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Wonbo Shim - One of the best experts on this subject based on the ideXlab platform.
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A Conserved Homeobox Transcription Factor Htf1 Is Required for Phialide Development and Conidiogenesis in Fusarium Species
2016Co-Authors: Wenhui Zheng, Qingping Huang, Chengkang Zhang, Huanchen Zhai, Wonbo Shim, Xu Zhao, Qiurong Xie, Zonghua WangAbstract:Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- Microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical Microconidia chains were absent in F. verticillioides mutants. F. graminearum an
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a conserved homeobox transcription factor htf1 is required for phialide development and conidiogenesis in fusarium species
PLOS ONE, 2012Co-Authors: Wenhui Zheng, Qingping Huang, Chengkang Zhang, Huanchen Zhai, Liping Xu, Wonbo Shim, Guodong Lu, Xu ZhaoAbstract:Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- Microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical Microconidia chains were absent in F. verticillioides mutants. F. graminearum and F. verticillioides mutants were complemented by introducing its native HTF1 gene or homologs from other Fusarium species. These results suggest that Fusarium Htf1 is functionally conserved homeobox transcription factor that regulates phialide development and conidiogenesis via distinct signaling pathways yet to be characterized in fungi.
Muñoz Gómez Amalia - One of the best experts on this subject based on the ideXlab platform.
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Estudio de la interacción molecular huésped-patógeno utilizando el modelo insecto-hongo gallería mellonella-fusarium oxysporum, mediante la caracterización de genes, proteínas y péptidos de defensa provenientes de la respuesta humoral innata y del ataque fúngico
Facultad de Ciencias Farmacéuticas y Alimentarias, 2015Co-Authors: Muñoz Gómez AmaliaAbstract:Se estudiaron aspectos involucrados en el ataque del hongo Fusarium oxysporum a larvas de Galleria mellonella (Lepidóptera), y el proceso de defensa que se desencadena en el sistema inmune innato del huésped. Los insectos responden al desafío de agentes microbianos o a lesiones, mediante la rápida y transitoria síntesis de potentes compuestos antimicrobianos. Numerosas proteínas del sistema inmune provenientes de insectos que inhiben microorganismos han sido identificadas caracterizadas y clonadas in vitro, la mayor parte de las cuales exhiben actividad antibacteriana, mientras que en otras se demostró que afectaban a hongos. Los genes más estudiados de F. oxysporum son aquellos que producen toxinas, y en el caso de G. mellonella, son muy pocos los genes identificados, provenientes de su respuesta inmune innata. Para ambas especies es aún muy somera la caracterización de sus respectivos proteomas. Para la comprensión de la relación huésped patógeno (insecto-hongo), las estrategias metodológicas seguidas incluyeron, la estandarización de la colonia de G. mellonella y del proceso de inoculación del hongo en larvas del insecto; la identificación de temperaturas óptimas de mantenimiento de las larvas y las concentraciones sub-letales de Microconidias del hongo donde se produjo la mayor supervivencia de las larvas; el empleo de la tecnología iTRAQ (Isobaric tags for relative and absolute quantitation). Esta última permitió identificar y cuantificar las proteínas expresadas, además, mediante la utilización de ARN mensajero se identificaron genes expresados en condiciones deseadas. Finalmente, se llevó a cabo la identificación in vivo de células activas de las larvas atacadas por F. oxysporum, mediante microscopía láser confocal...In this work it was investigated different molecular and physiological aspects related with attack of pathogen Fusarium oxysporum (fungus) and the immune defense of Galleria mellonella (Lepidoptera). The challenge of insect is to reply the attack of microbial pathogens or make the improvements to the lesions, producing rapidly and transitory a large number of proteins and antimicrobial compounds. Many proteins and peptides of innate immune system from insect that inhibit microorganisms, were previously in vitro identified, characterized and cloned, most of them has antibacterial activity, while another ones are related with fungi defense. The most studied