The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform

Hui Chen - One of the best experts on this subject based on the ideXlab platform.

  • Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages.
    BMC plant biology, 2019
    Co-Authors: Moyang Liu, Zizhong Tang, Li Huang, Wenjun Sun, Hui Chen
    Abstract:

    Background SPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase transition, and flower and fruit development. The SPL gene family has been identified and characterized in many species but has not been well studied in tartary buckwheat, which is an important edible and Medicinal Crop.

  • Genome-wide investigation of the MADS gene family and dehulling genes in tartary buckwheat ( Fagopyrum tataricum )
    Planta, 2019
    Co-Authors: Qiankun Fu, Zhaotang Ma, Zizhong Tang, Tongliang Bu, Chenglei Li, Qi Wu, Li Huang, Hui Chen
    Abstract:

    Genome-wide identification, expression analysis and potential functional characterization of previously uncharacterized MADS family of tartary buckwheat, emphasized the importance of this gene family in plant growth and development. The MADS transcription factor is a key regulatory factor in the development of most plants. The MADS gene in plants controls all aspects of tissue and organ growth and reproduction and can be used to regulate plant seed cracking. However, there has been little research on the MADS genes of tartary buckwheat (Fagopyrum tataricum), which is an important edible and Medicinal Crop. The recently published whole genome sequence of tartary buckwheat allows us to study the tissue and expression profiles of the MADS gene in tartary buckwheat at a genome-wide level. In this study, 65 MADS genes of tartary buckwheat were identified and renamed according to the chromosomal distribution of the FtMADS genes. Here, we provide a complete overview of the gene structure, gene expression, genomic mapping, protein motif organization, and phylogenetic relationships of each member of the gene family. According to the phylogenetic relationship of MADS genes, the transcription factor family was divided into two subfamilies, the M subfamily (28 genes) and the MIKC subfamily (37 genes). The results showed that the FtMADS genes belonged to related sister pairs and the chromosomal map showed that the replication of FtMADSs was related to the replication of chromosome blocks. In different tissues and at different fruit development stages, the FtMADS genes obtained by real-time quantitative PCR (RT-qPCR) showed obvious expression patterns. A comprehensive analysis of the MADS genes in tartary buckwheat was conducted. Through systematic analysis, the potential genes that may regulate the growth and development of tartary buckwheat and the genes that may regulate the easy dehulling of tartary buckwheat fruit were screened, which laid a solid foundation for improving the quality of tartary buckwheat.

Li Huang - One of the best experts on this subject based on the ideXlab platform.

  • Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages.
    BMC plant biology, 2019
    Co-Authors: Moyang Liu, Zizhong Tang, Li Huang, Wenjun Sun, Hui Chen
    Abstract:

    Background SPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase transition, and flower and fruit development. The SPL gene family has been identified and characterized in many species but has not been well studied in tartary buckwheat, which is an important edible and Medicinal Crop.

  • Genome-wide investigation of the MADS gene family and dehulling genes in tartary buckwheat ( Fagopyrum tataricum )
    Planta, 2019
    Co-Authors: Qiankun Fu, Zhaotang Ma, Zizhong Tang, Tongliang Bu, Chenglei Li, Qi Wu, Li Huang, Hui Chen
    Abstract:

    Genome-wide identification, expression analysis and potential functional characterization of previously uncharacterized MADS family of tartary buckwheat, emphasized the importance of this gene family in plant growth and development. The MADS transcription factor is a key regulatory factor in the development of most plants. The MADS gene in plants controls all aspects of tissue and organ growth and reproduction and can be used to regulate plant seed cracking. However, there has been little research on the MADS genes of tartary buckwheat (Fagopyrum tataricum), which is an important edible and Medicinal Crop. The recently published whole genome sequence of tartary buckwheat allows us to study the tissue and expression profiles of the MADS gene in tartary buckwheat at a genome-wide level. In this study, 65 MADS genes of tartary buckwheat were identified and renamed according to the chromosomal distribution of the FtMADS genes. Here, we provide a complete overview of the gene structure, gene expression, genomic mapping, protein motif organization, and phylogenetic relationships of each member of the gene family. According to the phylogenetic relationship of MADS genes, the transcription factor family was divided into two subfamilies, the M subfamily (28 genes) and the MIKC subfamily (37 genes). The results showed that the FtMADS genes belonged to related sister pairs and the chromosomal map showed that the replication of FtMADSs was related to the replication of chromosome blocks. In different tissues and at different fruit development stages, the FtMADS genes obtained by real-time quantitative PCR (RT-qPCR) showed obvious expression patterns. A comprehensive analysis of the MADS genes in tartary buckwheat was conducted. Through systematic analysis, the potential genes that may regulate the growth and development of tartary buckwheat and the genes that may regulate the easy dehulling of tartary buckwheat fruit were screened, which laid a solid foundation for improving the quality of tartary buckwheat.

