The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Carlos Caceres - One of the best experts on this subject based on the ideXlab platform.
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Notas Científicas Diets based on soybean protein for Mediterranean Fruit Fly
2020Co-Authors: Raimundo Braga Sobrinho, Carlos Caceres, Amirul Islam, Vivat Wornoayporn, Walter EnkerlinAbstract:The objective of this work was to develop suitable and economic diets for mass rearing Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae). Diets containing sugar beet bagase, wheat bran, brewer yeast, and others with wheat bran and palletized soybean protein from Brazil were tested. Diets based on soybean protein have shown promising results regarding pupal recovery, pupal weight and adult emergence. Soybean bagase in the form of pellets with 60% of protein can be a very important substitute for other expensive sources of protein.
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comparison of classical and transgenic genetic sexing strains of Mediterranean Fruit Fly diptera tephritidae for application of the sterile insect technique
PLOS ONE, 2018Co-Authors: Jose S Meza, Kostas Bourtzis, Marc J B Vreysen, Georgios A Kyritsis, Carlos CaceresAbstract:The development of genetic sexing strains (GSSs) based on classical genetic approaches has revolutionized the application of the sterile insect technique (SIT) against the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). The global use of Mediterranean Fruit Fly GSS for SIT applications as part of area-wide integrated pest management (AW-IPM) programmes is testimony to their effectiveness. During recent years, transgenic sexing strains (TSSs) have been developed through genetic engineering techniques offering the possibility to produce male-only progeny by introducing female embryonic lethal genes and to increase the efficacy to identify released sterile males by means of the expression of fluorescent transgene markers. Here, we present a comparative analysis of two Mediterranean Fruit Fly strains: the classical GSS VIENNA 8D53-/Toliman and the transgenic FSEL#32. The strains were compared for production efficiency and quality control indices under semi mass-rearing conditions, response to sterilizing irradiation doses, male mating performance in walk-in field cages, and production cost of male-only pupae. The results showed that, the FSEL #32 TSS had a similar fecundity but a higher production of male-only pupae than the VIENNA 8D53-/Toliman GSS. For some of the quality control parameters tested, such as pupal weight and survival under starvation conditions, the FSEL #32 TSS was inferior to the VIENNA 8D53-/Toliman GSS. Both the transgenic and the classical genetic sexing strains have shown acceptable and similar mating competitiveness when compared with wild males for mating with wild females. The cost production for both strains is similar but the FSEL#32 TSS may potentially be more cost effective at higher production levels. The results are discussed in the context of incorporating the transgenic strain for SIT application.
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Routine Isotope Marking for the Mediterranean Fruit Fly (Diptera: Tephritidae)
Journal of Economic Entomology, 2009Co-Authors: Rebecca Hood-nowotny, Amirul Islam, Leo Mayr, Alan S. Robinson, Carlos CaceresAbstract:ABSTRACT A simple method of marking Mediterranean Fruit Fly Ceratitis capitata (Wiedemann) using stable isotopes is described. This species is economically important and is a target species of many successful area-wide integrated pest management (AW-IPM) programs using the sterile insect technique (SIT). Program monitoring in the field relies on being able to accurately differentiate released sterile insects from wild insects so that estimates can be made of the ratio of sterile males to wild males. Typically, released flies are marked with fluorescent dust, which is not always reliable. The difference in isotopic signatures between wild and factory-reared populations could be a reliable and intrinsic secondary marker to complement existing marking methods. Isotopic signatures are natural differences in stable isotope composition of organisms caused by discrimination against the heavier isotopes during some biological processes. The isotopic signature of an organism is mainly dependent on what it eats; by...
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Diets based on soybean protein for Mediterranean Fruit Fly
Pesquisa Agropecuaria Brasileira, 2006Co-Authors: Raimundo Braga Sobrinho, Carlos Caceres, Amirul Islam, Vivat Wornoayporn, Walter EnkerlinAbstract:The objective of this work was to develop suitable and economic diets for mass rearing Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae). Diets containing sugar beet bagase, wheat bran, brewer yeast, and others with wheat bran and palletized soybean protein from Brazil were tested. Diets based on soybean protein have shown promising results regarding pupal recovery, pupal weight and adult emergence. Soybean bagase in the form of pellets with 60% of protein can be a very important substitute for other expensive sources of protein.
