The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
Mamie Mizusaki Iyomasa - One of the best experts on this subject based on the ideXlab platform.
-
Beneficial effects of benzodiazepine on masticatory Muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study.
Scientific Reports, 2020Co-Authors: Glauce Crivelaro Do Nascimento, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Iisei Watanabe, Bruno Lima Malzone, Christie Ramos Andrade Leite-panissi, Ramón FuentesAbstract:Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the Medial Pterygoid Muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and Medial Pterygoid Muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral Muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in Muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the Medial Pterygoid Muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory Muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.
-
metabolic and vascular pattern in Medial Pterygoid Muscle is altered by chronic stress in an animal model of hypodontia
Physiology & Behavior, 2018Co-Authors: Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Ricardo Alexandre Junqueira Calzzani, Christie Ramos Andrade Leitepanissi, Mamie Mizusaki Iyomasa, Glauce Crivelaro Do NascimentoAbstract:Abstract Psychological stress is an important perpetuating, worsening and risk factor for temporomandibular disorders of muscular or articular origin. Occlusion instability, by the way, is considered a risk factor of this pathology and can be reproduced in some experimental animal models. The exact physiologic mechanism underlying these relations however, remains unclear. Our purpose was to test the hypothesis that chronic stress and unilateral exodontia induce metabolic and vascular changes in the Medial Pterygoid Muscle of rats. Adult Wistar rats were submitted to chronic unpredictable stress and/or unilateral exodontia and their plasma and Medial Pterygoid Muscle were removed for analysis. The parameters evaluated included plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, oxidative capacity by nicotinamide adenine dinucleotide diaphorase, capillary density by laminin and alfa-CD staining and reactive oxidative species production. Chronic unpredictable stress as an isolated factor, increased oxidative metabolism, capillary density and reactive oxygen species production at Medial Pterygoid Muscle. Conversely, exodontia has a main effect in metabolism, promoting glycolytic transformation of Muscle fibers. Association of both factors induced a major glycolytic pattern in Muscle and vascular changes. Our findings provide insights into the mechanisms, possibly inducing metabolic and vascular alterations on Medial Pterygoid Muscle of rats, by which chronic stress and occlusal instabilities might be involved as risk factors in the pathophysiology of temporomandibular disorders with muscular components.
-
chronic stress effects in contralateral Medial Pterygoid Muscle of rats with occlusion alteration
Physiology & Behavior, 2016Co-Authors: Bruno Melo Loyola, Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Christie Ramos Andrade Leitepanissi, Glauce Crivelaro Do Nascimento, Joao Paulo Mardegan Issa, Mamie Mizusaki IyomasaAbstract:Abstract Temporomandibular disorder (TMD) has a high prevalence in our society, characterized by a severe pain condition of the masticatory Muscles and temporomandibular joint. Despite the indication of multiple factor initiators of TMD, there is still controversy about its etiology and its pathophysiology is poorly understood. Using rats as experimental animals we investigated the effect of unpredictable chronic stress with or without unilateral molar extraction on the contralateral Medial Pterygoid Muscle. Our hypothesis is that these two factors induce changes in morphology, oxidative metabolism and oxidative stress of Muscle fibers. Young adult male Wistar rats (± 200 g) were divided into four groups: a group with extraction and unpredictable chronic stress (E + US); with extraction and without stress (E + C); without extraction and with unpredictable chronic stress (NO + US); and a control group without either extraction or stress (NO + C). The animals were subjected to unilateral extraction of the upper left molars, under intraperitoneal anesthesia with 4% Xylazine (10 mg/kg) and 10% Ketamine (80 mg/kg) on day zero. The rats of groups E + US and NO + US were submitted to different protocols of stress, from the 14th day after the extraction. The protocols were different every day for five consecutive days, which were repeated from the 6th day for five days more. Contralateral Medial Pterygoid Muscles were obtained on the 24th day after the start of the experiment for morphological, metabolic, capillary density, and oxidative stress analysis. The data from capillary density showed a decrease of capillaries in animals subjected to dental extraction, compared with those without extraction and an increase of laminin expression in the group submitted to the unpredictable chronic stress when compared to the unexposed to stress. SDH test revealed a decrease of light fibers in the group submitted to unilateral extraction of molars, compared with this area in the control group. In E + US and NO + US groups, the deeply stained fibers increased compared to NO + C.·The exodontia factor was able to increase the ROS activity in Muscle, whereas the stress factor does not significantly alter ROS in this tissue. It was concluded that both unpredictable chronic stress and the extraction induce metabolic and density of capillary changes in the contralateral Medial Pterygoid Muscle to extraction, suggesting that these factors for a longer period of this experiment could induce Muscle damage related to TMD.
