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Aubrey Bosarge - One of the best experts on this subject based on the ideXlab platform.
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weight loss diet that includes consumption of Medium Chain Triacylglycerol oil leads to a greater rate of weight and fat mass loss than does olive oil
The American Journal of Clinical Nutrition, 2008Co-Authors: Mariepierre Stonge, Aubrey BosargeAbstract:Background Clinical studies have shown that consumption of Medium-Chain Triacylglycerols (MCTs) leads to greater energy expenditure than does consumption of long-Chain Triacylglycerols. Such studies suggest that MCT consumption may be useful for weight management.
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Weight-loss diet that includes consumption of Medium-Chain Triacylglycerol oil leads to a greater rate of weight and fat mass loss than does olive oil
American Journal of Clinical Nutrition, 2008Co-Authors: Marie-pierre St-onge, Aubrey BosargeAbstract:BACKGROUND Clinical studies have shown that consumption of Medium-Chain Triacylglycerols (MCTs) leads to greater energy expenditure than does consumption of long-Chain Triacylglycerols. Such studies suggest that MCT consumption may be useful for weight management. OBJECTIVE We aimed to determine whether consumption of MCT oil improves body weight and fat loss compared with olive oil when consumed as part of a weight-loss program. DESIGN Forty-nine overweight men and women, aged 19-50 y, consumed either 18-24 g/d of MCT oil or olive oil as part of a weight-loss program for 16 wk. Subjects received weekly group weight-loss counseling. Body weight and waist circumference were measured weekly. Adipose tissue distribution was assessed at baseline and at the endpoint by use of dual-energy X-ray absorptiometry and computed tomography. RESULTS Thirty-one subjects completed the study (body mass index: 29.8 +/- 0.4, in kg/m(2)). MCT oil consumption resulted in lower endpoint body weight than did olive oil (-1.67 +/- 0.67 kg, unadjusted P = 0.013). There was a trend toward greater loss of fat mass (P = 0.071) and trunk fat mass (P = 0.10) with MCT consumption than with olive oil. Endpoint trunk fat mass, total fat mass, and intraabdominal adipose tissue were all lower with MCT consumption than with olive oil consumption (all unadjusted P values < 0.05). CONCLUSIONS Consumption of MCT oil as part of a weight-loss plan improves weight loss compared with olive oil and can thus be successfully included in a weight-loss diet. Small changes in the quality of fat intake can therefore be useful to enhance weight loss.
Yvon Carpentier - One of the best experts on this subject based on the ideXlab platform.
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rapid enrichment of cell phospholipids in long Chain polyunsaturated ω 3 fatty acids after a bolus intravenous injection of a Medium Chain Triacylglycerol fish oil emulsion in humans
Journal of Parenteral and Enteral Nutrition, 2012Co-Authors: Yvon Carpentier, Laurence Portois, Willy MalaisseAbstract:The present review aims at highlighting the use of a recently developed Medium-Chain Triacylglycerol:fish oil (MCT:FO) emulsion for the rapid and sustained enrichment of long-Chain polyunsaturated ω-3 fatty acids in cell phospholipids. Preclinical in vitro, in vivo, and ex vivo experiments are briefly considered with emphasis on the changes in the fatty acid pattern of cell phospholipids in several organs, the partial correction of liver steatosis, and the cardiovascular modification of cationic and functional variables observed in ω-3-depleted rats examined 60–120 minutes after a bolus intravenous (IV) injection (1.0 mL) of the MCT:FO emulsion. The clinical findings collected in healthy male volunteers before or after the bolus IV injection (50.0 mL) of either the MCT:FO emulsion or a control Medium-Chain Triacylglycerol:long-Chain Triacylglycerol emulsion are also reviewed, with emphasis on the rapid (within 60 minutes) and sustained (up to 2–3 days) enrichment of platelet and white blood cell phospholi...
