distal direction and buccal/palatal direction using elevator 012; while the average space volume created using elevator 014 was 1.8764±0.2366 ml. Conclusion: Both two newly invented tube elevators could effectively detach the Maxillary Sinus Mucosa on the goat ex vivo Sinus models. Moreover, elevator 014 has advantages over the elevator 012 for the capability to detach Sinus Mucosa.

Chunhao Yang - One of the best experts on this subject based on the ideXlab platform.

  • ex vivo comparative study on three Sinus lift tools for transcrestal detaching Maxillary Sinus Mucosa
    Bioengineered bugs, 2017
    Co-Authors: Yanfeng Li, Pin Hu, Xinming Dong, Chunhao Yang
    Abstract:

    ABSTRACTThe objective of this study was to comparatively evaluate 3 different Sinus lift tools, namely umbrella-shaped Sinus lift curette YSL-04, our recently designed probe-improved Sinus lift curettes, and our newly invented elevator 014, using our previous developed goat ex vivo models for direct visualizing the effectiveness of detaching Sinus Mucosa in real time. Goat ex vivo models for direct visualizing the effectiveness of detaching Sinus Mucosa in real time were generated according to our previously developed protocol. The effectiveness for each tool was evaluated through the length of Sinus Mucosa detached in mesial and distal directions or buccal and palatal directions, and the space volume created by detaching Maxillary Sinus Mucosa in mesial, distal, buccal and palatal directions. The results showed that all 3 Sinus lift tools could transcrestally detach the Maxillary Sinus Mucosa and create extra space under the elevated Sinus floor on the goat ex vivo Sinus models. Moreover, our newly inven...

  • feasibility of shape memory ni ti alloy wire containing tube elevators for transcrestal detaching Maxillary Sinus Mucosa ex vivo study
    Cellular Physiology and Biochemistry, 2016
    Co-Authors: Yanfeng Li, Fuli Wang, Pin Hu, Chunhao Yang, Xiangmin Gu
    Abstract:

    Background: Osteotome Sinus floor elevation is a less invasive approach to augment an insufficient alveolar bone at the posterior maxilla for dental implantation. However, this approach has some limitations due to the lack of Sinus lift tools available for clinical use and the small transcrestal access to the Maxillary Sinus floor. We recently invented shape-memory Ni/Ti alloy wire containing tube elevators for transcrestal detaching Maxillary Sinus Mucosa, and developed goat ex vivo models for direct visualizing the effectiveness of detaching Sinus Mucosa in real time during transcrestal Maxillary Sinus floor elevation. Methods: We evaluated our invented elevators, namely elevator 012 and elevator 014, for their effectiveness for transcrestal detaching Maxillary Sinus Mucosa using the goat ex vivo models. We measured the length of Sinus Mucosa detached in mesial and distal directions or buccal and palatal directions, and the space volume created by detaching Maxillary Sinus Mucosa in mesial, distal, buccal and palatal directions using the invented elevators. Results: Elevator 012 had a shape-memory Ni/Ti alloy wire with a diameter of 0.012 inch, while elevator 014 had its shape-memory Ni/Ti alloy wire with a diameter of 0.014 inch. Elevator 012 could detach the goat Maxillary Sinus Mucosa in the mesial or distal direction for 12.1±4.3 mm, while in the buccal or palatal direction for 12.5±6.7 mm. The elevator 014 could detach the goat Maxillary Sinus Mucosa for 23.0±4.9 mm in the mesial or distal direction, and for 19.0±8.1 mm in the buccal or palatal direction. An average space volume of 1.7936±0.2079 ml was created after detaching the goat maxillay Sinus Mucosa in both mesial/distal direction and buccal/palatal direction using elevator 012; while the average space volume created using elevator 014 was 1.8764±0.2366 ml. Conclusion: Both two newly invented tube elevators could effectively detach the Maxillary Sinus Mucosa on the goat ex vivo Sinus models. Moreover, elevator 014 has advantages over the elevator 012 for the capability to detach Sinus Mucosa.