The Experts below are selected from a list of 323376 Experts worldwide ranked by ideXlab platform
Nicolai Müller - One of the best experts on this subject based on the ideXlab platform.
-
Alternative Pathways of Acetogenic Ethanol and Methanol Degradation in the Thermophilic Anaerobe Thermacetogenium phaeum.
Frontiers in Microbiology, 2019Co-Authors: Anja Keller, Bernhard Schink, Nicolai MüllerAbstract:Growth of the anaerobic thermophile Thermacetogenium phaeum with Methanol, ethanol, ethanolamine, and acetate was investigated in axenic cultures and in syntrophic cultures with Methanothermobacter thermautotrophicus. Microcompartment genes were identified in the T. phaeum genome, and presence of microcompartments was confirmed by transmission electron microscopy and proteome analysis. These genes were expressed only during growth with ethanolamine. Proteome data were compared after growth with all four substrates, and activities of key enzymes of the Wood-Ljungdahl pathway and of enzyme systems leading to production or degradation of acetaldehyde such as alcohol dehydrogenase, aldehyde:ferredoxin oxidoreductase, acetate kinase, and phosphate acetyltransferase were measured in cytoplasmic fractions. Accounting of fermentation stoichiometries and growth yields with all four substrates showed that ethanol and Methanol oxidation follow the same stoichiometries as in Acetobacterium woodii. On the other hand, the pathways of ethanol and Methanol degradations vary between both organisms. Growth yields of T. phaeum were substantially lower than reported for A. woodii. Since T. phaeum has no Rnf complex encoded in its genome, the mechanisms of ATP synthesis have to be different from those of A. woodii. In addition to the central degradation pathways also found in A. woodii, T. phaeum maintains enzyme systems that compensate for the absence of an Rnf-complex but which on the other hand cause a loss of energy. On the basis of our data, pathways of Methanol and ethanol degradation in T. phaeum are discussed.
-
Image_6_Alternative Pathways of Acetogenic Ethanol and Methanol Degradation in the Thermophilic Anaerobe Thermacetogenium phaeum.pdf
2019Co-Authors: Anja Keller, Bernhard Schink, Nicolai MüllerAbstract:Growth of the anaerobic thermophile Thermacetogenium phaeum with Methanol, ethanol, ethanolamine, and acetate was investigated in axenic cultures and in syntrophic cultures with Methanothermobacter thermautotrophicus. Microcompartment genes were identified in the T. phaeum genome, and presence of microcompartments was confirmed by transmission electron microscopy and proteome analysis. These genes were expressed only during growth with ethanolamine. Proteome data were compared after growth with all four substrates, and activities of key enzymes of the Wood–Ljungdahl pathway and of enzyme systems leading to production or degradation of acetaldehyde such as alcohol dehydrogenase, aldehyde:ferredoxin oxidoreductase, acetate kinase, and phosphate acetyltransferase were measured in cytoplasmic fractions. Accounting of fermentation stoichiometries and growth yields with all four substrates showed that ethanol and Methanol oxidation follow the same stoichiometries as in Acetobacterium woodii. On the other hand, the pathways of ethanol and Methanol degradations vary between both organisms. Growth yields of T. phaeum were substantially lower than reported for A. woodii. Since T. phaeum has no Rnf complex encoded in its genome, the mechanisms of ATP synthesis have to be different from those of A. woodii. In addition to the central degradation pathways also found in A. woodii, T. phaeum maintains enzyme systems that compensate for the absence of an Rnf-complex but which on the other hand cause a loss of energy. On the basis of our data, pathways of Methanol and ethanol degradation in T. phaeum are discussed.
Anja Keller - One of the best experts on this subject based on the ideXlab platform.
-
Alternative Pathways of Acetogenic Ethanol and Methanol Degradation in the Thermophilic Anaerobe Thermacetogenium phaeum.
Frontiers in Microbiology, 2019Co-Authors: Anja Keller, Bernhard Schink, Nicolai MüllerAbstract:Growth of the anaerobic thermophile Thermacetogenium phaeum with Methanol, ethanol, ethanolamine, and acetate was investigated in axenic cultures and in syntrophic cultures with Methanothermobacter thermautotrophicus. Microcompartment genes were identified in the T. phaeum genome, and presence of microcompartments was confirmed by transmission electron microscopy and proteome analysis. These genes were expressed only during growth with ethanolamine. Proteome data were compared after growth with all four substrates, and activities of key enzymes of the Wood-Ljungdahl pathway and of enzyme systems leading to production or degradation of acetaldehyde such as alcohol dehydrogenase, aldehyde:ferredoxin oxidoreductase, acetate kinase, and phosphate acetyltransferase were measured in cytoplasmic fractions. Accounting of fermentation stoichiometries and growth yields with all four substrates showed that ethanol and Methanol oxidation follow the same stoichiometries as in Acetobacterium woodii. On the other hand, the pathways of ethanol and Methanol degradations vary between both organisms. Growth yields of T. phaeum were substantially lower than reported for A. woodii. Since T. phaeum has no Rnf complex encoded in its genome, the mechanisms of ATP synthesis have to be different from those of A. woodii. In addition to the central degradation pathways also found in A. woodii, T. phaeum maintains enzyme systems that compensate for the absence of an Rnf-complex but which on the other hand cause a loss of energy. On the basis of our data, pathways of Methanol and ethanol degradation in T. phaeum are discussed.
