The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Clea Bauvais - One of the best experts on this subject based on the ideXlab platform.
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sponging up metals bacteria associated with the marine sponge spongia officinalis
Marine Environmental Research, 2015Co-Authors: Clea Bauvais, Severine Zirah, Laurie Piette, Florence Chaspoul, Isabelle Domartcoulon, Virginie Chapon, Philippe Gallice, Sylvie Rebuffat, Thierry PerezAbstract:The present study explored the bacteria of the sponge Spongia officinalis in a metal-polluted environment, using PCR-DGGE fingerprinting, culture-dependent approaches and in situ hybridization. The sponge samples collected over three consecutive years in the Western Mediterranean Sea contained high concentrations of zinc, nickel, lead and copper determined by ICP-MS. DGGE signatures indicated a sponge specific bacterial association and suggested spatial and temporal variations. The bacterial culturable fraction associated with S. officinalis and tolerant to heavy metals was isolated using metal-enriched Microbiological Media. The obtained 63 aerobic strains were phylogenetically affiliated to the phyla Proteobacteria, Actinobacteria, and Firmicutes. All isolates showed high tolerances to the selected heavy metals. The predominant genus Pseudovibrio was localized via CARD-FISH in the sponge surface tissue and validated as a sponge-associated epibiont This study is the first step in understanding the potential involvement of the associated bacteria in sponge's tolerance to heavy metals. (C) 2014 Elsevier Ltd. All rights reserved.
Thierry Perez - One of the best experts on this subject based on the ideXlab platform.
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sponging up metals bacteria associated with the marine sponge spongia officinalis
Marine Environmental Research, 2015Co-Authors: Clea Bauvais, Severine Zirah, Laurie Piette, Florence Chaspoul, Isabelle Domartcoulon, Virginie Chapon, Philippe Gallice, Sylvie Rebuffat, Thierry PerezAbstract:The present study explored the bacteria of the sponge Spongia officinalis in a metal-polluted environment, using PCR-DGGE fingerprinting, culture-dependent approaches and in situ hybridization. The sponge samples collected over three consecutive years in the Western Mediterranean Sea contained high concentrations of zinc, nickel, lead and copper determined by ICP-MS. DGGE signatures indicated a sponge specific bacterial association and suggested spatial and temporal variations. The bacterial culturable fraction associated with S. officinalis and tolerant to heavy metals was isolated using metal-enriched Microbiological Media. The obtained 63 aerobic strains were phylogenetically affiliated to the phyla Proteobacteria, Actinobacteria, and Firmicutes. All isolates showed high tolerances to the selected heavy metals. The predominant genus Pseudovibrio was localized via CARD-FISH in the sponge surface tissue and validated as a sponge-associated epibiont This study is the first step in understanding the potential involvement of the associated bacteria in sponge's tolerance to heavy metals. (C) 2014 Elsevier Ltd. All rights reserved.
Michael P Davidson - One of the best experts on this subject based on the ideXlab platform.
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plant essential oils and components on growth of spoilage yeasts in Microbiological Media and a model salad dressing
Food Control, 2016Co-Authors: Emefa A Monu, Chayapa Techathuvanan, Audra Wallis, Faith Critzer, Michael P DavidsonAbstract:Abstract Essential oils (EOs) and EO components have been widely tested for their antimicrobial properties against microorganisms. However, less is known about the inhibitory properties of essential oil components against microorganisms in foods and, more specifically, spoilage yeasts in a food matrix. Clove bud, cinnamon bark, and thyme oils, and the EO components, trans-cinnamaldehyde, cinnamic acid, eugenol, carvacrol, and thymol first were evaluated for their antimicrobial activity against yeasts in Microbiological Media. The yeasts tested included Torulaspora delbrueckii, Candida krusei, Schizosaccharomyces pombe, and Zygosaccharomyces bailii. The most efficacious EO components in Media were applied to a model mayonnaise-based salad dressing as a food model to determine the effect on spoilage yeast growth. Trans-cinnamaldehyde and cinnamon bark oil were the most effective against yeasts in Microbiological Media among the compounds tested with minimum inhibitory concentrations (MICs) of 50 mg/L. Thymol and carvacrol had the next most inhibitory activity against yeasts with MICs of 200 mg/L. In a model salad dressing at pH 4.2, trans-cinnamaldehyde at 500 mg/L was most effective among the EO components inhibiting S. pombe and Z. bailii during 4- and 5-day storage, respectively, at 22 °C.
