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Nada S Abdelwahab - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of some antiprotozoal drugs in their binary and ternary mixtures with mebeverine hydrochloride in different dosage forms
    Journal of Liquid Chromatography & Related Technologies, 2012
    Co-Authors: Eglal A Abdelaleem, Nada S Abdelwahab
    Abstract:

    A simple, precise, sensitive, and accurate TLC-Densitometric method has been developed and validated for simultaneous determination of the antiprotozoal drugs, Metronidazole Benzoate (METB), and diloxanide furoate (DF) in dimetrol® and furazole® suspensions. Moreover, the method has been optimized to determine Metronidazole (MET), DF, and the antispasmodic drug mebeverine hydrochloride (MEH) in dimetrol® tablets using the same solvent system. Developing system consisting of hexane: acetone: triethyl amine (7: 3: 0.6, by volume) was found to be efficient for good chromatographic separation among the studied drugs using pre-activated silica gel 60 F254 TLC plates. Densitometric quantitation was carried out at 254 nm. The method was successfully applied for determination of MET, DF, MEH, and METB in their bulk powders and in their different dosage forms and the results obtained by applying the proposed TLC-Densitometric method showed no significant difference with those obtained by applying the reported methods.

  • Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods
    Chemistry Central Journal, 2012
    Co-Authors: Eglal A Abdelaleem, Nada S Abdelwahab
    Abstract:

    Background Metronidazole (MET) and Diloxanide Furoate (DF), act as antiprotozoal drugs, in their ternary mixtures with Mebeverine HCl (MEH), an effective antispasmodic drug. This work concerns with the development and validation of two simple, specific and cost effective methods mainly for simultaneous determination of the proposed ternary mixture. In addition, the developed multivariate calibration model has been updated to determine Metronidazole Benzoate (METB) in its binary mixture with DF in Dimetrol^® suspension. Results Method (I) is the mean centering of ratio spectra spectrophotometric method (MCR) that depends on using the mean centered ratio spectra in two successive steps that eliminates the derivative steps and therefore the signal to noise ratio is enhanced. The developed MCR method has been successfully applied for determination of MET, DF and MEH in different laboratory prepared mixtures and in tablets. Method (II) is the partial least square (PLS) multivariate calibration method that has been optimized for determination of MET, DF and MEH in Dimetrol ^® tablets and by updating the developed model, it has been successfully used for prediction of binary mixtures of DF and Metronidazole Benzoate ester (METB) in Dimetrol ^® suspension with good accuracy and precision without reconstruction of the calibration set. Conclusion The developed methods have been validated; accuracy, precision and specificity were found to be within the acceptable limits. Moreover results obtained by the suggested methods showed no significant difference when compared with those obtained by reported methods. Graphical Abstract

  • Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods
    Chemistry Central journal, 2012
    Co-Authors: Eglal A Abdelaleem, Nada S Abdelwahab
    Abstract:

    Metronidazole (MET) and Diloxanide Furoate (DF), act as antiprotozoal drugs, in their ternary mixtures with Mebeverine HCl (MEH), an effective antispasmodic drug. This work concerns with the development and validation of two simple, specific and cost effective methods mainly for simultaneous determination of the proposed ternary mixture. In addition, the developed multivariate calibration model has been updated to determine Metronidazole Benzoate (METB) in its binary mixture with DF in Dimetrol® suspension. Method (I) is the mean centering of ratio spectra spectrophotometric method (MCR) that depends on using the mean centered ratio spectra in two successive steps that eliminates the derivative steps and therefore the signal to noise ratio is enhanced. The developed MCR method has been successfully applied for determination of MET, DF and MEH in different laboratory prepared mixtures and in tablets. Method (II) is the partial least square (PLS) multivariate calibration method that has been optimized for determination of MET, DF and MEH in Dimetrol ® tablets and by updating the developed model, it has been successfully used for prediction of binary mixtures of DF and Metronidazole Benzoate ester (METB) in Dimetrol ® suspension with good accuracy and precision without reconstruction of the calibration set. The developed methods have been validated; accuracy, precision and specificity were found to be within the acceptable limits. Moreover results obtained by the suggested methods showed no significant difference when compared with those obtained by reported methods.

