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Walter J Lukiw - One of the best experts on this subject based on the ideXlab platform.

  • Metal Sulfate induced generation of ros in human brain cells detection using an isomeric mixture of 5 and 6 carboxy 2 7 dichlorofluorescein diacetate carboxy dcfda as a cell permeant tracer
    International Journal of Molecular Sciences, 2012
    Co-Authors: Aileen I Pogue, Maire E Percy, Surjyadipta Bhattacharjee, Yuhai Zhao, Brandon M Jones, Walter J Lukiw
    Abstract:

    Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer’s disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we used mixed isomers of 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (carboxy-DCFDA; C25H14Cl2O9; MW 529.3), a novel fluorescent indicator, to assess ROS generation within human neuronal-glial (HNG) cells in primary co-culture. We introduced pathological stress using the Sulfates of 12 environmentally-, industrially- and agriculturally-relevant divalent and trivalent Metals including Al, Cd, Cu, Fe, Hg, Ga, Mg, Mn, Ni, Pb, Sn and Zn. In this experimental test system, of all the Metal Sulfates analyzed, aluminum Sulfate showed by far the greatest ability to induce intracellular ROS. These studies indicate the utility of using isomeric mixtures of carboxy-H2DCFDA diacetates as novel and highly sensitive, long-lasting, cell-permeant, fluorescein-based tracers for quantifying ROS generation in intact, metabolizing human brain cells, and in analyzing the potential epigenetic contribution of different Metal Sulfates to ROS-generation and ROS-mediated neurological dysfunction.

  • up regulation of nf kb sensitive mirna 125b and mirna 146a in Metal Sulfate stressed human astroglial hag primary cell cultures
    Journal of Inorganic Biochemistry, 2011
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, Surjyadipta Bhattacharjee, James M Hill, T P A Kruck, Yuhai Zhao, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) constitute a unique class of small, non-coding ribonucleic acids (RNAs) that regulate gene expression at the post-transcriptional level. The presence of two inducible miRNAs, miRNA-125b and miRNA-146a, involved in respectively, astroglial cell proliferation and in the innate immune and inflammatory response, is significantly up-regulated in human neurological disorders including Alzheimer's disease (AD). In this study we analyzed abundances miRNA-125b and miRNA-146a in magnesium-, iron-, gallium, and aluminum-Sulfate-stressed human-astroglial (HAG) cells, a structural and immune-responsive brain cell type. The combination of iron- plus aluminum-Sulfate was found to be significantly synergistic in up-regulating reactive oxygen species (ROS) abundance, NF-кB-DNA binding and miRNA-125b and miRNA-146a expression. Treatment of Metal-Sulfate stressed HAG cells with the antioxidant phenyl butyl nitrone (PBN) or the NF-кB inhibitors curcumin, the Metal chelator-anti-oxidant pyrollidine dithiocarbamate (PDTC), or the resveratrol analog CAY10512, abrogated both NF-кB signaling and induction of these miRNAs. Our observations further illustrate the potential of physiologically relevant amounts of aluminum and iron Sulfates to synergistically up-regulate specific miRNAs known to contribute to AD-relevant pathogenetic mechanisms, and suggest that antioxidants or NF-кB inhibitors may be useful to quench Metal-Sulfate triggered genotoxicity.

  • characterization of an nf κb regulated mirna 146a mediated down regulation of complement factor h cfh in Metal Sulfate stressed human brain cells
    Journal of Inorganic Biochemistry, 2009
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, T P A Kruck, Yuhai Zhao, Matthew A Tarr, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) represent a family of small ribonucleic acids (RNAs) that are post-transcriptional regulators of messenger RNA (mRNA) complexity. Brain cells maintain distinct populations of miRNAs that support physiologically normal patterns of expression, however, certain miRNA abundances are significantly altered in neurodegenerative disorders such as Alzheimer's disease (AD). Here we provide evidence in human neural (HN) cells of an aluminum-Sulfate- and reactive oxygen species (ROS)-mediated up-regulation of an NF-kappaB-sensitive miRNA-146a that down-regulates the expression of complement factor H (CFH), an important repressor of inflammation. This NF-kappaB-miRNA-146a-CFH signaling circuit is known to be similarly affected by Abeta42 peptides and in AD brain. These aluminum-Sulfate-inducible events were not observed in parallel experiments using iron-, magnesium-, or zinc-Sulfate-stressed HN cells. An NF-kappaB-containing miRNA-146a-promoter-luciferase reporter construct transfected into HN cells showed significant up-regulation of miRNA-146a after aluminum-Sulfate treatment that corresponded to decreased CFH gene expression. These data suggest that (1) as in AD brain, NF-kappaB-sensitive, miRNA-146a-mediated, modulation of CFH gene expression may contribute to inflammatory responses in aluminum-stressed HN cells, and (2) underscores the potential of nanomolar aluminum to drive genotoxic mechanisms characteristic of neurodegenerative disease processes.