genes in F. oxysporum are the responsible of toxins. For the case of G. mellonella not so much genes were identified and the immune system genes are essentially poorly studied. Both organisms are poorly studied to the proteomics level. For the best comprehension of the relationship host-pathogen (insect-fungus), the methodological strategy was follow the next steps: G. mellonella colony culture standardization, the right procedure of injection of fungus Microconidia into insect, the identification of the best temperature for the maintenance of larvae, the best sublethal Microconidia concentration for producing more larvae survivors and the usefulness of iTRAQ technology (Isobaric Tags for Relative and Absolute Quantitation). This last technique allowed identify and quantify a large number of expressed proteins (up or down regulated), furthermore, isolating mRNA those proteins identified were correlated with gene expression under suitable conditions. Finally, It was carry out the in vivo identification of hemocoel active cells from larvae challenged F. oxysporum, by confocal microscopy. It was stablished by fungus radial grown, where F. oxysporum grows in correlation with temperature, exhibiting low grow at 37 that at 30°C. The larvae survive in best proportion to the challenge of Microconidia from 104 to 106 Microconidia/mL, when were incubated at 37°C, during 48 h post-infection, and present best surviving when were pre-inoculated with Latobacillus platarum. Also, it was observed cellular differentiation into hemolymph larvae with fungus- challenge. Using iTRAQ 8-plex were identify 59 proteins, which 20 belong to G. mellonella, 20 were identified using other lepidotera species and the remained were identified using other species from bacteria, protozoan and fungus. In conclusion, the standardization of breeding system, the handling and the injection of G. mellonella larvae with F. oxysporum Microconidia were reliable and consistent, as well as the maintenance and culture of F. oxysporum was meticulous. The larvae survived with high Microconidia/mL concentration, when the temperature incubation was 37°C, especially when the larvae were pre-inoculated with L. plantarum, before the fungus challenge. iTRAQ was a reliable method and consistent for detecting proteins related to innate immune system from G. mellonella in response to the pathogen F. oxysporum. In fact, iTRAQ as a regard of quantitative proteomics analytic method allowed quantify protein and and peptides, therefore allow to study their differential expression and improvement of the immune system from G. mellonella through the treatmet from the low to high of Microconidia concentration and from the 25 to 37°C. Our proteomic study allowed following the improvement of immune response in G. mellonella against to fungus challenge at 37°C. In conclusion it was found that using 104 Microconidia/mL at 37°C, over-expressed much more proteins than other treatments
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Estudio de la interacción molecular huésped-patógeno utilizando el modelo insecto-hongo gallería mellonella-fusarium oxysporum, mediante la caracterización de genes, proteínas y péptidos de defensa provenientes de la respuesta humoral innata y del ataque fúngico
Medellín Colombia, 2015Co-Authors: Muñoz Gómez AmaliaAbstract:RESUMEN: Se estudiaron aspectos involucrados en el ataque del hongo Fusarium oxysporum a larvas de Galleria mellonella (Lepidóptera), y el proceso de defensa que se desencadena en el sistema inmune innato del huésped. Los insectos responden al desafío de agentes microbianos o a lesiones, mediante la rápida y transitoria síntesis de potentes compuestos antimicrobianos. Numerosas proteínas del sistema inmune provenientes de insectos que inhiben microorganismos han sido identificadas caracterizadas y clonadas in vitro, la mayor parte de las cuales exhiben actividad antibacteriana, mientras que en otras se demostró que afectaban a hongos. Los genes más estudiados de F. oxysporum son aquellos que producen toxinas, y en el caso de G. mellonella, son muy pocos los genes identificados, provenientes de su respuesta inmune innata. Para ambas especies es aún muy somera la caracterización de sus respectivos proteomas. Para la comprensión de la relación huésped patógeno (insecto-hongo), las estrategias metodológicas seguidas incluyeron, la estandarización de la colonia