The Experts below are selected from a list of 696 Experts worldwide ranked by ideXlab platform

A. Deva - One of the best experts on this subject based on the ideXlab platform.

  • TO ASSESS THE EFFECTIVENESS OF TOPICAL NEGATIVE PRESSURE (TNP) DRESSINGS COMBINED WITH LOCAL INSTILLATION OF BIOCIDES AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND PSEUDOMONAS AERUGINOSA BIOFILM IN VITRO
    2012
    Co-Authors: R. Mittal, K. Vickery, Q. Ngo, A. Deva
    Abstract:

    S epidermidis and P aeruginosa are recognised major biofilm pathogens in Medical Device Contamination and chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix that cements them to each other and to the surface and protects them by increasing resistance to host immunity, antibiotics and biocides. Staph and pseudomonas spp biofilm were grown on glass coupons for 48 hours and the coupons randomly inserted into the wound model for 24 hours and subjected to TNP and the following: No instillation 0.1% w/v formulated hypocholorous acid (FHA) instillation Saline instillation Betadine instillation Betadine and saline instillations were for 30 minutes, while FHA was for three minutes, every eight, four and two hours per day. The biocides were at sub-lethal concentrations. The coupons were then extracted to avoid damaging the biofilms and effect of TNP was assessed by colony forming units and electron microscopy. The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in growth of staph spp. Frequent flushing of the wound model resulted in a loss of biofilm bacteria for both Pseudomonas and Staph epi. The biocides combined with TNP were more effective in killing Pseudomonas compared with Staph epi.

  • PR52P TO ASSESS THE EFFECTIVENESS OF TOPICAL NEGATIVE PRESSURE (TNP) COMBINED WITH LOCAL INSTILLATION OF BIOCIDES AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND PSEUDOMONAS AERUGINOSA BIOFILM IN VITRO
    ANZ Journal of Surgery, 2009
    Co-Authors: R. Mittal, K. Vickery, Q. Ngo, A. Deva
    Abstract:

    Introduction:   S epidermidis is a recognised major biofilm pathogen and has been implicated in Medical Device Contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications. Method:   Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to: 1 No instillation plus TNP 2 0.1% w/v formulated hypocholorous acid instillation plus TNP 3 Saline instillation (no biocide) plus TNP 4 Betadine instillation plus TNP Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy. Results:   The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.

Howard Ceri - One of the best experts on this subject based on the ideXlab platform.

  • Synergistic effect of lipopeptide biosurfactant with antibiotics against Escherichia coli CFT073 biofilm.
    International journal of antimicrobial agents, 2011
    Co-Authors: Fabrizio Rivardo, Maria Giovanna Martinotti, Raymond J. Turner, Howard Ceri
    Abstract:

    Abstract Biofilms are microcolonies of microbes adherent to biotic and abiotic surfaces, often responsible for chronic infections and Medical Device Contamination. Escherichia coli is one of the prevalent pathogens involved in uropathogenic infections and Contamination of catheters. A biosurfactant produced by Bacillus licheniformis V9T14 was tested alone and in association with various antibiotics against a mature 24-h uropathogenic E. coli CFT073 biofilm. Biofilm was grown on polystyrene pegs of a Calgary Biofilm Device, providing a tool to evaluate the efficacy of antimicrobial agents. Antibiotics tested were ampicillin, cefazolin, ceftriaxone, ciprofloxacin, piperacillin, tobramycin and trimethoprim/sulfamethoxazole (19:1). Biosurfactant alone at the concentrations tested was not able to remove the adherent cells of the pre-formed biofilm. However, the difference between the effect of antibiotic alone and in combination with the biosurfactant was significant and exceeded 1log 10 (90%) reduction in most cases. Results of this study indicate that V9T14 biosurfactant in association with antibiotics leads to a synergistic increase in the efficacy of antibiotics in biofilm killing, and in some combinations leads to total eradication of E. coli CFT073 biofilm.

R. Mittal - One of the best experts on this subject based on the ideXlab platform.

  • TO ASSESS THE EFFECTIVENESS OF TOPICAL NEGATIVE PRESSURE (TNP) DRESSINGS COMBINED WITH LOCAL INSTILLATION OF BIOCIDES AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND PSEUDOMONAS AERUGINOSA BIOFILM IN VITRO
    2012
    Co-Authors: R. Mittal, K. Vickery, Q. Ngo, A. Deva
    Abstract:

    S epidermidis and P aeruginosa are recognised major biofilm pathogens in Medical Device Contamination and chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix that cements them to each other and to the surface and protects them by increasing resistance to host immunity, antibiotics and biocides. Staph and pseudomonas spp biofilm were grown on glass coupons for 48 hours and the coupons randomly inserted into the wound model for 24 hours and subjected to TNP and the following: No instillation 0.1% w/v formulated hypocholorous acid (FHA) instillation Saline instillation Betadine instillation Betadine and saline instillations were for 30 minutes, while FHA was for three minutes, every eight, four and two hours per day. The biocides were at sub-lethal concentrations. The coupons were then extracted to avoid damaging the biofilms and effect of TNP was assessed by colony forming units and electron microscopy. The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in growth of staph spp. Frequent flushing of the wound model resulted in a loss of biofilm bacteria for both Pseudomonas and Staph epi. The biocides combined with TNP were more effective in killing Pseudomonas compared with Staph epi.