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rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel Medium Chain Triacylglycerol fish oil emulsion in humans
The American Journal of Clinical Nutrition, 2010Co-Authors: Yvon Carpentier, Richard J. Deckelbaum, Mirjam Hacquebard, Laurence Portois, Isabelle E Dupont, Willy MalaisseAbstract:Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g Triacylglycerols/100 mL of a mixture of 80% Medium-Chain Triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-Chain Triacylglycerol (MCT:LCT) emulsion containing 20 g Triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean Triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO than for MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr 2010;91:875‐82.
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rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel Medium Chain Triacylglycerol fish oil
2010Co-Authors: Yvon Carpentier, Richard J. Deckelbaum, Mirjam Hacquebard, Laurence Portois, Isabelle E Dupont, Willy MalaisseAbstract:Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g Triacylglycerols/100 mL of a mixture of 80% Medium-Chain Triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-Chain Triacylglycerol (MCT:LCT) emulsion containing 20 g Triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean Triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO compared with MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), was increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr doi: 10.3945/ajcn.2009.27951.
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Inclusion of 10% fish oil in mixed Medium-Chain Triacylglycerol–long-Chain Triacylglycerol emulsions increases plasma Triacylglycerol clearance and induces rapid eicosapentaenoic acid (20:5n−3) incorporation into blood cell phospholipids
The American journal of clinical nutrition, 2008Co-Authors: Christian Simoens, Richard J. Deckelbaum, Jacques J Massaut, Yvon CarpentierAbstract:Background: Lipolysis of a fish oil (FO) emulsion is much slower than that of a soybean [long-Chain Triacylglycerol (LCT)] emulsion; in contrast, emulsions containing Medium-Chain Triacylglycerol (MCT) are efficiently hydrolyzed by lipoprotein lipase. Objectives: We questioned whether incorporating 10% FO in a mixed MCT-LCT emulsion would affect plasma Triacylglycerol clearance and provide efficient delivery of n-3 polyunsaturated fatty acids to cells and tissues. Design: This prospective crossover study was conducted in 8 normolipidemic subjects with the use of the hypertriglyceridemic clamp model and compared plasma Triacylglycerol clearance of a lipid emulsion (5:4:1) made of 50% MCT, 40% LCT, and 10% FO (wt:wt:wt) to a control (5:5) preparation with 50% MCT and 50% LCT. Subjects were daily infused for 5 h, over 4 consecutive days. Fatty acyl pattern was daily measured in plasma phospholipids as well as in leukocyte and platelet phospholipids. Results: Inclusion of 10% FO in mixed emulsion particles enhanced plasma clearance of infused Triacylglycerols (18%; P 2-fold enrichment in leukocytes after 4 infusions. In contrast, the docosahexaenoic acid (C22: 6n-3) concentration remained unchanged in blood cell phospholipids. Conclusions: Infusion of a mixed emulsion with MCTs, soy LCTs, and FO is associated with efficient plasma Triacylglycerol clearance and results in rapid incorporation of C20:5n-3but not C22:6n-3 in leukocyte and platelet phospholipids.
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inclusion of 10 fish oil in mixed Medium Chain Triacylglycerol long Chain Triacylglycerol emulsions increases plasma Triacylglycerol clearance and induces rapid eicosapentaenoic acid 20 5n 3 incorporation into blood cell phospholipids
The American Journal of Clinical Nutrition, 2008Co-Authors: Christian Simoens, Richard J. Deckelbaum, Jacques J Massaut, Yvon CarpentierAbstract:Background: Lipolysis of a fish oil (FO) emulsion is much slower than that of a soybean [long-Chain Triacylglycerol (LCT)] emulsion; in contrast, emulsions containing Medium-Chain Triacylglycerol (MCT) are efficiently hydrolyzed by lipoprotein lipase. Objectives: We questioned whether incorporating 10% FO in a mixed MCT-LCT emulsion would affect plasma Triacylglycerol clearance and provide efficient delivery of n-3 polyunsaturated fatty acids to cells and tissues. Design: This prospective crossover study was conducted in 8 normolipidemic subjects with the use of the hypertriglyceridemic clamp model and compared plasma Triacylglycerol clearance of a lipid emulsion (5:4:1) made of 50% MCT, 40% LCT, and 10% FO (wt:wt:wt) to a control (5:5) preparation with 50% MCT and 50% LCT. Subjects were daily infused for 5 h, over 4 consecutive days. Fatty acyl pattern was daily measured in plasma phospholipids as well as in leukocyte and platelet phospholipids. Results: Inclusion of 10% FO in mixed emulsion particles enhanced plasma clearance of infused Triacylglycerols (18%; P 2-fold enrichment in leukocytes after 4 infusions. In contrast, the docosahexaenoic acid (C22: 6n-3) concentration remained unchanged in blood cell phospholipids. Conclusions: Infusion of a mixed emulsion with MCTs, soy LCTs, and FO is associated with efficient plasma Triacylglycerol clearance and results in rapid incorporation of C20:5n-3but not C22:6n-3 in leukocyte and platelet phospholipids.