Eglal A Abdelaleem - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of some antiprotozoal drugs in their binary and ternary mixtures with mebeverine hydrochloride in different dosage forms
    Journal of Liquid Chromatography & Related Technologies, 2012
    Co-Authors: Eglal A Abdelaleem, Nada S Abdelwahab
    Abstract:

    A simple, precise, sensitive, and accurate TLC-Densitometric method has been developed and validated for simultaneous determination of the antiprotozoal drugs, Metronidazole Benzoate (METB), and diloxanide furoate (DF) in dimetrol® and furazole® suspensions. Moreover, the method has been optimized to determine Metronidazole (MET), DF, and the antispasmodic drug mebeverine hydrochloride (MEH) in dimetrol® tablets using the same solvent system. Developing system consisting of hexane: acetone: triethyl amine (7: 3: 0.6, by volume) was found to be efficient for good chromatographic separation among the studied drugs using pre-activated silica gel 60 F254 TLC plates. Densitometric quantitation was carried out at 254 nm. The method was successfully applied for determination of MET, DF, MEH, and METB in their bulk powders and in their different dosage forms and the results obtained by applying the proposed TLC-Densitometric method showed no significant difference with those obtained by applying the reported methods.

  • Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods
    Chemistry Central Journal, 2012
    Co-Authors: Eglal A Abdelaleem, Nada S Abdelwahab
    Abstract:

    Background Metronidazole (MET) and Diloxanide Furoate (DF), act as antiprotozoal drugs, in their ternary mixtures with Mebeverine HCl (MEH), an effective antispasmodic drug. This work concerns with the development and validation of two simple, specific and cost effective methods mainly for simultaneous determination of the proposed ternary mixture. In addition, the developed multivariate calibration model has been updated to determine Metronidazole Benzoate (METB) in its binary mixture with DF in Dimetrol^® suspension. Results Method (I) is the mean centering of ratio spectra spectrophotometric method (MCR) that depends on using the mean centered ratio spectra in two successive steps that eliminates the derivative steps and therefore the signal to noise ratio is enhanced. The developed MCR method has been successfully applied for determination of MET, DF and MEH in different laboratory prepared mixtures and in tablets. Method (II) is the partial least square (PLS) multivariate calibration method that has been optimized for determination of MET, DF and MEH in Dimetrol ^® tablets and by updating the developed model, it has been successfully used for prediction of binary mixtures of DF and Metronidazole Benzoate ester (METB) in Dimetrol ^® suspension with good accuracy and precision without reconstruction of the calibration set. Conclusion The developed methods have been validated; accuracy, precision and specificity were found to be within the acceptable limits. Moreover results obtained by the suggested methods showed no significant difference when compared with those obtained by reported methods. Graphical Abstract

  • Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods
    Chemistry Central journal, 2012
    Co-Authors: Eglal A Abdelaleem, Nada S Abdelwahab
    Abstract:

    Metronidazole (MET) and Diloxanide Furoate (DF), act as antiprotozoal drugs, in their ternary mixtures with Mebeverine HCl (MEH), an effective antispasmodic drug. This work concerns with the development and validation of two simple, specific and cost effective methods mainly for simultaneous determination of the proposed ternary mixture. In addition, the developed multivariate calibration model has been updated to determine Metronidazole Benzoate (METB) in its binary mixture with DF in Dimetrol® suspension. Method (I) is the mean centering of ratio spectra spectrophotometric method (MCR) that depends on using the mean centered ratio spectra in two successive steps that eliminates the derivative steps and therefore the signal to noise ratio is enhanced. The developed MCR method has been successfully applied for determination of MET, DF and MEH in different laboratory prepared mixtures and in tablets. Method (II) is the partial least square (PLS) multivariate calibration method that has been optimized for determination of MET, DF and MEH in Dimetrol ® tablets and by updating the developed model, it has been successfully used for prediction of binary mixtures of DF and Metronidazole Benzoate ester (METB) in Dimetrol ® suspension with good accuracy and precision without reconstruction of the calibration set. The developed methods have been validated; accuracy, precision and specificity were found to be within the acceptable limits. Moreover results obtained by the suggested methods showed no significant difference when compared with those obtained by reported methods.

Diana Sober - One of the best experts on this subject based on the ideXlab platform.