Luca Cocolin - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling
2016Co-Authors: Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca CocolinAbstract:In this study, the fecal microbiota of 153 healthy volunteers, recruited from four different lo-cations in Italy, has been studied by coupling viable counts, on different Microbiological Media, with ribosomal RNA Denaturing Gradient Gel Electrophoresis (rRNA-DGGE). The volunteers followed three different diets, namely omnivore, ovo-lacto-vegetarian and vegan. The results obtained from culture-dependent and-independent methods have underlined a high level of similarity of the viable fecal microbiota for the three investigated diets. The rRNA DGGE profiles were very complex and comprised a total number of bands that varied from 67 to 64 for the V3 and V9 regions of the 16S rRNA gene, respectively. Only a few bands were specific in/of all three diets, and the presence of common taxa associated with the dietary habits was found. As far as the viable counts are concerned, the high similarity of the fecal microbiota was once again confirmed, with only a few of the investigated groups showing significant differences. Interestingly, the samples grouped differently, according to the recruitment site, thus highlighting a higher impact of the food consumed by the volun-teers in the specific geographical locations than that of the type of diet. Lastly, it should b
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Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling
2015Co-Authors: Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca CocolinAbstract:In this study, the fecal microbiota of 153 healthy volunteers, recruited from four different locations in Italy, has been studied by coupling viable counts, on different Microbiological Media, with ribosomal RNA Denaturing Gradient Gel Electrophoresis (rRNA-DGGE). The volunteers followed three different diets, namely omnivore, ovo-lacto-vegetarian and vegan. The results obtained from culture-dependent and -independent methods have underlined a high level of similarity of the viable fecal microbiota for the three investigated diets. The rRNA DGGE profiles were very complex and comprised a total number of bands that varied from 67 to 64 for the V3 and V9 regions of the 16S rRNA gene, respectively. Only a few bands were specific in/of all three diets, and the presence of common taxa associated with the dietary habits was found. As far as the viable counts are concerned, the high similarity of the fecal microbiota was once again confirmed, with only a few of the investigated groups showing significant differences. Interestingly, the samples grouped differently, according to the recruitment site, thus highlighting a higher impact of the food consumed by the volunteers in the specific geographical locations than that of the type of diet. Lastly, it should be mentioned that the fecal microbiota DGGE profiles obtained from the DNA were clearly separated from those produced using RNA, thus underlining a difference between the total and viable populations in the fecal samples.
Patrick Videau - One of the best experts on this subject based on the ideXlab platform.
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Assessment and verification of commercially available pressure cookers for laboratory sterilization.
PloS one, 2018Co-Authors: Vaille Swenson, Amanda D. Stacy, Michael O. Gaylor, Blake Ushijima, Benjamin Philmus, Loralyn M. Cozy, Nina M. Videau, Patrick VideauAbstract:Laboratory science requires careful maintenance of sterile reagents and tools as well as the sterilization of waste prior to disposal. However, steam autoclaves typically used for this purpose may not be readily accessible to everyone in the scientific community, such as K-12 teachers, researchers in the field, students in under-funded laboratories, or persons in the developing world who lack funding and resources. This work examines the use of commercial electric pressure cookers as an alternative method for the sterilization of Media, instruments, and waste. Four commonly available brands of pressure cooker were tested for their ability to sterilize Microbiological Media, a variety of metal instruments, and high-titer microbial cultures. All four pressure cookers were able to sterilize these starting materials as well as a range of microbial types, including Gram-positive bacteria, Gram-negative bacteria, filamentous fungi, unicellular fungi, and mixed environmental samples. Only the Instant Pot, however, was able to sterilize autoclave tester ampoules of Geobacillus stearothermophilus spores. These results suggest that, depending on the nature of the work undertaken, store-bought pressure cookers can be an appropriate substitute for commercial autoclaves. Their adoption may also help increase the accessibility of science to a broader range of investigators.