Aileen I Pogue - One of the best experts on this subject based on the ideXlab platform.

  • Metal Sulfate induced generation of ros in human brain cells detection using an isomeric mixture of 5 and 6 carboxy 2 7 dichlorofluorescein diacetate carboxy dcfda as a cell permeant tracer
    International Journal of Molecular Sciences, 2012
    Co-Authors: Aileen I Pogue, Maire E Percy, Surjyadipta Bhattacharjee, Yuhai Zhao, Brandon M Jones, Walter J Lukiw
    Abstract:

    Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer’s disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we used mixed isomers of 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (carboxy-DCFDA; C25H14Cl2O9; MW 529.3), a novel fluorescent indicator, to assess ROS generation within human neuronal-glial (HNG) cells in primary co-culture. We introduced pathological stress using the Sulfates of 12 environmentally-, industrially- and agriculturally-relevant divalent and trivalent Metals including Al, Cd, Cu, Fe, Hg, Ga, Mg, Mn, Ni, Pb, Sn and Zn. In this experimental test system, of all the Metal Sulfates analyzed, aluminum Sulfate showed by far the greatest ability to induce intracellular ROS. These studies indicate the utility of using isomeric mixtures of carboxy-H2DCFDA diacetates as novel and highly sensitive, long-lasting, cell-permeant, fluorescein-based tracers for quantifying ROS generation in intact, metabolizing human brain cells, and in analyzing the potential epigenetic contribution of different Metal Sulfates to ROS-generation and ROS-mediated neurological dysfunction.

  • up regulation of nf kb sensitive mirna 125b and mirna 146a in Metal Sulfate stressed human astroglial hag primary cell cultures
    Journal of Inorganic Biochemistry, 2011
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, Surjyadipta Bhattacharjee, James M Hill, T P A Kruck, Yuhai Zhao, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) constitute a unique class of small, non-coding ribonucleic acids (RNAs) that regulate gene expression at the post-transcriptional level. The presence of two inducible miRNAs, miRNA-125b and miRNA-146a, involved in respectively, astroglial cell proliferation and in the innate immune and inflammatory response, is significantly up-regulated in human neurological disorders including Alzheimer's disease (AD). In this study we analyzed abundances miRNA-125b and miRNA-146a in magnesium-, iron-, gallium, and aluminum-Sulfate-stressed human-astroglial (HAG) cells, a structural and immune-responsive brain cell type. The combination of iron- plus aluminum-Sulfate was found to be significantly synergistic in up-regulating reactive oxygen species (ROS) abundance, NF-кB-DNA binding and miRNA-125b and miRNA-146a expression. Treatment of Metal-Sulfate stressed HAG cells with the antioxidant phenyl butyl nitrone (PBN) or the NF-кB inhibitors curcumin, the Metal chelator-anti-oxidant pyrollidine dithiocarbamate (PDTC), or the resveratrol analog CAY10512, abrogated both NF-кB signaling and induction of these miRNAs. Our observations further illustrate the potential of physiologically relevant amounts of aluminum and iron Sulfates to synergistically up-regulate specific miRNAs known to contribute to AD-relevant pathogenetic mechanisms, and suggest that antioxidants or NF-кB inhibitors may be useful to quench Metal-Sulfate triggered genotoxicity.

