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Teresa Gardecerdan - One of the best experts on this subject based on the ideXlab platform.

  • improvement of grape and wine phenolic content by foliar application to grapevine of three different elicitors Methyl Jasmonate chitosan and yeast extract
    Food Chemistry, 2016
    Co-Authors: Javier Portu, Pilar Santamaría, Rosa Lopez, Elisa Baroja, Teresa Gardecerdan
    Abstract:

    Phenolic compounds play a key role in grape and wine organoleptic properties, being therefore a key parameter in wine quality. Elicitor application constitutes an interesting field of research since it is indirectly involved in the accumulation of phenolic compounds. The aim of this study was to compare the effect of the application of three different elicitors on both grape and wine phenolic content. Methyl Jasmonate, chitosan, and a commercial yeast extract were applied to the canopy at veraison and one week later. Results showed that foliar treatments carried out with Methyl Jasmonate and yeast extract achieved the best results, increasing grape and wine anthocyanin content when compared to the control. Moreover, the application of the yeast elicitor also enhanced grape stilbene content. In contrast, the chitosan treatment did not have a substantial impact on the phenolic compounds. The results of this study indicate that Methyl Jasmonate and yeast extract applications could be a simple practice to increase grape and wine phenolic content.

  • Methyl Jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Javier Portu, Pilar Santamaría, Isabel Lopezalfaro, Rosa Lopez, Teresa Gardecerdan
    Abstract:

    The importance of phenolic compounds for grape and wine quality has drawn attention to studying different practices with the aim of increasing their content. Cluster application of elicitors is a viticultural practice that has shown promising results in recent years. However, cluster application requires a previous defoliation, which is time-consuming and expensive. In the present study, Methyl Jasmonate was foliar applied to Tempranillo grapevines in order to study its effect on grape and wine phenolic composition. Methyl Jasmonate foliar application increased anthocyanin and stilbene content in both grape and wine, besides enhancing wine flavonol content. This treatment induced the synthesis of 3-O-glucosides of petunidin and peonidin and trans-p-coumaroyl derivatives of cyanidin and peonidin. For stilbenes, trans-piceid content was considerably increased in both grape and wine. The results obtained suggest that Methyl Jasmonate foliar application could be a simple and accessible practice to enhance gra...

Clarence A. Ryan - One of the best experts on this subject based on the ideXlab platform.

  • wound inducible proteinase inhibitors in pepper differential regulation upon wounding systemin and Methyl Jasmonate
    Plant Physiology, 2001
    Co-Authors: Daniel S Moura, Clarence A. Ryan
    Abstract:

    Seven small (approximately 6,000 D) wound-inducible proteinase inhibitor proteins were isolated from leaves of pepper ( Capsicum annuum ) plants that are members of the potato inhibitor II family. N-terminal sequences obtained indicated that the pepper leaf proteinase inhibitors (PLPIs) exhibit homology to two GenBank accessions that code for preproteins containing three isoinhibitors domains each that, when post-translationally processed, can account for the mixture of isoinhibitors that are reported herein from pepper leaves. A constitutive level of PLPI proteins was found in pepper leaves, and these levels increased up to 2.6-fold upon wounding of the lower leaves. Exposing intact plants to Methyl Jasmonate vapors induced the accumulation of PLPIs. Supplying excised young pepper plants with water through the cut stems induced PLPI proteins to levels higher than those found in intact plants, but with high variability. Supplying the excised plants with systemin did not result in an increase of PLPI levels that were statistically higher than levels found in excised plants. Gel-blot analyses of PLPI induction revealed the presence of two mRNA bands, having slightly different mobilities in agarose gels. Only the low M r mRNA is present in untreated control plants, and it appears to be responsible for the constitutive levels of PLPI found in leaves. Both mRNA species are wound- and Methyl Jasmonate-inducible. Only the low- M r species is weakly induced by systemin, indicating a differential expression of the two PLPI species.

  • a survey of wound and Methyl Jasmonate induced leaf polyphenol oxidase in crop plants
    Phytochemistry, 1998
    Co-Authors: Peter C Constabel, Clarence A. Ryan
    Abstract:

    Polyphenol oxidases (PPOs) are widespread enzymes which oxidize plant phenolic compounds. In tomato leaves, PPO is systemically wound-induced, regulated by the tomato wound signal systemin via the octadecanoid wound-signalling pathway, and appears to function as an anti-nutritive defense against folivore insect pests. In order to determine if PPO could be important for the induced defense of other crop plants, plants from five plant families were surveyed for constitutive, wound-induced, and Methyl Jasmonate-induced PPO activity. In addition to tomato, PPO was most strongly induced by wounding or Methyl Jasmonate in tobacco and hybrid poplar. In these plants the response was also shown to be systemically induced by wounding. Two species, willow and soybean, had very high constitutive PPO activities yet showed no significant PPO induction. Most of the species tested had low constitutive levels of PPO activity, and low or no induction by wounding or Methyl Jasmonate. Although PPO is widely distributed, only in some species does it appear to be a component of the inducible anti-herbivore defense.

