The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Toshiaki Ohshima - One of the best experts on this subject based on the ideXlab platform.
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application of ergothioneine rich extract from an edible mushroom flammulina velutipes for Melanosis prevention in shrimp penaeus monodon and litopenaeus vannamei
Food Research International, 2012Co-Authors: Angel B Encarnacion, Fernand F Fagutao, Orapint Jintasataporn, Wanchai Worawattanamateekul, Ikuo Hirono, Toshiaki OhshimaAbstract:Abstract This report investigated the Melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom ( Flammulina velutipes ) containing 9.13 ± 2.03 mg/mL 2-thiol- l -histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp ( Penaeus monodon ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase ( proPO ) gene in hemocytes. Consequently, the development of Melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu 2+ at the putative binding sites of PPO and proPO , based on copper-chelating activity analyses, thus preventing Melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic Melanosis-inhibiting agents to prevent postmortem Melanosis in shrimp.
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application of ergothioneine rich extract from an edible mushroom flammulina velutipes for Melanosis prevention in shrimp penaeus monodon and litopenaeus vannamei
Food Research International, 2012Co-Authors: Angel B Encarnacion, Fernand F Fagutao, Orapint Jintasataporn, Wanchai Worawattanamateekul, Ikuo Hirono, Toshiaki OhshimaAbstract:Abstract This report investigated the Melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom ( Flammulina velutipes ) containing 9.13 ± 2.03 mg/mL 2-thiol- l -histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp ( Penaeus monodon ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase ( proPO ) gene in hemocytes. Consequently, the development of Melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu 2+ at the putative binding sites of PPO and proPO , based on copper-chelating activity analyses, thus preventing Melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic Melanosis-inhibiting agents to prevent postmortem Melanosis in shrimp.
Frederic Cambazard - One of the best experts on this subject based on the ideXlab platform.
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in vivo confocal microscopic substrate of grey colour in Melanosis
Journal of The European Academy of Dermatology and Venereology, 2015Co-Authors: Elisa Cinotti, Bruno Labeille, Jean-luc Perrot, Frederic Cambazard, C Couzan, C Habougit, E Moscarella, A Kyrgidis, Giuseppe Argenziano, Giovanni PellacaniAbstract:Background Melanosis is the most common cause of mucosal pigmentation and can be clinically difficult to differentiate from early melanoma (MM). Dermoscopy can help in the distinction between Melanosis and MM, but in some instances, melanoses may exhibit overlapping features with MM such as the presence of grey colour. Objective We sought to evaluate whether reflectance confocal microscopy (RCM) can help to better understand the dermoscopic features of melanoses in order to assist clinicians in their diagnosis. Methods All melanoses diagnosed between June 2011 and December 2014 in the Departments of Dermatology of the University of Saint-Etienne (France) and of Modena and Reggio Emilia (Italy), for which dermoscopic and RCM images were available, were included. Twenty-two lesions were biopsied to confirm the clinical diagnosis, whereas the others did not present any change at a follow-up of at least 6 months. The correlation between dermoscopic and RCM features were evaluated by the Spearman's rho correlation coefficient. Results 55 melanoses were studied: 31 of the oral mucosa and 24 of the genital mucosa. 49% (n = 27) of melanoses exhibited a grey colour under dermoscopy. The grey colour correlated with the presence of melanophages under RCM (ρ = 0.424, P = 0.002). Conclusion Our findings highlight that the presence of the grey colour on dermoscopy, considered as an alerting feature, is common in melanoses and it is related to the presence of melanin-laden inflammatory cells in the papillary dermis on RCM. When it is present as a ‘pure’ feature not associated to other colours than brown or to atypical dermoscopical structures, it could be related to the diagnosis of Melanosis.
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Anal Melanosis diagnosed by reflectance confocal microscopy.
Australasian Journal of Dermatology, 2013Co-Authors: Elisa Cinotti, C. Chol, Fabien Forest, Bruno Labeille, Jean-luc Perrot, Frederic CambazardAbstract:: Until now, in vivo reflectance-mode confocal microscopy (IVCM) has been applied only to pigmented lesions of the vulvar and oral mucosa, but not to anal mucosa lesions. We present the first case in which IVCM has been used to diagnose anal Melanosis. Clinical and dermoscopic features were of concern while IVCM found the draped pattern already described for genital Melanosis. IVCM adds information to the clinical and dermatoscopic examination and allows skin biopsies to be avoided. Further studies are needed to define the IVCM features of anal Melanosis and to compare the performance of IVCM with the findings of histological examinations.