-
ultrastructure of the myotendinous junction of the Medial Pterygoid Muscle of adult and aged wistar rats
Micron, 2010Co-Authors: Adriano Polican Ciena, Mamie Mizusaki Iyomasa, Isabela Ugo Luques, Fernando Jose Dias, Sonia Regina Yokomizo De Almeida, Iisei WatanabeAbstract:The myotendon junction (MTJ) is a specialised area into the Muscle fibers where the sarcoplasmic membranes connect to the collagen fibers bundles. There are few data about plasticity of the MTJ in aging processes. The aim of this study is to analyse the ultrastructure characteristics of MTJ of Medial Pterygoid Muscle of adult and aged rats. Employing the transmission electron microscopy method, twenty male rats Wistar (Rattus norvegicus) were divided into two groups: A (n = 10) with 12 months of age; B (n = 10) 24 months of age. The animals were anaesthetised with overdose the urethane (3 g/kg, i.p.) and sacrified during the perfusion with modified Karnovsky solution. The specimens were post-fixed in a 1% osmium tetroxide solution, dehydrated in ascending concentration of ethanol and embedded in Spurr resin. The thin sections, of 90 nm thick, were counterstained with uranyl acetate and lead citrate solution, and examined in a Jeol 1010 transmission electron microscope. The fine structure of the MTJ of group A revealed the defined interdigitations and disposed in several levels of deep formations containing the collagen fibers. In the group B, such structures did not observed, detecting the projections irregular in shape, and large of extra matrix with in aspect of remodelling. In conclusion it was possible to identify the plasticity of MTJ in the group B which presented several morphological alterations comparing to the adult animals. These data of group B suggested the occurrence of aging processes in the MTJ in rats.
-
ultrastructural characteristics of the gerbil Pterygoid Medial Muscle after experimental occlusal alteration
Micron, 2009Co-Authors: Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Maria Bernadete Sasso Stuani, Ana Maria De Oliveira, Marcia Consentino Kronka Sosthines, Aracy Akiko Motoyama, Iisei WatanabeAbstract:Ten male young adult gerbils Meriones unguiculatus weighing 55 g were divided in two groups: experimental (n = 5) animals submitted to exodontia of the left upper molars, and sham-operated were used as control (n = 5) group. The aim of the present study was to investigate the ultrastructural effects of occlusal alteration induced by unilateral exodontia on Medial Pterygoid Muscle. After 60 days, the animals were sacrificed by perfusion intracardially with a modified Karnovsky solution after anesthetized with overdose of urethane (3 g/kg i.p.). The small samples of this Muscle were fixed in 2.5% glutaraldehyde solution and post-fixed in 1% buffered osmium tetroxide solution, dehydrated in ascending concentration of ethanol, and embedded in Spurr resin. Several micrographs data showed that in this period of time, the unilateral teeth extraction was able to induce modifications on the Medial Pterygoid Muscle fibers and capillaries at ultrastructural levels as compared to control Muscles and to the ones in the contralateral side of the experimental animals. Ultrastructural changes suggest that injury was greater in the hypofunctional Muscle, ipsilateral to teeth extraction.
Iisei Watanabe - One of the best experts on this subject based on the ideXlab platform.
-
Beneficial effects of benzodiazepine on masticatory Muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study.
Scientific Reports, 2020Co-Authors: Glauce Crivelaro Do Nascimento, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Iisei Watanabe, Bruno Lima Malzone, Christie Ramos Andrade Leite-panissi, Ramón FuentesAbstract:Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the Medial Pterygoid Muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and Medial Pterygoid Muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral Muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in Muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the Medial Pterygoid Muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory Muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.
