The Experts below are selected from a list of 48 Experts worldwide ranked by ideXlab platform
Ishikawa, Marcia Mayumi - One of the best experts on this subject based on the ideXlab platform.
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Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica
Soc Medicina Veterinaria Estado Rio de Janeiro, 2011Co-Authors: De Padua, Santiago Benites, Ishikawa, Marcia MayumiAbstract:Padua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificacao de basofilos sanguineos em surubim hibrido: contribuicao metodologica. Revista Brasileira de Medicina Veterinaria, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.comDifferent protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for Metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for Metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason
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Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution
Soc Medicina Veterinaria Estado Rio de Janeiro, 2011Co-Authors: De Padua, Santiago Benites [unesp], Ishikawa, Marcia MayumiAbstract:Padua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificacao de basofilos sanguineos em surubim hibrido: contribuicao metodologica. Revista Brasileira de Medicina Veterinaria, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.comDifferent protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for Metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for Metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason
Medini Kanta Pal - One of the best experts on this subject based on the ideXlab platform.
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Spectroscopic probes of the interactions of the dye Stains-all with deoxycholate and cholate
Colloids and Surfaces B: Biointerfaces, 1998Co-Authors: Adrijit Goswami, Medini Kanta PalAbstract:The biological surfactants sodium cholate (NaC) and sodium deoxycholate (NaDC) differ from the normal surfactants such as sodium dodecyl sulphate (SDS) by having hydrophilic -OH groups in their hydrophobic moieties, and they failed to induce sharp blue shifted Metachromasia in the common cationic dyes such as acridine Orange, methylene blue, pinacyanol etc. However, both the cholates induce extremely sharp and stable blue-shifted Metachromasia in the cyanine dye Stains-all (Stal), at concentrate much below the critical micellar concentrations and not disrupted by the excess of surfactants unless at above the respective critical micellar concentrations. Both chiral cholates induce very strong biphasic negative circular dichroism (CD) in Stal. At high surfactant dye both NaDC-Stal and NaC-Stal exhibit a second positive biphasic CD spectrum, indicating the formation of second species of the complexes, not immediately discernible from the respective absorption spectrum. Though the reported structures of micelles and crystals of NaDC are distinctly different from that of NaC, the induced Metachromasia and circular dichroism in Stal by the two surfactants are remarkably similar. It is reasonably thought that Stal cations bound at NaC and NaDC are arranged with systematic twists in one sense, responsible for Metachromasia and dichroism of the dye aggregates, the formation of NaDC-Stal and NaC-Stal are probably followed by some self organization of the complexes formed in the premicellar range of concentrations, excess surfactants added to these complexes form just part of the solvent. Only above cmc the micelles start disruption of the dye aggregates. Our results fit well with the helical model of NaDC and the Small's model of NaDC micellar aggregate with the hydrophobic surfaces oriented inside.
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Circular dichroic and spectrophotometric probes of the competitive binding of crystal violet between DNA and other anionic polymers
Spectrochimica Acta Part A: Molecular Spectroscopy, 1994Co-Authors: Medini Kanta Pal, Jimut Kanti GhoshAbstract:Abstract Of the two synthetic polyanions poly-(styrene sulfonate) (PSS) and poly-(vinyl sulfate) (PVS), the latter induces sharper Metachromasia in the cationic dye crystal violet (CV); DNA, however, fails to induce Metachromasia in the dye. Contrary to speculation, DNA binds CV, though a nonintercalating dye, rather strongly and holds a portion of the dye even in the presence of PSS or PVS, which induces strong Metachromasia. Induced circular dichroism (ICD) in CV by DNA shows that DNA binds CV, and partial reduction of ellipticity of DNA-CV by PVS shows that CV distributes itself between the two polyanions. DNA is shown to be the winner in the competitive binding of CV even with the strong polyanion heparin (Hep) as the competitor. This has been interpreted as being due to the binding of CV to DNA, primarily by ionic attraction stabilized further by nonionic forces like H-bonding in the grooves of DNA. Quantitative estimations of the association constants of DNA-CV and PVS-CV by Scatchard plot come to 0.74 × 10 6 and 1.28 × 10 6 mol −1 , respectively.
Márcia Mayumi Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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METACROMASIA PARA IDENTIFICAÇÃO DE BASÓFILOS SANGUÍNEOS EM SURUBIM HÍBRIDO: CONTRIBUIÇÃO METODOLÓGICA* Metachromasia FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
2011Co-Authors: Santiago Benites De Pádua, Márcia Mayumi IshikawaAbstract:Padua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificacao de basofilos sanguineos em surubim hibrido: contribuicao metodologica. Revista Brasileira de Medicina Veterinaria, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for Metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for Metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.
Kurt Hirschhorn - One of the best experts on this subject based on the ideXlab platform.
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Cellular Metachromasia in Pompe's disease and Pelizaeus‐Merzbacher disease
Clinical Genetics, 2008Co-Authors: Melvin Gertner, Erica Zalay, Kurt HirschhornAbstract:Two additional diseases, Pompe's disease and Pelizaeus-Merzbacher disease, have been shown to demonstrate cellular Metachromasia. In addition, our studies of Metachromasia in fibroblasts from skin biopsies taken from patients with a variety of other disorders are described and our impression of the limited usefulness of Metachromasia is discussed.
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cellular Metachromasia in pompe s disease and pelizaeus merzbacher disease
Clinical Genetics, 2008Co-Authors: Melvin Gertner, Erica Zalay, Kurt HirschhornAbstract:Two additional diseases, Pompe's disease and Pelizaeus-Merzbacher disease, have been shown to demonstrate cellular Metachromasia. In addition, our studies of Metachromasia in fibroblasts from skin biopsies taken from patients with a variety of other disorders are described and our impression of the limited usefulness of Metachromasia is discussed.
Adrijit Goswami - One of the best experts on this subject based on the ideXlab platform.
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Spectroscopic probes of the interactions of the dye Stains-all with deoxycholate and cholate
Colloids and Surfaces B: Biointerfaces, 1998Co-Authors: Adrijit Goswami, Medini Kanta PalAbstract:The biological surfactants sodium cholate (NaC) and sodium deoxycholate (NaDC) differ from the normal surfactants such as sodium dodecyl sulphate (SDS) by having hydrophilic -OH groups in their hydrophobic moieties, and they failed to induce sharp blue shifted Metachromasia in the common cationic dyes such as acridine Orange, methylene blue, pinacyanol etc. However, both the cholates induce extremely sharp and stable blue-shifted Metachromasia in the cyanine dye Stains-all (Stal), at concentrate much below the critical micellar concentrations and not disrupted by the excess of surfactants unless at above the respective critical micellar concentrations. Both chiral cholates induce very strong biphasic negative circular dichroism (CD) in Stal. At high surfactant dye both NaDC-Stal and NaC-Stal exhibit a second positive biphasic CD spectrum, indicating the formation of second species of the complexes, not immediately discernible from the respective absorption spectrum. Though the reported structures of micelles and crystals of NaDC are distinctly different from that of NaC, the induced Metachromasia and circular dichroism in Stal by the two surfactants are remarkably similar. It is reasonably thought that Stal cations bound at NaC and NaDC are arranged with systematic twists in one sense, responsible for Metachromasia and dichroism of the dye aggregates, the formation of NaDC-Stal and NaC-Stal are probably followed by some self organization of the complexes formed in the premicellar range of concentrations, excess surfactants added to these complexes form just part of the solvent. Only above cmc the micelles start disruption of the dye aggregates. Our results fit well with the helical model of NaDC and the Small's model of NaDC micellar aggregate with the hydrophobic surfaces oriented inside.