Microarrays

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Hans-peter Saluz - One of the best experts on this subject based on the ideXlab platform.

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical Chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their co...

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their commercial genotyping array counterparts.

Florian Erfurth - One of the best experts on this subject based on the ideXlab platform.

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical Chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their co...

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their commercial genotyping array counterparts.

Lucia Mosiello - One of the best experts on this subject based on the ideXlab platform.

  • an antibody based microarray assay for the simultaneous detection of aflatoxin b1 and fumonisin b1
    Mycotoxin Research, 2009
    Co-Authors: Ilaria Lamberti, C Tanzarella, Isabella Solinas, Cristiano Padula, Lucia Mosiello
    Abstract:

    Advances in microsystem technology have enabled protein and nucleic acid-based Microarrays to be used in various applications, including the study of diseases, drug discovery, genetic screening, and clinical and food diagnostics. Analytical methods for the detection of mycotoxins, however, remain largely based on thin layer chromatography (TLC), high pressure liquid chromatography (HPLC), or enzyme-linked Immunosorbent assay (ELISA) . The aim of our work, therefore, was to transfer an immunological assay from microtitrr plates into microarray format, in order to develop a multiparametric, rapid, sensitive and inexpensive method for the detection of mycotoxins for use in food safety applications. Microarray technology enables the fast and parallel analysis of a multitude of biologically relevant parameters. Not only nucleic acid-based tests but also peptide, antigen, and antibody assays, using different formats of Microarrays, have evolved within the last decade. Antibody-based Microarrays provide a powerful tool that can be used to generate rapid and detailed expression profiles of a defined set of analytes in complex samples and are potentially useful for generating rapid immunological assays of food contaminants. In this paper, we report a feasibility study of the application of antibody Microarrays for the simultaneous (or independent) detection of two common mycotoxins, Aflatoxin B1 and Fumonisin B1. We present the development of microarray detection of aflatoxin B1 and fumonisin B1 in standard solutions with detection limits of 3 ng/ml of AFB1 and 43 ng/ml for FB1, and have developed a competitive immunoassay in microarray format for simultaneous analyses. The quality of the microarray data is comparable to data generated by microplate-based immunoassay (ELISA), but further investigations are needed in order to characterise our method more fully. We hope that these preliminary results might suggest that further research is warranted in order to develop hapten Microarrays for the immunochemical simultaneous analysis of mycotoxins, as well as for other small molecules (e.g. bacterial toxins or biological warfare agents).

  • An antibody-based microarray assay for the simultaneous detection of aflatoxin B_1 and fumonisin B_1
    Mycotoxin Research, 2009
    Co-Authors: Ilaria Lamberti, C Tanzarella, Isabella Solinas, Cristiano Padula, Lucia Mosiello
    Abstract:

    Advances in microsystem technology have enabled protein and nucleic acid-based Microarrays to be used in various applications, including the study of diseases, drug discovery, genetic screening, and clinical and food diagnostics. Analytical methods for the detection of mycotoxins, however, remain largely based on thin layer chromatography (TLC), high pressure liquid chromatography (HPLC), or enzyme-linked Immunosorbent assay (ELISA) . The aim of our work, therefore, was to transfer an immunological assay from microtitrr plates into microarray format, in order to develop a multiparametric, rapid, sensitive and inexpensive method for the detection of mycotoxins for use in food safety applications. Microarray technology enables the fast and parallel analysis of a multitude of biologically relevant parameters. Not only nucleic acid-based tests but also peptide, antigen, and antibody assays, using different formats of Microarrays, have evolved within the last decade. Antibody-based Microarrays provide a powerful tool that can be used to generate rapid and detailed expression profiles of a defined set of analytes in complex samples and are potentially useful for generating rapid immunological assays of food contaminants. In this paper, we report a feasibility study of the application of antibody Microarrays for the simultaneous (or independent) detection of two common mycotoxins, Aflatoxin B_1 and Fumonisin B_1. We present the development of microarray detection of aflatoxin B1 and fumonisin B1 in standard solutions with detection limits of 3 ng/ml of AFB1 and 43 ng/ml for FB1, and have developed a competitive immunoassay in microarray format for simultaneous analyses. The quality of the microarray data is comparable to data generated by microplate-based immunoassay (ELISA), but further investigations are needed in order to characterise our method more fully. We hope that these preliminary results might suggest that further research is warranted in order to develop hapten Microarrays for the immunochemical simultaneous analysis of mycotoxins, as well as for other small molecules (e.g. bacterial toxins or biological warfare agents).

Alexander Tretyakov - One of the best experts on this subject based on the ideXlab platform.

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical Chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their co...

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their commercial genotyping array counterparts.

Dieter Fassler - One of the best experts on this subject based on the ideXlab platform.

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical Chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their co...

  • Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor Microarrays.
    Analytical chemistry, 2008
    Co-Authors: Florian Erfurth, Berla Dipl.-ing Nyuyki, Grit Mrotzek, Wolf-dieter Schmidt, Dieter Fassler, Alexander Tretyakov, Hans-peter Saluz
    Abstract:

    We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping Microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled Microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of Microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color Microarrays containing dyes with spectral properties similar to their commercial genotyping array counterparts.

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