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Jennifer A Littlechild - One of the best experts on this subject based on the ideXlab platform.

  • Preliminary X-ray analysis of a new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis.
    'International Union of Crystallography (IUCr)', 2015
    Co-Authors: Aa Brindley, Dalby, Andrew R, Mn Isupov, Jennifer A Littlechild
    Abstract:

    Journal ArticleResearch Support, Non-U.S. Gov'tA new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis has been obtained. The crystals exhibit a 'teardrop' morphology and are grown from 2 M ammonium dihydrogen phosphate pH and diffract to beyond 1.7 A resolution. They are in tetragonal space group P4222 with unit-cell dimensions of a = b = 201.9, c = 178.19 A, alpha = beta = gamma = 90 degrees. A 2.3 A resolution native data set has been collected at the Hamburg Synchrotron. A Mercury Derivative data set has also been collected, and the heavy-atom positions have been determined. The self-rotation function and the positions of the heavy atoms are consistent with the molecule being a dodecamer with local 23 symmetry.Biotechnology and Biological Research Council

  • preliminary x ray analysis of a new crystal form of the vanadium dependent bromoperoxidase from corallina officinalis
    Acta Crystallographica Section D-biological Crystallography, 1998
    Co-Authors: Amanda A Brindley, Andrew R Dalby, Michail N Isupov, Jennifer A Littlechild
    Abstract:

    A new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis has been obtained. The crystals exhibit a `teardrop' morphology and are grown from 2 M ammonium dihydrogen phosphate pH 5 and diffract to beyond 1.7 A resolution. They are in tetragonal space group P4222 with unit-cell dimensions of a = b = 201.9, c = 178.19 A, α = β = γ = 90°. A 2.3 A resolution native data set has been collected at the Hamburg Synchrotron. A Mercury Derivative data set has also been collected, and the heavy-atom positions have been determined. The self-rotation function and the positions of the heavy atoms are consistent with the molecule being a dodecamer with local 23 symmetry.

David R Mcmillin - One of the best experts on this subject based on the ideXlab platform.

  • pulsed epr studies of the type 2 copper binding site in the Mercury Derivative of laccase
    Biochemistry, 1992
    Co-Authors: Christopher J Bender, John Mccracken, Jack Peisach, John C Severns, David R Mcmillin
    Abstract:

    The nuclear modulation effect in pulsed EPR spectroscopy was used to study the type 2 copper binding site in the Mercury Derivative of laccase (MDL) in which the type 1 copper is substituted by Hg(II). By comparing the three-pulse electron spin-echo modulations and Fourier transform spectra of MDL and several model compounds, we conclude that the imidazole groups of two histidyl amino acid residues are equatorially coordinated to Cu(II) in the type 2 site. Computer simulations of these data suggest that the remote nonbonding nitrogens of the two imidazoles possess nuclear quadrupole parameters e2qQ = 1.47 MHz and eta = 0.83. A(iso) values of these two nitrogens are not identical, being 1.5 and 2.0 MHz. We have also used samples of the enzyme exchanged with D2O to examine the coordination of the water to the type 2 copper site. The deuterium modulation that is resolved by taking the ratio of the time domain ESEEM data from native and D2O-exchanged enzyme indicates that there is an equatorial water ligand, and further data show that this water is displaced by azide.

Christopher J Bender - One of the best experts on this subject based on the ideXlab platform.

  • pulsed epr studies of the type 2 copper binding site in the Mercury Derivative of laccase
    Biochemistry, 1992
    Co-Authors: Christopher J Bender, John Mccracken, Jack Peisach, John C Severns, David R Mcmillin
    Abstract:

    The nuclear modulation effect in pulsed EPR spectroscopy was used to study the type 2 copper binding site in the Mercury Derivative of laccase (MDL) in which the type 1 copper is substituted by Hg(II). By comparing the three-pulse electron spin-echo modulations and Fourier transform spectra of MDL and several model compounds, we conclude that the imidazole groups of two histidyl amino acid residues are equatorially coordinated to Cu(II) in the type 2 site. Computer simulations of these data suggest that the remote nonbonding nitrogens of the two imidazoles possess nuclear quadrupole parameters e2qQ = 1.47 MHz and eta = 0.83. A(iso) values of these two nitrogens are not identical, being 1.5 and 2.0 MHz. We have also used samples of the enzyme exchanged with D2O to examine the coordination of the water to the type 2 copper site. The deuterium modulation that is resolved by taking the ratio of the time domain ESEEM data from native and D2O-exchanged enzyme indicates that there is an equatorial water ligand, and further data show that this water is displaced by azide.

Amanda A Brindley - One of the best experts on this subject based on the ideXlab platform.

  • preliminary x ray analysis of a new crystal form of the vanadium dependent bromoperoxidase from corallina officinalis
    Acta Crystallographica Section D-biological Crystallography, 1998
    Co-Authors: Amanda A Brindley, Andrew R Dalby, Michail N Isupov, Jennifer A Littlechild
    Abstract:

    A new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis has been obtained. The crystals exhibit a `teardrop' morphology and are grown from 2 M ammonium dihydrogen phosphate pH 5 and diffract to beyond 1.7 A resolution. They are in tetragonal space group P4222 with unit-cell dimensions of a = b = 201.9, c = 178.19 A, α = β = γ = 90°. A 2.3 A resolution native data set has been collected at the Hamburg Synchrotron. A Mercury Derivative data set has also been collected, and the heavy-atom positions have been determined. The self-rotation function and the positions of the heavy atoms are consistent with the molecule being a dodecamer with local 23 symmetry.

John Mccracken - One of the best experts on this subject based on the ideXlab platform.

  • pulsed epr studies of the type 2 copper binding site in the Mercury Derivative of laccase
    Biochemistry, 1992
    Co-Authors: Christopher J Bender, John Mccracken, Jack Peisach, John C Severns, David R Mcmillin
    Abstract:

    The nuclear modulation effect in pulsed EPR spectroscopy was used to study the type 2 copper binding site in the Mercury Derivative of laccase (MDL) in which the type 1 copper is substituted by Hg(II). By comparing the three-pulse electron spin-echo modulations and Fourier transform spectra of MDL and several model compounds, we conclude that the imidazole groups of two histidyl amino acid residues are equatorially coordinated to Cu(II) in the type 2 site. Computer simulations of these data suggest that the remote nonbonding nitrogens of the two imidazoles possess nuclear quadrupole parameters e2qQ = 1.47 MHz and eta = 0.83. A(iso) values of these two nitrogens are not identical, being 1.5 and 2.0 MHz. We have also used samples of the enzyme exchanged with D2O to examine the coordination of the water to the type 2 copper site. The deuterium modulation that is resolved by taking the ratio of the time domain ESEEM data from native and D2O-exchanged enzyme indicates that there is an equatorial water ligand, and further data show that this water is displaced by azide.