The Experts below are selected from a list of 46728 Experts worldwide ranked by ideXlab platform
Adam W Perriman - One of the best experts on this subject based on the ideXlab platform.
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artificial cell Membrane binding thrombin constructs drive in situ fibrin hydrogel formation
Nature Communications, 2019Co-Authors: Robert C Deller, Thomas Richardson, Rebecca J Richardson, Laura Bevan, Ioannis Zampetakis, Fabrizio Scarpa, Adam W PerrimanAbstract:Cell Membrane re-engineering is emerging as a powerful tool for the development of next generation cell therapies, as it allows the user to augment therapeutic cells to provide additional functionalities, such as homing, adhesion or hypoxia resistance. To date, however, there are few examples where the plasma Membrane is re-engineered to display active enzymes that promote extracellular matrix protein assembly. Here, we report on a self-contained matrix-forming system where the Membrane of human mesenchymal stem cells is modified to display a novel thrombin construct, giving rise to spontaneous fibrin hydrogel nucleation and growth at near human plasma concentrations of fibrinogen. The cell Membrane Modification process is realised through the synthesis of a Membrane-binding supercationic thrombin-polymer surfactant complex. Significantly, the resulting robust cellular fibrin hydrogel constructs can be differentiated down osteogenic and adipogenic lineages, giving rise to self-supporting monoliths that exhibit Young's moduli that reflect their respective extracellular matrix compositions.
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artificial cell Membrane binding thrombin constructs drive in situ fibrin hydrogel formation
Nature Communications, 2019Co-Authors: Robert C Deller, Thomas Richardson, Rebecca J Richardson, Laura Bevan, Ioannis Zampetakis, Fabrizio Scarpa, Adam W PerrimanAbstract:Cell Membrane re-engineering is emerging as a powerful tool for the development of next generation cell therapies, as it allows the user to augment therapeutic cells to provide additional functionalities, such as homing, adhesion or hypoxia resistance. To date, however, there are few examples where the plasma Membrane is re-engineered to display active enzymes that promote extracellular matrix protein assembly. Here, we report on a self-contained matrix-forming system where the Membrane of human mesenchymal stem cells is modified to display a novel thrombin construct, giving rise to spontaneous fibrin hydrogel nucleation and growth at near human plasma concentrations of fibrinogen. The cell Membrane Modification process is realised through the synthesis of a Membrane-binding supercationic thrombin-polymer surfactant complex. Significantly, the resulting robust cellular fibrin hydrogel constructs can be differentiated down osteogenic and adipogenic lineages, giving rise to self-supporting monoliths that exhibit Young’s moduli that reflect their respective extracellular matrix compositions. The incorporation of cells into tissue engineering scaffolds can be a major challenge. Here, the authors report on anchoring thrombin to cell Membranes for the in situ formation of fibrin scaffolds around the modified cells, demonstrate scaffold formation in vitro and show cell survival in vivo.
Menachem Elimelech - One of the best experts on this subject based on the ideXlab platform.
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thin film composite forward osmosis Membranes functionalized with graphene oxide silver nanocomposites for biofouling control
Journal of Membrane Science, 2017Co-Authors: Andreia Fonseca De Faria, Caihong Liu, Ming Xie, Francois Perreault, Long D Nghiem, Menachem ElimelechAbstract:Innovative approaches to prevent bacterial attachment and biofilm growth on Membranes are critically needed to avoid decreasing Membrane performance due to biofouling. In this study, we propose the fabrication of anti-biofouling thin-film composite Membranes functionalized with graphene oxide–silver nanocomposites. In our Membrane Modification strategy, carboxyl groups on the graphene oxide–silver nanosheets are covalently bonded to carboxyl groups on the surface of thin-film composite Membranes via a crosslinking reaction. Further characterization, such as scanning electron microscopy and Raman spectroscopy, revealed the immobilization of graphene oxide–silver nanocomposites on the Membrane surface. Graphene oxide–silver modified Membranes exhibited an 80% inactivation rate against attached Pseudomonas aeruginosa cells. In addition to a static antimicrobial assay, our study also provided insights on the anti-biofouling property of forward osmosis Membranes during dynamic operation in a cross-flow test cell. Functionalization with graphene oxide–silver nanocomposites resulted in a promising anti-biofouling property without sacrificing the Membrane intrinsic transport properties. Our results demonstrated that the use of graphene oxide–silver nanocomposites is a feasible and attractive approach for the development of anti-biofouling thin-film composite Membranes.