  • Regulation of Expression of Proteinase Inhibitor Genes by Methyl Jasmonate and Jasmonic Acid
    Plant physiology, 1992
    Co-Authors: Edward E. Farmer, Russell R. Johnson, Clarence A. Ryan
    Abstract:

    Gel electrophoretic analysis of the proteinase inhibitor proteins induced in tomato leaves by airborne Methyl Jasmonate (EE Farmer, CA Ryan [1990] Proc Natl Acad Sci USA 87: 7713-7716) revealed the new appearance of inhibitors I and II and two other, higher molecular mass proteins (63.5 and 87 kilodaltons). Northern analysis of Methyl Jasmonate-induced inhibitors I and II mRNAs in tomato (Lycopersicon esculentum) leaves, and of alfalfa trypsin inhibitor (a Bowman-Birk family inhibitor) mRNA in alfalfa (Medicago sativa) leaves, indicated that nascent inhibitor mRNAs were regulated in a manner similar to wounding, that is, at the transcriptional level. In tobacco (Nicotiana tabacum), transformed with a fused gene composed of the 5' and 3' regions of a wound-inducible potato inhibitor II and a chloramphenicol acetyl transferase (CAT) gene coding region, CAT activity was induced in leaves by Methyl Jasmonate, consistent with a transcriptional regulation of the inhibitor II gene. In tomato leaves, inhibitor I and II mRNAs and proteins accumulated in leaves distal to those exposed to Methyl Jasmonate or jasmonic acid to similar levels as in exposed leaves. We suggest that in response to wound signals generated by insect or pathogen attacks, linolenic acid is released into the cytoplasm from plant cell membrane lipids and is rapidly converted in cells to jasmonic acid (or perhaps a closely related derivative such as Methyl Jasmonate), which serves as a signal to regulate the expression of proteinase inhibitor genes.

Javier Portu - One of the best experts on this subject based on the ideXlab platform.

  • improvement of grape and wine phenolic content by foliar application to grapevine of three different elicitors Methyl Jasmonate chitosan and yeast extract
    Food Chemistry, 2016
    Co-Authors: Javier Portu, Pilar Santamaría, Rosa Lopez, Elisa Baroja, Teresa Gardecerdan
    Abstract:

    Phenolic compounds play a key role in grape and wine organoleptic properties, being therefore a key parameter in wine quality. Elicitor application constitutes an interesting field of research since it is indirectly involved in the accumulation of phenolic compounds. The aim of this study was to compare the effect of the application of three different elicitors on both grape and wine phenolic content. Methyl Jasmonate, chitosan, and a commercial yeast extract were applied to the canopy at veraison and one week later. Results showed that foliar treatments carried out with Methyl Jasmonate and yeast extract achieved the best results, increasing grape and wine anthocyanin content when compared to the control. Moreover, the application of the yeast elicitor also enhanced grape stilbene content. In contrast, the chitosan treatment did not have a substantial impact on the phenolic compounds. The results of this study indicate that Methyl Jasmonate and yeast extract applications could be a simple practice to increase grape and wine phenolic content.

  • Methyl Jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Javier Portu, Pilar Santamaría, Isabel Lopezalfaro, Rosa Lopez, Teresa Gardecerdan
    Abstract:

    The importance of phenolic compounds for grape and wine quality has drawn attention to studying different practices with the aim of increasing their content. Cluster application of elicitors is a viticultural practice that has shown promising results in recent years. However, cluster application requires a previous defoliation, which is time-consuming and expensive. In the present study, Methyl Jasmonate was foliar applied to Tempranillo grapevines in order to study its effect on grape and wine phenolic composition. Methyl Jasmonate foliar application increased anthocyanin and stilbene content in both grape and wine, besides enhancing wine flavonol content. This treatment induced the synthesis of 3-O-glucosides of petunidin and peonidin and trans-p-coumaroyl derivatives of cyanidin and peonidin. For stilbenes, trans-piceid content was considerably increased in both grape and wine. The results obtained suggest that Methyl Jasmonate foliar application could be a simple and accessible practice to enhance gra...