Wei-jao Chen - One of the best experts on this subject based on the ideXlab platform.
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effects of a Medium Chain Triacylglycerol long Chain Triacylglycerol fat emulsion containing a reduced ratio of phospholipid to Triacylglycerol in pediatric surgical patients
Nutrition, 2005Co-Authors: Hong-shiee Lai, Wen-hsi Lin, King-jen Chang, Wei-jao ChenAbstract:Abstract Objective Medium-Chain Triacylglycerol (MCT) has been shown to provide better nutritional support than long-Chain Triacylglycerol (LCT). We compared the efficacy of MCT/LCT fat emulsions containing a usual (0.12) or a decreased (0.06) ratio of phospholipid to Triacylglycerol (PL:TG) in pediatric patients under surgical stress. Methods Three patient groups (n = 10 in each) received equivalent amounts of glucose (12 g · kg−1 · d−1) and amino acids (2 g · kg−1 · d−1), but group A received a 10% MCT fat emulsion (PL:TG 0.06), group B received a 20% MCT fat emulsion (PL:TG 0.06), and group C received a 10% MCT/LCT fat emulsion (PL:TG 0.12) in amounts of 1.5 g · kg−1 · d−1 in a randomized study. Total parenteral nutrition was given for 7 d. Blood samples were collected before total parenteral nutrition administration and on days 4 and 7 for determination of various biochemical indexes. Results Serum phospholipid concentrations were significantly higher in group C than in group A or B on days 4 and 7 (P Conclusions In pediatric patients under surgical stress, a total parenteral nutrition regimen containing an MCT/LCT fat emulsion with a decreased PL:TG ratio (0.06) is likely to result in partly better lipid and lipoprotein metabolism than an emulsion containing the usual ratio (0.12).
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Effects of a Medium-Chain Triacylglycerol/long-Chain Triacylglycerol fat emulsion containing a reduced ratio of phospholipid to Triacylglycerol in pediatric surgical patients.
Nutrition, 2005Co-Authors: Hong-shiee Lai, Wen-hsi Lin, King-jen Chang, Wei-jao ChenAbstract:Abstract Objective Medium-Chain Triacylglycerol (MCT) has been shown to provide better nutritional support than long-Chain Triacylglycerol (LCT). We compared the efficacy of MCT/LCT fat emulsions containing a usual (0.12) or a decreased (0.06) ratio of phospholipid to Triacylglycerol (PL:TG) in pediatric patients under surgical stress. Methods Three patient groups (n = 10 in each) received equivalent amounts of glucose (12 g · kg−1 · d−1) and amino acids (2 g · kg−1 · d−1), but group A received a 10% MCT fat emulsion (PL:TG 0.06), group B received a 20% MCT fat emulsion (PL:TG 0.06), and group C received a 10% MCT/LCT fat emulsion (PL:TG 0.12) in amounts of 1.5 g · kg−1 · d−1 in a randomized study. Total parenteral nutrition was given for 7 d. Blood samples were collected before total parenteral nutrition administration and on days 4 and 7 for determination of various biochemical indexes. Results Serum phospholipid concentrations were significantly higher in group C than in group A or B on days 4 and 7 (P Conclusions In pediatric patients under surgical stress, a total parenteral nutrition regimen containing an MCT/LCT fat emulsion with a decreased PL:TG ratio (0.06) is likely to result in partly better lipid and lipoprotein metabolism than an emulsion containing the usual ratio (0.12).