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post fabrication Modification of forward osmosis Membranes with a poly ethylene glycol block copolymer for improved organic fouling resistance
Journal of Membrane Science, 2015Co-Authors: Devin L Shaffer, Humberto Jaramillo, Santiago Romerovargas Castrillon, Xinglin Lu, Menachem ElimelechAbstract:Abstract Facile and effective strategies are needed to modify forward osmosis (FO) Membranes for improved resistance to organic fouling. Fouling resistant FO Membranes will advance the commercial implementation of FO for treating feed waters with high fouling potential, such as wastewater and brines. We report a Membrane Modification technique for post-fabrication grafting of a poly(ethylene glycol) (PEG) block copolymer to the surface of commercial thin-film composite (TFC) FO Membranes via an amide coupling reaction. The PEG concentration for Membrane Modification is optimized based on increased Membrane hydrophilicity and reduced water permeability that result from increasing PEG concentrations during Modification. Modified Membranes exhibit improved resistance to organic fouling compared to unmodified control Membranes when exposed to an aggressive synthetic wastewater mixture. The fouling resistance is achieved despite the non-uniform grafting of PEG, which is attributed to the limited accessibility of carboxylic group binding sites on the Membrane surface. The fouling resistance of Membranes modified using this post-fabrication technique compares favorably to TFC-FO Membranes modified using other procedures. The Modification technique we report in this work has the advantages of being relatively inexpensive, easy to implement, and applicable to commercial Membranes.
Robert C Deller - One of the best experts on this subject based on the ideXlab platform.
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artificial cell Membrane binding thrombin constructs drive in situ fibrin hydrogel formation
Nature Communications, 2019Co-Authors: Robert C Deller, Thomas Richardson, Rebecca J Richardson, Laura Bevan, Ioannis Zampetakis, Fabrizio Scarpa, Adam W PerrimanAbstract:Cell Membrane re-engineering is emerging as a powerful tool for the development of next generation cell therapies, as it allows the user to augment therapeutic cells to provide additional functionalities, such as homing, adhesion or hypoxia resistance. To date, however, there are few examples where the plasma Membrane is re-engineered to display active enzymes that promote extracellular matrix protein assembly. Here, we report on a self-contained matrix-forming system where the Membrane of human mesenchymal stem cells is modified to display a novel thrombin construct, giving rise to spontaneous fibrin hydrogel nucleation and growth at near human plasma concentrations of fibrinogen. The cell Membrane Modification process is realised through the synthesis of a Membrane-binding supercationic thrombin-polymer surfactant complex. Significantly, the resulting robust cellular fibrin hydrogel constructs can be differentiated down osteogenic and adipogenic lineages, giving rise to self-supporting monoliths that exhibit Young's moduli that reflect their respective extracellular matrix compositions.
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artificial cell Membrane binding thrombin constructs drive in situ fibrin hydrogel formation
Nature Communications, 2019Co-Authors: Robert C Deller, Thomas Richardson, Rebecca J Richardson, Laura Bevan, Ioannis Zampetakis, Fabrizio Scarpa, Adam W PerrimanAbstract:Cell Membrane re-engineering is emerging as a powerful tool for the development of next generation cell therapies, as it allows the user to augment therapeutic cells to provide additional functionalities, such as homing, adhesion or hypoxia resistance. To date, however, there are few examples where the plasma Membrane is re-engineered to display active enzymes that promote extracellular matrix protein assembly. Here, we report on a self-contained matrix-forming system where the Membrane of human mesenchymal stem cells is modified to display a novel thrombin construct, giving rise to spontaneous fibrin hydrogel nucleation and growth at near human plasma concentrations of fibrinogen. The cell Membrane Modification process is realised through the synthesis of a Membrane-binding supercationic thrombin-polymer surfactant complex. Significantly, the resulting robust cellular fibrin hydrogel constructs can be differentiated down osteogenic and adipogenic lineages, giving rise to self-supporting monoliths that exhibit Young’s moduli that reflect their respective extracellular matrix compositions. The incorporation of cells into tissue engineering scaffolds can be a major challenge. Here, the authors report on anchoring thrombin to cell Membranes for the in situ formation of fibrin scaffolds around the modified cells, demonstrate scaffold formation in vitro and show cell survival in vivo.
Baoqiang Liao - One of the best experts on this subject based on the ideXlab platform.
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inkjet printing of dopamine followed by uv light irradiation to modify mussel inspired pvdf Membrane for efficient oil water separation
Journal of Membrane Science, 2021Co-Authors: Linhua Rao, Hongjun Lin, Liguo Shen, Jianrong Chen, Baoqiang LiaoAbstract:Abstract Mussel-inspired polydopamine (PDA) Modification of Membrane is an effective alternative for improving permeation flux and anti-fouling performance. Meanwhile, efficient method for oil-water emulsions separation is still highly desired. However, problems, such as time-consuming process and low utilization ratio of expensive raw material, have always been stumbling block to application of this strategy. In this study, inkjet printing of dopamine (DA) followed by UV light irradiation to modify mussel-inspired polyvinylidene fluoride (PVDF) Membrane was proposed. Accordingly, PVDF Membrane was inkjet printed by alternately using DA inks and alkaline tris (hydroxymethyl) aminomethane (Tris) ink. The resultant Membrane was then subjected to photopolymerization under UV irradiation to form a PDA layer. Successful formation of PDA layer on Membrane surface was verified by series of physical and chemical methods. The optimized Membrane (DA80-60/PVDF) exhibited superior oil/water separation performance with 1.5 times permeate flux higher than that of the pristine PVDF Membrane and above 99% oil rejection rate. Meanwhile, the modified Membranes showed satisfactory stability in aqueous solution with wide pH range (pH 2.0–7.0). The novel Membrane Modification method proposed in this study is facile, cost-saving and environment-friendly, serving as a competitive candidate for fabrication of efficient Membranes for oil-water emulsion separation.
