The Experts below are selected from a list of 210 Experts worldwide ranked by ideXlab platform
H.f. Hildebrand - One of the best experts on this subject based on the ideXlab platform.
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Biological behaviour of an endothelial cell line (HPMEC) on vascular prostheses grafted with hydroxypropylgamma-cyclodextrine (HPγ-CD) and hydroxypropylbeta-cyclodextrine (HPβ-CD)
Journal of Materials Science: Materials in Medicine, 2008Co-Authors: Nicolas Blanchemain, Stéphan Haulon, Michel Traisnel, Michel Morcellet, Bernard Martel, F. Chai, V. Krump-konvalinkova, C. J. Kirkpatrick, H.f. HildebrandAbstract:The cytocompatibility of cyclodextrins (CDs) grafting on vascular polyester (PET) prostheses for further loading with biomolecules was investigated in this study. Viability tests demonstrated no toxicity of HP-CDs and PolyHP-CDs at 4,000 mg/l with survival rates of 80 to 96%. Proliferation tests using the human pulmonary microvascular endothelial cell line (HPMEC-ST1) revealed an excellent biocompatibility for Melinex^® (Film form of PET). For Polythese^® and Polymaille^®, a good proliferation rate was observed at 3 days (60–80%) but decreased at 6 days (56–73%). For all CD-grafted samples, low proliferation rates were observed after 6 days (35–38%). Vitality tests revealed excellent functional capacities of HPMEC cells after 3 and 6 days for all samples. Adhesion kinetics tests showed a similar adhesion of HPMEC cells on control and Melinex^®. A low adhesion was observed on Polythese^® and especially on Polymaille^® compared to control. After CD grafting, the cell adhesion was decreased. The woven or knitted architecture and CD grafting were the most likely causes of this weak adhesion. The adhesion kinetic test was confirmed by SEM observations and immunocytochemistry. The low proliferation of HPMEC on virgin prostheses and especially on grafted prostheses was not due to a cytotoxic effect, but to the physical surface characteristics of the prostheses.
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Vascular prostheses with controlled release of antibiotics Part 2. In vitro biological evaluation of vascular prostheses treated by cyclodextrins.
Biomolecular Engineering, 2006Co-Authors: Nicolas Blanchemain, Stéphan Haulon, Francois Boschin, Michel Traisnel, Michel Morcellet, Bernard Martel, H.f. HildebrandAbstract:Abstract Viability tests by the colony forming method show no toxicity for all CDs (β-CD, γ-CD, HPβ-CD and HPγ-CD) and their associated polymer. A survival rate of 100% is observed for all CDs at high concentration 400 ppm. Proliferation tests revealed a low proliferation of L132 cells on grafted vascular prostheses and untreated prostheses and good proliferation on Melinex® (film form of PET). A proliferation of 17% is observed after 3 days of incubation and decrease at 4% after 6 days on prostheses. Melinex® exhibits a proliferation rate as the controls. Vitality tests confirm proliferation tests and show a good vitality of cells even for low cell amounts. From these experiments it becomes obvious that the decreasing proliferation rate is not a cytotoxic effect but is due to the chemical and/or physical surface characteristics. A similar result is obtained for cell adhesion kinetics between grafted vascular prostheses and control. After 2 h adhesion, a lower adhesion is observed on untreated prostheses. Theses results were confirmed by immunochemistry and morphology tests. This cell adhesion inhibiting effect of the PET prostheses contributes to a better “survival” of vascular prostheses without secondary obstruction or stenosis.
Nicolas Blanchemain - One of the best experts on this subject based on the ideXlab platform.
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Biological behaviour of an endothelial cell line (HPMEC) on vascular prostheses grafted with hydroxypropylgamma-cyclodextrine (HPγ-CD) and hydroxypropylbeta-cyclodextrine (HPβ-CD)
Journal of Materials Science: Materials in Medicine, 2008Co-Authors: Nicolas Blanchemain, Stéphan Haulon, Michel Traisnel, Michel Morcellet, Bernard Martel, F. Chai, V. Krump-konvalinkova, C. J. Kirkpatrick, H.f. HildebrandAbstract:The cytocompatibility of cyclodextrins (CDs) grafting on vascular polyester (PET) prostheses for further loading with biomolecules was investigated in this study. Viability tests demonstrated no toxicity of HP-CDs and PolyHP-CDs at 4,000 mg/l with survival rates of 80 to 96%. Proliferation tests using the human pulmonary microvascular endothelial cell line (HPMEC-ST1) revealed an excellent biocompatibility for Melinex^® (Film form of PET). For Polythese^® and Polymaille^®, a good proliferation rate was observed at 3 days (60–80%) but decreased at 6 days (56–73%). For all CD-grafted samples, low proliferation rates were observed after 6 days (35–38%). Vitality tests revealed excellent functional capacities of HPMEC cells after 3 and 6 days for all samples. Adhesion kinetics tests showed a similar adhesion of HPMEC cells on control and Melinex^®. A low adhesion was observed on Polythese^® and especially on Polymaille^® compared to control. After CD grafting, the cell adhesion was decreased. The woven or knitted architecture and CD grafting were the most likely causes of this weak adhesion. The adhesion kinetic test was confirmed by SEM observations and immunocytochemistry. The low proliferation of HPMEC on virgin prostheses and especially on grafted prostheses was not due to a cytotoxic effect, but to the physical surface characteristics of the prostheses.
