The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform
Subbarao V Madhunapantula - One of the best experts on this subject based on the ideXlab platform.
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comparative assessment of vitamin b12 folic acid and homocysteine levels in relation to p53 expression in Megaloblastic anemia
PLOS ONE, 2016Co-Authors: Manish Kumar Yadav, Nandini M Manoli, Subbarao V MadhunapantulaAbstract:Background Megaloblastic anemia (MBA), also known as macrocytic anemia, is a type of anemia characterized by decreased number of RBCs as well as the presence of unusually large, abnormal and poorly developed erythrocytes (Megaloblasts), which fail to enter blood circulation due to their larger size. Lack of vitamin-B12 (VB12) and / or folate (Vitamin-B9, VB9) with elevated homocysteine is the key factor responsible for Megaloblastic anemia. Prior studies have demonstrated the induction of apoptosis in these abnormal under-developed erythrocytes. However, it is not clear whether this apoptosis induction is due to elevated p53 level or due to any other mechanism. Furthermore, it is also not fully known whether decreased vitamin-B12 and / or folate are responsible for apoptosis induction mediated by p53 in pre-erythroblasts.
Kaoru Tohyama - One of the best experts on this subject based on the ideXlab platform.
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dna ploidy and cell cycle analyses in the bone marrow cells of patients with Megaloblastic anemia using laser scanning cytometry
Cytometry Part B-clinical Cytometry, 2008Co-Authors: Takayuki Tsujioka, Yataro Yoshida, Aki Tochigi, Mitsuyo Kishimoto, Toshinori Kondo, Taizo Tasaka, Hideho Wada, Takashi Sugihara, Kaoru TohyamaAbstract:Background: Megaloblastic anemias are characterized by several hematopoietic cells with dysplastic nuclear morphology. The analyses of DNA ploidy and cell cycle of these cells are important to understand the property of such diseases. Methods: As laser scanning cytometry (LSC) is a useful tool to evaluate the morphology of the cells fixed on the slide glass together with the quantitative analysis of the fluorescence information of each cell by rapid scanning of the specimens, the authors examined the DNA ploidy and cell cycle of six cases with Megaloblastic anemia using LSC. Results: Giant neutrophilic series such as giant metamyelocytes and giant band cells were found to have extraordinarily higher DNA ploidy, while hypersegmented neutrophils represented the normal diploid pattern like normal neutrophils. As to Megaloblasts, cell cycle analysis showed that the proportion of the cells in S phase was increased as compared with the case of normal erythroblasts. Conclusions: The present study clearly demonstrates the abnormal aspects of the hematopoietic cells with Megaloblastic anemia from the viewpoint of the DNA ploidy and cell cycle analyzed by the use of
Manish Kumar Yadav - One of the best experts on this subject based on the ideXlab platform.
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comparative assessment of vitamin b12 folic acid and homocysteine levels in relation to p53 expression in Megaloblastic anemia
PLOS ONE, 2016Co-Authors: Manish Kumar Yadav, Nandini M Manoli, Subbarao V MadhunapantulaAbstract:Background Megaloblastic anemia (MBA), also known as macrocytic anemia, is a type of anemia characterized by decreased number of RBCs as well as the presence of unusually large, abnormal and poorly developed erythrocytes (Megaloblasts), which fail to enter blood circulation due to their larger size. Lack of vitamin-B12 (VB12) and / or folate (Vitamin-B9, VB9) with elevated homocysteine is the key factor responsible for Megaloblastic anemia. Prior studies have demonstrated the induction of apoptosis in these abnormal under-developed erythrocytes. However, it is not clear whether this apoptosis induction is due to elevated p53 level or due to any other mechanism. Furthermore, it is also not fully known whether decreased vitamin-B12 and / or folate are responsible for apoptosis induction mediated by p53 in pre-erythroblasts.
Takayuki Tsujioka - One of the best experts on this subject based on the ideXlab platform.
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dna ploidy and cell cycle analyses in the bone marrow cells of patients with Megaloblastic anemia using laser scanning cytometry
Cytometry Part B-clinical Cytometry, 2008Co-Authors: Takayuki Tsujioka, Yataro Yoshida, Aki Tochigi, Mitsuyo Kishimoto, Toshinori Kondo, Taizo Tasaka, Hideho Wada, Takashi Sugihara, Kaoru TohyamaAbstract:Background: Megaloblastic anemias are characterized by several hematopoietic cells with dysplastic nuclear morphology. The analyses of DNA ploidy and cell cycle of these cells are important to understand the property of such diseases. Methods: As laser scanning cytometry (LSC) is a useful tool to evaluate the morphology of the cells fixed on the slide glass together with the quantitative analysis of the fluorescence information of each cell by rapid scanning of the specimens, the authors examined the DNA ploidy and cell cycle of six cases with Megaloblastic anemia using LSC. Results: Giant neutrophilic series such as giant metamyelocytes and giant band cells were found to have extraordinarily higher DNA ploidy, while hypersegmented neutrophils represented the normal diploid pattern like normal neutrophils. As to Megaloblasts, cell cycle analysis showed that the proportion of the cells in S phase was increased as compared with the case of normal erythroblasts. Conclusions: The present study clearly demonstrates the abnormal aspects of the hematopoietic cells with Megaloblastic anemia from the viewpoint of the DNA ploidy and cell cycle analyzed by the use of
Nandini M Manoli - One of the best experts on this subject based on the ideXlab platform.
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comparative assessment of vitamin b12 folic acid and homocysteine levels in relation to p53 expression in Megaloblastic anemia
PLOS ONE, 2016Co-Authors: Manish Kumar Yadav, Nandini M Manoli, Subbarao V MadhunapantulaAbstract:Background Megaloblastic anemia (MBA), also known as macrocytic anemia, is a type of anemia characterized by decreased number of RBCs as well as the presence of unusually large, abnormal and poorly developed erythrocytes (Megaloblasts), which fail to enter blood circulation due to their larger size. Lack of vitamin-B12 (VB12) and / or folate (Vitamin-B9, VB9) with elevated homocysteine is the key factor responsible for Megaloblastic anemia. Prior studies have demonstrated the induction of apoptosis in these abnormal under-developed erythrocytes. However, it is not clear whether this apoptosis induction is due to elevated p53 level or due to any other mechanism. Furthermore, it is also not fully known whether decreased vitamin-B12 and / or folate are responsible for apoptosis induction mediated by p53 in pre-erythroblasts.