  • characterization of an nf κb regulated mirna 146a mediated down regulation of complement factor h cfh in Metal Sulfate stressed human brain cells
    Journal of Inorganic Biochemistry, 2009
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, T P A Kruck, Yuhai Zhao, Matthew A Tarr, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) represent a family of small ribonucleic acids (RNAs) that are post-transcriptional regulators of messenger RNA (mRNA) complexity. Brain cells maintain distinct populations of miRNAs that support physiologically normal patterns of expression, however, certain miRNA abundances are significantly altered in neurodegenerative disorders such as Alzheimer's disease (AD). Here we provide evidence in human neural (HN) cells of an aluminum-Sulfate- and reactive oxygen species (ROS)-mediated up-regulation of an NF-kappaB-sensitive miRNA-146a that down-regulates the expression of complement factor H (CFH), an important repressor of inflammation. This NF-kappaB-miRNA-146a-CFH signaling circuit is known to be similarly affected by Abeta42 peptides and in AD brain. These aluminum-Sulfate-inducible events were not observed in parallel experiments using iron-, magnesium-, or zinc-Sulfate-stressed HN cells. An NF-kappaB-containing miRNA-146a-promoter-luciferase reporter construct transfected into HN cells showed significant up-regulation of miRNA-146a after aluminum-Sulfate treatment that corresponded to decreased CFH gene expression. These data suggest that (1) as in AD brain, NF-kappaB-sensitive, miRNA-146a-mediated, modulation of CFH gene expression may contribute to inflammatory responses in aluminum-stressed HN cells, and (2) underscores the potential of nanomolar aluminum to drive genotoxic mechanisms characteristic of neurodegenerative disease processes.

Maire E Percy - One of the best experts on this subject based on the ideXlab platform.

  • Metal Sulfate induced generation of ros in human brain cells detection using an isomeric mixture of 5 and 6 carboxy 2 7 dichlorofluorescein diacetate carboxy dcfda as a cell permeant tracer
    International Journal of Molecular Sciences, 2012
    Co-Authors: Aileen I Pogue, Maire E Percy, Surjyadipta Bhattacharjee, Yuhai Zhao, Brandon M Jones, Walter J Lukiw
    Abstract:

    Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer’s disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we used mixed isomers of 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (carboxy-DCFDA; C25H14Cl2O9; MW 529.3), a novel fluorescent indicator, to assess ROS generation within human neuronal-glial (HNG) cells in primary co-culture. We introduced pathological stress using the Sulfates of 12 environmentally-, industrially- and agriculturally-relevant divalent and trivalent Metals including Al, Cd, Cu, Fe, Hg, Ga, Mg, Mn, Ni, Pb, Sn and Zn. In this experimental test system, of all the Metal Sulfates analyzed, aluminum Sulfate showed by far the greatest ability to induce intracellular ROS. These studies indicate the utility of using isomeric mixtures of carboxy-H2DCFDA diacetates as novel and highly sensitive, long-lasting, cell-permeant, fluorescein-based tracers for quantifying ROS generation in intact, metabolizing human brain cells, and in analyzing the potential epigenetic contribution of different Metal Sulfates to ROS-generation and ROS-mediated neurological dysfunction.

  • up regulation of nf kb sensitive mirna 125b and mirna 146a in Metal Sulfate stressed human astroglial hag primary cell cultures
    Journal of Inorganic Biochemistry, 2011
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, Surjyadipta Bhattacharjee, James M Hill, T P A Kruck, Yuhai Zhao, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) constitute a unique class of small, non-coding ribonucleic acids (RNAs) that regulate gene expression at the post-transcriptional level. The presence of two inducible miRNAs, miRNA-125b and miRNA-146a, involved in respectively, astroglial cell proliferation and in the innate immune and inflammatory response, is significantly up-regulated in human neurological disorders including Alzheimer's disease (AD). In this study we analyzed abundances miRNA-125b and miRNA-146a in magnesium-, iron-, gallium, and aluminum-Sulfate-stressed human-astroglial (HAG) cells, a structural and immune-responsive brain cell type. The combination of iron- plus aluminum-Sulfate was found to be significantly synergistic in up-regulating reactive oxygen species (ROS) abundance, NF-кB-DNA binding and miRNA-125b and miRNA-146a expression. Treatment of Metal-Sulfate stressed HAG cells with the antioxidant phenyl butyl nitrone (PBN) or the NF-кB inhibitors curcumin, the Metal chelator-anti-oxidant pyrollidine dithiocarbamate (PDTC), or the resveratrol analog CAY10512, abrogated both NF-кB signaling and induction of these miRNAs. Our observations further illustrate the potential of physiologically relevant amounts of aluminum and iron Sulfates to synergistically up-regulate specific miRNAs known to contribute to AD-relevant pathogenetic mechanisms, and suggest that antioxidants or NF-кB inhibitors may be useful to quench Metal-Sulfate triggered genotoxicity.