-
ultrastructure of the myotendinous junction of the Medial Pterygoid Muscle of adult and aged wistar rats
Micron, 2010Co-Authors: Adriano Polican Ciena, Mamie Mizusaki Iyomasa, Isabela Ugo Luques, Fernando Jose Dias, Sonia Regina Yokomizo De Almeida, Iisei WatanabeAbstract:The myotendon junction (MTJ) is a specialised area into the Muscle fibers where the sarcoplasmic membranes connect to the collagen fibers bundles. There are few data about plasticity of the MTJ in aging processes. The aim of this study is to analyse the ultrastructure characteristics of MTJ of Medial Pterygoid Muscle of adult and aged rats. Employing the transmission electron microscopy method, twenty male rats Wistar (Rattus norvegicus) were divided into two groups: A (n = 10) with 12 months of age; B (n = 10) 24 months of age. The animals were anaesthetised with overdose the urethane (3 g/kg, i.p.) and sacrified during the perfusion with modified Karnovsky solution. The specimens were post-fixed in a 1% osmium tetroxide solution, dehydrated in ascending concentration of ethanol and embedded in Spurr resin. The thin sections, of 90 nm thick, were counterstained with uranyl acetate and lead citrate solution, and examined in a Jeol 1010 transmission electron microscope. The fine structure of the MTJ of group A revealed the defined interdigitations and disposed in several levels of deep formations containing the collagen fibers. In the group B, such structures did not observed, detecting the projections irregular in shape, and large of extra matrix with in aspect of remodelling. In conclusion it was possible to identify the plasticity of MTJ in the group B which presented several morphological alterations comparing to the adult animals. These data of group B suggested the occurrence of aging processes in the MTJ in rats.
-
ultrastructural characteristics of the gerbil Pterygoid Medial Muscle after experimental occlusal alteration
Micron, 2009Co-Authors: Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Maria Bernadete Sasso Stuani, Ana Maria De Oliveira, Marcia Consentino Kronka Sosthines, Aracy Akiko Motoyama, Iisei WatanabeAbstract:Ten male young adult gerbils Meriones unguiculatus weighing 55 g were divided in two groups: experimental (n = 5) animals submitted to exodontia of the left upper molars, and sham-operated were used as control (n = 5) group. The aim of the present study was to investigate the ultrastructural effects of occlusal alteration induced by unilateral exodontia on Medial Pterygoid Muscle. After 60 days, the animals were sacrificed by perfusion intracardially with a modified Karnovsky solution after anesthetized with overdose of urethane (3 g/kg i.p.). The small samples of this Muscle were fixed in 2.5% glutaraldehyde solution and post-fixed in 1% buffered osmium tetroxide solution, dehydrated in ascending concentration of ethanol, and embedded in Spurr resin. Several micrographs data showed that in this period of time, the unilateral teeth extraction was able to induce modifications on the Medial Pterygoid Muscle fibers and capillaries at ultrastructural levels as compared to control Muscles and to the ones in the contralateral side of the experimental animals. Ultrastructural changes suggest that injury was greater in the hypofunctional Muscle, ipsilateral to teeth extraction.
-
morphological and histological effects on the Medial Pterygoid Muscle after unilateral exodontia in gerbils
Micron, 2008Co-Authors: Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Fernando De Oliveira, Maria Bernadete Sasso Stuani, Ana Maria De Oliveira, Iisei WatanabeAbstract:Abstract Morphological effects on the Medial Pterygoid Muscle were evaluated in 20 male gerbils (average weight, 55 g) after occlusal alterations induced by extraction of left side molar teeth. Controls were only submitted to surgical stress. Sixty days after surgery, the groups were divided into two subgroups for the following studies: (1) observation of macroscopic morphology and vessels distribution (n = 10); (2) light microscopy histological analysis (n = 10). Group results were statistically compared using the Wilcoxon and Mann–Whitney tests, with a significant value of p
-
ultrastructural and biochemical changes of the Medial Pterygoid Muscle induced by unilateral exodontia
Micron, 2008Co-Authors: Emmanuel Bazan, Iisei Watanabe, Joao Paulo Mardegan Issa, Carlos Alberto Mandarimdelacerda, Mamie Mizusaki IyomasaAbstract:Abstract The aim of the present study was to investigate the histological, biochemical and ultrastructural effects of occlusal alteration induced by unilateral exodontia on Medial Pterygoid Muscle in guinea pigs, Cavia porcellus. Thirty (n = 30) male guinea pigs (450 g) were divided into two groups: experimental-animals submitted to exodontia of the left upper molars, and sham-operated were used as control. The duration of the experimental period was 60 days. Medial Pterygoid Muscles from ipsilateral and contralateral side were analyzed by histological (n = 10), histochemical (n = 10), and ultrastructural (n = 10) methods. The data were submitted to statistical analysis. When the ipsilateral side was compared to the control group, it showed a significantly shorter neuromuscular spindle length (P 0.05). In the contralateral side, the neuromuscular spindles showed significantly shorter length (P
Joao Paulo Mardegan Issa - One of the best experts on this subject based on the ideXlab platform.
