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Ruth E Blake - One of the best experts on this subject based on the ideXlab platform.
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Effect of microbial growth rate on temperature and Metabolic Water recorded in 18O/16O ratios of PO4 in DNA
Chemical Geology, 2020Co-Authors: Fei Wang, Hui Li, Chan Yu, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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effect of microbial growth rate on temperature and Metabolic Water recorded in 18o 16o ratios of po4 in dna
Chemical Geology, 2020Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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probing the Metabolic Water contribution to intracellular Water using oxygen isotope ratios of po4
Proceedings of the National Academy of Sciences of the United States of America, 2016Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Knowledge of the relative contributions of different Water sources to intracellular fluids and body Water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body Water composition—including Metabolic Water produced as a byproduct of metabolism—based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, Metabolic Water is not generally considered to be a significant component of intracellular Water, due to the assumed unimpeded diffusion of Water across cell membranes. Here we show that the 18O/16O ratio of PO4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular Water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of Metabolic Water to the intracellular Water of three environmentally diverse strains of bacteria. Our results indicate that ∼30–40% of O in PO4 comprising DNA/biomass in early stationary phase cells is derived from Metabolic Water, which bolsters previous results and also further suggests a constant Metabolic Water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular Water may need to be reconsidered.
Fei Wang - One of the best experts on this subject based on the ideXlab platform.
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Effect of microbial growth rate on temperature and Metabolic Water recorded in 18O/16O ratios of PO4 in DNA
Chemical Geology, 2020Co-Authors: Fei Wang, Hui Li, Chan Yu, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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effect of microbial growth rate on temperature and Metabolic Water recorded in 18o 16o ratios of po4 in dna
Chemical Geology, 2020Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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probing the Metabolic Water contribution to intracellular Water using oxygen isotope ratios of po4
Proceedings of the National Academy of Sciences of the United States of America, 2016Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Knowledge of the relative contributions of different Water sources to intracellular fluids and body Water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body Water composition—including Metabolic Water produced as a byproduct of metabolism—based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, Metabolic Water is not generally considered to be a significant component of intracellular Water, due to the assumed unimpeded diffusion of Water across cell membranes. Here we show that the 18O/16O ratio of PO4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular Water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of Metabolic Water to the intracellular Water of three environmentally diverse strains of bacteria. Our results indicate that ∼30–40% of O in PO4 comprising DNA/biomass in early stationary phase cells is derived from Metabolic Water, which bolsters previous results and also further suggests a constant Metabolic Water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular Water may need to be reconsidered.
Hui Li - One of the best experts on this subject based on the ideXlab platform.
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Effect of microbial growth rate on temperature and Metabolic Water recorded in 18O/16O ratios of PO4 in DNA
Chemical Geology, 2020Co-Authors: Fei Wang, Hui Li, Chan Yu, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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effect of microbial growth rate on temperature and Metabolic Water recorded in 18o 16o ratios of po4 in dna
Chemical Geology, 2020Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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probing the Metabolic Water contribution to intracellular Water using oxygen isotope ratios of po4
Proceedings of the National Academy of Sciences of the United States of America, 2016Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Knowledge of the relative contributions of different Water sources to intracellular fluids and body Water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body Water composition—including Metabolic Water produced as a byproduct of metabolism—based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, Metabolic Water is not generally considered to be a significant component of intracellular Water, due to the assumed unimpeded diffusion of Water across cell membranes. Here we show that the 18O/16O ratio of PO4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular Water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of Metabolic Water to the intracellular Water of three environmentally diverse strains of bacteria. Our results indicate that ∼30–40% of O in PO4 comprising DNA/biomass in early stationary phase cells is derived from Metabolic Water, which bolsters previous results and also further suggests a constant Metabolic Water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular Water may need to be reconsidered.
Sae Jung Chang - One of the best experts on this subject based on the ideXlab platform.
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Effect of microbial growth rate on temperature and Metabolic Water recorded in 18O/16O ratios of PO4 in DNA
Chemical Geology, 2020Co-Authors: Fei Wang, Hui Li, Chan Yu, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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effect of microbial growth rate on temperature and Metabolic Water recorded in 18o 16o ratios of po4 in dna
Chemical Geology, 2020Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
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probing the Metabolic Water contribution to intracellular Water using oxygen isotope ratios of po4
Proceedings of the National Academy of Sciences of the United States of America, 2016Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Knowledge of the relative contributions of different Water sources to intracellular fluids and body Water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body Water composition—including Metabolic Water produced as a byproduct of metabolism—based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, Metabolic Water is not generally considered to be a significant component of intracellular Water, due to the assumed unimpeded diffusion of Water across cell membranes. Here we show that the 18O/16O ratio of PO4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular Water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of Metabolic Water to the intracellular Water of three environmentally diverse strains of bacteria. Our results indicate that ∼30–40% of O in PO4 comprising DNA/biomass in early stationary phase cells is derived from Metabolic Water, which bolsters previous results and also further suggests a constant Metabolic Water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular Water may need to be reconsidered.
Chan Yu - One of the best experts on this subject based on the ideXlab platform.
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Effect of microbial growth rate on temperature and Metabolic Water recorded in 18O/16O ratios of PO4 in DNA
Chemical Geology, 2020Co-Authors: Fei Wang, Hui Li, Chan Yu, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
-
effect of microbial growth rate on temperature and Metabolic Water recorded in 18o 16o ratios of po4 in dna
Chemical Geology, 2020Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Abstract It has been recently demonstrated that both temperature and intracellular/Metabolic Water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular Water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in Water were not significantly different between strains (
-
probing the Metabolic Water contribution to intracellular Water using oxygen isotope ratios of po4
Proceedings of the National Academy of Sciences of the United States of America, 2016Co-Authors: Hui Li, Chan Yu, Fei Wang, Sae Jung Chang, Ruth E BlakeAbstract:Knowledge of the relative contributions of different Water sources to intracellular fluids and body Water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body Water composition—including Metabolic Water produced as a byproduct of metabolism—based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, Metabolic Water is not generally considered to be a significant component of intracellular Water, due to the assumed unimpeded diffusion of Water across cell membranes. Here we show that the 18O/16O ratio of PO4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular Water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of Metabolic Water to the intracellular Water of three environmentally diverse strains of bacteria. Our results indicate that ∼30–40% of O in PO4 comprising DNA/biomass in early stationary phase cells is derived from Metabolic Water, which bolsters previous results and also further suggests a constant Metabolic Water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular Water may need to be reconsidered.