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Omer Mohamed Khalil - One of the best experts on this subject based on the ideXlab platform.

  • Detection of (MecA)Gene in methicillin resistant Staphylococcus aureus (MRSA) at Prince A / Rhman Sidery Hospital, Al-Jouf, Saudi Arabia
    2011
    Co-Authors: Meshref Awad Al-ruaily, Omer Mohamed Khalil
    Abstract:

    The study was conducted in A/ Sidery Hospital Al-Jouf province, Saudi Arabia during, the period September 2008 to May 2009. A total of 930 patients attended the Hospital for routine investigations were screened for urinary tract infection. A total of nine hundred and thirty urine specimens were received in the laboratory for culture, 640 specimens showed significant growth from which 100 Staphylococcus aureus species were recovered. Disk diffusion method and BD phoenix TM system antibiotic susceptibility were used, 15 methicillin resistant S. aureus isolates were identified, polymerase chain reaction (PCR) was used to amplify both the S. aureus specific sequence Gene and MecA Gene with the amplicon size of 107 and 532 bp. All the isolates (n = 100) expressed S. aureus specific sequence Gene in their PCR products. The results of the PCR revealed 13/15 isolates demonstrating both resistance to methicillin and expression of MecA Gene, while the remaining two showed the resistance to methicillin by the disc diffusion method without the expression of MecA Gene. All the isolates (n=100) were sensitive to vancomycin. Key words: MecA, MRSA, methicillin resistance polymerase chain reaction (PCR),Staphylococcus aureus, Al-Jouf, Saudi Arabia.

  • detection of MecA Gene in methicillin resistant staphylococcus aureus mrsa at prince a rhman sidery hospital al jouf saudi arabia
    Journal of Medical Genetics and Genomics, 2011
    Co-Authors: Meshref Awad Alruaily, Omer Mohamed Khalil
    Abstract:

    The study was conducted in A/ Sidery Hospital Al-Jouf province, Saudi Arabia during, the period September 2008 to May 2009. A total of 930 patients attended the Hospital for routine investigations were screened for urinary tract infection. A total of nine hundred and thirty urine specimens were received in the laboratory for culture, 640 specimens showed significant growth from which 100 Staphylococcus aureus species were recovered. Disk diffusion method and BD phoenix TM system antibiotic susceptibility were used, 15 methicillin resistant S. aureus isolates were identified, polymerase chain reaction (PCR) was used to amplify both the S. aureus specific sequence Gene and MecA Gene with the amplicon size of 107 and 532 bp. All the isolates (n = 100) expressed S. aureus specific sequence Gene in their PCR products. The results of the PCR revealed 13/15 isolates demonstrating both resistance to methicillin and expression of MecA Gene, while the remaining two showed the resistance to methicillin by the disc diffusion method without the expression of MecA Gene. All the isolates (n=100) were sensitive to vancomycin. Key words: MecA, MRSA, methicillin resistance polymerase chain reaction (PCR),Staphylococcus aureus, Al-Jouf, Saudi Arabia.

Meshref Awad Alruaily - One of the best experts on this subject based on the ideXlab platform.

  • detection of MecA Gene in methicillin resistant staphylococcus aureus mrsa at prince a rhman sidery hospital al jouf saudi arabia
    Journal of Medical Genetics and Genomics, 2011
    Co-Authors: Meshref Awad Alruaily, Omer Mohamed Khalil
    Abstract:

    The study was conducted in A/ Sidery Hospital Al-Jouf province, Saudi Arabia during, the period September 2008 to May 2009. A total of 930 patients attended the Hospital for routine investigations were screened for urinary tract infection. A total of nine hundred and thirty urine specimens were received in the laboratory for culture, 640 specimens showed significant growth from which 100 Staphylococcus aureus species were recovered. Disk diffusion method and BD phoenix TM system antibiotic susceptibility were used, 15 methicillin resistant S. aureus isolates were identified, polymerase chain reaction (PCR) was used to amplify both the S. aureus specific sequence Gene and MecA Gene with the amplicon size of 107 and 532 bp. All the isolates (n = 100) expressed S. aureus specific sequence Gene in their PCR products. The results of the PCR revealed 13/15 isolates demonstrating both resistance to methicillin and expression of MecA Gene, while the remaining two showed the resistance to methicillin by the disc diffusion method without the expression of MecA Gene. All the isolates (n=100) were sensitive to vancomycin. Key words: MecA, MRSA, methicillin resistance polymerase chain reaction (PCR),Staphylococcus aureus, Al-Jouf, Saudi Arabia.

Robert D Jenison - One of the best experts on this subject based on the ideXlab platform.