-
Beneficial effects of benzodiazepine on masticatory Muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study.
Scientific Reports, 2020Co-Authors: Glauce Crivelaro Do Nascimento, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Iisei Watanabe, Bruno Lima Malzone, Christie Ramos Andrade Leite-panissi, Ramón FuentesAbstract:Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the Medial Pterygoid Muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and Medial Pterygoid Muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral Muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in Muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the Medial Pterygoid Muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory Muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.
-
chronic stress effects in contralateral Medial Pterygoid Muscle of rats with occlusion alteration
Physiology & Behavior, 2016Co-Authors: Bruno Melo Loyola, Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Christie Ramos Andrade Leitepanissi, Glauce Crivelaro Do Nascimento, Joao Paulo Mardegan Issa, Mamie Mizusaki IyomasaAbstract:Abstract Temporomandibular disorder (TMD) has a high prevalence in our society, characterized by a severe pain condition of the masticatory Muscles and temporomandibular joint. Despite the indication of multiple factor initiators of TMD, there is still controversy about its etiology and its pathophysiology is poorly understood. Using rats as experimental animals we investigated the effect of unpredictable chronic stress with or without unilateral molar extraction on the contralateral Medial Pterygoid Muscle. Our hypothesis is that these two factors induce changes in morphology, oxidative metabolism and oxidative stress of Muscle fibers. Young adult male Wistar rats (± 200 g) were divided into four groups: a group with extraction and unpredictable chronic stress (E + US); with extraction and without stress (E + C); without extraction and with unpredictable chronic stress (NO + US); and a control group without either extraction or stress (NO + C). The animals were subjected to unilateral extraction of the upper left molars, under intraperitoneal anesthesia with 4% Xylazine (10 mg/kg) and 10% Ketamine (80 mg/kg) on day zero. The rats of groups E + US and NO + US were submitted to different protocols of stress, from the 14th day after the extraction. The protocols were different every day for five consecutive days, which were repeated from the 6th day for five days more. Contralateral Medial Pterygoid Muscles were obtained on the 24th day after the start of the experiment for morphological, metabolic, capillary density, and oxidative stress analysis. The data from capillary density showed a decrease of capillaries in animals subjected to dental extraction, compared with those without extraction and an increase of laminin expression in the group submitted to the unpredictable chronic stress when compared to the unexposed to stress. SDH test revealed a decrease of light fibers in the group submitted to unilateral extraction of molars, compared with this area in the control group. In E + US and NO + US groups, the deeply stained fibers increased compared to NO + C.·The exodontia factor was able to increase the ROS activity in Muscle, whereas the stress factor does not significantly alter ROS in this tissue. It was concluded that both unpredictable chronic stress and the extraction induce metabolic and density of capillary changes in the contralateral Medial Pterygoid Muscle to extraction, suggesting that these factors for a longer period of this experiment could induce Muscle damage related to TMD.