de G. mellonella y del proceso de inoculación del hongo en larvas del insecto; la identificación de temperaturas óptimas de mantenimiento de las larvas y las concentraciones sub-letales de Microconidias del hongo donde se produjo la mayor supervivencia de las larvas; el empleo de la tecnología iTRAQ (Isobaric tags for relative and absolute quantitation). Esta última permitió identificar y cuantificar las proteínas expresadas, además, mediante la utilización de ARN mensajero se identificaron genes expresados en condiciones deseadas. Finalmente, se llevó a cabo la identificación in vivo de células activas de las larvas atacadas por F. oxysporum, mediante microscopía láser confocal...ABSTRACT:In this work it was investigated different molecular and physiological aspects related with attack of pathogen Fusarium oxysporum (fungus) and the immune defense of Galleria mellonella (Lepidoptera). The challenge of insect is to reply the attack of microbial pathogens or make the improvements to the lesions, producing rapidly and transitory a large number of proteins and antimicrobial compounds. Many proteins and peptides of innate immune system from insect that inhibit microorganisms, were previously in vitro identified, characterized and cloned, most of them has antibacterial activity, while another ones are related with fungi defense. The most studied genes in F. oxysporum are the responsible of toxins. For the case of G. mellonella not so much genes were identified and the immune system genes are essentially poorly studied. Both organisms are poorly studied to the proteomics level. For the best comprehension of the relationship host-pathogen (insect-fungus), the methodological strategy was follow the next steps: G. mellonella colony culture standardization, the right procedure of injection of fungus Microconidia into insect, the identification of the best temperature for the maintenance of larvae, the best sublethal Microconidia concentration for producing more larvae survivors and the usefulness of iTRAQ technology (Isobaric Tags for Relative and Absolute Quantitation). This last technique allowed identify and quantify a large number of expressed proteins (up or down regulated), furthermore, isolating mRNA those proteins identified were correlated with gene expression under suitable conditions. Finally, It was carry out the in vivo identification of hemocoel active cells from larvae challenged F. oxysporum, by confocal microscopy. It was stablished by fungus radial grown, where F. oxysporum grows in correlation with temperature, exhibiting low grow at 37 that at 30°C. The larvae survive in best proportion to the challenge of Microconidia from 104 to 106 Microconidia/mL, when were incubated at 37°C, during 48 h post-infection, and present best surviving when were pre-inoculated with Latobacillus platarum. Also, it was observed cellular differentiation into hemolymph larvae with fungus- challenge. Using iTRAQ 8-plex were identify 59 proteins, which 20 belong to G. mellonella, 20 were identified using other lepidotera species and the remained were identified using other species from bacteria, protozoan and fungus. In conclusion, the standardization of breeding system, the handling and the injection of G. mellonella larvae with F. oxysporum Microconidia were reliable and consistent, as well as the maintenance and culture of F. oxysporum was meticulous. The larvae survived with high Microconidia/mL concentration, when the temperature incubation was 37°C, especially when the larvae were pre-inoculated with L. plantarum, before the fungus challenge. iTRAQ was a reliable method and consistent for detecting proteins related to innate immune system from G. mellonella in response to the pathogen F. oxysporum. In fact, iTRAQ as a regard of quantitative proteomics analytic method allowed quantify protein and and peptides, therefore allow to study their differential expression and improvement of the immune system from G. mellonella through the treatmet from the low to high of Microconidia concentration and from the 25 to 37°C. Our proteomic study allowed following the improvement of immune response in G. mellonella against to fungus challenge at 37°C. In conclusion it was found that using 104 Microconidia/mL at 37°C, over-expressed much more proteins than other treatments
Huanchen Zhai - One of the best experts on this subject based on the ideXlab platform.