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influences of acid base property of Membrane on interfacial interactions related with Membrane fouling in a Membrane bioreactor based on thermodynamic assessment
Bioresource Technology, 2016Co-Authors: Leihong Zhao, Meijia Zhang, Hongjun Lin, Xiaoling Zhou, Baoqiang Liao, Rongwu Mei, Huachang HongAbstract:Abstract Failure of Membrane hydrophobicity in predicting Membrane fouling requires a more reliable indicator. In this study, influences of Membrane acid base (AB) property on interfacial interactions in two different interaction scenarios in a submerged Membrane bioreactor (MBR) were studied according to thermodynamic approaches. It was found that both the polyvinylidene fluoride (PVDF) Membrane and foulant samples in the MBR had relatively high electron donor ( γ − ) component and low electron acceptor ( γ + ) component. For both of interaction scenarios, AB interaction was the major component of the total interaction. The results showed that, the total interaction monotonically decreased with Membrane γ − , while was marginally affected by Membrane γ + , suggesting that γ − could act as a reliable indicator for Membrane fouling prediction. This study suggested that Membrane Modification for fouling mitigation should orient to improving Membrane surface γ − component rather than hydrophilicity.
Yili Lin - One of the best experts on this subject based on the ideXlab platform.
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improving the organic and biological fouling resistance and removal of pharmaceutical and personal care products through nanofiltration by using in situ radical graft polymerization
Science of The Total Environment, 2018Co-Authors: Yili Lin, Chiacheng Tsai, Naiyun ZhengAbstract:In this study, an insitu radical graft polarization technique using monomers of 3-sulfopropyl methacrylate potassium salt (SPM) and 2-hydroxyethyl methacrylate (HEMA) was applied to a commercial nanofiltration Membrane (NF90) to improve its removal of six commonly detected pharmaceutical and personal care products (PPCPs) and mitigate organic and biological fouling by humic acid (HA) and sodium alginate (SA). Compared with the virgin Membrane, the modified NF90 Membrane exhibited considerably improved fouling resistance and an increased reversible fouling percentage, especially for SA+HA composite fouling Moreover, the PPCP removal of the modified NF90 Membrane was higher than that of the virgin Membrane after SA and SA+HA fouling, respectively. Triclosan and carbamazepine, which are poorly rejected, could be effectively removed by modified Membrane after SA or SA+HA fouling. Both monomers modified the Membrane surface by increasing the hydrophilicity and decreasing the contact angle. The degree of grafting was quantified using attenuated total reflection Fourier-transform infrared spectroscopy. The mitigation in the fouling was evident from the low quantity of deposit formed on the modified Membrane, as observed using scanning electron microscopy. A considerable amount of highly hydrophobic triclosan was adsorbed on the SA-fouled virgin Membrane and penetrated through it. By contrast, the adsorption of triclosan was substantially lower in the SPM-modified Membrane. After Membrane Modification, the fouling mechanism changed from solely intermediate blocking to both intermediate blocking and complete blocking after Membrane Modification. Thus, the in situ radical graft polymerization method effectively reduces organic and biological fouling and provides high PPCP removal, which is beneficial for fouling control and produces permeate of satisfactory quality for application in the field of Membrane technology.
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in situ concentration polarization enhanced radical graft polymerization of nf270 for mitigating silica fouling and improving pharmaceutical and personal care product rejection
Journal of Membrane Science, 2018Co-Authors: Yili LinAbstract:Abstract This study aimed to mitigate severe silica fouling and improve the removal efficiency of pharmaceutical and personal care products (PPCPs) through in-situ Modification of a nanofiltration Membrane (NF270) by using the concentration-polymerization-enhanced radical graft polarization method. Two Modification agents were applied with different dosages—3-sulfopropyl methacrylate potassium salt and 2-hydroxyethyl methacrylate. Results show that Membrane Modification can effectively reduce silica fouling compared with virgin NF270, and the flux decline was fully reversible. The permeate flux of modified Membranes increased with increasing monomer concentration due to the increasing degree of grafting and hydrophilicity. The increase slightly compromised the rejection of NaCl, but did not compromise that of PPCP. Instead, PPCP rejection by modified Membranes before silica fouling was significantly higher than that by virgin NF270, especially for neutral carbamazepine. Moreover, Membrane Modification enhanced the rejection of neutral PPCPs after silica fouling by mitigating foulant deposition on the Membrane surface. The fouling mechanism was confirmed to switch from gel layer formation to intermediate blocking or complete blocking. Therefore, the in-situ Modification of NF270 proved to be effective for mitigating silica fouling and improving PPCP rejection.