Mariangela Bonizzoni - One of the best experts on this subject based on the ideXlab platform.
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Unfaithful Mediterranean Fruit Fly Ceratitis capitata Females: Impact on the SIT?
Area-Wide Control of Insect Pests, 2020Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, S. Bertin, Francesca Scolari, C. R. Guglielmino, Boaz Yuval, Giuliano Gasperi, Anna R. MalacridaAbstract:An understanding of the levels of remating and paternity skew in the field can be important for polyphagous pest species with a high colonization potential such as the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann). The use of polymorphic simple sequence repeats on flies from two Mediterranean populations in combination with various statistical methods showed not only that Mediterranean Fruit Fly females remate in the wild, but most importantly, that the level of sperm precedence could influence the effect of remating itself since one male, presumably the last, tends to sire most of the progeny. Levels of remating and paternity skew may have important implications for the evolution of the species in terms of maintenance of genetic variability. Moreover, these features of mating behaviour may locally affect the efficiency of the sterile insect technique (SIT), which is a commonly applied control strategy against the Mediterranean Fruit Fly.
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microsatellite analysis reveals remating by wild Mediterranean Fruit Fly females ceratitis capitata
Molecular Ecology, 2002Co-Authors: Mariangela Bonizzoni, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Byron I Katsoyannos, R Marguerie, Tracey ChapmanAbstract:Accurate estimates of remating in wild female insects are required for an understanding of the causes of variation in remating between individuals, populations and species. Such estimates are also of profound importance for major economic Fruit pests such as the Mediterranean Fruit Fly (Ceratitis capitata). A major method for the suppression of this pest is the sterile insect technique (SIT), which relies on matings between mass-reared, sterilized males and wild females. Remating by wild females will thus impact negatively on the success of SIT. We used microsatellite markers to determine the level of remating in wild (field-collected) Mediterranean Fruit Fly females from the Greek Island of Chios. We compared the four locus microsatellite genotypes of these females and their offspring. Our data showed 7.1% of wild females remated. Skewed paternity among progeny arrays provided further evidence for double matings. Our lowest estimate of remating was 3.8% and the highest was 21%.
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microsatellite polymorphism in the Mediterranean Fruit Fly ceratitis capitata
Insect Molecular Biology, 2000Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Liangbiao ZhengAbstract:A total of forty-three simple sequence repeats (SSRs) were identified in the Mediterranean Fruit Fly (medFly) Ceratitis capitata . The most common SSR was the dinucleotide (TG) n /(CA) n occurring in thirty of the forty-three microsatellite loci. Polymorphism at ten dinucleotide markers was investigated in 122 flies from six natural populations sampled in the native and colonized areas. A very high level of allelic diversity was detected in the species range. An average of 13.6 alleles was found over all the ten loci indicating the informativeness of SSRs as genetic markers for the medFly. The distribution of microsatellite polymorphism in the species range reflects the medFly colonization history.
Anna R. Malacrida - One of the best experts on this subject based on the ideXlab platform.
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Unfaithful Mediterranean Fruit Fly Ceratitis capitata Females: Impact on the SIT?
Area-Wide Control of Insect Pests, 2020Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, S. Bertin, Francesca Scolari, C. R. Guglielmino, Boaz Yuval, Giuliano Gasperi, Anna R. MalacridaAbstract:An understanding of the levels of remating and paternity skew in the field can be important for polyphagous pest species with a high colonization potential such as the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann). The use of polymorphic simple sequence repeats on flies from two Mediterranean populations in combination with various statistical methods showed not only that Mediterranean Fruit Fly females remate in the wild, but most importantly, that the level of sperm precedence could influence the effect of remating itself since one male, presumably the last, tends to sire most of the progeny. Levels of remating and paternity skew may have important implications for the evolution of the species in terms of maintenance of genetic variability. Moreover, these features of mating behaviour may locally affect the efficiency of the sterile insect technique (SIT), which is a commonly applied control strategy against the Mediterranean Fruit Fly.