-
ultrastructural characteristics of the gerbil Pterygoid Medial Muscle after experimental occlusal alteration
Micron, 2009Co-Authors: Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Maria Bernadete Sasso Stuani, Ana Maria De Oliveira, Marcia Consentino Kronka Sosthines, Aracy Akiko Motoyama, Iisei WatanabeAbstract:Ten male young adult gerbils Meriones unguiculatus weighing 55 g were divided in two groups: experimental (n = 5) animals submitted to exodontia of the left upper molars, and sham-operated were used as control (n = 5) group. The aim of the present study was to investigate the ultrastructural effects of occlusal alteration induced by unilateral exodontia on Medial Pterygoid Muscle. After 60 days, the animals were sacrificed by perfusion intracardially with a modified Karnovsky solution after anesthetized with overdose of urethane (3 g/kg i.p.). The small samples of this Muscle were fixed in 2.5% glutaraldehyde solution and post-fixed in 1% buffered osmium tetroxide solution, dehydrated in ascending concentration of ethanol, and embedded in Spurr resin. Several micrographs data showed that in this period of time, the unilateral teeth extraction was able to induce modifications on the Medial Pterygoid Muscle fibers and capillaries at ultrastructural levels as compared to control Muscles and to the ones in the contralateral side of the experimental animals. Ultrastructural changes suggest that injury was greater in the hypofunctional Muscle, ipsilateral to teeth extraction.
-
morphological and histological effects on the Medial Pterygoid Muscle after unilateral exodontia in gerbils
Micron, 2008Co-Authors: Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Fernando De Oliveira, Maria Bernadete Sasso Stuani, Ana Maria De Oliveira, Iisei WatanabeAbstract:Abstract Morphological effects on the Medial Pterygoid Muscle were evaluated in 20 male gerbils (average weight, 55 g) after occlusal alterations induced by extraction of left side molar teeth. Controls were only submitted to surgical stress. Sixty days after surgery, the groups were divided into two subgroups for the following studies: (1) observation of macroscopic morphology and vessels distribution (n = 10); (2) light microscopy histological analysis (n = 10). Group results were statistically compared using the Wilcoxon and Mann–Whitney tests, with a significant value of p
-
ultrastructural and biochemical changes of the Medial Pterygoid Muscle induced by unilateral exodontia
Micron, 2008Co-Authors: Emmanuel Bazan, Iisei Watanabe, Joao Paulo Mardegan Issa, Carlos Alberto Mandarimdelacerda, Mamie Mizusaki IyomasaAbstract:Abstract The aim of the present study was to investigate the histological, biochemical and ultrastructural effects of occlusal alteration induced by unilateral exodontia on Medial Pterygoid Muscle in guinea pigs, Cavia porcellus. Thirty (n = 30) male guinea pigs (450 g) were divided into two groups: experimental-animals submitted to exodontia of the left upper molars, and sham-operated were used as control. The duration of the experimental period was 60 days. Medial Pterygoid Muscles from ipsilateral and contralateral side were analyzed by histological (n = 10), histochemical (n = 10), and ultrastructural (n = 10) methods. The data were submitted to statistical analysis. When the ipsilateral side was compared to the control group, it showed a significantly shorter neuromuscular spindle length (P 0.05). In the contralateral side, the neuromuscular spindles showed significantly shorter length (P
Glauce Crivelaro Do Nascimento - One of the best experts on this subject based on the ideXlab platform.
-
Beneficial effects of benzodiazepine on masticatory Muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study.
Scientific Reports, 2020Co-Authors: Glauce Crivelaro Do Nascimento, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Iisei Watanabe, Bruno Lima Malzone, Christie Ramos Andrade Leite-panissi, Ramón FuentesAbstract:Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the Medial Pterygoid Muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and Medial Pterygoid Muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral Muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in Muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the Medial Pterygoid Muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory Muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.