  • PR52P TO ASSESS THE EFFECTIVENESS OF TOPICAL NEGATIVE PRESSURE (TNP) COMBINED WITH LOCAL INSTILLATION OF BIOCIDES AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND PSEUDOMONAS AERUGINOSA BIOFILM IN VITRO
    ANZ Journal of Surgery, 2009
    Co-Authors: R. Mittal, K. Vickery, Q. Ngo, A. Deva
    Abstract:

    Introduction:   S epidermidis is a recognised major biofilm pathogen and has been implicated in Medical Device Contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications. Method:   Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to: 1 No instillation plus TNP 2 0.1% w/v formulated hypocholorous acid instillation plus TNP 3 Saline instillation (no biocide) plus TNP 4 Betadine instillation plus TNP Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy. Results:   The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.

Fabrizio Rivardo - One of the best experts on this subject based on the ideXlab platform.

  • Synergistic effect of lipopeptide biosurfactant with antibiotics against Escherichia coli CFT073 biofilm.
    International journal of antimicrobial agents, 2011
    Co-Authors: Fabrizio Rivardo, Maria Giovanna Martinotti, Raymond J. Turner, Howard Ceri
    Abstract:

    Abstract Biofilms are microcolonies of microbes adherent to biotic and abiotic surfaces, often responsible for chronic infections and Medical Device Contamination. Escherichia coli is one of the prevalent pathogens involved in uropathogenic infections and Contamination of catheters. A biosurfactant produced by Bacillus licheniformis V9T14 was tested alone and in association with various antibiotics against a mature 24-h uropathogenic E. coli CFT073 biofilm. Biofilm was grown on polystyrene pegs of a Calgary Biofilm Device, providing a tool to evaluate the efficacy of antimicrobial agents. Antibiotics tested were ampicillin, cefazolin, ceftriaxone, ciprofloxacin, piperacillin, tobramycin and trimethoprim/sulfamethoxazole (19:1). Biosurfactant alone at the concentrations tested was not able to remove the adherent cells of the pre-formed biofilm. However, the difference between the effect of antibiotic alone and in combination with the biosurfactant was significant and exceeded 1log 10 (90%) reduction in most cases. Results of this study indicate that V9T14 biosurfactant in association with antibiotics leads to a synergistic increase in the efficacy of antibiotics in biofilm killing, and in some combinations leads to total eradication of E. coli CFT073 biofilm.

Q. Ngo - One of the best experts on this subject based on the ideXlab platform.

  • TO ASSESS THE EFFECTIVENESS OF TOPICAL NEGATIVE PRESSURE (TNP) DRESSINGS COMBINED WITH LOCAL INSTILLATION OF BIOCIDES AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND PSEUDOMONAS AERUGINOSA BIOFILM IN VITRO
    2012
    Co-Authors: R. Mittal, K. Vickery, Q. Ngo, A. Deva
    Abstract:

    S epidermidis and P aeruginosa are recognised major biofilm pathogens in Medical Device Contamination and chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix that cements them to each other and to the surface and protects them by increasing resistance to host immunity, antibiotics and biocides. Staph and pseudomonas spp biofilm were grown on glass coupons for 48 hours and the coupons randomly inserted into the wound model for 24 hours and subjected to TNP and the following: No instillation 0.1% w/v formulated hypocholorous acid (FHA) instillation Saline instillation Betadine instillation Betadine and saline instillations were for 30 minutes, while FHA was for three minutes, every eight, four and two hours per day. The biocides were at sub-lethal concentrations. The coupons were then extracted to avoid damaging the biofilms and effect of TNP was assessed by colony forming units and electron microscopy. The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in growth of staph spp. Frequent flushing of the wound model resulted in a loss of biofilm bacteria for both Pseudomonas and Staph epi. The biocides combined with TNP were more effective in killing Pseudomonas compared with Staph epi.

  • PR52P TO ASSESS THE EFFECTIVENESS OF TOPICAL NEGATIVE PRESSURE (TNP) COMBINED WITH LOCAL INSTILLATION OF BIOCIDES AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND PSEUDOMONAS AERUGINOSA BIOFILM IN VITRO
    ANZ Journal of Surgery, 2009
    Co-Authors: R. Mittal, K. Vickery, Q. Ngo, A. Deva
    Abstract:

    Introduction:   S epidermidis is a recognised major biofilm pathogen and has been implicated in Medical Device Contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications. Method:   Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to: 1 No instillation plus TNP 2 0.1% w/v formulated hypocholorous acid instillation plus TNP 3 Saline instillation (no biocide) plus TNP 4 Betadine instillation plus TNP Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy. Results:   The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.