  • characterization of an nf κb regulated mirna 146a mediated down regulation of complement factor h cfh in Metal Sulfate stressed human brain cells
    Journal of Inorganic Biochemistry, 2009
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, T P A Kruck, Yuhai Zhao, Matthew A Tarr, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) represent a family of small ribonucleic acids (RNAs) that are post-transcriptional regulators of messenger RNA (mRNA) complexity. Brain cells maintain distinct populations of miRNAs that support physiologically normal patterns of expression, however, certain miRNA abundances are significantly altered in neurodegenerative disorders such as Alzheimer's disease (AD). Here we provide evidence in human neural (HN) cells of an aluminum-Sulfate- and reactive oxygen species (ROS)-mediated up-regulation of an NF-kappaB-sensitive miRNA-146a that down-regulates the expression of complement factor H (CFH), an important repressor of inflammation. This NF-kappaB-miRNA-146a-CFH signaling circuit is known to be similarly affected by Abeta42 peptides and in AD brain. These aluminum-Sulfate-inducible events were not observed in parallel experiments using iron-, magnesium-, or zinc-Sulfate-stressed HN cells. An NF-kappaB-containing miRNA-146a-promoter-luciferase reporter construct transfected into HN cells showed significant up-regulation of miRNA-146a after aluminum-Sulfate treatment that corresponded to decreased CFH gene expression. These data suggest that (1) as in AD brain, NF-kappaB-sensitive, miRNA-146a-mediated, modulation of CFH gene expression may contribute to inflammatory responses in aluminum-stressed HN cells, and (2) underscores the potential of nanomolar aluminum to drive genotoxic mechanisms characteristic of neurodegenerative disease processes.

Yuhai Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Metal Sulfate induced generation of ros in human brain cells detection using an isomeric mixture of 5 and 6 carboxy 2 7 dichlorofluorescein diacetate carboxy dcfda as a cell permeant tracer
    International Journal of Molecular Sciences, 2012
    Co-Authors: Aileen I Pogue, Maire E Percy, Surjyadipta Bhattacharjee, Yuhai Zhao, Brandon M Jones, Walter J Lukiw
    Abstract:

    Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer’s disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we used mixed isomers of 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (carboxy-DCFDA; C25H14Cl2O9; MW 529.3), a novel fluorescent indicator, to assess ROS generation within human neuronal-glial (HNG) cells in primary co-culture. We introduced pathological stress using the Sulfates of 12 environmentally-, industrially- and agriculturally-relevant divalent and trivalent Metals including Al, Cd, Cu, Fe, Hg, Ga, Mg, Mn, Ni, Pb, Sn and Zn. In this experimental test system, of all the Metal Sulfates analyzed, aluminum Sulfate showed by far the greatest ability to induce intracellular ROS. These studies indicate the utility of using isomeric mixtures of carboxy-H2DCFDA diacetates as novel and highly sensitive, long-lasting, cell-permeant, fluorescein-based tracers for quantifying ROS generation in intact, metabolizing human brain cells, and in analyzing the potential epigenetic contribution of different Metal Sulfates to ROS-generation and ROS-mediated neurological dysfunction.

  • up regulation of nf kb sensitive mirna 125b and mirna 146a in Metal Sulfate stressed human astroglial hag primary cell cultures
    Journal of Inorganic Biochemistry, 2011
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, Surjyadipta Bhattacharjee, James M Hill, T P A Kruck, Yuhai Zhao, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) constitute a unique class of small, non-coding ribonucleic acids (RNAs) that regulate gene expression at the post-transcriptional level. The presence of two inducible miRNAs, miRNA-125b and miRNA-146a, involved in respectively, astroglial cell proliferation and in the innate immune and inflammatory response, is significantly up-regulated in human neurological disorders including Alzheimer's disease (AD). In this study we analyzed abundances miRNA-125b and miRNA-146a in magnesium-, iron-, gallium, and aluminum-Sulfate-stressed human-astroglial (HAG) cells, a structural and immune-responsive brain cell type. The combination of iron- plus aluminum-Sulfate was found to be significantly synergistic in up-regulating reactive oxygen species (ROS) abundance, NF-кB-DNA binding and miRNA-125b and miRNA-146a expression. Treatment of Metal-Sulfate stressed HAG cells with the antioxidant phenyl butyl nitrone (PBN) or the NF-кB inhibitors curcumin, the Metal chelator-anti-oxidant pyrollidine dithiocarbamate (PDTC), or the resveratrol analog CAY10512, abrogated both NF-кB signaling and induction of these miRNAs. Our observations further illustrate the potential of physiologically relevant amounts of aluminum and iron Sulfates to synergistically up-regulate specific miRNAs known to contribute to AD-relevant pathogenetic mechanisms, and suggest that antioxidants or NF-кB inhibitors may be useful to quench Metal-Sulfate triggered genotoxicity.