-
metabolic and vascular pattern in Medial Pterygoid Muscle is altered by chronic stress in an animal model of hypodontia
Physiology & Behavior, 2018Co-Authors: Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Ricardo Alexandre Junqueira Calzzani, Christie Ramos Andrade Leitepanissi, Mamie Mizusaki Iyomasa, Glauce Crivelaro Do NascimentoAbstract:Abstract Psychological stress is an important perpetuating, worsening and risk factor for temporomandibular disorders of muscular or articular origin. Occlusion instability, by the way, is considered a risk factor of this pathology and can be reproduced in some experimental animal models. The exact physiologic mechanism underlying these relations however, remains unclear. Our purpose was to test the hypothesis that chronic stress and unilateral exodontia induce metabolic and vascular changes in the Medial Pterygoid Muscle of rats. Adult Wistar rats were submitted to chronic unpredictable stress and/or unilateral exodontia and their plasma and Medial Pterygoid Muscle were removed for analysis. The parameters evaluated included plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, oxidative capacity by nicotinamide adenine dinucleotide diaphorase, capillary density by laminin and alfa-CD staining and reactive oxidative species production. Chronic unpredictable stress as an isolated factor, increased oxidative metabolism, capillary density and reactive oxygen species production at Medial Pterygoid Muscle. Conversely, exodontia has a main effect in metabolism, promoting glycolytic transformation of Muscle fibers. Association of both factors induced a major glycolytic pattern in Muscle and vascular changes. Our findings provide insights into the mechanisms, possibly inducing metabolic and vascular alterations on Medial Pterygoid Muscle of rats, by which chronic stress and occlusal instabilities might be involved as risk factors in the pathophysiology of temporomandibular disorders with muscular components.
-
chronic stress effects in contralateral Medial Pterygoid Muscle of rats with occlusion alteration
Physiology & Behavior, 2016Co-Authors: Bruno Melo Loyola, Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Christie Ramos Andrade Leitepanissi, Glauce Crivelaro Do Nascimento, Joao Paulo Mardegan Issa, Mamie Mizusaki IyomasaAbstract:Abstract Temporomandibular disorder (TMD) has a high prevalence in our society, characterized by a severe pain condition of the masticatory Muscles and temporomandibular joint. Despite the indication of multiple factor initiators of TMD, there is still controversy about its etiology and its pathophysiology is poorly understood. Using rats as experimental animals we investigated the effect of unpredictable chronic stress with or without unilateral molar extraction on the contralateral Medial Pterygoid Muscle. Our hypothesis is that these two factors induce changes in morphology, oxidative metabolism and oxidative stress of Muscle fibers. Young adult male Wistar rats (± 200 g) were divided into four groups: a group with extraction and unpredictable chronic stress (E + US); with extraction and without stress (E + C); without extraction and with unpredictable chronic stress (NO + US); and a control group without either extraction or stress (NO + C). The animals were subjected to unilateral extraction of the upper left molars, under intraperitoneal anesthesia with 4% Xylazine (10 mg/kg) and 10% Ketamine (80 mg/kg) on day zero. The rats of groups E + US and NO + US were submitted to different protocols of stress, from the 14th day after the extraction. The protocols were different every day for five consecutive days, which were repeated from the 6th day for five days more. Contralateral Medial Pterygoid Muscles were obtained on the 24th day after the start of the experiment for morphological, metabolic, capillary density, and oxidative stress analysis. The data from capillary density showed a decrease of capillaries in animals subjected to dental extraction, compared with those without extraction and an increase of laminin expression in the group submitted to the unpredictable chronic stress when compared to the unexposed to stress. SDH test revealed a decrease of light fibers in the group submitted to unilateral extraction of molars, compared with this area in the control group. In E + US and NO + US groups, the deeply stained fibers increased compared to NO + C.·The exodontia factor was able to increase the ROS activity in Muscle, whereas the stress factor does not significantly alter ROS in this tissue. It was concluded that both unpredictable chronic stress and the extraction induce metabolic and density of capillary changes in the contralateral Medial Pterygoid Muscle to extraction, suggesting that these factors for a longer period of this experiment could induce Muscle damage related to TMD.
Daniela Mizusaki Iyomasa - One of the best experts on this subject based on the ideXlab platform.
-
Beneficial effects of benzodiazepine on masticatory Muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study.
Scientific Reports, 2020Co-Authors: Glauce Crivelaro Do Nascimento, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Mamie Mizusaki Iyomasa, Joao Paulo Mardegan Issa, Iisei Watanabe, Bruno Lima Malzone, Christie Ramos Andrade Leite-panissi, Ramón FuentesAbstract:Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the Medial Pterygoid Muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and Medial Pterygoid Muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral Muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in Muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the Medial Pterygoid Muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory Muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.