  • stability indicating reversed phase liquid chromatographic determination of Metronidazole Benzoate and diloxanide furoate as bulk drug and in suspension dosage form
    Journal of Pharmaceutical and Biomedical Analysis, 2005
    Co-Authors: Adel Mishal, Diana Sober
    Abstract:

    Abstract A selective, precise and accurate isocratic RP-HPLC method has been developed and validated for the simultaneous determination of Metronidazole Benzoate (MB), diloxanide furoate (DF), methyl paraben (MPn) and propyl paraben (PPn) in suspension. The cis / trans isomerization phenomenon for DF has also been presented. The method uses as stationary phase a Supelco LC-18 DB (15 cm × 4.6 mm) 5 μm column and as a mobile phase a buffer-acetonitrile mixture (70:30, v/v) adjusted to pH of 2.5 at a flow rate of 2.0 mL min −1 . The buffer is a 0.005 M KH 2 PO 4 solution. The four analytes were well resolved from the degraded solutions peaks. The excepients present in the formulation do not interfere with the assay procedure. The linearity range ( n  = 3) is (0.20130–1.20779 mg mL −1 ) for MB with R of 0.99985; (0.15790–0.94740 mg mL −1 ) for DF with R of 0.99987; (0.01131–0.06788 mg mL −1 ) for MPn with R of 0.99987 and (0.00126–0.00756 mg mL −1 ) for PPn with R of 0.99991. Precision ( n  = 6) was 0.87% for MB; 1.15% for DF; 1.32% for MPn and 1.27% for PPn. The percentage recoveries ( n  = 3) were 99.1% for MB; 99.6% for DF; 99.1% for MPn and 98.7% for PPn. The proposed method can be utilized for the routine analysis of the four analytes in pharmaceutical dosage form.

Mahmoud A Takieddin - One of the best experts on this subject based on the ideXlab platform.

  • Simultaneous Determination of Metronidazole Benzoate Methylparaben, and Propylparaben by High-Performance Liquid Chromatography
    Drug development and industrial pharmacy, 1999
    Co-Authors: Mohammed Shahid Ali, Rakesh Singh Chaudhary, Mahmoud A Takieddin
    Abstract:

    The simultaneous determination of Metronidazole Benzoate (MB), methylparaben (MP), and propylparaben (PP) in an oral suspension formulation was developed using high-performance liquid chromatography (HPLC). The method was developed using a Novapak C18 (3.9 × 150 mm, 4 μm) column, methanol-water (50:50, v/v) as the mobile phase and an ultraviolet (UV) detector at 254 nm. The peak area response versus concentration was linear in a concentration range from 40 to 400 μg/ml of MB, 0.8 to 8.0 μg/ml of MP, and 0.2 to 2.0 μg of PP. The correlation coefficients were 0.9997 for MB, 0.9987 for MP, and 0.9983 for PP, with relative standard errors of 1.12%, 1.28%, and 1.67%, respectively.

Adel Mishal - One of the best experts on this subject based on the ideXlab platform.

  • stability indicating reversed phase liquid chromatographic determination of Metronidazole Benzoate and diloxanide furoate as bulk drug and in suspension dosage form
    Journal of Pharmaceutical and Biomedical Analysis, 2005
    Co-Authors: Adel Mishal, Diana Sober
    Abstract:

    Abstract A selective, precise and accurate isocratic RP-HPLC method has been developed and validated for the simultaneous determination of Metronidazole Benzoate (MB), diloxanide furoate (DF), methyl paraben (MPn) and propyl paraben (PPn) in suspension. The cis / trans isomerization phenomenon for DF has also been presented. The method uses as stationary phase a Supelco LC-18 DB (15 cm × 4.6 mm) 5 μm column and as a mobile phase a buffer-acetonitrile mixture (70:30, v/v) adjusted to pH of 2.5 at a flow rate of 2.0 mL min −1 . The buffer is a 0.005 M KH 2 PO 4 solution. The four analytes were well resolved from the degraded solutions peaks. The excepients present in the formulation do not interfere with the assay procedure. The linearity range ( n  = 3) is (0.20130–1.20779 mg mL −1 ) for MB with R of 0.99985; (0.15790–0.94740 mg mL −1 ) for DF with R of 0.99987; (0.01131–0.06788 mg mL −1 ) for MPn with R of 0.99987 and (0.00126–0.00756 mg mL −1 ) for PPn with R of 0.99991. Precision ( n  = 6) was 0.87% for MB; 1.15% for DF; 1.32% for MPn and 1.27% for PPn. The percentage recoveries ( n  = 3) were 99.1% for MB; 99.6% for DF; 99.1% for MPn and 98.7% for PPn. The proposed method can be utilized for the routine analysis of the four analytes in pharmaceutical dosage form.