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microsatellite analysis reveals remating by wild Mediterranean Fruit Fly females ceratitis capitata
Molecular Ecology, 2002Co-Authors: Mariangela Bonizzoni, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Byron I Katsoyannos, R Marguerie, Tracey ChapmanAbstract:Accurate estimates of remating in wild female insects are required for an understanding of the causes of variation in remating between individuals, populations and species. Such estimates are also of profound importance for major economic Fruit pests such as the Mediterranean Fruit Fly (Ceratitis capitata). A major method for the suppression of this pest is the sterile insect technique (SIT), which relies on matings between mass-reared, sterilized males and wild females. Remating by wild females will thus impact negatively on the success of SIT. We used microsatellite markers to determine the level of remating in wild (field-collected) Mediterranean Fruit Fly females from the Greek Island of Chios. We compared the four locus microsatellite genotypes of these females and their offspring. Our data showed 7.1% of wild females remated. Skewed paternity among progeny arrays provided further evidence for double matings. Our lowest estimate of remating was 3.8% and the highest was 21%.
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microsatellite polymorphism in the Mediterranean Fruit Fly ceratitis capitata
Insect Molecular Biology, 2000Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Liangbiao ZhengAbstract:A total of forty-three simple sequence repeats (SSRs) were identified in the Mediterranean Fruit Fly (medFly) Ceratitis capitata . The most common SSR was the dinucleotide (TG) n /(CA) n occurring in thirty of the forty-three microsatellite loci. Polymorphism at ten dinucleotide markers was investigated in 122 flies from six natural populations sampled in the native and colonized areas. A very high level of allelic diversity was detected in the species range. An average of 13.6 alleles was found over all the ten loci indicating the informativeness of SSRs as genetic markers for the medFly. The distribution of microsatellite polymorphism in the species range reflects the medFly colonization history.
Giuliano Gasperi - One of the best experts on this subject based on the ideXlab platform.
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Unfaithful Mediterranean Fruit Fly Ceratitis capitata Females: Impact on the SIT?
Area-Wide Control of Insect Pests, 2020Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, S. Bertin, Francesca Scolari, C. R. Guglielmino, Boaz Yuval, Giuliano Gasperi, Anna R. MalacridaAbstract:An understanding of the levels of remating and paternity skew in the field can be important for polyphagous pest species with a high colonization potential such as the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann). The use of polymorphic simple sequence repeats on flies from two Mediterranean populations in combination with various statistical methods showed not only that Mediterranean Fruit Fly females remate in the wild, but most importantly, that the level of sperm precedence could influence the effect of remating itself since one male, presumably the last, tends to sire most of the progeny. Levels of remating and paternity skew may have important implications for the evolution of the species in terms of maintenance of genetic variability. Moreover, these features of mating behaviour may locally affect the efficiency of the sterile insect technique (SIT), which is a commonly applied control strategy against the Mediterranean Fruit Fly.
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microsatellite analysis reveals remating by wild Mediterranean Fruit Fly females ceratitis capitata
Molecular Ecology, 2002Co-Authors: Mariangela Bonizzoni, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Byron I Katsoyannos, R Marguerie, Tracey ChapmanAbstract:Accurate estimates of remating in wild female insects are required for an understanding of the causes of variation in remating between individuals, populations and species. Such estimates are also of profound importance for major economic Fruit pests such as the Mediterranean Fruit Fly (Ceratitis capitata). A major method for the suppression of this pest is the sterile insect technique (SIT), which relies on matings between mass-reared, sterilized males and wild females. Remating by wild females will thus impact negatively on the success of SIT. We used microsatellite markers to determine the level of remating in wild (field-collected) Mediterranean Fruit Fly females from the Greek Island of Chios. We compared the four locus microsatellite genotypes of these females and their offspring. Our data showed 7.1% of wild females remated. Skewed paternity among progeny arrays provided further evidence for double matings. Our lowest estimate of remating was 3.8% and the highest was 21%.