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A Conserved Homeobox Transcription Factor Htf1 Is Required for Phialide Development and Conidiogenesis in Fusarium Species
2016Co-Authors: Wenhui Zheng, Qingping Huang, Chengkang Zhang, Huanchen Zhai, Wonbo Shim, Xu Zhao, Qiurong Xie, Zonghua WangAbstract:Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- Microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical Microconidia chains were absent in F. verticillioides mutants. F. graminearum an
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a conserved homeobox transcription factor htf1 is required for phialide development and conidiogenesis in fusarium species
PLOS ONE, 2012Co-Authors: Wenhui Zheng, Qingping Huang, Chengkang Zhang, Huanchen Zhai, Liping Xu, Wonbo Shim, Guodong Lu, Xu ZhaoAbstract:Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- Microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical Microconidia chains were absent in F. verticillioides mutants. F. graminearum and F. verticillioides mutants were complemented by introducing its native HTF1 gene or homologs from other Fusarium species. These results suggest that Fusarium Htf1 is functionally conserved homeobox transcription factor that regulates phialide development and conidiogenesis via distinct signaling pathways yet to be characterized in fungi.
Wenhui Zheng - One of the best experts on this subject based on the ideXlab platform.
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A Conserved Homeobox Transcription Factor Htf1 Is Required for Phialide Development and Conidiogenesis in Fusarium Species
2016Co-Authors: Wenhui Zheng, Qingping Huang, Chengkang Zhang, Huanchen Zhai, Wonbo Shim, Xu Zhao, Qiurong Xie, Zonghua WangAbstract:Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- Microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical Microconidia chains were absent in F. verticillioides mutants. F. graminearum an
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a conserved homeobox transcription factor htf1 is required for phialide development and conidiogenesis in fusarium species
PLOS ONE, 2012Co-Authors: Wenhui Zheng, Qingping Huang, Chengkang Zhang, Huanchen Zhai, Liping Xu, Wonbo Shim, Guodong Lu, Xu ZhaoAbstract:Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- Microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical Microconidia chains were absent in F. verticillioides mutants. F. graminearum and F. verticillioides mutants were complemented by introducing its native HTF1 gene or homologs from other Fusarium species. These results suggest that Fusarium Htf1 is functionally conserved homeobox transcription factor that regulates phialide development and conidiogenesis via distinct signaling pathways yet to be characterized in fungi.
Priscila Chaverri - One of the best experts on this subject based on the ideXlab platform.
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Phylogeny and taxonomic revision of Thelonectria discophora (Ascomycota, Hypocreales, Nectriaceae) species complex
Fungal Diversity, 2015Co-Authors: Catalina Salgado-salazar, Amy Y. Rossman, Gary J. Samuels, Yuuri Hirooka, Romina M. Sanchez, Priscila ChaverriAbstract:Specimens regarded as Thelonectria discophora ( Thelonectria , Nectriaceae , Hypocreales ) constitute a conspicuous group of saprobic fungi on decaying plant material, characterized by red perithecia each with a broad mammiform (nipple-like) apex. The asexual state is characterized by a cylindrocarpon-like morphology, with 3–5 septate macroconidia, unicellular Microconidia and chlamydospores that are rarely produced in culture. In the past, T. discophora was regarded as one species with a wide geographic distribution. However, a recent study rejected the monophyly and cosmopolitan distribution of this species , and showed the existence of at least 16 cryptic species distributed in three main groups. By combining the results of phylogenetic analyses of six nuclear loci and morphological studies, we revised the taxonomy of the T. discophora species complex, resulting in the description of 12 new species and four new combinations based on historic names. Even though molecular phylogenetic analyses strongly support the segregation of these species, and are in agreement with previous studies, individual diagnostic morphological characters for each species could not be identified. However, discrete morphological traits corresponding to each of the three main groups of species were discovered. Lineages could be differentiated based on the average values of morphological traits as well as the presence/absence of characteristic asexual propagules and colony growth at 30C. Descriptions, illustrations are provided for the recognized species.