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reflectance confocal microscopy for the diagnosis of vulvar melanoma and Melanosis preliminary results
Dermatologic Surgery, 2012Co-Authors: Elisa Cinotti, Bruno Labeille, Jean-luc Perrot, Huges Adegbidi, Frederic CambazardAbstract:Background In the early stages, vulvar melanoma can mimic vulvar Melanosis and therefore the diagnosis is often late and carries a poor prognosis. In vivo reflectance-mode confocal microscopy (RCM) is an emerging technique that allows noninvasive high-resolution imaging of the skin and mucosa, but it has not been employed in the study of genital pigmentation. Objective To analyze the characteristics of vulvar Melanosis and vulvar melanoma using RCM to define the confocal aspects that allow a correct differential diagnosis. Methods and Materials Features of eight melanoses and two melanomas of the vulva were analyzed using RCM. RCM diagnosis was then compared with clinical and histologic diagnosis. Results Two major characteristics are associated with vulvar Melanosis: papillae rimmed by bright monomorphous cells and possible presence of a few dendritic bright cells in the basal layer of the epithelium. Two major features of vulvar melanoma have been identified: atypical cells in the epithelium and loss of normal architecture of chorion papillae. Conclusions Reflectance Confocal Microscopy can play a role in noninvasive differentiation between vulvar melanoma and vulvar Melanosis, but further broader studies are needed to validate our observations.
Angel B Encarnacion - One of the best experts on this subject based on the ideXlab platform.
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application of ergothioneine rich extract from an edible mushroom flammulina velutipes for Melanosis prevention in shrimp penaeus monodon and litopenaeus vannamei
Food Research International, 2012Co-Authors: Angel B Encarnacion, Fernand F Fagutao, Orapint Jintasataporn, Wanchai Worawattanamateekul, Ikuo Hirono, Toshiaki OhshimaAbstract:Abstract This report investigated the Melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom ( Flammulina velutipes ) containing 9.13 ± 2.03 mg/mL 2-thiol- l -histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp ( Penaeus monodon ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase ( proPO ) gene in hemocytes. Consequently, the development of Melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu 2+ at the putative binding sites of PPO and proPO , based on copper-chelating activity analyses, thus preventing Melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic Melanosis-inhibiting agents to prevent postmortem Melanosis in shrimp.
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application of ergothioneine rich extract from an edible mushroom flammulina velutipes for Melanosis prevention in shrimp penaeus monodon and litopenaeus vannamei
Food Research International, 2012Co-Authors: Angel B Encarnacion, Fernand F Fagutao, Orapint Jintasataporn, Wanchai Worawattanamateekul, Ikuo Hirono, Toshiaki OhshimaAbstract:Abstract This report investigated the Melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom ( Flammulina velutipes ) containing 9.13 ± 2.03 mg/mL 2-thiol- l -histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp ( Penaeus monodon ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase ( proPO ) gene in hemocytes. Consequently, the development of Melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu 2+ at the putative binding sites of PPO and proPO , based on copper-chelating activity analyses, thus preventing Melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic Melanosis-inhibiting agents to prevent postmortem Melanosis in shrimp.
Elisa Cinotti - One of the best experts on this subject based on the ideXlab platform.
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in vivo confocal microscopic substrate of grey colour in Melanosis
Journal of The European Academy of Dermatology and Venereology, 2015Co-Authors: Elisa Cinotti, Bruno Labeille, Jean-luc Perrot, Frederic Cambazard, C Couzan, C Habougit, E Moscarella, A Kyrgidis, Giuseppe Argenziano, Giovanni PellacaniAbstract:Background Melanosis is the most common cause of mucosal pigmentation and can be clinically difficult to differentiate from early melanoma (MM). Dermoscopy can help in the distinction between Melanosis and MM, but in some instances, melanoses may exhibit overlapping features with MM such as the presence of grey colour. Objective We sought to evaluate whether reflectance confocal microscopy (RCM) can help to better understand the dermoscopic features of melanoses in order to assist clinicians in their diagnosis. Methods All melanoses diagnosed between June 2011 and December 2014 in the Departments of Dermatology of the University of Saint-Etienne (France) and of Modena and Reggio Emilia (Italy), for which dermoscopic and RCM images were available, were included. Twenty-two lesions were biopsied to confirm the clinical diagnosis, whereas the others did not present any change at a follow-up of at least 6 months. The correlation between dermoscopic and RCM features were evaluated by the Spearman's rho correlation coefficient. Results 55 melanoses were studied: 31 of the oral mucosa and 24 of the genital mucosa. 49% (n = 27) of melanoses exhibited a grey colour under dermoscopy. The grey colour correlated with the presence of melanophages under RCM (ρ = 0.424, P = 0.002). Conclusion Our findings highlight that the presence of the grey colour on dermoscopy, considered as an alerting feature, is common in melanoses and it is related to the presence of melanin-laden inflammatory cells in the papillary dermis on RCM. When it is present as a ‘pure’ feature not associated to other colours than brown or to atypical dermoscopical structures, it could be related to the diagnosis of Melanosis.