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microsatellite polymorphism in the Mediterranean Fruit Fly ceratitis capitata
Insect Molecular Biology, 2000Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Liangbiao ZhengAbstract:A total of forty-three simple sequence repeats (SSRs) were identified in the Mediterranean Fruit Fly (medFly) Ceratitis capitata . The most common SSR was the dinucleotide (TG) n /(CA) n occurring in thirty of the forty-three microsatellite loci. Polymorphism at ten dinucleotide markers was investigated in 122 flies from six natural populations sampled in the native and colonized areas. A very high level of allelic diversity was detected in the species range. An average of 13.6 alleles was found over all the ten loci indicating the informativeness of SSRs as genetic markers for the medFly. The distribution of microsatellite polymorphism in the species range reflects the medFly colonization history.
C. R. Guglielmino - One of the best experts on this subject based on the ideXlab platform.
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Unfaithful Mediterranean Fruit Fly Ceratitis capitata Females: Impact on the SIT?
Area-Wide Control of Insect Pests, 2020Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, S. Bertin, Francesca Scolari, C. R. Guglielmino, Boaz Yuval, Giuliano Gasperi, Anna R. MalacridaAbstract:An understanding of the levels of remating and paternity skew in the field can be important for polyphagous pest species with a high colonization potential such as the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann). The use of polymorphic simple sequence repeats on flies from two Mediterranean populations in combination with various statistical methods showed not only that Mediterranean Fruit Fly females remate in the wild, but most importantly, that the level of sperm precedence could influence the effect of remating itself since one male, presumably the last, tends to sire most of the progeny. Levels of remating and paternity skew may have important implications for the evolution of the species in terms of maintenance of genetic variability. Moreover, these features of mating behaviour may locally affect the efficiency of the sterile insect technique (SIT), which is a commonly applied control strategy against the Mediterranean Fruit Fly.
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microsatellite analysis reveals remating by wild Mediterranean Fruit Fly females ceratitis capitata
Molecular Ecology, 2002Co-Authors: Mariangela Bonizzoni, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Byron I Katsoyannos, R Marguerie, Tracey ChapmanAbstract:Accurate estimates of remating in wild female insects are required for an understanding of the causes of variation in remating between individuals, populations and species. Such estimates are also of profound importance for major economic Fruit pests such as the Mediterranean Fruit Fly (Ceratitis capitata). A major method for the suppression of this pest is the sterile insect technique (SIT), which relies on matings between mass-reared, sterilized males and wild females. Remating by wild females will thus impact negatively on the success of SIT. We used microsatellite markers to determine the level of remating in wild (field-collected) Mediterranean Fruit Fly females from the Greek Island of Chios. We compared the four locus microsatellite genotypes of these females and their offspring. Our data showed 7.1% of wild females remated. Skewed paternity among progeny arrays provided further evidence for double matings. Our lowest estimate of remating was 3.8% and the highest was 21%.
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microsatellite polymorphism in the Mediterranean Fruit Fly ceratitis capitata
Insect Molecular Biology, 2000Co-Authors: Mariangela Bonizzoni, Ludvik M. Gomulski, C. R. Guglielmino, Giuliano Gasperi, Anna R. Malacrida, Liangbiao ZhengAbstract:A total of forty-three simple sequence repeats (SSRs) were identified in the Mediterranean Fruit Fly (medFly) Ceratitis capitata . The most common SSR was the dinucleotide (TG) n /(CA) n occurring in thirty of the forty-three microsatellite loci. Polymorphism at ten dinucleotide markers was investigated in 122 flies from six natural populations sampled in the native and colonized areas. A very high level of allelic diversity was detected in the species range. An average of 13.6 alleles was found over all the ten loci indicating the informativeness of SSRs as genetic markers for the medFly. The distribution of microsatellite polymorphism in the species range reflects the medFly colonization history.