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Vascular prostheses with controlled release of antibiotics Part 2. In vitro biological evaluation of vascular prostheses treated by cyclodextrins.
Biomolecular Engineering, 2006Co-Authors: Nicolas Blanchemain, Stéphan Haulon, Francois Boschin, Michel Traisnel, Michel Morcellet, Bernard Martel, H.f. HildebrandAbstract:Abstract Viability tests by the colony forming method show no toxicity for all CDs (β-CD, γ-CD, HPβ-CD and HPγ-CD) and their associated polymer. A survival rate of 100% is observed for all CDs at high concentration 400 ppm. Proliferation tests revealed a low proliferation of L132 cells on grafted vascular prostheses and untreated prostheses and good proliferation on Melinex® (film form of PET). A proliferation of 17% is observed after 3 days of incubation and decrease at 4% after 6 days on prostheses. Melinex® exhibits a proliferation rate as the controls. Vitality tests confirm proliferation tests and show a good vitality of cells even for low cell amounts. From these experiments it becomes obvious that the decreasing proliferation rate is not a cytotoxic effect but is due to the chemical and/or physical surface characteristics. A similar result is obtained for cell adhesion kinetics between grafted vascular prostheses and control. After 2 h adhesion, a lower adhesion is observed on untreated prostheses. Theses results were confirmed by immunochemistry and morphology tests. This cell adhesion inhibiting effect of the PET prostheses contributes to a better “survival” of vascular prostheses without secondary obstruction or stenosis.
Gareth I. Hay - One of the best experts on this subject based on the ideXlab platform.
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Examination of Silver-Graphite Lithographically Printed Resistive Strain Sensors Abstract
2014Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, George Simpson, David J. Harrison, B.j. RamseyAbstract:This paper reports the design and manufacture of three differing types of resistive strain sensitive structures fabricated using the Conductive Lithographic Film (CLF) printing process. The structures, utilising two inks prepared with silver and graphite particulates as the conductive phase, have been analysed to determine electrical and mechanical properties with respect to strain, temperature and humidity when deposited on four alternative substrate materials (GlossArt, PolyArt, Teslin and Melinex)
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Characterization of Lithographically Printed Resistive Strain Gauges
2014Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, George Simpson, David J. Harrison, P.m. HarreyAbstract:Abstract—This paper reports progress in sensor fabrication by the conductive lithographic film (CLF) printing process. Work describing strain-sensitive structures manufactured using a modified printing process and conductive inks is addressed. The performance of a “single-ink ” strain-sensitive structure when printed on six alternative substrates (GlossArt, PolyArt, Teslin, Mylar C, Melinex, and Kapton) is analyzed. Though not intending to compete with conventional gauges in high-tolerance measurement, the structures exhibit properties that indicate suitability for novel applications. Index Terms—Conductive lithographic film (CLF), offset lithography, printed sensors, printed strain gauges. I
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Examination of silver–graphite lithographically printed resistive strain sensors
Sensors and Actuators A-physical, 2007Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, David Harrison, George Simpson, B.j. RamseyAbstract:This paper reports the design and manufacture of three differing types of electrically resistive strain sensitive structures fabricated using the conductive lithographic film (CLF) printing process. The structures, utilising two inks prepared with silver and graphite particulates as the active phase, have been analysed to determine electrical and mechanical properties with respect to strain, temperature and humidity when deposited on four alternative substrate materials (GlossArt, PolyArt, Teslin and Melinex).
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Examination of silver–graphite lithographically printed resistive strain sensors
2007Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, George Simpson, David J. Harrison, B.j. RamseyAbstract:This paper reports the design and manufacture of three differing types of resistive strain sensitive structures fabricated using the Conductive Lithographic Film (CLF) printing process. The structures, utilising two inks prepared with silver and graphite particulates as the conductive phase, have been analysed to determine electrical and mechanical properties with respect to strain, temperature and humidity when deposited on four alternative substrate materials (GlossArt, PolyArt, Teslin and Melinex)
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Characterization of lithographically printed resistive strain gauges
IEEE Sensors Journal, 2005Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, David Harrison, George Simpson, P.m. HarreyAbstract:This paper reports progress in sensor fabrication by the conductive lithographic film (CLF) printing process. Work describing strain-sensitive structures manufactured using a modified printing process and conductive inks is addressed. The performance of a "single-ink" strain-sensitive structure when printed on six alternative substrates (GlossArt, PolyArt, Teslin, Mylar C, Melinex, and Kapton) is analyzed. Though not intending to compete with conventional gauges in high-tolerance measurement, the structures exhibit properties that indicate suitability for novel applications.