-
metabolic and vascular pattern in Medial Pterygoid Muscle is altered by chronic stress in an animal model of hypodontia
Physiology & Behavior, 2018Co-Authors: Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Ricardo Alexandre Junqueira Calzzani, Christie Ramos Andrade Leitepanissi, Mamie Mizusaki Iyomasa, Glauce Crivelaro Do NascimentoAbstract:Abstract Psychological stress is an important perpetuating, worsening and risk factor for temporomandibular disorders of muscular or articular origin. Occlusion instability, by the way, is considered a risk factor of this pathology and can be reproduced in some experimental animal models. The exact physiologic mechanism underlying these relations however, remains unclear. Our purpose was to test the hypothesis that chronic stress and unilateral exodontia induce metabolic and vascular changes in the Medial Pterygoid Muscle of rats. Adult Wistar rats were submitted to chronic unpredictable stress and/or unilateral exodontia and their plasma and Medial Pterygoid Muscle were removed for analysis. The parameters evaluated included plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, oxidative capacity by nicotinamide adenine dinucleotide diaphorase, capillary density by laminin and alfa-CD staining and reactive oxidative species production. Chronic unpredictable stress as an isolated factor, increased oxidative metabolism, capillary density and reactive oxygen species production at Medial Pterygoid Muscle. Conversely, exodontia has a main effect in metabolism, promoting glycolytic transformation of Muscle fibers. Association of both factors induced a major glycolytic pattern in Muscle and vascular changes. Our findings provide insights into the mechanisms, possibly inducing metabolic and vascular alterations on Medial Pterygoid Muscle of rats, by which chronic stress and occlusal instabilities might be involved as risk factors in the pathophysiology of temporomandibular disorders with muscular components.
-
chronic stress effects in contralateral Medial Pterygoid Muscle of rats with occlusion alteration
Physiology & Behavior, 2016Co-Authors: Bruno Melo Loyola, Rodrigo Alberto Restrepo Fernandez, Yamba Carla Lara Pereira, Daniela Mizusaki Iyomasa, Christie Ramos Andrade Leitepanissi, Glauce Crivelaro Do Nascimento, Joao Paulo Mardegan Issa, Mamie Mizusaki IyomasaAbstract:Abstract Temporomandibular disorder (TMD) has a high prevalence in our society, characterized by a severe pain condition of the masticatory Muscles and temporomandibular joint. Despite the indication of multiple factor initiators of TMD, there is still controversy about its etiology and its pathophysiology is poorly understood. Using rats as experimental animals we investigated the effect of unpredictable chronic stress with or without unilateral molar extraction on the contralateral Medial Pterygoid Muscle. Our hypothesis is that these two factors induce changes in morphology, oxidative metabolism and oxidative stress of Muscle fibers. Young adult male Wistar rats (± 200 g) were divided into four groups: a group with extraction and unpredictable chronic stress (E + US); with extraction and without stress (E + C); without extraction and with unpredictable chronic stress (NO + US); and a control group without either extraction or stress (NO + C). The animals were subjected to unilateral extraction of the upper left molars, under intraperitoneal anesthesia with 4% Xylazine (10 mg/kg) and 10% Ketamine (80 mg/kg) on day zero. The rats of groups E + US and NO + US were submitted to different protocols of stress, from the 14th day after the extraction. The protocols were different every day for five consecutive days, which were repeated from the 6th day for five days more. Contralateral Medial Pterygoid Muscles were obtained on the 24th day after the start of the experiment for morphological, metabolic, capillary density, and oxidative stress analysis. The data from capillary density showed a decrease of capillaries in animals subjected to dental extraction, compared with those without extraction and an increase of laminin expression in the group submitted to the unpredictable chronic stress when compared to the unexposed to stress. SDH test revealed a decrease of light fibers in the group submitted to unilateral extraction of molars, compared with this area in the control group. In E + US and NO + US groups, the deeply stained fibers increased compared to NO + C.·The exodontia factor was able to increase the ROS activity in Muscle, whereas the stress factor does not significantly alter ROS in this tissue. It was concluded that both unpredictable chronic stress and the extraction induce metabolic and density of capillary changes in the contralateral Medial Pterygoid Muscle to extraction, suggesting that these factors for a longer period of this experiment could induce Muscle damage related to TMD.