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Effects of a Medium-Chain Triacylglycerol/long-Chain Triacylglycerol fat emulsion containing a reduced ratio of phospholipid to Triacylglycerol in pediatric surgical patients.
Nutrition (Burbank Los Angeles County Calif.), 2005Co-Authors: Hong-shiee Lai, Wen-hsi Lin, King-jen Chang, Wei-jao ChenAbstract:Medium-Chain Triacylglycerol (MCT) has been shown to provide better nutritional support than long-Chain Triacylglycerol (LCT). We compared the efficacy of MCT/LCT fat emulsions containing a usual (0.12) or a decreased (0.06) ratio of phospholipid to Triacylglycerol (PL:TG) in pediatric patients under surgical stress. Three patient groups (n=10 in each) received equivalent amounts of glucose (12 g.kg(-1).d(-1)) and amino acids (2 g.kg(-1).d(-1)), but group A received a 10% MCT fat emulsion (PL:TG 0.06), group B received a 20% MCT fat emulsion (PL:TG 0.06), and group C received a 10% MCT/LCT fat emulsion (PL:TG 0.12) in amounts of 1.5 g.kg(-1).d(-1) in a randomized study. Total parenteral nutrition was given for 7 d. Blood samples were collected before total parenteral nutrition administration and on days 4 and 7 for determination of various biochemical indexes. Serum phospholipid concentrations were significantly higher in group C than in group A or B on days 4 and 7 (P<0.05). Serum Triacylglycerol and cholesterol concentrations and the very-low-density lipoprotein percentage were also significantly higher in group C than in group A or B on days 4 and 7 (P<0.05). The high-density lipoprotein percentage was significantly higher in group B on days 4 and 7 (P<0.05). In pediatric patients under surgical stress, a total parenteral nutrition regimen containing an MCT/LCT fat emulsion with a decreased PL:TG ratio (0.06) is likely to result in partly better lipid and lipoprotein metabolism than an emulsion containing the usual ratio (0.12).
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Applied nutritional investigation Effects of a Medium-Chain Triacylglycerol/long-Chain Triacylglycerol fat emulsion containing a reduced ratio of phospholipid to Triacylglycerol in pediatric surgical patients
2005Co-Authors: Hong-shiee Lai, Wen-hsi Lin, King-jen Chang, Wei-jao ChenAbstract:Objective: Medium-Chain Triacylglycerol (MCT) has been shown to provide better nutritional support than long-Chain Triacylglycerol (LCT). We compared the efficacy of MCT/LCT fat emul- sions containing a usual (0.12) or a decreased (0.06) ratio of phospholipid to Triacylglycerol (PL:TG) in pediatric patients under surgical stress. Methods: Three patient groups (n 10 in each) received equivalent amounts of glucose (12 g·k g 1 ·d 1 ) and amino acids (2 g · kg 1 ·d 1 ), but group A received a 10% MCT fat emulsion (PL:TG 0.06), group B received a 20% MCT fat emulsion (PL:TG 0.06), and group C received a 10% MCT/LCT fat emulsion (PL:TG 0.12) in amounts of 1.5 g · kg 1 ·d 1 in a randomized study. Total parenteral nutrition was given for 7 d. Blood samples were collected before total parenteral nutrition administration and on days 4 and 7 for determination of various biochemical indexes. Results: Serum phospholipid concentrations were significantly higher in group C than in group A or Bo n days 4a nd 7( P 0.05). Serum Triacylglycerol and cholesterol concentrations and the very-low-density lipoprotein percentage were also significantly higher in group C than in group A or Bo n days 4a nd 7( P 0.05). The high-density lipoprotein percentage was significantly higher in group B on days 4 and 7 (P 0.05). Conclusions: In pediatric patients under surgical stress, a total parenteral nutrition regimen containing an MCT/LCT fat emulsion with a decreased PL:TG ratio (0.06) is likely to result in partly better lipid and lipoprotein metabolism than an emulsion containing the usual ratio (0.12). © 2005 Elsevier Inc. All rights reserved.