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Anal Melanosis diagnosed by reflectance confocal microscopy.
Australasian Journal of Dermatology, 2013Co-Authors: Elisa Cinotti, C. Chol, Fabien Forest, Bruno Labeille, Jean-luc Perrot, Frederic CambazardAbstract:: Until now, in vivo reflectance-mode confocal microscopy (IVCM) has been applied only to pigmented lesions of the vulvar and oral mucosa, but not to anal mucosa lesions. We present the first case in which IVCM has been used to diagnose anal Melanosis. Clinical and dermoscopic features were of concern while IVCM found the draped pattern already described for genital Melanosis. IVCM adds information to the clinical and dermatoscopic examination and allows skin biopsies to be avoided. Further studies are needed to define the IVCM features of anal Melanosis and to compare the performance of IVCM with the findings of histological examinations.
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reflectance confocal microscopy for the diagnosis of vulvar melanoma and Melanosis preliminary results
Dermatologic Surgery, 2012Co-Authors: Elisa Cinotti, Bruno Labeille, Jean-luc Perrot, Huges Adegbidi, Frederic CambazardAbstract:Background In the early stages, vulvar melanoma can mimic vulvar Melanosis and therefore the diagnosis is often late and carries a poor prognosis. In vivo reflectance-mode confocal microscopy (RCM) is an emerging technique that allows noninvasive high-resolution imaging of the skin and mucosa, but it has not been employed in the study of genital pigmentation. Objective To analyze the characteristics of vulvar Melanosis and vulvar melanoma using RCM to define the confocal aspects that allow a correct differential diagnosis. Methods and Materials Features of eight melanoses and two melanomas of the vulva were analyzed using RCM. RCM diagnosis was then compared with clinical and histologic diagnosis. Results Two major characteristics are associated with vulvar Melanosis: papillae rimmed by bright monomorphous cells and possible presence of a few dendritic bright cells in the basal layer of the epithelium. Two major features of vulvar melanoma have been identified: atypical cells in the epithelium and loss of normal architecture of chorion papillae. Conclusions Reflectance Confocal Microscopy can play a role in noninvasive differentiation between vulvar melanoma and vulvar Melanosis, but further broader studies are needed to validate our observations.
Ikuo Hirono - One of the best experts on this subject based on the ideXlab platform.
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application of ergothioneine rich extract from an edible mushroom flammulina velutipes for Melanosis prevention in shrimp penaeus monodon and litopenaeus vannamei
Food Research International, 2012Co-Authors: Angel B Encarnacion, Fernand F Fagutao, Orapint Jintasataporn, Wanchai Worawattanamateekul, Ikuo Hirono, Toshiaki OhshimaAbstract:Abstract This report investigated the Melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom ( Flammulina velutipes ) containing 9.13 ± 2.03 mg/mL 2-thiol- l -histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp ( Penaeus monodon ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase ( proPO ) gene in hemocytes. Consequently, the development of Melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu 2+ at the putative binding sites of PPO and proPO , based on copper-chelating activity analyses, thus preventing Melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic Melanosis-inhibiting agents to prevent postmortem Melanosis in shrimp.
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application of ergothioneine rich extract from an edible mushroom flammulina velutipes for Melanosis prevention in shrimp penaeus monodon and litopenaeus vannamei
Food Research International, 2012Co-Authors: Angel B Encarnacion, Fernand F Fagutao, Orapint Jintasataporn, Wanchai Worawattanamateekul, Ikuo Hirono, Toshiaki OhshimaAbstract:Abstract This report investigated the Melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom ( Flammulina velutipes ) containing 9.13 ± 2.03 mg/mL 2-thiol- l -histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp ( Penaeus monodon ) and Pacific white shrimp ( Litopenaeus vannamei ) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase ( proPO ) gene in hemocytes. Consequently, the development of Melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu 2+ at the putative binding sites of PPO and proPO , based on copper-chelating activity analyses, thus preventing Melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic Melanosis-inhibiting agents to prevent postmortem Melanosis in shrimp.