Rosa Lopez - One of the best experts on this subject based on the ideXlab platform.

  • improvement of grape and wine phenolic content by foliar application to grapevine of three different elicitors Methyl Jasmonate chitosan and yeast extract
    Food Chemistry, 2016
    Co-Authors: Javier Portu, Pilar Santamaría, Rosa Lopez, Elisa Baroja, Teresa Gardecerdan
    Abstract:

    Phenolic compounds play a key role in grape and wine organoleptic properties, being therefore a key parameter in wine quality. Elicitor application constitutes an interesting field of research since it is indirectly involved in the accumulation of phenolic compounds. The aim of this study was to compare the effect of the application of three different elicitors on both grape and wine phenolic content. Methyl Jasmonate, chitosan, and a commercial yeast extract were applied to the canopy at veraison and one week later. Results showed that foliar treatments carried out with Methyl Jasmonate and yeast extract achieved the best results, increasing grape and wine anthocyanin content when compared to the control. Moreover, the application of the yeast elicitor also enhanced grape stilbene content. In contrast, the chitosan treatment did not have a substantial impact on the phenolic compounds. The results of this study indicate that Methyl Jasmonate and yeast extract applications could be a simple practice to increase grape and wine phenolic content.

  • Methyl Jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Javier Portu, Pilar Santamaría, Isabel Lopezalfaro, Rosa Lopez, Teresa Gardecerdan
    Abstract:

    The importance of phenolic compounds for grape and wine quality has drawn attention to studying different practices with the aim of increasing their content. Cluster application of elicitors is a viticultural practice that has shown promising results in recent years. However, cluster application requires a previous defoliation, which is time-consuming and expensive. In the present study, Methyl Jasmonate was foliar applied to Tempranillo grapevines in order to study its effect on grape and wine phenolic composition. Methyl Jasmonate foliar application increased anthocyanin and stilbene content in both grape and wine, besides enhancing wine flavonol content. This treatment induced the synthesis of 3-O-glucosides of petunidin and peonidin and trans-p-coumaroyl derivatives of cyanidin and peonidin. For stilbenes, trans-piceid content was considerably increased in both grape and wine. The results obtained suggest that Methyl Jasmonate foliar application could be a simple and accessible practice to enhance gra...

Pilar Santamaría - One of the best experts on this subject based on the ideXlab platform.

  • improvement of grape and wine phenolic content by foliar application to grapevine of three different elicitors Methyl Jasmonate chitosan and yeast extract
    Food Chemistry, 2016
    Co-Authors: Javier Portu, Pilar Santamaría, Rosa Lopez, Elisa Baroja, Teresa Gardecerdan
    Abstract:

    Phenolic compounds play a key role in grape and wine organoleptic properties, being therefore a key parameter in wine quality. Elicitor application constitutes an interesting field of research since it is indirectly involved in the accumulation of phenolic compounds. The aim of this study was to compare the effect of the application of three different elicitors on both grape and wine phenolic content. Methyl Jasmonate, chitosan, and a commercial yeast extract were applied to the canopy at veraison and one week later. Results showed that foliar treatments carried out with Methyl Jasmonate and yeast extract achieved the best results, increasing grape and wine anthocyanin content when compared to the control. Moreover, the application of the yeast elicitor also enhanced grape stilbene content. In contrast, the chitosan treatment did not have a substantial impact on the phenolic compounds. The results of this study indicate that Methyl Jasmonate and yeast extract applications could be a simple practice to increase grape and wine phenolic content.

  • Methyl Jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Javier Portu, Pilar Santamaría, Isabel Lopezalfaro, Rosa Lopez, Teresa Gardecerdan
    Abstract:

    The importance of phenolic compounds for grape and wine quality has drawn attention to studying different practices with the aim of increasing their content. Cluster application of elicitors is a viticultural practice that has shown promising results in recent years. However, cluster application requires a previous defoliation, which is time-consuming and expensive. In the present study, Methyl Jasmonate was foliar applied to Tempranillo grapevines in order to study its effect on grape and wine phenolic composition. Methyl Jasmonate foliar application increased anthocyanin and stilbene content in both grape and wine, besides enhancing wine flavonol content. This treatment induced the synthesis of 3-O-glucosides of petunidin and peonidin and trans-p-coumaroyl derivatives of cyanidin and peonidin. For stilbenes, trans-piceid content was considerably increased in both grape and wine. The results obtained suggest that Methyl Jasmonate foliar application could be a simple and accessible practice to enhance gra...