  • staph id r a rapid method for determining staphylococcus species identity and detecting the MecA Gene directly from positive blood culture
    Journal of Clinical Microbiology, 2012
    Co-Authors: Christopher Pasko, Brian Hicke, John S Dunn, Heidi Jaeckel, Dan Nieuwlandt, Diane Weed, Evelyn Woodruff, Xiaotian Zheng, Robert D Jenison
    Abstract:

    Rapid diagnosis of staphylococcal bacteremia directs appropriate antimicrobial therapy, leading to improved patient outcome. We describe herein a rapid test (<75 min) that can identify the major pathogenic strains of Staphylococcus to the species level as well as the presence or absence of the methicillin resistance determinant Gene, MecA. The test, Staph ID/R, combines a rapid isothermal nucleic acid amplification method, helicase-dependent amplification (HDA), with a chip-based array that produces unambiguous visible results. The analytic sensitivity was 1 CFU per reaction for the MecA Gene and was 1 to 250 CFU per reaction depending on the staphylococcal species present in the positive blood culture. Staph ID/R has excellent specificity as well, with no cross-reactivity observed. We validated the performance of Staph ID/R by testing 104 frozen clinical positive blood cultures and comparing the results with rpoB Gene or 16S rRNA Gene sequencing for species identity determinations and MecA Gene PCR to confirm MecA Gene results. Staph ID/R agreed with MecA Gene PCR for all samples and agreed with rpoB/16S rRNA Gene sequencing in all cases except for one sample that contained a mixture of two staphylococcal species, one of which Staph ID/R correctly identified, for an overall agreement of 99.0% (P < 0.01). Staph ID/R could potentially be used to positively affect patient management for Staphylococcus-mediated bacteremia.

Christopher Pasko - One of the best experts on this subject based on the ideXlab platform.

  • staph id r a rapid method for determining staphylococcus species identity and detecting the MecA Gene directly from positive blood culture
    Journal of Clinical Microbiology, 2012
    Co-Authors: Christopher Pasko, Brian Hicke, John S Dunn, Heidi Jaeckel, Dan Nieuwlandt, Diane Weed, Evelyn Woodruff, Xiaotian Zheng, Robert D Jenison
    Abstract:

    Rapid diagnosis of staphylococcal bacteremia directs appropriate antimicrobial therapy, leading to improved patient outcome. We describe herein a rapid test (<75 min) that can identify the major pathogenic strains of Staphylococcus to the species level as well as the presence or absence of the methicillin resistance determinant Gene, MecA. The test, Staph ID/R, combines a rapid isothermal nucleic acid amplification method, helicase-dependent amplification (HDA), with a chip-based array that produces unambiguous visible results. The analytic sensitivity was 1 CFU per reaction for the MecA Gene and was 1 to 250 CFU per reaction depending on the staphylococcal species present in the positive blood culture. Staph ID/R has excellent specificity as well, with no cross-reactivity observed. We validated the performance of Staph ID/R by testing 104 frozen clinical positive blood cultures and comparing the results with rpoB Gene or 16S rRNA Gene sequencing for species identity determinations and MecA Gene PCR to confirm MecA Gene results. Staph ID/R agreed with MecA Gene PCR for all samples and agreed with rpoB/16S rRNA Gene sequencing in all cases except for one sample that contained a mixture of two staphylococcal species, one of which Staph ID/R correctly identified, for an overall agreement of 99.0% (P < 0.01). Staph ID/R could potentially be used to positively affect patient management for Staphylococcus-mediated bacteremia.

Robert Lannigan - One of the best experts on this subject based on the ideXlab platform.

  • correlation of oxacillin mic with MecA Gene carriage in coagulase negative staphylococci
    Journal of Clinical Microbiology, 2000
    Co-Authors: Zafar Hussain, L Stoakes, Viki Massey, Deb Diagre, Viivi Fitzgerald, Sameer El Sayed, Robert Lannigan
    Abstract:

    The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from ≥4 mg/liter to ≥0.5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacillin breakpoint and the presence of the MecA Gene, 493 CoNS of 11 species were tested. The presence of the MecA Gene was determined by PCR, and oxacillin susceptibility was determined by the agar dilution method with Mueller-Hinton agar containing 2% NaCl and oxacillin (0.125 to 4.0 mg/liter). The new breakpoint correctly classified all CoNS strains with MecA as methicillin resistant and strains of Staphylococcus epidermidis , S. haemolyticus , and S. hominis without MecA as methicillin susceptible. The breakpoint of ≥0.5 mg/liter was not specific for S. cohnii , S. lugdunensis , S. saprophyticus , S. warneri , and S. xylosus , in that it categorized 70 of 74 strains of these species without MecA (94.6%) as methicillin resistant. The results of this study indicate that the new oxacillin breakpoint accurately identifies strains of CoNS with MecA but is not specific for strains of certain species of CoNS without MecA .