  • characterization of an nf κb regulated mirna 146a mediated down regulation of complement factor h cfh in Metal Sulfate stressed human brain cells
    Journal of Inorganic Biochemistry, 2009
    Co-Authors: Aileen I Pogue, Maire E Percy, Jian Guo Cui, T P A Kruck, Yuhai Zhao, Matthew A Tarr, Walter J Lukiw
    Abstract:

    Micro RNAs (miRNAs) represent a family of small ribonucleic acids (RNAs) that are post-transcriptional regulators of messenger RNA (mRNA) complexity. Brain cells maintain distinct populations of miRNAs that support physiologically normal patterns of expression, however, certain miRNA abundances are significantly altered in neurodegenerative disorders such as Alzheimer's disease (AD). Here we provide evidence in human neural (HN) cells of an aluminum-Sulfate- and reactive oxygen species (ROS)-mediated up-regulation of an NF-kappaB-sensitive miRNA-146a that down-regulates the expression of complement factor H (CFH), an important repressor of inflammation. This NF-kappaB-miRNA-146a-CFH signaling circuit is known to be similarly affected by Abeta42 peptides and in AD brain. These aluminum-Sulfate-inducible events were not observed in parallel experiments using iron-, magnesium-, or zinc-Sulfate-stressed HN cells. An NF-kappaB-containing miRNA-146a-promoter-luciferase reporter construct transfected into HN cells showed significant up-regulation of miRNA-146a after aluminum-Sulfate treatment that corresponded to decreased CFH gene expression. These data suggest that (1) as in AD brain, NF-kappaB-sensitive, miRNA-146a-mediated, modulation of CFH gene expression may contribute to inflammatory responses in aluminum-stressed HN cells, and (2) underscores the potential of nanomolar aluminum to drive genotoxic mechanisms characteristic of neurodegenerative disease processes.

Geert Versteeg - One of the best experts on this subject based on the ideXlab platform.

  • the removal of hydrogen sulfide from gas streams using an aqueous Metal Sulfate absorbent part i the absorption of hydrogen sulfide in Metal Sulfate solutions
    Separation and Purification Technology, 2005
    Co-Authors: H Ter Maat, J A Hogendoorn, Geert Versteeg
    Abstract:

    The desulfurization of gas streams using aqueous iron(II)Sulfate (Fe(II)SO4), zinc Sulfate (ZnSO4) and copper Sulfate (CuSO4) solutions as washing liquor is studied theoretically and experimentally. The desulfurization is accomplished by a precipitation reaction that occurs when sulfide ions and Metal ions are brought into contact with each other. A thermodynamic study has been used to determine a theoretical operating window, with respect to the pH of the scrubbing solution, in which the Metal Sulfate solution can react with hydrogen sulfide (H2S), but not with carbon dioxide (CO2) from the gas or hydroxide ions from the scrubbing solution. When the absorption is carried out in this window the proposed process should be capable of removing H2S from the gas stream without uptake of CO2 or the formation of Metal hydroxides. The pH operating window increases in the order of iron, zinc to copper. Experimental verification showed that the proposed process indeed efficiently removes H2S when an aqueous Fe(II)SO4, ZnSO4 or CuSO4 solution is used as absorbent. However, for an efficient desulfurization the lower pH of the experimental pH operating window using the Fe(II)SO4 or ZnSO4 solution was higher than indicated by thermodynamics. The reason for this must probably be attributed to a reduced precipitation rate at decreasing pH. When a CuSO4 solution is used as washing liquor the solution can efficiently remove H2S over the entire pH range studied (as low as pH = 1.4). In this case only the upper pH boundary of the operating window (that indicates the possible formation of copper hydroxide or copper carbonates) seems to be a relevant limit in practice. The laboratory experiments indicate that the absorption of H2S in a CuSO4 solution, at the experimental conditions tested, is a gas phase mass transfer limited process. This allows a high degree of H2S removal in a relatively compact contactor. In addition to the lab scale experiments the potential of the new desulfurization process has also been successfully demonstrated for an industrial biogas using a pilot scale packed bed reactor operated with a fresh and regenerated CuSO4 solution. This study indicates that the precipitation reaction of Metal Sulfates with H2S can be used successfully in a (selective) desulfurization process, and that it can be an attractive alternative to the desulfurization methods currently used.