-
Image_6_Alternative Pathways of Acetogenic Ethanol and Methanol Degradation in the Thermophilic Anaerobe Thermacetogenium phaeum.pdf
2019Co-Authors: Anja Keller, Bernhard Schink, Nicolai MüllerAbstract:Growth of the anaerobic thermophile Thermacetogenium phaeum with Methanol, ethanol, ethanolamine, and acetate was investigated in axenic cultures and in syntrophic cultures with Methanothermobacter thermautotrophicus. Microcompartment genes were identified in the T. phaeum genome, and presence of microcompartments was confirmed by transmission electron microscopy and proteome analysis. These genes were expressed only during growth with ethanolamine. Proteome data were compared after growth with all four substrates, and activities of key enzymes of the Wood–Ljungdahl pathway and of enzyme systems leading to production or degradation of acetaldehyde such as alcohol dehydrogenase, aldehyde:ferredoxin oxidoreductase, acetate kinase, and phosphate acetyltransferase were measured in cytoplasmic fractions. Accounting of fermentation stoichiometries and growth yields with all four substrates showed that ethanol and Methanol oxidation follow the same stoichiometries as in Acetobacterium woodii. On the other hand, the pathways of ethanol and Methanol degradations vary between both organisms. Growth yields of T. phaeum were substantially lower than reported for A. woodii. Since T. phaeum has no Rnf complex encoded in its genome, the mechanisms of ATP synthesis have to be different from those of A. woodii. In addition to the central degradation pathways also found in A. woodii, T. phaeum maintains enzyme systems that compensate for the absence of an Rnf-complex but which on the other hand cause a loss of energy. On the basis of our data, pathways of Methanol and ethanol degradation in T. phaeum are discussed.
R Hidalgoalvarez - One of the best experts on this subject based on the ideXlab platform.
-
streaming current permeability and microelectrophoresis of polystyrene latices in Methanol ethanol mixtures
Journal of Colloid and Interface Science, 2004Co-Authors: O Elgholabzouri, M A Cabrerizovilchez, R HidalgoalvarezAbstract:Abstract The influence of the solvent (Methanol–ethanol mixtures) on the electrokinetic behavior of polystyrene latices with sulfate groups was studied (Methanol content was increased by 0.2 at a constant KBr concentration of 1 mM). Viscosity, density, and dielectric constant ( η , ρ , and e ) were determined at experimental conditions. Two latices (with different surface charge densities and sizes) were used. Electrophoresis measurements were used for dilute dispersions. Streaming current and hydrodynamic permeability were measured for porous plugs. Linear trends in the electrokinetic measurements were observed in the whole molar fraction range. The experimental data obtained from different techniques allow determining the zeta potential according to a well-established classical relationship. The results obtained were analyzed on the basis of the solvent mixture properties and the electrical interface behavior. In addition, permeability data provided valuable information to interpret effects at the solid–liquid interface of the porous plug.
-
streaming current permeability and microelectrophoresis of polystyrene latices in Methanol ethanol mixtures
Journal of Colloid and Interface Science, 2004Co-Authors: O Elgholabzouri, M A Cabrerizovilchez, R HidalgoalvarezAbstract:The influence of the solvent (Methanol-ethanol mixtures) on the electrokinetic behavior of polystyrene latices with sulfate groups was studied (Methanol content was increased by 0.2 at a constant KBr concentration of 1 mM). Viscosity, density, and dielectric constant (eta, rho, and epsilon) were determined at experimental conditions. Two latices (with different surface charge densities and sizes) were used. Electrophoresis measurements were used for dilute dispersions. Streaming current and hydrodynamic permeability were measured for porous plugs. Linear trends in the electrokinetic measurements were observed in the whole molar fraction range. The experimental data obtained from different techniques allow determining the zeta potential according to a well-established classical relationship. The results obtained were analyzed on the basis of the solvent mixture properties and the electrical interface behavior. In addition, permeability data provided valuable information to interpret effects at the solid-liquid interface of the porous plug.
Ana Soto - One of the best experts on this subject based on the ideXlab platform.
-
isobaric vapor liquid equilibria of Methanol hexyl acetate and ethanol hexyl acetate
Journal of Chemical & Engineering Data, 1995Co-Authors: Alberto Arce, Antonio Blanco, Jose Martinezageitos, Ana SotoAbstract:Vapor-liquid equilibria of Methanol + hexyl acetate and ethanol + hexyl acetate mixtures were determined at 101.32 kPa. The data were checked for thermodynamic consistency using Fredenslund's test, correlated using Wilson, NRTL, and UNIQUAC equations for the liquid phase activity coefficients, and compared with the predictions of the group contribution methods UNIFAC and ASOG.
László Pusztai - One of the best experts on this subject based on the ideXlab platform.
-
Hydrogen Bonding and Molecular Aggregates in Liquid Methanol, Ethanol, and 1-Propanol
The journal of physical chemistry. B, 2011Co-Authors: Aleksander Vrhovšek, Orsolya Gereben, Andrej Jamnik, László PusztaiAbstract:We present a detailed and comprehensive structural study of molecular models of liquid Methanol, ethanol, and 1-propanol that originate from a series of reverse Monte Carlo (RMC), molecular dynamic...