Zizhong Tang - One of the best experts on this subject based on the ideXlab platform.

  • Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages.
    BMC plant biology, 2019
    Co-Authors: Moyang Liu, Zizhong Tang, Li Huang, Wenjun Sun, Hui Chen
    Abstract:

    Background SPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase transition, and flower and fruit development. The SPL gene family has been identified and characterized in many species but has not been well studied in tartary buckwheat, which is an important edible and Medicinal Crop.

  • Genome-wide investigation of the MADS gene family and dehulling genes in tartary buckwheat ( Fagopyrum tataricum )
    Planta, 2019
    Co-Authors: Qiankun Fu, Zhaotang Ma, Zizhong Tang, Tongliang Bu, Chenglei Li, Qi Wu, Li Huang, Hui Chen
    Abstract:

    Genome-wide identification, expression analysis and potential functional characterization of previously uncharacterized MADS family of tartary buckwheat, emphasized the importance of this gene family in plant growth and development. The MADS transcription factor is a key regulatory factor in the development of most plants. The MADS gene in plants controls all aspects of tissue and organ growth and reproduction and can be used to regulate plant seed cracking. However, there has been little research on the MADS genes of tartary buckwheat (Fagopyrum tataricum), which is an important edible and Medicinal Crop. The recently published whole genome sequence of tartary buckwheat allows us to study the tissue and expression profiles of the MADS gene in tartary buckwheat at a genome-wide level. In this study, 65 MADS genes of tartary buckwheat were identified and renamed according to the chromosomal distribution of the FtMADS genes. Here, we provide a complete overview of the gene structure, gene expression, genomic mapping, protein motif organization, and phylogenetic relationships of each member of the gene family. According to the phylogenetic relationship of MADS genes, the transcription factor family was divided into two subfamilies, the M subfamily (28 genes) and the MIKC subfamily (37 genes). The results showed that the FtMADS genes belonged to related sister pairs and the chromosomal map showed that the replication of FtMADSs was related to the replication of chromosome blocks. In different tissues and at different fruit development stages, the FtMADS genes obtained by real-time quantitative PCR (RT-qPCR) showed obvious expression patterns. A comprehensive analysis of the MADS genes in tartary buckwheat was conducted. Through systematic analysis, the potential genes that may regulate the growth and development of tartary buckwheat and the genes that may regulate the easy dehulling of tartary buckwheat fruit were screened, which laid a solid foundation for improving the quality of tartary buckwheat.

Andrea Maxia - One of the best experts on this subject based on the ideXlab platform.

Hung-jen Hxiao - One of the best experts on this subject based on the ideXlab platform.

  • Rapid and sensitive authentication of Polygonum multiflorum (He-Shou-Wu) of Chinese Medicinal Crop using specific isothermal nucleic acid amplification
    Industrial Crops and Products, 2019
    Co-Authors: Meng-shiou Lee, Hung-jen Hxiao
    Abstract:

    Abstract Polygoni Multiflori Radix (PMR) is well-known valuable Medicinal Crop and has been reported to have many biological functions for medical applications. For preventing the problem of PMR adulteration in the herbal market, in this study, a DNA-based molecular method, loop-mediated isothermal amplification (LAMP), was developed for the authentication of PMR. A set of newly designed LAMP primer was developed based on the internal transcribed spacer (ITS) sequences of ribosomal DNA. The results demonstrated that amplicon of PM genomic DNA was amplified successfully using specific LAMP primers when the sample contained PM genomic DNA. By contrast, the adulterants of PMR did not exhibit DNA amplification when LAMP was performed. Compared with the traditional polymerase chain reaction (PCR), the isothermal DNA amplification by LAMP demonstrated 10-fold higher sensitivity and required half the time of PCR. The PMR samples with 3-times, 6-times repeated processing, autoclave steaming, and γ-ray irradiation were also can be authenticated by LAMP. However, the PMR sample with conventional 9-times repeated processing was not authenticated by LAMP. In conclusion, the specific DNA amplification method for the authentication of PMR presented herein was sensitive, specific, and rapid under isothermal condition. The findings are useful for application of PMR authentication on-site in the herbal market. This also demonstrates the method’s potential application for the authentication of other herbal Crops in the future.