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applied nutritional investigation effects of a Medium Chain Triacylglycerol long Chain Triacylglycerol fat emulsion containing a reduced ratio of phospholipid to Triacylglycerol in pediatric surgical patients
2005Co-Authors: Hong-shiee Lai, Wen-hsi Lin, King-jen Chang, Wei-jao ChenAbstract:Objective: Medium-Chain Triacylglycerol (MCT) has been shown to provide better nutritional support than long-Chain Triacylglycerol (LCT). We compared the efficacy of MCT/LCT fat emul- sions containing a usual (0.12) or a decreased (0.06) ratio of phospholipid to Triacylglycerol (PL:TG) in pediatric patients under surgical stress. Methods: Three patient groups (n 10 in each) received equivalent amounts of glucose (12 g·k g 1 ·d 1 ) and amino acids (2 g · kg 1 ·d 1 ), but group A received a 10% MCT fat emulsion (PL:TG 0.06), group B received a 20% MCT fat emulsion (PL:TG 0.06), and group C received a 10% MCT/LCT fat emulsion (PL:TG 0.12) in amounts of 1.5 g · kg 1 ·d 1 in a randomized study. Total parenteral nutrition was given for 7 d. Blood samples were collected before total parenteral nutrition administration and on days 4 and 7 for determination of various biochemical indexes. Results: Serum phospholipid concentrations were significantly higher in group C than in group A or Bo n days 4a nd 7( P 0.05). Serum Triacylglycerol and cholesterol concentrations and the very-low-density lipoprotein percentage were also significantly higher in group C than in group A or Bo n days 4a nd 7( P 0.05). The high-density lipoprotein percentage was significantly higher in group B on days 4 and 7 (P 0.05). Conclusions: In pediatric patients under surgical stress, a total parenteral nutrition regimen containing an MCT/LCT fat emulsion with a decreased PL:TG ratio (0.06) is likely to result in partly better lipid and lipoprotein metabolism than an emulsion containing the usual ratio (0.12). © 2005 Elsevier Inc. All rights reserved.
Richard J. Deckelbaum - One of the best experts on this subject based on the ideXlab platform.
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rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel Medium Chain Triacylglycerol fish oil emulsion in humans
The American Journal of Clinical Nutrition, 2010Co-Authors: Yvon Carpentier, Richard J. Deckelbaum, Mirjam Hacquebard, Laurence Portois, Isabelle E Dupont, Willy MalaisseAbstract:Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g Triacylglycerols/100 mL of a mixture of 80% Medium-Chain Triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-Chain Triacylglycerol (MCT:LCT) emulsion containing 20 g Triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean Triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO than for MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr 2010;91:875‐82.
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rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel Medium Chain Triacylglycerol fish oil
2010Co-Authors: Yvon Carpentier, Richard J. Deckelbaum, Mirjam Hacquebard, Laurence Portois, Isabelle E Dupont, Willy MalaisseAbstract:Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g Triacylglycerols/100 mL of a mixture of 80% Medium-Chain Triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-Chain Triacylglycerol (MCT:LCT) emulsion containing 20 g Triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean Triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO compared with MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), was increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr doi: 10.3945/ajcn.2009.27951.