P.m. Harrey - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Lithographically Printed Resistive Strain Gauges
2014Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, George Simpson, David J. Harrison, P.m. HarreyAbstract:Abstract—This paper reports progress in sensor fabrication by the conductive lithographic film (CLF) printing process. Work describing strain-sensitive structures manufactured using a modified printing process and conductive inks is addressed. The performance of a “single-ink ” strain-sensitive structure when printed on six alternative substrates (GlossArt, PolyArt, Teslin, Mylar C, Melinex, and Kapton) is analyzed. Though not intending to compete with conventional gauges in high-tolerance measurement, the structures exhibit properties that indicate suitability for novel applications. Index Terms—Conductive lithographic film (CLF), offset lithography, printed sensors, printed strain gauges. I
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Characterization of lithographically printed resistive strain gauges
IEEE Sensors Journal, 2005Co-Authors: Gareth I. Hay, Darren J. Southee, P.s.a. Evans, David Harrison, George Simpson, P.m. HarreyAbstract:This paper reports progress in sensor fabrication by the conductive lithographic film (CLF) printing process. Work describing strain-sensitive structures manufactured using a modified printing process and conductive inks is addressed. The performance of a "single-ink" strain-sensitive structure when printed on six alternative substrates (GlossArt, PolyArt, Teslin, Mylar C, Melinex, and Kapton) is analyzed. Though not intending to compete with conventional gauges in high-tolerance measurement, the structures exhibit properties that indicate suitability for novel applications.
Bernard Martel - One of the best experts on this subject based on the ideXlab platform.
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Biological behaviour of an endothelial cell line (HPMEC) on vascular prostheses grafted with hydroxypropylgamma-cyclodextrine (HPγ-CD) and hydroxypropylbeta-cyclodextrine (HPβ-CD)
Journal of Materials Science: Materials in Medicine, 2008Co-Authors: Nicolas Blanchemain, Stéphan Haulon, Michel Traisnel, Michel Morcellet, Bernard Martel, F. Chai, V. Krump-konvalinkova, C. J. Kirkpatrick, H.f. HildebrandAbstract:The cytocompatibility of cyclodextrins (CDs) grafting on vascular polyester (PET) prostheses for further loading with biomolecules was investigated in this study. Viability tests demonstrated no toxicity of HP-CDs and PolyHP-CDs at 4,000 mg/l with survival rates of 80 to 96%. Proliferation tests using the human pulmonary microvascular endothelial cell line (HPMEC-ST1) revealed an excellent biocompatibility for Melinex^® (Film form of PET). For Polythese^® and Polymaille^®, a good proliferation rate was observed at 3 days (60–80%) but decreased at 6 days (56–73%). For all CD-grafted samples, low proliferation rates were observed after 6 days (35–38%). Vitality tests revealed excellent functional capacities of HPMEC cells after 3 and 6 days for all samples. Adhesion kinetics tests showed a similar adhesion of HPMEC cells on control and Melinex^®. A low adhesion was observed on Polythese^® and especially on Polymaille^® compared to control. After CD grafting, the cell adhesion was decreased. The woven or knitted architecture and CD grafting were the most likely causes of this weak adhesion. The adhesion kinetic test was confirmed by SEM observations and immunocytochemistry. The low proliferation of HPMEC on virgin prostheses and especially on grafted prostheses was not due to a cytotoxic effect, but to the physical surface characteristics of the prostheses.
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Vascular prostheses with controlled release of antibiotics Part 2. In vitro biological evaluation of vascular prostheses treated by cyclodextrins.
Biomolecular Engineering, 2006Co-Authors: Nicolas Blanchemain, Stéphan Haulon, Francois Boschin, Michel Traisnel, Michel Morcellet, Bernard Martel, H.f. HildebrandAbstract:Abstract Viability tests by the colony forming method show no toxicity for all CDs (β-CD, γ-CD, HPβ-CD and HPγ-CD) and their associated polymer. A survival rate of 100% is observed for all CDs at high concentration 400 ppm. Proliferation tests revealed a low proliferation of L132 cells on grafted vascular prostheses and untreated prostheses and good proliferation on Melinex® (film form of PET). A proliferation of 17% is observed after 3 days of incubation and decrease at 4% after 6 days on prostheses. Melinex® exhibits a proliferation rate as the controls. Vitality tests confirm proliferation tests and show a good vitality of cells even for low cell amounts. From these experiments it becomes obvious that the decreasing proliferation rate is not a cytotoxic effect but is due to the chemical and/or physical surface characteristics. A similar result is obtained for cell adhesion kinetics between grafted vascular prostheses and control. After 2 h adhesion, a lower adhesion is observed on untreated prostheses. Theses results were confirmed by immunochemistry and morphology tests. This cell adhesion inhibiting effect of the PET prostheses contributes to a better “survival” of vascular prostheses without secondary obstruction or stenosis.