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inclusion of 10 fish oil in mixed Medium Chain Triacylglycerol long Chain Triacylglycerol emulsions increases plasma Triacylglycerol clearance and induces rapid eicosapentaenoic acid 20 5n 3 incorporation into blood cell phospholipids
The American Journal of Clinical Nutrition, 2008Co-Authors: Christian Simoens, Richard J. Deckelbaum, Jacques J Massaut, Yvon CarpentierAbstract:Background: Lipolysis of a fish oil (FO) emulsion is much slower than that of a soybean [long-Chain Triacylglycerol (LCT)] emulsion; in contrast, emulsions containing Medium-Chain Triacylglycerol (MCT) are efficiently hydrolyzed by lipoprotein lipase. Objectives: We questioned whether incorporating 10% FO in a mixed MCT-LCT emulsion would affect plasma Triacylglycerol clearance and provide efficient delivery of n-3 polyunsaturated fatty acids to cells and tissues. Design: This prospective crossover study was conducted in 8 normolipidemic subjects with the use of the hypertriglyceridemic clamp model and compared plasma Triacylglycerol clearance of a lipid emulsion (5:4:1) made of 50% MCT, 40% LCT, and 10% FO (wt:wt:wt) to a control (5:5) preparation with 50% MCT and 50% LCT. Subjects were daily infused for 5 h, over 4 consecutive days. Fatty acyl pattern was daily measured in plasma phospholipids as well as in leukocyte and platelet phospholipids. Results: Inclusion of 10% FO in mixed emulsion particles enhanced plasma clearance of infused Triacylglycerols (18%; P 2-fold enrichment in leukocytes after 4 infusions. In contrast, the docosahexaenoic acid (C22: 6n-3) concentration remained unchanged in blood cell phospholipids. Conclusions: Infusion of a mixed emulsion with MCTs, soy LCTs, and FO is associated with efficient plasma Triacylglycerol clearance and results in rapid incorporation of C20:5n-3but not C22:6n-3 in leukocyte and platelet phospholipids.
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Inclusion of 10% fish oil in mixed Medium-Chain Triacylglycerol–long-Chain Triacylglycerol emulsions increases plasma Triacylglycerol clearance and induces rapid eicosapentaenoic acid (20:5n−3) incorporation into blood cell phospholipids
The American journal of clinical nutrition, 2008Co-Authors: Christian Simoens, Richard J. Deckelbaum, Jacques J Massaut, Yvon CarpentierAbstract:Background: Lipolysis of a fish oil (FO) emulsion is much slower than that of a soybean [long-Chain Triacylglycerol (LCT)] emulsion; in contrast, emulsions containing Medium-Chain Triacylglycerol (MCT) are efficiently hydrolyzed by lipoprotein lipase. Objectives: We questioned whether incorporating 10% FO in a mixed MCT-LCT emulsion would affect plasma Triacylglycerol clearance and provide efficient delivery of n-3 polyunsaturated fatty acids to cells and tissues. Design: This prospective crossover study was conducted in 8 normolipidemic subjects with the use of the hypertriglyceridemic clamp model and compared plasma Triacylglycerol clearance of a lipid emulsion (5:4:1) made of 50% MCT, 40% LCT, and 10% FO (wt:wt:wt) to a control (5:5) preparation with 50% MCT and 50% LCT. Subjects were daily infused for 5 h, over 4 consecutive days. Fatty acyl pattern was daily measured in plasma phospholipids as well as in leukocyte and platelet phospholipids. Results: Inclusion of 10% FO in mixed emulsion particles enhanced plasma clearance of infused Triacylglycerols (18%; P 2-fold enrichment in leukocytes after 4 infusions. In contrast, the docosahexaenoic acid (C22: 6n-3) concentration remained unchanged in blood cell phospholipids. Conclusions: Infusion of a mixed emulsion with MCTs, soy LCTs, and FO is associated with efficient plasma Triacylglycerol clearance and results in rapid incorporation of C20:5n-3but not C22:6n-3 in leukocyte and platelet phospholipids.
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Incorporation of Medium Chain Triacylglycerols into phospholipid bilayers: effect of long Chain Triacylglycerols, cholesterol, and cholesteryl esters.
Journal of Lipid Research, 1996Co-Authors: James A. Hamilton, J.m. Vural, Yvon Carpentier, Richard J. DeckelbaumAbstract:The ability of water-insoluble molecules such as Triacylglycerols to partition from oil phases into phospholipid interfaces may be crucial to their hydrolysis by lipases in the aqueous environment of plasma and cells. This study uses high resolution and magic angle spinning 13C NMR spectros-copy to measure the solubility of the 8-carbon Medium Chain Triacylglycerol, trioctanoin, in the lamellar structure of phospholipids (vesicles and multilayers) in the presence of other neutral lipids that may compete for an interfacial location (long Chain Triacylglycerol, cholesteryl ester, and cholesterol). In the presence of a saturating concentration of triolein (approximately 3 mole%), the solubility of trioctanoin in egg phosphatidylcholine vesicles decreased from 10 mole% to 7 mole%. The presence of a saturating concentration of trioctanoin (approximately 10 mole%) decreased the interfacial solubility of long Chain triolein to approximately 1 mole%. Cholesteryl oleate in phospholipid vesicles slightly diminished the incorporation of trioctanoin into the surface. The presence of cholesterol reduced the interfacial solubility of trioctanoin, but at a high level of cholesterol (30 mole%), trioctanoin had a solubility of 3 mole%. Thus, even in the presence of other competing neutral lipids, Medium Chain Triacylglycerol retains a favorable location and surface concentration for efficient hydrolysis. 13C NMR analysis thus provides an explanation for preferential hydrolysis of Medium, compared to long Chain Triacylglycerol, in a physical blend of Medium and long Chain Triacylglycerol in a single emulsion particle, and in general, a valuable approach to determine substrate availability at phospholipid surfaces.
Willy Malaisse - One of the best experts on this subject based on the ideXlab platform.
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rapid enrichment of cell phospholipids in long Chain polyunsaturated ω 3 fatty acids after a bolus intravenous injection of a Medium Chain Triacylglycerol fish oil emulsion in humans
Journal of Parenteral and Enteral Nutrition, 2012Co-Authors: Yvon Carpentier, Laurence Portois, Willy MalaisseAbstract:The present review aims at highlighting the use of a recently developed Medium-Chain Triacylglycerol:fish oil (MCT:FO) emulsion for the rapid and sustained enrichment of long-Chain polyunsaturated ω-3 fatty acids in cell phospholipids. Preclinical in vitro, in vivo, and ex vivo experiments are briefly considered with emphasis on the changes in the fatty acid pattern of cell phospholipids in several organs, the partial correction of liver steatosis, and the cardiovascular modification of cationic and functional variables observed in ω-3-depleted rats examined 60–120 minutes after a bolus intravenous (IV) injection (1.0 mL) of the MCT:FO emulsion. The clinical findings collected in healthy male volunteers before or after the bolus IV injection (50.0 mL) of either the MCT:FO emulsion or a control Medium-Chain Triacylglycerol:long-Chain Triacylglycerol emulsion are also reviewed, with emphasis on the rapid (within 60 minutes) and sustained (up to 2–3 days) enrichment of platelet and white blood cell phospholi...
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rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel Medium Chain Triacylglycerol fish oil emulsion in humans
The American Journal of Clinical Nutrition, 2010Co-Authors: Yvon Carpentier, Richard J. Deckelbaum, Mirjam Hacquebard, Laurence Portois, Isabelle E Dupont, Willy MalaisseAbstract:Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g Triacylglycerols/100 mL of a mixture of 80% Medium-Chain Triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-Chain Triacylglycerol (MCT:LCT) emulsion containing 20 g Triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean Triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO than for MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr 2010;91:875‐82.
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rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel Medium Chain Triacylglycerol fish oil
2010Co-Authors: Yvon Carpentier, Richard J. Deckelbaum, Mirjam Hacquebard, Laurence Portois, Isabelle E Dupont, Willy MalaisseAbstract:Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g Triacylglycerols/100 mL of a mixture of 80% Medium-Chain Triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-Chain Triacylglycerol (MCT:LCT) emulsion containing 20 g Triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean Triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO compared with MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), was increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr doi: 10.3945/ajcn.2009.27951.